NheI-HF from New England Biolabs

Product Specs
ItemNheI-HF
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR3131S
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Specifications/Features

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Already included in Biocompare under the itemid-946376

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Development of bicistronic plasmids and fusion proteins for clinical translation of tumor immune reprogrammingMolecular Therapy AdvancesJune 11, 2026Joanna Yang, Sabrina S. Chen, Ethan Idnani, Sydney R. Shannon, Kathryn Luly, Charina S. Fabilane, D. Scott Wilson, Jamie B. Spangler, Stephany Y. Tzeng, Jordan J. Greenstandard cloning methods. Restriction enzymes NheI (New England Biolabs [NE Biolabs]; NEB, catalog no. R3131S) and SalI (NEB, catalog no. R0138S) were used to remove the inserts from Clonal Genes (Twist Biosciences)

(2) A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networksJournal of Biological ChemistryApril 25, 2026Alicia Llorente, Gurpreet K. Arora, Ryan M. Loughran, Rabi Murad, Svetlana Maurya, Sophia Crabtree, Benji Portillo, Pratima Chapagain, Hundaol Huluka, Scott Eron, et al.(Addgene plasmid #91980, https://www.addgene.org/91980/) using NheI and EcoRI restriction enzymes (R3131S and R3101S, New England Biolabs), Phusion Hot Start II DNA Polymerase (F549S, Thermo Fisher Scientific)

(3) Using fibre photometry with a fluorescence resonance energy transfer-based biosensor to test efficacy of calpain inhibitors in vivoBrain CommunicationsApril 24, 2026Katherine J Robinson, Anita J Turner, Holly I Ahel, Andrea Kuriakose, Anastasiya Potapenko, Maxinne Watchon, Stuart K Plenderleith, Nicholas A Everett, Simon McMullan, Angela S Lairdcalpain sensor plasmid was excised using BsrGI (New England Biolabs #R3575S), Nhel (New England Biolabs #R3131S) restriction enzymes and rCutSmartTM Buffer (New England Biolabs #B6004S) and subcloned into an AAV

(4) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanellilab JF650-Chloroalkane (CA),Halo Tag,Lavis lab Enzymes,Enzymes,Enzymes BamHI-HF,New England Biolabs (NEB),R3131S BpiI,Thermo Fisher,FD1014 EcoRI-HF,NEB,R3101S HindIII-HF,NEB,R3104S Nhe-HF,NEB,R3131S NotI,NEB,R3189S Pfu

(5) Ligase-dependent and independent functions of the C-terminus of Mms21 contribute to optimal growth and genome stability in Saccharomyces cerevisiaeMol Biol CellApril 8, 2026Cheung Li, Anny Vo, Nkechinye Baadi, Yee Mon Thuintegrate the gene at the endogenous locus, pRS405 with MMS21 or mms21 was linearized by NdeI-HF (NEB R3131S) and transformed into BY4741 strains. The integration was verified by using these primers: Mms21_50up

(6) Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networksCell ReportsMarch 24, 2026Lin Lin, Naoto Kubota, Yi-Li Lam, Michelle Mingxue Song, Min Zhang, Sika Zhenga Double UP plasmid with standard 2-step PCR cloning techniques using the NheI (New England Biolabs R3131S) and MfeI (New England Biolabs R3589S) cut sites. Primers used for the first round of PCR were 5′- GATGACGATAAGGCGGAGAGCTGGCTG-3′

(7) Visualizing suborganellar lipid distribution using correlative light and electron microscopyNature Cell BiologyMarch 20, 2026H. Mathilda Lennartz, Suman Khan, Weihua Leng, Kristin Böhlig, Gunar Fabig, Yannick Kieswald, Falk Elsner, Nadav Scher, Michaela Wilsch-Bräuninger, Ori Avinoam, et al.AscI. The NES-PKI was inserted in the N-terminal of the two PABDs using NheI (New England Biolabs, R3131S) and NotI, mimicking the biosensor design from ref. 60. Transfection and live-cell imaging For transfection

