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Polymerase Chain Reaction (PCR)
Primers and Probes
Oligo(dT)15 Primer
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Oligo(dT)15 Primer
Oligo(dT)15 Primer
Oligo(dT)15 Primer from Promega
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Citations:
(137)
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Oligo(dT)15 Primer
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Description
Use for first-strand cDNA synthesis with a reverse transcriptase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Oligo(dT)15 Primer
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
C1101
Target
Poly A
Quantity
20microg
Conjugate/Tag/Label
Unconjugated
Primer Type
Oligo dT Primers
Sequence
Oligo(dT)-15
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (137)
(1)
Mechanistic and preclinical evaluation of SIRT3 as a therapeutic target in melanoma
Journal of Dermato-Oncology
December 31, 2026
Karla B. Anaya Aldrete, Mary A. Ndiaye, Glorimar Guzmán-Pérez, Gabriella R. Zaemisch, Chandra K. Singh, Gagan Chhabra, Nihal Ahmad
prepared using MMLV reverse transcriptase (MMLV-RT,
Promega
#
M1705
) and Oligo dT primers (
Promega
#
C1101
) and used for
qPCR
and
PCR
Arrays. Samples were tested for gDNA contamination using
PrimePCR
DNA contamination
(2)
Seedless 3' pairing enables miR-17 family miRNAs to seize let-7 target sites
Nucleic Acids Res
August 14, 2026
Artur Laski, Anneke Brümmer, Matije Lucic, Elisa Federici, Iris Mestres-Pascual, Jochen Imig, Alexander Kanitz, Mihaela Zavolan, Luca F R Gebert, Ian J MacRae,
et al.
(
Thermo Fisher Scientific
, #
4366596
) with a
1:1
mix of random hexamer (
Promega
, #
C1181
) and oligo (dT)15 primers (
Promega
, #
C1101
).
qPCR
was performed using KAPA SYBR
FAST qPCR
master mix (Kapa Biosystems, #KK4618) on a LightCycler
(3)
AP-1B Controls Several Levels of Cell Proliferation and Differentiation in Mouse Enteroids
Biol Cell
August 3, 2026
Maela Duclos, Anne Bourdais, Ophélie Nicolle, Alexandre Helpiquet, Grégoire Michaux, Aurélien Bidaud-Meynard
kit (
Macherey
–Nalgen, 740984). cDNAs were obtained by reverse transcription using oligo(dT) (
Promega
,
C1101
) and
SuperScript III Reverse Transcriptase
(
Thermo Fisher Scientific
,
18080093
) from identical mRNA
(4)
IL-6-induced C/EBPα drives follicular regulatory T cell differentiation to regulate humoral immunity in mice
Nature Communications
July 7, 2026
Haena Lee, Chan Johng Kim, Hyunsoo Ahn, Gayeon Cho, Sang-Hyeon Mun, Seung Won Kim, Hyung-Seok Choi, Dahun Um, Hyeongseop Kim, Haeun Ko,
et al.
Molecular Research Center). cDNA was synthesized from 500 ng of total RNA using oligo(dT) primers (
C1101
,
Promega
) and
ImProm-II Reverse Transcriptase
(
A3802
,
Promega
). cDNA was amplified with SYBR Premix
(5)
Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state
Journal of Virology
June 23, 2026
Maarit Suomalainen, Walther Haenseler, Jonas Kolibius, Andreas Plückthun, Patrick Hearing, Urs F. Greber
(
Thermo Fisher Scientific
,
R0193
), 10 mM
dithiothreitol (DTT
), 25 µg/mL
oligo(dT)15 primer
(
Promega
,
C1101
), 1×
M-MLV reverse transcriptase
(RT) buffer (Promega M531A), 100 units M-MLV RT (
Promega
,
M1701
; no
(6)
Protocol for quantifying silent ribosome induction in yeast and mammalian cells using polysome profiling
STAR Protocols
June 19, 2026
Sayanur Rahaman, Samuel Mondal, Nicole Schiffelholz, Attila Becskei
Scientific,Cat#18090200 Nuclease Free Water,
Thermo Scientific
,Cat#
AM9932
Random hexamers,
Promega
,Cat#
C1181
oligodT15,
Promega
,Cat#
C1101
KAPA SYBR Fast,Sigma Aldrich,Cat#KK4611 Tris-HCl pH 8.0,
Thermo Scientific
,Cat#
AM9856
MgCl2,
Thermo Scientific
(7)
Light-controlled disruption of cancer cell dormancy via photoswitchable stress hormone receptor degraders
Proc Natl Acad Sci U S A
May 26, 2026
Karina M. Freitag, Robin Scheuplein, Chiara Orlacchio, Viola Ansuinelli, Tommaso Fava, Vincent Fischer, Bohan Zhang, Miriam Kretschmer, Mahshid Gazorpak, Erick M. Carreira,
et al.
