Neural Tissue Dissociation Kit (P) from Miltenyi Biotec

(1) BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic periodProtein & cellJanuary 3, 2024Li Z, Liu G, Yang L, Sun M, Zhang Z, Xu Z, Gao Y, Jiang X, Su Z, Li X, et al.dissociated into a single-cell suspension using a Papain Cell Dissociation Kit (Miltenyi Biotec, catalog no. 130-092-628) according to the manufacturer’s instructions. CellTrace Yellow labeled single-cells were sorted using

(2) Peripherally derived myeloid cells induce disease-dependent phenotypic changes in microgliaFront Cell NeurosciDecember 14, 2023Estrid Thougaard, Brianna Carney, Agnieszka Wlodarczyk, Roberta Brambilla, Kate Lykke Lambertsendissociated into a single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, #130-092-628) followed by myelin removal with debris removal solution (Miltenyi Biotec, #130-109-398). Microglia

(3) The effects of chronic high-dose morphine on microgliosis and the microglial transcriptome in rat spinal cordMolecular PainDecember 1, 2023Fredrik H G AhlstrÃ M, Hanna Viisanen, Leena Karhinen, Kert Mã Tlik, Kim J Blomqvist, Tuomas O Lilius, Yulia A Sidorova, Vinko Palada, Pekka V Rauhala, Eija A KalsoThermoFisher, United States). The tissue was dissociated using the Neural Tissue Dissociation Kit (P) (#130-092-628, Miltenyi Biotec, Germany) and gentleMACS™ Dissociator (Miltenyi Biotec, Germany), according to the

(4) Autophagy drives the conversion of developmental neural stem cells to the adult quiescent stateNature CommunicationsNovember 24, 2023Isabel Calatayud-Baselga, Lucía Casares-Crespo, Carmina Franch-Ibáñez, José Guijarro-Nuez, Pascual Sanz, Helena Miraday 3 or day 21 C57BL/6JRccHsd mice were dissociated using Neural Tissue Dissociation Kit (Miltenyi, 130-092-628) with gentle MACS Octo Dissociator (Miltenyi, 130-095-937). GLAST-positive cells were isolated by MACS.

(5) Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammationFrontiers in ImmunologyNovember 22, 2023Wouter Claeys, Daan Verhaege, Griet Van Imschoot, Elien Van Wonterghem, Lore Van Acker, Laura Amelinck, Federico F. De Ponti, Charlotte Scott, Anja Geerts, Christophe Van Steenkiste, et al.dissociated into single cell suspensions using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130‐092‐628) as described previously (41). In short, cells were enzymatically dissociated using activated enzyme

(6) Germinal matrix hemorrhage induces immune responses, brain injury, and motor impairment in neonatal ratsJournal of Cerebral Blood Flow & MetabolismNovember 1, 2023Xiaoli Zhang, Jing Yuan, Shan Zhang, Wendong Li, Yiran Xu, Hongwei Li, Lingling Zhang, Xi Chen, Wenjun Ding, Jinjin Zhu, et al.injured brains were quickly removed and cut into pieces and the Neural Tissue Dissociation Kit (Cat#130092628, Miltenyi Biotec) was used to acquire a single cell suspension using a MACS Octo Dissociator with heaters.

(7) Microglial-Targeted nSMase2 Inhibitor Fails to Reduce Tau Propagation in PS19 MicePharmaceuticsSeptember 21, 2023Huang M, Tallon C, Zhu X, Huizar KDJ, Picciolini S, Thomas AG, Tenora L, Liyanage W, Rodà F, Gualerzi A, et al.target engagement via the microglial isolation beads method. A neural tissue dissociation kit (P) (#130-092-628, Miltenyi Biotec, Gaithersburg, MD, USA) was used, following the manufacturer’s instructions. Briefly,

