NdeI from New England Biolabs

Product Specs
ItemNdeI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0111L
Quantity20000 units
ApplicationsDNA Manipulation
Concentration20 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

CA/TATG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.,ThermoFisher Scientific,Cat#FD0324 Eco32I,ThermoFisher Scientific,Cat#FD0304 KpnI,New England Biolabs,Cat#R3142L NdeI,New England Biolabs,Cat#R0111L BamHI,New England Biolabs,Cat#R0136L NcoI,New England Biolabs,Cat#R0193L FastAP,ThermoFisher Scientific,Cat#EF0651 "RNase Inhibitor, Murine

(2) Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assaySTAR ProtocJuly 15, 2025Thomas M. Groseclose, Zoe K. Taylor, Lexy A. Lujan, Taraka Dale, Hau B. NguyenHPLC,Supelco,Cat#49685-100ML BamHI Enzyme,New England Biolabs,Cat# R0136L NdeI Enzyme,New England Biolabs,Cat# R0111L DNaseI Amp grade,Invitrogen,Cat#18068-015 DeepVent exo(−) polymerase + ThermoPol buffer,New England Biolabs

(3) Structural and virological identification of neutralizing antibody footprint provides insights into therapeutic antibody design against SARS-CoV-2 variantsCommun BiolMarch 22, 2025Yuki Anraku, Shunsuke Kita, Taishi Onodera, Akihiko Sato, Takashi Tadokoro, Shiori Ito, Yu Adachi, Ryutaro Kotaki, Tateki Suzuki, Jiei Sasaki, et al.KOD-Plus DNA polymerase (TOYOBO, KOD-201). The fragments were digested with NdeI (New England Biolabs, R0111L) and HindIII (New England Biolabs, R0104L), ligated into pET22b. The expression plasmid of the scFv

(4) Protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid systemSTAR ProtocolsMarch 1, 2025Yixuan Hu, Yutao Chen, Yu Zhang, Zhiquan Liu, Jianzhen Liligase,Takara,2011A LR Clonase II Plus,Invitrogen,12538120 XhoI,NEB,R0146V SmaI,NEB,R0141V NdeI,NEB,R0111L BseRI,NEB,R0581L NotI,NEB,R0189L Ampicillin (Amp),BioFroxx,1146GR001 Kanamycin (Kan),Solarbio,K8020 Chloramphenicol

(5) Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processingThe EMBO JournalFebruary 24, 2025Geng-Jen Jang, Alex L Payne-Dwyer, Robert Maple, Zhe Wu, Fuquan Liu, Sergio G Lopez, Yanning Wang, Xiaofeng Fang, Mark C Leake, Caroline Deanmicrospheres,Thermo Fisher Scientific,T7280 proteinase K,Thermo Fisher Scientific,AM2546 DdeI,New England Biolabs,R0111L NdeI,New England Biolabs,R0175 XbaI,New England Biolabs,R0145L RNAse III,Thermo Fisher Scientific,AM2290 Dynabeads Protein G for Immunoprecipitation

(6) Paradoxical Activation of Oncogenic Signaling as a Cancer Treatment StrategyCancer DiscovMarch 26, 2024Matheus Henrique Dias, Anoek Friskes, Siying Wang, Joao M. Fernandes Neto, Frank van Gemert, Soufiane Mourragui, Chrysa Papagianni, Hendrik J. Kuiken, Sara Mainardi, Daniel Alvarez-Villanueva, et al.sample (50 μg each, using all material) and fragmented at 37°C overnight, using 100 U Ndel enzyme (R0111L) and 50 μL 10X cutSmart buffer (B7204S) from New England Biolabs, supplemented to 500 μL with nuclease-free

(7) Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphaeCurrent BiologyJuly 10, 2023Ingrid Richter, Philipp Wein, Zerrin Uzum, Claire E. Stanley, Jana Krabbe, Evelyn M. Molloy, Nadine Moebius, Iuliia Ferling, Falk Hillmann, Christian Hertweck,New England Biolabs,CAT#M0486L Ethidium bromide,New England Biolabs,CAT#E1510 NdeI,New England Biolabs,CAT#R0111L AflII,New England Biolabs,CAT#R0520L BstBI,New England Biolabs,CAT#R0519L Chloramphenicol,Roth,CAT#3886.1 Potato

(8) easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from ProteobacteriaBio ProtocJuly 5, 2023Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bode/PstI HF (New England Biolabs, catalog numbers: R0140L/R3140L)NdeI (New England Biolabs, catalog number: R0111L)Monarch Genomic DNA Purification kit (New England Biolabs, catalog number: T3010L)100 bp DNA ladder

(9) easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from ProteobacteriaBIO-PROTOCOLJuly 5, 2023Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bode/PstI HF (New England Biolabs, catalog numbers: R0140L/R3140L)NdeI (New England Biolabs, catalog number: R0111L)Monarch Genomic DNA Purification kit (New England Biolabs, catalog number: T3010L)100 bp DNA ladder