Complementary DNA (cDNA) using
M-MLV Reverse Transcriptase
(
Promega
,
M1701
), Oligo(dt) 15 primers (
Promega
,
C1101
),
dNTP mix
(
Thermo Scientific
,
R0192
), and
RNasin Ribonuclease Inhibitor
(
Promega
,
N2511
), following
(8)
Mitochondrial copper stabilizes lipoylated TCA cycle proteins to sustain metabolism and proliferation
bioRxiv
May 25, 2026
Santanu Ghosh, Andrew F. Jarvis, Jordi C.J. Hintzen, Nate R. McKnight, Pedro Costa-Pinheiro, Noelle H. Noughton, Yumi Kim, Alison Jaccard, Scot C. Leary, Paul A. Cobine,
et al.
following standard protocols and reverse transcribed to cDNA using using oligo dT primers (
Promega
C1101
). The cDNA was then quantified using the Taqman probe Mm00433246_m1 to detect
mouse
Fdx1
, Mm00513470_m1
(9)
Macrophage-mediated refinement of the dural lymphatic regulates social behavior
Neuron
April 24, 2026
Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu,
et al.
The first reaction consisted of a mix of dNTP (10mM) (
Promega
, cat.
U1515
), Oligo DT (
Promega
, cat.
C1101
), and random primers (
Promega
, cat.
C1181
). The reverse transcription was performed with the
SuperScript™ III Reverse Transcriptase
(10)
SiO 2 ‐CaO CME /Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation
Advanced Healthcare Materials
February 2, 2026
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin
Primer annealing: 0.125 µg of total RNA was mixed with 1 µl of 20 µg ml−1 Oligo(dT) primers (
Promega
,
C1101
) and RNase‐free water to a final volume of 35 µl. The mixture was incubated at 65°C for 5 min; (2)
cDNA synthesis
(11)
Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcoma
EMBO Molecular Medicine
January 3, 2026
Veveeyan Suresh, Christoph Hafemeister, Andri Konstantinou, Sarah Grissenberger, Caterina Sturtzel, Martha M Zylka, Florencia Cidre-Aranaz, Andrea Wenninger-Weinzierl, Karla Queiroz, Dorota Kurek,
et al.
µg of RNA,
M-MLV Reverse Transcriptase
(
Promega
, Cat#
M1705
), and Oligo(dT)15 primers (
Promega
, Cat#
C1101
).
PCR amplification
was performed using the resulting cDNA as a template, knock-in validation primers
(12)
Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins
The Journal of physiology
October 1, 2025
Afuwape OAT, Chanaday NL, Kasap M, Monteggia LM, Kavalali ET.
080‐093). Following its
first‐strand cDNA synthesis
protocol, we used
Promega
's Oligo(dT)15 (Cat. #
C1101
) primer and
Recombinant RNasin Ribonuclease Inhibitor
(
N2511
). cDNA concentration and quality were confirmed
(13)
ADSL deficiency is a secondary mitochondrial disease affecting organelle homeostasis and ERK2/AKT signaling in a linear genotype-phenotype relation
Cell Reports
September 23, 2025
Matteo Bordi, Beatrice Testa, Claudia Compagnucci, Fiorella Colasuonno, Francesca Cipressa, Elisabetta Betterini, Andrea Mancini, Claudia Carsetti, Illari Salvatori, Caterina Ferraina,
et al.
manufacturer’s instructions, and 2 μg RNA were retrotranscribed using M-MLV enzyme and oligodT (
Promega
#
C1101
).
Real-Time PCR
was performed using SYBR Green (
SYBR Green PCR Master Mix
;
Thermo Fisher Scientific
(14)
A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity
Sci Adv
February 28, 2025
Liam Pollock, Ioanna Ch. Georgiou, Emma V. Rusilowicz-Jones, Michael J. Clague, Sylvie Urbé
polymerase chain reaction (PCR
) nucleotide mix (Promega; U144B), and
oligo (dT) 15 Primer
(
Promega
;
C1101
).
