NotI from New England Biolabs

Product Specs
ItemNotI
CompanyNew England Biolabs
Price $329.00 Inquire
Catalog NumberR0189L
Quantity2500 units
Concentration10-50 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC/GGCCGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Structural and functional characterization of a conserved cryptic epitope on SARS-CoV-2 spike S2 subunitPLoS PathogAugust 3, 2026Ling Zhou, Ching-Lin Hsieh, Sarah R. Leist, Emily Happy Miller, Andrew P. Horton, Sophie R. Shoemaker, Elizabeth C. Gardner, John M. Powers, Daniel R. Boutz, Michael L. Mallory, et al.recombination at the Leu2 locus. For each 1 μg library plasmid, 0.5 μL NotI (10 units/μL) (NEB, Cat. No. R0189) was used in 10 μL reactions with the supplied Buffer 3.1. Reactions were incubated overnight at 37

(2) Ketamine persists within neuronal compartments long after systemic clearanceResearchSquareJuly 22, 2026Joseph Cichon, Kallol Bera, Zachary Blumenfeld, Elaine Lin, Meah Ahmed, Eve Fine, Md. Nadim Hossain, Andrey Andreev, Altyn Rymbek, Peter Fenton, et al.enzyme EcoRI,NEB,R0101S,Restriction enzyme HindIII,NEB,R0104S,Restriction enzyme SalI,NEB,R0138S,Restriction enzyme NotI,NEB,R0189S,Restriction enzyme BglII,NEB,R0144S,Restriction enzyme Agel,NEB,R3552S,Restriction enzyme EcoRV,NEB,R0195S,Restriction

(3) Autosomal recessive A20 zinc finger 7 mutation is associated with early-onset lupus-like diseaseInflamm ResJuly 18, 2026Mohamed Alsabbagh, Satanay Z. Hubrack, Lara Gamgoum, Maryiam J. A. Osman, Katherine E. Ford, Alya Al Shakaki, Amal Robay, Housam Sarakbi, Samar Al Emadi, Rafah Mackeh, et al.RC221337) using the restriction enzymes EcoRI (New England Biolabs #R0101) and NotI (New England Biolabs #R0189) and ligating it into pcDNA3-mRFP, which was a gift from Doug Golenbock (Addgene plasmid #13032; http://n2t.net/addgene:13032;

(4) The maternal KRAB-ZFP ZFPOBI1 reveals structural constraints governing ERV transcriptional co-option in mouse oocytesFront Cell Dev BiolJuly 14, 2026Andrina J. Stäubli, Yan Huang, Louise J. H. Rasmussen, Freja Mosegaard Hansen, Hui Mun Loh, Michael J. Claxton, Daniel M. Messerschmidt(DNA2.0) previously modified with chicken β-actin promoter and N-terminal triple HA tag using NotI (NEB, R0189S) restriction sites (Seah et al., 2019). For luciferase reporter assays respective RLTR10 elements were

(5) MYC serine 62 phosphorylation promotes its association with DNA double-strand breaks to facilitate repair and cell survival under genotoxic stressGenes & DevelopmentJuly 1, 2026Gabriel M. Cohn, Colin J. Daniel, Jennifer R. Eng, Alannah S. MacDonald, Jonathan St-Germain, Nadja M. Pieper, Toshita Kannan, Xiao-Xin Sun, Carl Pelz, Ariffin Ali, et al.(Addgene 16011) and inserted into pcDNA4/TO (Invitrogen V102020) via HindIII (NEB R0104T) and NotI (NEB R0189L). The HA-BioID2 only control sequence (Invitrogen 74224) was cloned into pcDNA4/TO via HindIII and BspEI