(8) PKC modulator bryostatin-1 therapeutically targets CNS innate immunity to attenuate neuroinflammation and promote remyelinationbioRxivSeptember 3, 2023Payam Gharibani, Efrat Abramson, Shruthi Shanmukha, Matthew D. Smith, Wesley H. Godfrey, Judy J. Lee, Jingwen Hu, Maryna Baydyuk, Marie-France Dorion, Xiaojing Deng, et al.was then resuspended in Enzyme P using Neural Tissue Dissociation Kit P (Miltenyi Biotec, catalog no. 130–092-628) and was spun at 37°C for 15 min, followed by Enzyme A addition. The mixture was triturated 15–20 times

(9) Inferring and perturbing cell fate regulomes in human brain organoidsNatureSeptember 1, 2023Fleck JS, Jansen SMJ, Wollny D, Zenk F, Seimiya M, Jain A, Okamoto R, Santel M, He Z, Camp JG, et al.suspensions were acquired by dissociation of the organoids with a papain-based dissociation (Miltenyi Biotec, 130-092-628). Prewarmed papain solution (2 ml) was added to the organoids and incubated for 15 min at 37 °C. Enzyme

(10) sox1a:eGFP transgenic line and single-cell transcriptomics reveal the origin of zebrafish intraspinal serotonergic neuronsiScienceAugust 18, 2023Fushun Chen, Melina Köhler, Gokhan Cucun, Masanari Takamiya, Caghan Kizil, Mehmet Ilyas Cosacak, Sepand Rastegarassays,Critical commercial assays,Critical commercial assays ,, Neural Tissue Dissociation Kit (P),Miltenyi,Cat#130-092-628 10x Chromium Kit,10x Genomics,Cat#1000092 HCR RNA-FISH,"Molecular Instruments, Inc.",https://www.molecularinstruments.com/ NGS

(11) A sex-specific thermogenic neurocircuit induced by predator smell recruiting cholecystokinin neurons in the dorsomedial hypothalamusNat CommunAugust 15, 2023Predrag Jovanovic, Allan-Hermann Pool, Nancy Morones, Yidan Wang, Edward Novinbakht, Nareg Keshishian, Kaitlyn Jang, Yuki Oka, Celine E. Rieraand cell dissociation was performed using Neural Tissue Dissociation kit (P) (Miltenyi Biotec, Cat#130-092-628) following manufacturer protocol with the use of gentleMACS Octo Dissociator (Miltenyi Biotec, Cat#130-095-937).

(12) DOT1L activity affects neural stem cell division mode and reduces differentiation and ASNS expressionEMBO reportsAugust 3, 2023Appiah B, Fullio CL, Ossola C, Bertani I, Restelli E, Cheffer A, Polenghi M, Haffner C, Garcia-Miralles M, Zeis P, et al.assays,Commercial assays,Commercial assays Neural Tissue Dissociation Kit (P),Miltenyi Biotec,Cat# 130‐092‐628 Click‐it EdU Detection Kit,Molecular Probes,Cat# C10340 Deposited data,Deposited data,Deposited data Single‐cell

(13) cGAS-STING drives ageing-related inflammation and neurodegenerationNatureAugust 2, 2023Muhammet F. Gulen, Natasha Samson, Alexander Keller, Marius Schwabenland, Chong Liu, Selene Glück, Vivek V. Thacker, Lucie Favre, Bastien Mangeat, Lona J. Kroese, et al.The brain pieces were further dissociated using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-092-628) according to the manufacturer’s instructions. Dissociated cells were filtered through a 100 µm cell

(14) An independent regulator of global release pathways in astrocytes generates a subtype of extracellular vesicles required for postsynaptic functionScience advancesJune 23, 2023Levy-Myers R, Daudelin D, Na CH, Sockanathan S.WT mice were dissected and pooled. The Neural Tissue Dissociation Kit with Papain (Miltenyi Biotec, 130-092-628) was used to generate single-cell suspensions following the manufacturer’s protocol for manual dissociation.

