NspI from New England Biolabs

Product Specs
ItemNspI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0602S
Quantity250 units
ApplicationsDNA Manipulation
Concentration5 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

(AG)CATG/(CT)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Ligament injury in adult zebrafish triggers ECM remodeling and cell dedifferentiation for scar-free regenerationNPJ Regenerative medicineSeptember 19, 2023Anderson T, Mo J, Gagarin E, Sherwood D, Blumenkrantz M, Mao E, Leon G, Levitz H, Chen HJ, Tseng KC, et al.GGTGAGAAAATGAAACCCGAAACTAGTCT). The 500 bp PCR product was digested with NspI (New England Biolabs, R0602L) that recognizes a RCATG/Y cut site only present in mutants. Mutant alleles were then identified by

(2) Znf469 Plays a Critical Role in Regulating Synthesis of ECM: A Zebrafish Model of Brittle Cornea SyndromeInvestigative Opthalmology & Visual ScienceMay 31, 2023Jing Bao, Xiaoning Yu, Xiyuan Ping, Xingchao Shentu, Jian Zouof NspI. The 4-bp deletion strain was genotyped by PCR and digestion with restriction enzyme NspI (R0602S; New England Biolabs, Ipswich, MA, USA) (Fig. 2E). The genotyping PCR primers were as follows: F: 5′-CAAACCAACGCTCAAATCCCAC-3′R:

(3) Elevated enhancer-oncogene contacts and higher oncogene expression levels by recurrent CTCF inactivating mutations in acute T cell leukemiaCell ReportsApril 14, 2023Willem K. Smits, Carlo Vermeulen, Rico Hagelaar, Shunsuke Kimura, Eric M. Vroegindeweij, Jessica G.C.A.M. Buijs-Gladdines, Ellen van de Geer, Marjon J.A.M. Verstegen, Erik Splinter, Simon V. van Reijmersdal, et al.,New England Biolabs,Cat# R0125S T4 DNA ligase,New England Biolabs,Cat# M0202S NspI,New England Biolabs,Cat# R0602S TRIzol Reagent,Thermo Fisher Technology,Cat# 15596026 Acid-Phenol:Chloroform pH4.5,Thermo Fisher Technology,Cat#

(4) Ligament injury in adult zebrafish triggers ECM remodeling and cell dedifferentiation for scar-free regenerationbioRxivFebruary 8, 2023Troy Anderson, Julia Mo, Ernesto Gagarin, Desmarie Sherwood, Maria Blumenkrantz, Eric Mao, Gianna Leon, Hung-Jhen Chen, Kuo-Chang Tseng, Peter Fabian, et al.GGTGAGAAAATGAAACCCGAAACTAGTCT). The 500bp PCR product was digested with NspI (New England Biolabs, R0602L) that recognizes a RCATG/Y cut site only present in mutants. Mutant alleles were then identified by

(5) tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous lociNucleic Acids ResearchDecember 1, 2017Alina Ott, Sanzhen Liu, James C. Schnable, Cheng-Ting ‘Eddy’ Yeh, Kai-Sin Wang, Patrick S. SchnableDNA from each sample was digested with 100 units of NspI [New England Biolabs (Beverly, MA, USA), No. R0602L] and 400 units of BfuCI [New England Biolabs (Beverly, MA, USA), No. R0636L] in NEB CutSmart Buffer

(6) The reverse evolution from multicellularity to unicellularity during carcinogenesisNature CommunicationsMay 5, 2015Han Chen, Fangqin Lin, Ke Xing, Xionglei HeDNA was extracted from B5 106 cells using the DNeasy Kit (Qiagen 69504) and digested by NspI (NEB R0602L) or StyI (NEB R0500L). Adaptors were ligated to the DNA fragments for PCR ampliﬁcation. The ampliﬁed

(7) Integrative Oncogenomic Analysis of Microarray Data in Hematologic MalignanciesMethods in Molecular BiologyNovember 3, 2009Jose A Martínez-Climent, Lorena Fontan, Vicente Fresquet, Eloy Robles, María Ortiz, Angel Rubio(10,000 U/mL): New England Biolabs (NEB), P/N R0500S • NspI (10,000 U/mL): New England Biolabs (NEB), P/N R0602L • AccuGENE® Water, Molecular Biology-Grade, Cambrex, P/N 51200 • T4 DNA Ligase: New England Biolabs

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NspI

NspI from New England Biolabs

Sequence

New England Biolabs

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NspI from New England Biolabs

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