(6) Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulationNucleic Acids ResearchJune 30, 2026Junichiro Ohzeki, Akiko Watanabe, Jia Xian Lee, Chiharu Minami, Kazuto Kugou, Kyotaro Yamazaki, Kenta Shirasawa, Sachiko Isobe, Yusuke Endo, Yasuhiro Kazukidigested with BamHI (1010A, TAKARA)-SpeI (R3133L, NEB) (odd number unit), NheI (R3131L, NEB)-NotI (R0189L, NEB) (even number unit), or BamHI-NotI (pNX31C vector DNA), respectively. These odd-numbered and even-numbered

(7) Protocol for streamlining genotyping of germline-transmissible mutants from genome editing by using a parallel qPCR-based index and R analysisSTAR ProtocolsJune 19, 2026Emeric M. Louis, Liezhen Fu, Nga Luu, Laurent M. Sachs, Yun-Bo Shipolymerase-based PCR amplification system,Promega,M7502 NotI restriction enzyme,New England Biolabs,R0189S 1× NEBuffer r3.1,, Human chorionic gonadotropin (hCG),Pregnyl,78206-150-01 UltraPure™ DNase/RNase-free distilled water

(8) TRK-T3 condensate organization drives growth signalingLife Sci AllianceJune 17, 2026Sindy R Chavez, Savannah M Bogus, Samantha N Cheung, Winnie Zhou, Lilja Gudmundsson, Kaho Kato, Andreas M ErnstpmEGFP-FLAG and pmCherry-FLAG previously described (Bogus et al, 2025) were both digested with NheI (R0189S; NEB) and AgeI (R3552S; NEB) restriction enzymes to insert the commercially obtained gene via ligation

(9) Synthetic rewriting of the IGH locusCell GenomicsJune 10, 2026Zong-Heng Fu, Yuan Ma, Si Cheng, Yuan Zhong, Nan Wang, Zhaoqing Ba, Guang-Rong Zhao, Yi Wualkaline lysis from small-scale (5–10 mL) saturated bacterial cultures with NotI-HF (New England Biolabs, R0189L). The digestion reactions were conducted at 37°C for 1–2 h. The entire reaction mixture was then separated

(10) Microtubule-templated actin assembly by septin9 drives apical expansion of epithelial cellsbioRxivMay 23, 2026Angelo Arrigo, Victoria Hua, Elias T. Spiliotis, Saurabh Kulkarni,New England Biolabs,E2611S NEBuilder HiFi DNA Assembly Cloning Kit,New England Biolabs,E5520S NotI,New England Biolabs,R0189S KpnI-HF,New England Biolabs,R3142S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S mMESSAGE mMACHINE™ SP6 Transcription Kit

(11) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapatstrategy. For the full-length and Δ constructs (Δ1 and Δ2) in pUAST, KpnI-HF (NEB, #R3142S) and NotI (NEB, #R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S);

(12) Gypenosides inhibit melanoma proliferation, migration and enhance the anti-tumor immunity of CD8+ T cells via ETV5/PD-L1 signalingJournal of Translational MedicineApril 22, 2026Yue Yin, Wenjing Lai, Changpeng Hu, Huyue Zhou, Yali Liu, Yafeng Liu, Min Yang, Kan He, Guobing Liwas linearized through double digestion using BamH1 (#R0136L, New England Biolabs, USA) and NotI (#R0189L, New England Biolabs, USA) restriction enzymes under standard reaction conditions. Following purification

(13) Mechanistic dissection of BLTP2 targeting to ER-PM contact sitesbioRxivApril 17, 2026Sarah D. Neuman, Amy T. Cavanagh, Nicole Sheffels, Elizabeth Conibear, Arash BashirullahBashirullah, 2018) was digested with SpeI-HF (New England Biolabs R3133) and NotI (New England Biolabs R0189), and the resulting 290 bp fragment was cloned into pGEM T-easy (Promega A1360). The deletions were