(15) Deficiency in the omega-3 lysolipid transporter Mfsd2a leads to aberrant oligodendrocyte lineage development and hypomyelinationJournal of Clinical InvestigationJune 15, 2023Vetrivel Sengottuvel, Monalisa Hota, Jeongah Oh, Dwight L. Galam, Bernice H. Wong, Markus R. Wenk, Sujoy Ghosh, Federico Torta, David L. Silverexcised, and dissociation was performed with a Papain Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) following the manufacturer’s instructions. A single-cell suspension was then FACS sorted for GFP+ cells

(16) Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in MiceBiologyJune 12, 2023Lund MC, Ellman DG, Nielsen PV, Raffaele S, Fumagalli M, Guzman R, Degn M, Brambilla R, Meyer M, Clausen BH, et al.conditions. Cortices were processed using Neural Tissue Dissociation Kit (P) (Miltenyi Biotech, cat. no. 130-092-628, Lund, Sweden) according to the manufacturer’s protocol. Cell suspensions were filtered through 70 μm

(17) Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent mannersThe Journal of biological chemistryJune 1, 2023Xu Y, Kusuyama J, Osana S, Matsuhashi S, Li L, Takada H, Inada H, Nagatomi R.brain tissues were dissociated into single cells by using the MACS Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec), gentleMACS C tubes (130-093-237, Miltenyi Biotec), and the gentleMACS Octo Dissociator with Heaters

(18) Multimodal chromatin profiling using nanobody-based single-cell CUT&TagNature BiotechnologyJune 1, 2023Marek Bartosovic, Gonçalo Castelo-Brancodissociated into a single-cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturer’s protocol. For mice older than P7, myelin was removed using debris removal

(19) The transcriptional co-activator Yap1 promotes adult hippocampal neural stem cell activationThe EMBO journalJune 1, 2023Fan W, Jurado-Arjona J, Alanis-Lobato G, Péron S, Berger C, Andrade-Navarro MA, Falk S, Berninger B.enzymatically dissociated using the Neural Tissue Dissociation Kit (Miltenyi Biotec, Germany, cat# 130‐092‐628) according to the manufacturer's instructions. For the fluorescence‐activated cell sorting (FACS), the

(20) Protocol to establish a genetically engineered mouse model of IDH1-mutant astrocytomaSTAR ProtocolsJune 1, 2023Diana D. Shi, Joyce H. Lee, Adam C. Wang, Januka Khanal, Wenhua Gao, William G. Kaelin, Samuel K. McBrayerBiosystems,Cat# 39477001 Slide wwarmer,Thermo Fisher,Cat# 12-594 Neural Tissue Dissociation Kit,Miltenyi,Cat# 130-092-628 Bruker BioSpec 7T/30 cm USR horizontal bore Superconducting Magnet System,Bruker,https://www.bruker.com/en/products-and-solutions/preclinical-imaging/mri/biospec/biospec-70-30-and-94-30.html

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(1)

3/6/2018Neural Tissue Dissociation Kit Alex RayWe use this kit to dissociate mouse brain tissue to isolate microglia for in vitro experiments. The kit provides an excellent way to dissociate brain tissue to isolate cells of interest.
(1)

7/25/2017Kit For Neuronal Tissue Processing Chris C Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. One of the mouse lines we generated expresses human cell receptors which allows the cells to ... Read Review
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6/30/2016Best Kit for Embryonic and Postnatal Brain Tissue Yi PangThis kit works very well for embryonic and postnatal rat brain tissue ( day 1 to day 7 rat tested). Compared to traditional trypsin-based method, this kit yields more total cells as well as viable ... Read Review
(1)

1/16/2015Quick and Easy Neural Tissue Dissociation Kit Kristi AndersonI really enjoy using this kit to isolate neurons from mouse brain. The entire protocol is takes less than a couple hours and the instructions are easy to follow.

Neural Tissue Dissociation Kit (P) (for 50 preparations)

Neural Tissue Dissociation Kit (P) from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Neural Tissue Dissociation Kit (P) from Miltenyi Biotec

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