NdeI from New England Biolabs

Product Specs
ItemNdeI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0111S
Concentration20 u/µL
Quantity4000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CA/TATG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Decoding strain level interactions between Vibrio cholerae and gut microbiotaVirulenceJuly 30, 2026Guozhong Chen, Shucheng Li, Qinghua Zhao, Yanfei Han, Zhiyuan Zhang, Zhi Liu(Addgene, Cat#75286) was linearized using restriction enzymes NdeI (New England Biolabs (Beijing) Ltd., Cat#R0111S) and BamHI (New England Biolabs (Beijing) Ltd., Cat# R3136S). The katE-like gene sequence was amplified

(2) A variant in human leucine-rich repeat and coiled-coil domain-containing 1 (LRRCC1) elevates meiotic aneuploidy in oocytesResearchSquareJuly 5, 2026Marlena Duke, Karen Schindlervariant plasmids were linearized with NdeI restriction digest (New England Biolabs, Ipswich, MA, USA, #R0111S). All linearized plasmids were purified (QIAquick PCR Purification Kit, Qiagen, Aarhus, Denmark, #28104).

(3) CAGE-TRX expands the scope of time-resolved crystallography through genetically encoded active-site photocagingbioRxivJune 29, 2026Clyde A. Smith, Ailiena O. Maggiolo, Chasity Jonosko, Maura E. Charette, Nilakshi Paul, Marta Toth, Guillermo Calero, Sierra M. Carr, Silvia Russi, Sergei B. Vakulenko, et al.generated via restriction enzyme digest of pBAD-sfGFP-pylT (2000 ng) using NcoI (NEB R013S) and NdeI (NEB R0111S) and subsequent gel purification. The expression constructs pBAD-GES5-K73TAG-pylT, pBAD-GES5-K234TAG-pylT,

(4) Insights into the Mechanism Underlying the Alkane Dehydrogenation Capability of Rhodococcus sp. KSM-B-3MMicroorganismsJune 2, 2026Nurit Edri, Keren Buhnik-Rosenblau, Anthony Cohen, Shlomit Hakim, Fabian Glaser, Guy Horev, Jeffrey Bruffaerts, Ilan Marek, Yechezkel KashiTable S1). The purified PCR product was ligated into the pDD120 backbone digested in its NdeI (Cat. No. R0111S, New England Biolabs, Ipswich, MA, USA) and ApaI (Cat. No. R0114S, New England Biolabs, Ipswich, MA,

(5) DNA Cut-Ligation Cyclization Surpasses Jacobson–Stockmayer J-Factor Expectations by over ThreefoldBiomoleculesMay 22, 2026Roman Teo Oliynyk, George M. Churchband in lane 3. In lane 5, we performed a single-cut digestion of the purified monomer using NdeI (NEB R0111S), which cuts approximately in the middle of the original dsDNA sequence (see 452bp-Circularized.gbk

(6) A tumor-intrinsic WNT-inhibitory NOTUM program drives immune resistance in microsatellite stable colorectal cancerCell Rep MedMay 19, 2026Julian Chua, Arshdeep Kaur, Fynnis Mitchell, Anneke Korver, Parnika Sakthivel, Elleine Allapitan, Mahdin Uddin, Areeba Maqsood, Naomi Chege, Aralia Leon-Coria, et al.,Takara Bio,R045B Puromycin,Thermofisher,AAJ67236XF AP20187,Sigma,SML2838-1 Tween 20,Sigma,P2287 Circular Coverslips,Thermofisher,174969 NdeI,NEB,R0111S SacI-HF,NEB,R3156S BsaBI,NEB,R0537S SphI,NEB,R0182S BsmBI-v2,NEB,R0739S ,, Critical commercial

(7) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapat#R3142S) and NotI (NEB, #R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S); Δ TATA promoter using SbfI-HF (NEB, #R3642S) and BglII. For insertion of

(8) SHP-1 agonist SC-43 limits methicillin-resistant Staphylococcus aureus infection through inhibition of heme biosynthesisEMBO Molecular MedicineApril 10, 2026Yini Huang, Yan Ye, Xinmei Zhu, Dengpan Liang, Ruiqin Cui, Xiaopeng Yuan, Xitao Li, Quanming Zou, Haibo Li, Wei Huangtag,Thermo Fisher,90064B Acetonitrile,Thermo Fisher,CAS 75-05-8 Formic acid,Pierce,28905 NdeI,NEB,R0111S XhoI,NEB,R0146S E. coli DH5α,Sangon Biotech,B528413 E. coli BL21,Sangon Biotech,B528419 Kanamycin,TargetMol,T64922 IPTG,BBI

(9) A Vertebrate Toxin-Antidote System That Sabotages Mouse EmbryogenesisbioRxivMarch 28, 2026Duilio M.Z.A. Silva, Morgan Skinner, Takaya Totsuka, Takashi AkeraN-terminal mCherry for the SP110 construct. Plasmid DNA linearized by NdeI (New England Biolabs, cat# R0111S) were used as a template to synthesize cRNAs with the T7 mMessage mMachine Kit (Ambion, cat# AM1340),