(10) Systematic screening identifies ABCG2 as critical factor underlying synergy of kinase inhibitors with transcriptional CDK inhibitorsBreast Cancer ResearchMay 5, 2023Vera E. van der Noord, Wanda van der Stel, Gijs Louwerens, Danielle Verhoeven, Hendrik J. Kuiken, Cor Lieftink, Melanie Grandits, Gerhard F. Ecker, Roderick L. Beijersbergen, Peter Bouwman, et al.sample (50 µg each, using all material) and fragmented at 37 °C overnight, using 50 U Ndel enzyme (R0111L), 50U Pstl-HF enzyme (R3140L), and 50 µL 10X cutSmart® buffer (B7204S) from New England Biolabs, supplemented

(11) Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approachSTAR ProtocolsSeptember 16, 2022Tomas Pachano, Alvaro Rada-Iglesias,New England Biolabs,Cat: R0133L MluI-HF,New England Biolabs,Cat: R3198L NdeI,New England Biolabs,Cat: R0111L NotI-HF,New England Biolabs,Cat: R0189L BbsI,New England Biolabs,Cat: R0539L T4 DNA Ligase,New England Biolabs

(12) Purification and cryo-EM structure determination of VCP/p97 dodecamers from mammalian and bacterial cellsSTAR ProtocolsJune 17, 2022Guimei Yu, Yunpeng Bai, Zhong-Yin Zhang,New England Biolabs,Cat#M0398S HindIII,New England Biolabs,Cat#R0104L BamHI,New England Biolabs,Cat#R0136L NdeI,New England Biolabs,Cat#R0111L T4 DNA ligase,New England Biolabs,Cat#M0202L Fast DNA Ladder,New England Biolabs,Cat#N3238S DMEM

(13) GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cellseLifeMay 13, 2022Fernandez RJ, Gardner ZJG, Slovik KJ, Liberti DC, Estep KN, Yang W, Chen Q, Santini GT, Perez JV, Root S, et al.R0568L, "Peptide, recombinant protein",MseI,NEB,CAT# R0525L, "Peptide, recombinant protein",NdeI,NEB,CAT# R0111L, "Peptide, recombinant protein",rSAP,NEB,CAT# M0371L, "Chemical compound, drug",CDP-Star,Roche,CAT#

(14) "MYC assembles and stimulates topoisomerases 1 and 2 in a ""topoisome"""Molecular cellJanuary 6, 2022Das SK, Kuzin V, Cameron DP, Sanford S, Jha RK, Nie Z, Rosello MT, Holewinski R, Andresson T, Wisniewski J, et al.,New England Biolabs Inc.,Cat# M0379S XhoI,New England Biolabs Inc.,Cat# R0146L NdeI,New England Biolabs Inc.,Cat# R0111L EcoRI,New England Biolabs Inc.,Cat# R0101L BamHI,New England Biolabs Inc.,Cat# R0136L "IPTG ≥ 99%

(15) A common binding motif in the ET domain of BRD3 forms polymorphic structural interfaces with host and viral proteinsStructureAugust 1, 2021Sriram Aiyer, G.V.T. Swapna, Li-Chung Ma, Gaohua Liu, Jingzhou Hao, Gordon Chalmers, Brian C. Jacobs, Gaetano T. Montelione, Monica J. Rothchloride,Cambridge Isotope,Cat # NLM-713-PK 13C-glucose,Cambridge Isotope,Cat # CLM-1396-PK NdeI,NEB,Cat # R0111L SpeI,NEB,Cat # R0133S EcoRI HF,NEB,Cat # R3101T HindIII HF,NEB,Cat # R3104T EDTA,JT Baker,Cat #

(16) Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2STAR ProtocolsDecember 30, 2020Jiuyang Liu, Zhaoyu Xue, Kendra R. Vann, Xiaobing Shi, Tatiana G. KutateladzeMiller",Thermo Fisher Scientific,Cat. # BP9724-500 NdeI restriction endonucleases,New England Biolabs,Cat. # R0111L EcoRI restriction endonucleases,New England Biolabs,Cat. # R0101L Dpn I Enzyme,Agilent Technologies,Cat.

(17) Methanol metabolism in the acetogenic bacteriumAcetobacterium woodiiEnvironmental MicrobiologyDecember 1, 2018Florian Kremp, Anja Poehlein, Rolf Daniel, Volker MÃ¼ller

(18) Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosisCell reportsOctober 2, 2018Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P, et al.Platinum Taq DNA polymerase,ThermoFisher Scientific,Cat# 10966034 Restriction enzymes,New England Biolabs(UK) Ltd- /,Cat #R3193S; R3182L; R0111L; R3136L; R3142L LigaFast Rapid DNA Ligation System,Promega,Cat# M8221 SignalFire Elite ECL Reagent,Cell Signaling Technology

(19) Double mimicry evades tRNA synthetase editing by toxic vegetable-sourced non-proteinogenic amino acidNature CommunicationsDecember 1, 2017Youngzee Song et al.

(20) Primer Extension Reactions for the PCR- based a- complementation AssayBIO-PROTOCOLJune 20, 2015Vasudevan Achuthan, Jeffrey J DeStefanoloading dye (New England Biolabs, catalog number: R3101L)NdeI (New England Biolabs, catalog number: R0111L)T4 polynucleotide kinase (PNK) (New England Biolabs, catalog number: M0201L)10x T4 polynucleotide kinase

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NdeI

NdeI from New England Biolabs

Sequence

New England Biolabs

Citations (23)

Reviews

NdeI from New England Biolabs

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