NlaIII from New England Biolabs

Product Specs
ItemNlaIII
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0125S
Concentration10 u/µL
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-80 C

Sequence

CATG/

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Chromatin-intrinsic mechanisms determine orientation-specific class switch recombinationNature CommunicationsFebruary 28, 2026Sha Luo, Ruolin Qiao, Hailiang Zha, Leyi Yu, Xiaoling Shan, Shuchan Li, Zifan Yang, Aoyu Ma, Yaxin Cheng, Yao-Feng Zhao, et al.crosslinked cells were lysed in the 3C lysis buffer, and nuclei were digested with NlaIII enzyme (NEB, R0125) at 37 °C overnight. The digested samples were ligated by T4 DNA ligase (NEB, M1801) at room temperature

(2) CFP1-mediated H3K4me3 broad domains control early B cell lineage fate determinationNucleic Acids ResearchFebruary 23, 2026Wanyu Bai, Yalin Yang, Yanan Zhao, Shuoxu Gong, Yimiao Lu, Zhonghui Xue, Lie Wang, Fei-Long Meng, Chun Chen, Junchao Dong1× protease inhibitors), and nuclei were digested with 9 μl of NlaIII enzyme (New England Biolabs, R0125L) in 191 μl of Digestion Mix buffer (1× CutSmart Buffer, 0.29% SDS, 1.68% Triton X-100) for 2 h at 37°C.

(3) Trimodal single-cell profiling of transcriptome, epigenome, and 3D genome in complex tissues with scHiCARNature BiotechnologyFebruary 19, 2026Xiaolin Wei, Yueyuan Xu, Dongchan Yang, Kyukwang Kim, Lizhi Yi, Wenzhe Luo, Xin Lin, Yu Xiang, Ashley B. Williams, Xiaotao Wang, et al.NlaIII digestion mixture (4.1 μl of ultrapure water, 5 μl 10X Cutsmart buffer, 0.9 μl of NlaIII (NEB, R0125L) at 37 °C for 1 hour. Subsequently, the DNA was purified using 1.6X volume SPRI beads and dissolved

(4) Global genetic rewiring during compensatory evolution in the yeast polarity networkEMBO ReportsFebruary 16, 2026Enzo Kingma, Marieke Glazenburg, Karel Olavarria, Liedewij Laan"Sodium Acetate, 3 M, pH 5.2, Molecular Biology Grade",Merck,567422 RNAse A,QIAGEN,19101 DpnII,NEB,R0543L NlaIII,NEB,R0125L T4 DNA Ligase (5 U/μL),Thermo Fisher Scientific,EL0011 Software,Software,Software Transposonmapper,PyPI,v1.1.4 Cytoscape,cytoscape.org,Version

(5) Effects of topological domain disruption on transcriptional regulation are chromatin context dependentEpigenetics & ChromatinFebruary 1, 2026Ángel Josué Cerecedo-Castillo, Diana Itzé Mojica-Santamaría, Hober Nelson Núñez-Martínez, Carlos Alberto Peralta-Alvarez, Gustavo Tapia-Urzúa, Georgina Guerrero, Rodrigo Gacel Arzate-Mejía, Félix Recillas-Targaand ethanol precipitated, and the second digestion was performed overnight with 50 U of NlaIII (NEB R0125S) or HindIII (NEB R0104S). The second ligation was performed in 5 mL total with 3000 U of T4 DNA ligase

(6) CTCF couples long-range loop extrusion and diffusion to mediate a diverse Igκ repertoireNature CommunicationsDecember 11, 2025Emma L. Bush, Brigette Berke-Reynolds, Kaitlyn M. Hutchins, Xinrui Yu, Jorge A. Colón-Rosado, Fujung Chang, John Curran, Jiaxin Yang, Liangliang Sun, Jianrong Wang, et al.and 1.8% Triton X-100 (37 °C, 1 h each), digested overnight with NlaIII (700 U, New England Biolabs, R0125L), heat-inactivated at 65 °C for 20 min, and ligated under dilute conditions with T4 DNA ligase (100

