Tumor Dissociation Kit, human from Miltenyi Biotec

(1) IL-12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancerMolecular Therapy AdvancesJune 11, 2026Lupita S. Lopez, Ziyou Cui, Yukiko Yamaguchi, John P. Murad, Zhiyuan Yang, Ke Zou, Jason Yang, Wen-Chung Chang, Stephen J. Forman, Vivien W. Chan, et al.single-cell suspensions were prepared using the Miltenyi Biotec human tumor dissociation kit (cat: 130-095-929) per the manufacturer’s protocol. LAPC-9 cells were transduced with lentivirus to express enhanced green

(2) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-Ludlowdiameter: 10 cm, surface: standard",Sarstedt,Cat#83.3902 "Tumor Dissociation Kit, human",Miltenyi,Cat#130-095-929; RRID:SCR_020276 gentleMACS™ C Tubes,Miltenyi,Cat#130-093-237; RRID:SCR_020270 gentleMACS™ Dissociator,Miltenyi,Cat#130-093-235;

(3) Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancerNat CommunMay 11, 2026Yasaman Setayeshpour, Ssu-Yu Chen, Divya L. Dayanidhi, Yunji Lee, Shao-Chin Wu, Juan J. Aristizabal-Henao, Jianli Wu, Chao-Chieh Lin, Nazanin Setayeshpour, Chiara Federico, et al.manufacturer-recommended amounts of H, R, and A enzymes in the Tumor Dissociation Kit, human (Miltenyi Biotec, 130-095-929). Samples were digested for 60 min at 37 °C in the Roto-Therm Plus (Ward’s Science). Cells and tissue

(4) Modelling kidney cystogenesis using human kidney tubuloid culturesBMC Mol Cell BiolMay 11, 2026Jana Rohe, Arsila Palliyulla Kariat Ashraf, Melina Kehl, Xenia Thielmann, Jörg Ellinger, Glen Kristiansen, Dagmar Wachten, Marieta Ioana Tomadissociated using gentleMACS™ Octo Dissociator and the Tumor Dissociation Kit, human (Miltenyi Biotec, #130-095-929). The dissociated cells were passed through a 100 μm strainer (Corning, #431752), resuspended in the

(5) Metastatic organotropism in small cell lung cancerbioRxivApril 30, 2026Manan Krishnamurthy, Anjali Dhall, Sarthak Sahoo, Christopher W. Schultz, Michelle A. Baird, Parth Desai, Jacob Odell, Nobuyuki Takahashi, Michael Nirula, Sophie Zhuang, et al.were rapidly transferred to lab and dissociated using Tumor Dissociation kit, human (Miltenyi Biotec 130-095-929) and gentleMACS Dissociator on 37°C hTDK-1 pre-set protocol. Dissociated cells were then used for scRNAseq

(6) Systematic design of combination therapy by targeting master regulators of coexisting diffuse midline glioma cell statesNature GeneticsApril 22, 2026Ester Calvo Fernández, Lorenzo Tomassoni, Xu Zhang, Junqiang Wang, Aleksandar Obradovic, Pasquale Laise, Aaron T. Griffin, Lukas Vlahos, Hanna E. Minns, Diana V. Morales, et al.μl), Enzyme R (100 μl) and Enzyme A (25 μl) from the Human Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929). Samples were dissociated using the gentleMACS Dissociator (‘Medium’ tumor program; 37C_h_TDK_2) for

(7) Preventing trogocytosis by cathepsin B inhibition augments CAR T-cell functionSignal Transduct Target TherApril 22, 2026Kenneth A. Dietze, Kiet Nguyen, Aashli Pathni, Frank Fazekas, Wenxiang Sun, Ethan Rosati, Jillian M. Baker, Maday Galeana Figueroa, Etse Gebru, Daniel Yamoah, et al.tumors were collected and dissociated using the Human Tumor Dissociation Kit (Miltenyi Biotec, cat # 130-095-929) and the OctoMACS tissue dissociator (Miltenyi Biotec). CAR T cells were quantified and phenotyped by