(14) Primary ciliogenesis is promoted during epithelial-mesenchymal transition via a miR-200 – DZIP1 axisCell Communication and SignalingApril 1, 2026Laura Sourdin, Julie M. Bracken, Katherine A. Pillman, Andrew G. Bert, Yeesim Khew Goodall, Philip A. Gregory, Gregory J. Goodall, Cameron P. BrackenBioline, #Bio-52060), digested with XbaI (New England Biolabs, #R0145) and NotI (New England Biolabs, #R0189) and ligated with T4 DNA Ligase (New England Biolabs, #M0202). Ligations were transformed into Alpha-Select

(15) A genome-scale single-cell CRISPRi map of trans gene regulation across human pluripotent stem cell linesCell GenomicsFebruary 11, 2026Claudia Feng, Elin Madli Peets, Yan Zhou, Luca Crepaldi, Sunay Usluer, Alistair Dunham, Jana M. Braunger, Jing Su, Magdalena E. Strauss, Daniele Muraro, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, BbsI,NEB,R0539L NotI,NEB,R0189S EcoRV-HF,NEB,R3195S ,, Critical commercial assays,Critical commercial assays,Critical commercial

(16) STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondriaThe EMBO JournalFebruary 11, 2026Yolanda Orantos-Aguilera, Irene Sanchez-Lopez, Carlos Pascual-Caro, Patricia Gómez-Suaga, Estela Area-Gomez, Eulalia Pozo-Guisado, Jorge Montesinos, Francisco Javier Martin-Romerokit,BioTools,4542 Nitrocellulose Blotting Membrane,AmershamTM ProtranTM,10600001 NotI,New England Biolabs,R0189 NuPAGE-LDS sample buffer,Invitrogen,NP0007 PBS,Fisher BioReagents,BP3994 Percoll®,GE Healthcare,17-0891-02 Phenylmethylsulphonyl

(17) Modular gene tagging in C. elegansResearch SquareFebruary 10, 2026Erik Jorgensen, Kevin Hefel, Kevin Kruse, Soren B. Jorgensen, Kam Hoe Jorgensen, Ryan Stolley, Matthew S. Stolley, Matthew Jorgensento generate pAJH103, after which we cut and added SapI sites by restriction digest with NotI (NEB® R0189) and annealed overhanging oligos (pAJH110). Finally Golden Gate assembly was used to assemble Pmex-5::B3::let-858

(18) Long-read sequencing reveals increased isoform diversity in key transcription factor effectors of intercellular signalling at the invertebrate-vertebrate transitionBMC BiologyJanuary 24, 2026Nuria P. Torres-Aguila, Marika Salonna, Sebastian M. Shimeld, Stefan Hoppler, David E. K. Ferrierresistance and their composition was confirmed by enzyme digestion with NotI (New England Biolabs, R0189) and Sanger sequencing (Oxford Zoology service, Eurofins service). The sequences were compared against

(19) Modular gene tagging in C. elegansbioRxivJanuary 18, 2026Adam Hefel, Kevin Kruse, Kaden Wall, Soren B. Jorgensen, Kam Hoe Ng, Ryan Stolley, Matthew S. Rich, Erik M. Jorgensento generate pAJH103, after which we cut and added SapI sites by restriction digest with NotI (NEB® R0189) and annealed overhanging oligos (pAJH110). Finally Golden Gate assembly was used to assemble Pmex-5::B3::let-858

(20) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. CravattSupplementary Table 3). The PCR product was cloned into a pET21a vector using NdeI (NEB, R0111S) and NotI (NEB, R0189S) restriction and Gibson assembly (NEB, E2621S). His–METTL5_Gibson_FWD: taactttaagaaggagatatacATATGCACCACCACCACCACCACGAAAATTTGTATTTCCAGTCAATGAAGAAGGTACGTCTG His–METTL5_Gibson_REV:

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Paired- End diTagging for Transcriptome and Genome Analysis
July 01, 2007

NotI

NotI from New England Biolabs

Sequence

New England Biolabs

Citations (210)

Reviews

Protocols

Paired- End diTagging for Transcriptome and Genome Analysis

NotI from New England Biolabs

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