(7) Profile of GAS6 gene single nucleotide polymorphisms [GAS6 c.834+7G>A (rs8191974), and GAS6 c.1332C>T (rs1803628)] among a cohort of Egyptian women with preeclampsia: Relation to disease risk and severityJournal of Genetic Engineering and BiotechnologyDecember 1, 2025Mostafa Mohamed Khodry, Sawsan M.A. Abuhamdah, Mohammed H. Hassan, Abd El-Naser Abd El-Gaber Ali, Asmaa Ahmed Sayed Khames, Mohammad A.M. Ahmedsupplied by New England Biolabs) was used for the detection of GAS6 c.834 + 7G > A, and NlaIII (catalog No.R0125S, supplied by New England Biolabs) was used for the detection of GAS6 c.1332C > T. One μl of the enzyme,

(8) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic lociCell GenomicsNovember 12, 2025Ke Zhao, Yao Zhou, Xueqi Wang, Zhao Wang, Xinran Xu, Yichen Chen, Lin Zhao, Chengyue Wu, Jianhua Wang, Hongcheng Yao, et al.T4 DNA Ligase,New England Biolabs,Cat#M0202L CviQI,New England Biolabs,Cat#R0639L NlaIII,New England Biolabs,Cat# R0125S EcoRI,New England Biolabs,Cat# R0101S T4 Polynucleotide Kinase (PNK),New England Biolabs,Cat#M0201L Hoechst 33342

(9) Aurora A regulates the material property of spindle poles to orchestrate nuclear organization at mitotic exitThe EMBO JournalSeptember 12, 2025Ashwathi Rajeevan, Vignesh Olakkal, Madhumitha Balakrishnan, Dwaipayan Chakrabarty, François Charon, Daan Noordermeer, Sachin Kotak106404 Nitrocellulose membrane,Bio-Rad,Cat# 1620112 NlaIII restriction enzyme,New England Biolabs,Cat# R0125 Nocodazole,Sigma-Aldrich,Cat# M1404 NP-40 (Tergitol),Sigma-Aldrich,Cat# NP40S PMSF,Sigma-Aldrich,Cat#

(10) DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insightsGenome BiologySeptember 5, 2025Xiaona Chen, Feng Yang, Suyang Zhang, Xiaofan Guo, Jieyu Zhao, Yulong Qiao, Liangqiang He, Yang Li, Qin Zhou, Michael Tim-Yun Ong, et al.samples was then mixed with 50 μl rCutSmart (NEB, B6004), 295 μl H2O, and 50 units of Nla III (NEB, R0125). The mixture was incubated at 37 °C with shaking at 500 rpm on the ThermoMixer overnight. The Nla III

(11) Xist upstream deletion leads to dysregulation of Xist and autosomal gene expressionGenome ResearchSeptember 2, 2025Sudeshna Majumdar, Lakshmi Sowjanya Bammidi, Hemant C. Naik, Avinchal Manhas, Runumi Baro, Akash Kalita, Amlan Jyoti Naskar, Sundarraj Nidharshan, Girija S. Bariha, Dimple Notani, et al.TritonX-100, following which the chromatin was digested using 100 units of the 1st cutter NlaIII (NEB, #R0125L) overnight at 37°C. The digested chromatin was then subjected to ligation using T4 DNA ligase (Invitrogen)

(12) Single-stranded DNA-binding proteins are essential components of the architectural LDB1 protein complexMolecular CellAugust 21, 2025Xiaokang Wang, Nicholas G. Aboreden, Ying Cai, Jessica C. Lam, Kate A. Henderson, Jiaqi Xiang, Belinda M. Giardine, Ross C. Hardison, Cheryl A. Keller, Lalitha Nagarajan, et al.million cells were fixed and processed as previously described91. DpnII (NEB, R0543L) and NlaIII (NEB, R0125L) were used as first and second restriction enzyme, respectively. The following 4C primers were used

(13) SUMO operates from a unique long tandem repeat to keep innate immunity in checkNucleic Acids ResearchJuly 31, 2025Amandine Goffeney, Ivo A Hendriks, Victoria Morel, Yann Loe-Mie, François Charon, Michael L Nielsen, Jack-Christophe Cossec, Daan Noordermeer, Jacob-Sebastian Seeler, Anne Dejeanusing MboI (New England Biolabs, R0147) as primary restriction enzyme and NlaIII (New England Biolabs, R0125) as secondary enzyme. 4C-seq sequencing libraries were produced using a two-step PCR amplification protocol