(8) Control of Inflammatory Response by Tissue MicroenvironmentbioRxivMarch 18, 2026Zhongyang Wu, Scott D. Pope, Nasiha S. Ahmed, Diana L. Leung, Stephanie Hajjar, Qiuyu Yue, Diya M. Anand, Elizabeth B. Kopp, Daniel Okin, Weiyi Ma, et al.Mountant,Invitrogen,Cat# P36984 PacI,New England Biolabs,Cat# R0547S NheI-HF,New England Biolabs,Cat# R3131S MluI-HF,New England Biolabs,Cat# R3198S BamHI-HF,New England Biolabs,Cat# R3136L Propidium iodide,Sigma Aldrich

(9) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.DNA,LUMICKS,Cat# 00027 Nb.BbvCI,New England Biolabs,Cat# R0631S NheI-HF,New England Biolabs,Cat# R3131S BsrGI-HF,New England Biolabs,Cat# R3575S BsiWI-HF,New England Biolabs,Cat# R3553S BsaI-HF®v2,New England Biolabs

(10) Negative feedback regulation of STING signaling by TAX1BP1-directed GolgiphagyNature CommunicationsFebruary 11, 2026Sujit Suklabaidya, Suchitra Mohanty, Irene E. Reider, Jesse White, Dominic Colter, Sarah M. McCormick, Noula Shembade, Young Bong Choi, Christopher C. Norbury, Edward W. Harhajmanipulation pUltra-Hot-TAX1BP1 was generated by subcloning TAX1BP1 PCR product digested with Nhe1-HF (New England Biolabs; R3131S) and BamH1-HF (New England Biolabs; R3136S) into the pUltra-Hot vector. Silent mutations were introduced

(11) Regulatory grammar in human promoters uncovered by MPRA-based deep learningNatureFebruary 4, 2026Lucía Barbadilla-Martínez, Noud Klaassen, Vinícius H. Franceschini-Santos, Jérémie Breda, Hatice Yücel, Miguel Hernández-Quiles, Tijs van Lieshout, Carlos G. Urzua Traslaviña, Minh Chau Luong Boi, Maryam Akbarzadeh, et al.(Lucigen, ER81050), subsequently bead-purified and digested with EcoRI-HF (NEB, R3101S) and NheI-HF (NEB, R3131S) for 1 h at 37 °C. Fragments were ligated in NheI-HF/EcoRI-HF double-digested vector (an adaptation

(12) Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and influences the formation of reovirus factoriesJournal of VirologyDecember 23, 2025Raquel Tenorio, Gwen M. Taylor, Ana Cayuela, C. O. S. Sorzano, Isabel Fernández de Castro, Sara Y. Fernández-Sánchez, Alexa N. Roth, Xayathed Somoulay, Gavin S. Treadaway, Terence S. Dermody, et al.(5′-TGCTTACTCGAGTCAGTCATCTCCACCACACAGG-3′) to engineer untagged ANXA2 plasmids. PCR fragments were cloned into pcDNA3.1 using NheI (NEB, R3131S) and XhoI (NEB, R0146S). ANXA2 KO cells were either mock-transfected or transfected with engineered

(13) Synthesis of Bispecific Conjugates by ADP‐Ribosyl CyclasesAdvanced ScienceDecember 21, 2025Sunny H. Kim, Arshad J. Ansari, Lei Zhang, Guoyun Kao, Thuc Oanh Hoang, Zeyu Zhang, Srinivasarao Singireddi, Benjamin B. Katz, Yong Zhang(2060‐05) and goat anti‐human kappa‐FITC (2060‐02) were purchased from Southern Biotech, AL. NheI (R3131S) and EcoRI (R3101S) restriction enzymes and T4 DNA ligase (M0202S) were purchased from New England Biolabs,

(14) Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycleMolecular CellDecember 18, 2025Aviezer Silverman, Raneem Nashef, Reut Wasserman, Tamar Noy, Susan Born, Tianyou Yao, Yuncong Geng, Hila Rotbard, Adi Levkowitz, Yotam Kaufman, et al.,Thermo Fisher,Cat#AM9516 Mph1103I,Scientific Thermo Fisher,Cat#ER0731 NheI,Scientific New England Biolabs,Cat#R3131S HindIII,New England Biolabs,Cat#3104S RiboRuler Low Range RNA ladder,Thermo Fisher,Cat#SM1831 Low Range ssRNA ladder