(8) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.washed in PBS (Thermo Fisher) and dissociated using a Human Tumor Dissociation Kit (Miltenyi Biotec #130-095-929) per manufacturer guidelines. Samples were dissociated at 37°C for 1 h on a gentleMACS Octo Dissociator

(9) The landscape of responses to neoadjuvant immunotherapy in resectable Kirsten rat sarcoma viral oncogene homolog-mutant lung adenocarcinoma: Clinical heterogeneity and correlative immunologic analysisClin Transl MedApril 20, 2026Sikai Wu, Jiheng Niu, Xiaowei Chen, Jinfei Li, Quanying Tang, Zhenlin Yang, Shugeng Gaowere enzymatically digested for 60 min at 37°C on a rotator using a Tumour Dissociation Kit (Miltenyi, 130‐095‐929). The digested suspension was subsequently filtered through a 100 µm MACS SmartStrainer (Miltenyi, 130‐110‐917)

(10) Stemness and Survival: CD117+/CD133+ Subpopulations Sustain PI3K Signaling and Drive Imatinib Resistance in Head and Neck Mucosal MelanomaCellsApril 19, 2026Sofie-Yasmin Hassan, Simeon Santourlidis, Thomas W. Flanagan, Sarah-Lilly Hassan, He Zhou, Morna F. Schmidt, Claudio Cacchi, Matthias Ferdinand Lammert, Mossad Megahed, Amir Sadegh Yazdi, et al.suspension using mechanical mincing and enzymatic digestion using the human Tumor Dissociation Kit MACS (#130-095-929) Miltenyi Biotech, Mönchengladbach, Germany, as described [19,39], followed by Magnetic Depletion after

(11) Proteome profiles of esophageal squamous cell carcinoma tie mitochondrial complex I to immunotherapyEMBO Molecular MedicineApril 11, 2026Fahan Ma, Yan Li, Chan Xiang, Bing Wang, Jie Lv, Zhanxian Shang, Weiguang Zhang, Zhaoyu Qin, Yan Pu, Kai Li, et al.single cells were prepared from tumors using Tumor Dissociation Kits (Miltenyi Biotec, catalog No.: 130-095-929) following the manufacturer’s instructions. Digested tumors were filtered through a 70 μm cell strainer

(12) Transcription Factor Subtype Governs Response and Resistance to DLL3-Directed T-Cell Engagement in Small Cell Lung CancerbioRxivApril 9, 2026Damien Vasseur, Shin Saito, Gunsagar S. Gulati, Garyoung Gary Lee, Yasmin N. Laimon, Berkay Simsek, Maddie Lerner, Hyeonseo Cho, Yixiang Li, Tianchu Wang, et al.obtained and dissociated into single cell using the human tumor dissociation kit (Miltenyi Biotech, #130-095-929) within two hours of resection by running on gentleMACS™ Octo Dissociator with the “37C_h_TDK_2” program.

(13) Chemoradiation Reprograms Tumor Cells and the Immune Microenvironment in Cervical CancerCancer ResApril 2, 2026Tito A. Sandoval, Yize Li, Naoshad Muhammad, Tresha Desai, Reyka G. Jayasinghe, Saman Zeeshan, Kay Jayachandran, Matthew J. Inkman, Michael R Waters, Eliwaza Naomi S Msengi, et al.were minced and enzymatically dissociated using the Human Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929) in DMEM. The suspension was transferred to gentleMACS C-tubes (Miltenyi Biotec, 130-093-237) and processed

(14) High-Dimensional Protein Analysis Uncovers Distinct Immunological and Stromal Features in Primary and Metastatic Pancreatic Ductal AdenocarcinomaCancer ResearchApril 2, 2026Emily Greene, Natalie K. Horvat, Deon Bryant. Doxie, Vaunita Cohen. Parihar, Jayden Kim, Cameron J. Herting, Erin E. Grundy, Ayana T. Ruffin, Alyssa M. Krasinskas, Shishir K. Maithel, et al.C-tubes (Miltenyi Biotec, #130-093-237) containing RPMI-1640 and enzymes H, R, and A (Miltenyi Biotec, #130-095-929). Samples underwent two cycles of mechanical dissociation (program h_tumor_01) with the gentleMACS dissociator,