Quantitative PCR
were performed in triplicate using primers against
PINK1
(5’-GGACGCTGTTCCTCGTTA-3′,
(15)
IFN-γ licenses normal and pathogenic ALPK1/TIFA pathway in human monocytes
iScience
January 17, 2025
Amandine Martin, Solène Caron, Mélissa Marcotte, Pauline Bronnec, Etienne Garneret, Nora Martel, Georgina Maalouf, Pascal Sève, David Saadoun, Yvan Jamilloux,
et al.
cartridges 12–230 kDa",Bio-techne,Cat#SM-W004
Random primers
,
Promega
,Cat#
C1181
OligodT primers,
Promega
,Cat#
C1101
FuGENE® HD Transfection Reagent
,
Promega
,
E2311
; CAS: 64-17-5
TRI Reagent
,
Sigma-Aldrich
,Cat#
T9424
7-AAD Viability Staining Solution
(16)
Loss of Kat2b impairs intraflagellar transport and the Hedgehog signaling pathway in primary cilia
Sci Rep
January 16, 2025
Jae Hee Jun, Hwayeon Cha, Je Yeong Ko, Ho-Shik Kim, Kyung Hyun Yoo, Jong Hoon Park
M-MLV Reverse Transcriptase
(M170B;
Promega
),
RNasin® ribonuclease inhibitor
(N211A; Promega), 100 nM oligo-dT (
C1101
;
Promega
), and 2.5 nM dNTP mixture (
U1205
,
U1225
,
U1215
, and
U1235
;
Promega
).
qRT-PCR
was performed
(17)
The endonuclease activity of MCPIP1 controls the neoplastic transformation of epithelial cells via the c-Met/CD44 axis
Cell Commun Signal
January 15, 2025
Paulina Marona, Rafał Myrczek, Iga Piasecka, Judyta Gorka, Oliwia Kwapisz, Ewelina Pospiech, Janusz Rys, Jolanta Jura, Katarzyna Miekus
performed using 1 mg of total RNA,
oligo(dT) 15 primer
(1 μg/μl, Promega, Madison, WI, USA, cat. no.
C1101
), dNTPs (10 mM,
Promega
, cat. no.
U1330
) and
M-MLV reverse transcriptase
(
Promega
, cat. no.
M1701
).
(18)
Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro
Sci Rep
January 2, 2025
Amaral Ana, Sadowska Agnieszka, Cerveira-Pinto Marta, Kordowitzki Pawel, Skarzynski Dariusz, Ferreira-Dias Graça, Anna Szóstek-Mioduchowska
(
M5313
;
Promega
; Madison, USA) from 1000 ng total RNA in a 20 µL reaction volume using oligo(dT) primer (
C1101
;
Promega
). The
qPCR
reactions were run in
StepOnePlus™ Real-Time PCR System
(
Applied Biosystems
, Warrington,
(19)
GDF15 controls primary cilia morphology and function thereby affecting progenitor proliferation
Life Science Alliance
July 1, 2024
Katja Baur, Şeydanur Şan, Gabriele Hölzl-Wenig, Claudia Mandl, Andrea Hellwig, Francesca Ciccolini
retrotranscribed into cDNA using
M-MLV reverse transcriptase
(#
M1701
;
Promega
) and Oligo(dT)15 primers (#
C1101
;
Promega
) according to the manufacturer’s instructions in a ProFlex
PCR
machine (Applied Biosystems). 1
(20)
Sperm chromatin accessibility's involvement in the intergenerational effects of stress hormone receptor activation
Translational psychiatry
December 8, 2023
Fischer V, Kretschmer M, Germain PL, Kaur J, Mompart-Barrenechea S, Pelczar P, Schürmann D, Schär P, Gapp K.
M-MLV Reverse Transcriptase
(
Promega
, Walldorf, Germany,
M1705
) and Oligo(dt) 15 primers (
Promega
,
C1101
) containing
RNasin® Plus RNase inhibitor
(
Promega
,
N2615
) according to the manufacturer’s recommendations.
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Oligo(dT)15 Primer from Promega
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