(14) Nucleosome placement and polymer mechanics explain genomic contacts on 100 kb scalesNucleic Acids ResJuly 24, 2025John Corrette, Jiachun Li, Hanjuan Shao, Praveen Krishna Veerasubramanian, Andrew Spakowitz, Timothy L Downing, Jun Allard65°C. DNA was purified using SPRI beads and subjected to a second RE digestion using NlaIII (NEB, Cat. R0125L) to generate sticky ends for circularization. The digested DNA was then ligated again, and the resulting

(15) Klf5-adjacent super-enhancer functions as a 3D genome structure-dependent transcriptional driver to safeguard ESC identityNat CommunJuly 1, 2025Guangsong Su, Bohan Chen, Yingjie Song, Qingqing Yin, Wenbin Wang, Xueyuan Zhao, Sibo Fan, Jie Lian, Dongqing Li, Jinfang Bi, et al.then DNA was ethanol-precipitated. The purified product was further digested with NlaIII (NEB, ref. R0125L) and then cyclized with T4 ligase. After purification, PCR reactions (TransStart FastPfu DNA Polymerase)

(16) Perinatal death in pig models of hypertrophic cardiomyopathy carrying sarcomere pathogenic variantsJournal of Molecular and Cellular Cardiology PlusJune 1, 2025Tatiana Flisikowska, Björn Petersen, Giulia Mearini, Daniela Huber, Mayuko Kurome, Melanie Stoff, Saskia Schlossarek, Andrea Lucas-Hahn, Eckhard Wolf, Judith Montag, et al.allele expression, 8 μl of PCR-product and 1 μl CutSmart buffer were treated with 1 μl NlaIII (NEB, R0125S) for one hour at 37 °C. Mutant mRNA yielded fragments of 79 bp and 46 bp, whereas the WT fragment was

(17) JMJD1C forms condensates to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cellsProtein & CellMay 28, 2025Qian Chen, Saisai Wang, Juqing Zhang, Min Xie, Bin Lu, Jie He, Zhuoran Zhen, Jing Li, Jiajun Zhu, Rong Li, et al.precipitation. Dissolved DNA was digested by adding 1× CutSmart buffer, 0.2 mg/mL BSA and 40 Units NalIII (NEB, R0125L) for an overnight 37°C incubation. After this, DNA was ligated by adding 6,700 Units of T4 DNA ligase

(18) CTCF depletion decouples enhancer-mediated gene activation from chromatin hub formationNature Structural & Molecular BiologyMay 13, 2025Magdalena A. Karpinska, Yi Zhu, Zahra Fakhraei Ghazvini, Shyam Ramasamy, Mariano Barbieri, T. B. Ngoc Cao, Natalie Varahram, Abrar Aljahani, Michael Lidschreiber, Argyris Papantonis, et al.into three digest reactions of 5 million cells and digested with the NlaIII restriction enzyme (NEB, R0125L). After subsequent proximity ligation and DNA extraction, the three reactions were combined and sheared

(19) Single cell multiomics reveals drivers of metabolic dysfunction-associated steatohepatitismedRxivMay 11, 2025Weston Elison, Lei Chang, Yang Xie, Charlene Miciano, Qian Yang, Hannah Mummey, Ryan Lancione, Sierra Corban, Sadatsugu Sakane, Jacinta Lucero, et al.sheared using three restriction enzymes: DpnII (NEB, R0543L), MboI (NEB, R0147M), and NlaIII (NEB, R0125L), at 37 °C for 90 minutes on a ThermoMixer at 550 r.p.m. The three enzymes were then deactivated at

(20) An oncohistone-driven H3.3K27M/CREB5/ID1 axis maintains the stemness and malignancy of diffuse intrinsic pontine gliomaNat CommunApril 17, 2025Wei Zhou, Cheng Xu, Shuangrui Yang, Haocheng Li, Changcun Pan, Zhuang Jiang, Luyang Xie, Xiaohan Li, Huimin Qiao, Da Mi, et al.Tris-HCl (pH 7.5), 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA and Protease Inhibitor Cocktail. NlaIII (cat. #R0125, NEB) was used as primary restriction enzyme, DpnII (cat. #R0543) was used as secondary restriction

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Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007

NlaIII

NlaIII from New England Biolabs

Sequence

New England Biolabs

Citations (132)

Reviews

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Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis

NlaIII from New England Biolabs

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