Description
DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964. Performance and Quality Testing: Performance tested in nick translation reaction. Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer. Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 80 g/ml template primer, 32 M dTTP, 69 nM [3H]dTTP, and enzyme in 100 l for 30 min. at 37°C