(15) Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signalingDevelopmental BiologyDecember 1, 2025Agnese Kocere, Elena Chiavacci, Charlotte Soneson, Seth T. Jacobson, Emma N. Harrison, Kevin Manuel Méndez-Acevedo, Jacalyn S. MacGowan, Harrison H. Wells, Max S. Hiltabidle, Azhwar Raghunath, et al.5′-TTGCTAGCTCAGCGACGTCTCCGGTCT-3′ containing a NheI site (underlined). The PCR product was cloned with BamHI (R0136S, NEB), NheI (R3131S, NEB) restriction digest into Tol2kit vector 396 (pCS2FA:Tol2) (Kwan et al., 2007) cut with BamHI and

(16) Comprehensive analysis of yeast +1 ribosomal frameshifting unveils a novel stimulator supporting two distinct frameshifting mechanismsNucleic Acids ResearchNovember 28, 2025Darren A Fenton, Maria Bożko, Michał I Świrski, Gary Loughran, Martina M Yordanova, Joanna Kufel, John F Atkins, Pavel V Baranovframeshifting heptamers, annealed oligos were ligated into the NdeI- (NEB, #R0111S) and NheI- (NEB, #R3131S) digested vector with T4 DNA ligase (NEB, #M0202S) and incubated overnight at room temperature in a

(17) Strategies to Screen and Evaluate Brain Targeting Antibodies Using an iPSC-Derived Blood–Brain Barrier ModelAntibodies (Basel)November 26, 2025Eun Seo Choi, Sophia Sahota, Emily Burnham, Yunfeng Ding, Eric V. Shustacloned into the place of WT 46.1 insert in the pIRES-46.1-rabbitFc plasmid [24] using the NheI (NEB R3131S) and AgeI (NEB R3552S) restriction enzymes. For the histidine point mutants, gene blocks for each variant

(18) The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damageThe EMBO JournalNovember 20, 2025Alexandra Boegli, Elliott M Bernard, Louise Lacante, Gaël Majeux, Ella Hartenian, Vanessa Mack, Petr Brozthe CMV promoter was replaced by an EF1a promoter using SnaBI-NheI (New England Biolabs #R0130S and #R3131S) restriction digest and InFusion cloning, as well as the Puromycin resistance cassette was replaced

(19) OSA Initiates Histone Lactylation That Drives PDE4B / FUS / AGT Axis to Pulmonary HypertensionCell ProliferationNovember 17, 2025Li Yang, Qing Ni, Yan He, Shijie Liu, Lulu Gan, Anni Dai, Yang Hu, Qian Liu, Xueling Yang, Jiqian Li, et al.Vector Construction The parental plasmid pGFP‐C1‐FUS (Addgene) was digested with NheI‐HF (20 U/μg, NEB, R3131S) and SacI (20 U/μg, NEB, R0156S) at 37°C for 3 h. The **RFP cDNA** (Clontech, #632504) was ligated into

(20) Functional analysis of TWIST1 domains regulating smooth muscle cell phenotypeFrontiers in Cardiovascular MedicineOctober 31, 2025Danielle C. M. Dy, Thiel Lehman, Adrian Othon, Mitesh Rathod, William J. Polacheck, Robert WirkaNEB HiFi DNA Assembly protocol (E2621l; New England Biolabs). Fragments were inserted into a NheI-HF (R3131S, NEB) digested pLJM1-EGFP expression vector (19319, Addgene) modified with a T2A linker between the

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4/23/2018Diagnostic Restriction Mapping Of The Plasmid Construct With NheI-HF Triveni MahendrappaI have designed and constructed a donor plasmid of size approximately 19.4 Kb for cloning purposes. This plasmid prepped from E. coli DH5 alpha cells were subjected to NheI-HF restriction digestion to... Read Review

NheI-HF

NheI-HF from New England Biolabs

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New England Biolabs

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NheI-HF from New England Biolabs

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