(15) Bridging phenotype and function in bladder cancer using immuno-competent organoids and ex vivo drug screeningJournal of Experimental & Clinical Cancer ResearchApril 1, 2026Carlotta Frascolla, Riccardo Mastroianni, Catarina Macedo-Silva, Marta De Menna, Giulia Orlandi, Daniela Angela Covino, Martina Minoli, Martina Radic, Federico La Manna, Claudio Pulito, et al.were transferred to a T75 flask. Single-cell suspension was obtained using the Tumor Dissociation Kit (130–095–929, Miltenyi Biotec), according to the manufacturer’s protocol. Tissue digestion was performed at 37 °C

(16) Gold Nanoparticles Enhance Radiosensitivity in Glioblastoma CellsACS OmegaMarch 31, 2026Laura Coppola, Giovanna Navarra, Giorgio Avilia, Carlos Cuestas-Ayllón, María Moros, Jesús Martínez de la Fuente, Pasqualino De Antonellis, Maria Teresa Gentile, Laura Mosca, Caterina Oliviero, et al.of tumor tissue using the gentleMACS Dissociator and Tumor Dissociation Kit (Miltenyi Biotec, code # 130-095-929). The cell suspension was filtered, washed with RPMI-1640 (Microtech), and centrifuged before being

(17) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.5 min at 4 °C), resuspended in human tumor dissociation enzyme solution (Miltenyi Biotec, cat. no. 130-095-929) and homogenized using a gentleMACS semi-automated dissociator (Miltenyi Biotec). Homogenized tissue

(18) Ectopic NMDAR expression in cancer unmasks germline-encoded autoimmunityNatureMarch 25, 2026Sam O. Kleeman, Kevin Michalski, Xiang Zhao, Ruben Steigerwald, Miriam Ferrer, Llewelyn Levett, Ethan Ertel, Austin Schultz, Noriko Simorowski, Pamela Moody, et al.were processed into single-cell suspensions using the human Tumour Dissociation Kit (Miltenyi Biotec, 130-095-929) and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s protocol. After red blood cell

(19) Chemoradiation Reprograms Tumor Cells and the Immune Microenvironment in Cervical CancerCancer ResMarch 20, 2026Tito A. Sandoval, Yize Li, Naoshad Muhammad, Tresha Desai, Reyka G. Jayasinghe, Saman Zeeshan, Kay Jayachandran, Matthew J. Inkman, Michael R Waters, Eliwaza Naomi S Msengi, et al.were minced and enzymatically dissociated using the Human Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929) in DMEM. The suspension was transferred to gentleMACS C-tubes (Miltenyi Biotec, 130-093-237) and processed

(20) Tumors Located in the Brain Impair the Frequency and Phenotype of Dendritic Cells in Blood and TumoriScienceMarch 20, 2026Bryan Gardam, Tessa Gargett, Eunwoo Nam, Sidra Khan, Rebecca J. Ormsby, Santosh I. Poonnoose, Julie M. Bracken, Anupama Pasam, Sakthi Lenin, Briony L. Gliddon, et al.,Stemcell Technologies,Cat#07851 DMSO,Sigma-Aldrich,Cat#D2650 Human Tumor Dissociation Kit,Miltenyi Biotec,Cat#130-095-929 IVISbrite D-Luciferin,Revvity,Cat#122799 RPMI 1640 Medium,ThermoFisher Scientific,Cat#11875093 FBS,CellSera,Cat#AU-FBS/PG Myelin

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1/6/2021Human Tumor Dissociation Kit is Adequate for Single Cell Generation Ab GCervical tumor specimens from mice were dissociated with the kit to yield single cells for flow cytometry analysis.

Tumor Dissociation Kit, human (for 25 preparations)

Tumor Dissociation Kit, human from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Tumor Dissociation Kit, human from Miltenyi Biotec

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