NotI from Promega

Product Specs
ItemNotI
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberR6431
Quantity200u
Concentration10u/µl

Sequence

GC▼GGCC GC
CG CCGG▲CG

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Geosmin, a Food- and Water-Deteriorating Sesquiterpenoid and Ambivalent Semiochemical, Activates Evolutionary Conserved Receptor OR11A1Journal of Agricultural and Food ChemistryJuly 17, 2024Lena Ball, Tim Frey, Franziska Haag, Stephanie Frank, Sandra Hoffmann, Matthias Laska, Martin Steinhaus, Klaus Neuhaus, Dietmar Krautwurstpolymerase chain reaction (PCR) using gene-specific primers (Table S2). Amplicons were EcoRI/NotI-digested (#R6017/#R6435; Promega, Madison, USA), ligated with T4-DNA ligase (#M1804; Promega, Madison, USA) into the expression

(2) Petrol Note in Riesling – 1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) Selectively Activates Human Odorant Receptor OR8H1Journal of Agricultural and Food ChemistryMarch 6, 2024Franziska Haag, Tim Frey, Lena Ball, Sandra Hoffmann, Dietmar Krautwurstprimers (Table S2), ligated with T4-DNA ligase (#M1804, Promega, Madison, USA) EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) into the expression plasmid (#pFN210A SS-HaloTag CMV-neo Flexi-Vector, Promega,

(3) Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1Journal of Chemical Information and ModelingApril 10, 2023Alessandro Nicoli, Franziska Haag, Patrick Marcinek, Ruiming He, Johanna Kreißl, Jörg Stein, Alessandro Marchetto, Andreas Dunkel, Thomas Hofmann, Dietmar Krautwurst, et al.expression vectors was done with T4-DNA ligase (#M1804, Promega, Madison, USA) via EcoRI/NotI (#R6017/#R6435, Promega, Madison, USA) into the expression plasmid pFN210A100 and verified by Sanger sequencing using

(4) The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogasteriScienceNovember 18, 2022Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik HedenstrÃ¶m, BjÃ¶rn Bohman, Marie Bengtsson, Paul G. Becher, et al.USA) either MfeI/NotI (#R3589S/ # R0189S, New England Biolabs, Ipswich, UK) or EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) into the expression plasmid (#pFN210A SS-HaloTag® CMV-neo Flexi®-Vector, Promega,

(5) An evolutionary conserved olfactory receptor for foodborne and semiochemical alkylpyrazinesThe FASEB JournalJune 1, 2021Patrick Marcinek, Franziska Haag, Christiane Geithe, Dietmar KrautwurstMfeI/NotI‐digested (#R0589S/#R0189S, New England BioLabs, Ipswich, USA) or EcoRI/NotI‐digested (#R6017/#R6435, Promega, Madison, USA), ligated into expression plasmid IL6‐HaloTag‐pFN210A 35 using T4‐DNA ligase

(6) miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature StressFrontiers in Cell and Developmental BiologyApril 9, 2021QingJian Liang, WenNa Dong, MuFei Ou, ZhongHua Li, Can Liu, FeiFei Wang, Yuan Liu, WeiNa Wang

(7) Copper-mediated thiol potentiation and mutagenesis-guided modeling suggest a highly conserved copper-binding motif in human OR2M3Cellular and Molecular Life SciencesJune 1, 2020Franziska Haag, Lucky Ahmed, Krystle Reiss, Eric Block, Victor S. Batista, Dietmar KrautwurstOR2M3 and OR1A1 (#R0589S/#R0189S, New England BioLabs, Ipswich, USA) or EcoRI/NotI for OR2W1 (#R6017/#R6435, Promega, Madison, USA) into the expression plasmid pI2-dk(39aa-rho-tag) (aa, amino acid) [53, 54],

(8) An intestinal zinc sensor regulates food intake and developmental growthNatureApril 1, 2020Redhai S, Pilgrim C, Gaspar P, Giesen LV, Lopes T, Riabinina O, Grenier T, Milona A, Chanana B, Swadling JB, et al.primers Hodor F and Hodor R (see below). The PCR product was digested with NotI and EcoRI (Promega #R6435 and #R6017 respectively) and cloned into the pUASTattB vector53. hodor-containing pUASTattB was amplified,

(9) Notch Signaling Regulates MMP-13 Expression via Runx2 in ChondrocytesScientific reportsOctober 30, 2019Xiao D, Bi R, Liu X, Mei J, Jiang N, Zhu S.TaKaRa, Japan). cDNA was confirmed by DNA sequencing. After digestion with BglII and NotI (R6081 & R6431, Promega, USA), purification from agarose gel, PCR products was subcloned into pCMV-myc-C vector (635689,

(10) IL-6-HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptorsJournal of Biological MethodsNovember 3, 2017Franziska Noe, Tim Frey, Julia Fiedler, Christiane Geithe, Bettina Nowak, Dietmar Krautwurstpurified (gel extraction kit, #28706, Qiagen, Hilden, Germany), digested either EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) or MfeI/NotI (#R0589S/ #R0189S, New England Biolabs, Ipswich, USA) and ligated

(11) Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobiaBMC Plant BiologyDecember 1, 2014Araceli Nora García, Nicolás Daniel Ayub, Ana Romina Fox, María Cristina Gómez, María José Diéguez, Elba María Pagano, Carolina Andrea Berini, Jorge Prometeo Muschietti, Gabriela SotopCR2.1TOPO vector (Cat. # K4500-01, Invitrogen). The pTOPO-MsSN1 plasmid was digested with Not I (Cat. # R6431, Promega), treated with Klenow, and religated to destroy the polylinker Not I site. The resulting plasmid

(12) The inhibition of lung cancer cell growth by intracellular immunization with LC-1 ScFvCell ResearchApril 11, 2002Liang CHEN, Gang LI, Lei TANG, Jue WANG, Xi Rui GEmodified and amplified LC-1 ScFv was digested with Sfi I (Cat No. R6391, Promega Co.) and Not I (Cat No. R6431, Promega co.), and subcloned into phagemid pCantab5E (Cat No. 27-9401-01, Amersham Pharmacia Biotech Co.).

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NotI

Description

Sequence

Promega

Citations (12)

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NotI from Promega

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