(10) Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessusAntimicrobial Agents and ChemotherapyMarch 2, 2026Tobias Funck, Kerry McGowen, Mark R. Sullivan, Samuel Zinga, Ian D. Wolf, Dennis Nurjadi, Claudia M. Denkinger, Eric J. RubinMAB_2736c (PCR amplified from ATC19977 gDNA) or mNeonGreen using standard restriction cloning with NdeI (R0111S, NEB) and PacI (R0527S, NEB). PURExpress reactions were set up following the manufacturer’s protocol

(11) Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell ReceptorsResearch SquareFebruary 18, 2026Funsho J. Ogunshola, Nishant K. Singh, Vincent Butty, Anurag R. Mishra, Zacharia Habte, Liza Vecchiarello, Ahmed Fahad, Kate B. Juergens, Sophia Cheever, Marius Allombert, et al.system and subsequently cloned into the pET22b(+) vector (Cat. 69744–3, Novagen) between the NdeI (Cat. R0111S, NEB) and Xhol (Cat. R0146S, NEB) restriction enzyme sites. The transformed E. coli were induced for

(12) Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell ReceptorsbioRxivFebruary 2, 2026Funsho J. Ogunshola, Nishant K. Singh, Vincent Butty, Anurag R. Mishra, Zacharia Habte, Liza Vecchiarello, Ahmed Fahad, Kate B. Juergens, Sophia Cheever, Marius Allombert, et al.system and subsequently cloned into the pET22b(+) vector (Cat. 69744–3, Novagen) between the NdeI (Cat. R0111S, NEB) and Xhol (Cat. R0146S, NEB) restriction enzyme sites. The transformed E. coli were induced for

(13) Solid-phase DNA-encoded library synthesis: a master builder’s instructionsNature ProtocolsFebruary 1, 2026Anjali Dixit, Brian M. Paegel(20,000 U/ml, New England Biolabs, Cat. no. R3193S) NdeI (20,000 U/ml, New England Biolabs, Cat. no. R0111S) Quality Control Acrylamide/Bis 19:1 (40% (w/v) solution, Bio-Rad, Cat. no. 1610144) Ammonium persulfate (APS

(14) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. Brucecamsap2a digoxigenin-labeled antisense riboprobe was made by digesting the plasmid with NdeI (NEB, R0111S) and transcribing with T7 RNA polymerase using the Dig RNA labeling kit. The probe was purified using

(15) Structural analyses and substrate profiling of PPEP-3 provide new insights into the molecular basis of Pro-Pro endopeptidase specificityiScienceJanuary 16, 2026Bart Claushuis, Fabian Wojtalla, Lisa Papenhagen, Robert A. Cordfunke, Arnoud H. de Ru, Hans C. van Leeuwen, Jeroen Corver, Paul J. Hensbergen, Ulrich Baumannpositions are varied),This paper,N/A Restriction endonuclease NdeI,New England Biolabs,Catalog No R0111S Restriction endonuclease XhoI,New England Biolabs,Catalog No R0146S Restriction endonuclease DpnI,New England Biolabs

(16) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. Cravattexpression were synthesized by IDT. The ORF of TRMT112 was cloned into a pACYCDuet-1 vector using NdeI (NEB, R0111S) and BglII (NEB, R0144S) restriction and Gibson assembly (NEB, E2621S). Mutagenesis was carried out

(17) Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and compositionNucleic Acids ResDecember 11, 2025Hui Zhang, Enes Ugur, Christian Hake, Hector Romero, Maria Arroyo, Marah Mahmoud, Frederik Lermyte, Heinrich Leonhardt, M Cristina Cardosopolymerase chain reaction (PCR) from peMBD2G (pc2399) using primer pairs containing NdeI restriction enzyme (NEB, R0111S) for the forward primer and EcoRI restriction enzyme (NEB, R0101S) for the reverse primer (Supplementary

(18) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce DemplerCutSmart buffer (NEB, B6004S) in a 25 μl volume. After a 30-min incubation at 37°C, 20 units of NdeI (NEB, R0111S) were added to the reaction, followed by an additional 1-h incubation at 37°C. A separate set of reactions

(19) Antisense oligonucleotide therapy mitigates autosomal dominant progressive hearing loss in a murine model of human DFNA2Molecular TherapyDecember 3, 2025Seung Hyun Jang, Jae Won Roh, Kyung Seok Oh, Sun Young Joo, Jung Ah Kim, Se Jin Kim, Jae Young Choi, Jinsei Jung, Yeonjoon Kim, Jinwoong Bok, et al.Kcnq4-genotype-rev: 5′-CATCAGGTTCTTGCGAACCT-3′). Then, the PCR products were digested with NdeI (NEB, catalog no. R0111S) for 1–2 h at 37°C and subjected to 1.5% agarose gel electrophoresis to determine genotype. Inner ear

(20) Comprehensive analysis of yeast +1 ribosomal frameshifting unveils a novel stimulator supporting two distinct frameshifting mechanismsNucleic Acids ResearchNovember 28, 2025Darren A Fenton, Maria Bożko, Michał I Świrski, Gary Loughran, Martina M Yordanova, Joanna Kufel, John F Atkins, Pavel V BaranovFor insertion of short frameshifting heptamers, annealed oligos were ligated into the NdeI- (NEB, #R0111S) and NheI- (NEB, #R3131S) digested vector with T4 DNA ligase (NEB, #M0202S) and incubated overnight

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4/20/2018Diagnostic Restriction Mapping Of The Plasmid Construct PTS-Del With NdeI Triveni MahendrappaI routinely construct series of several plasmids for cloning and expression purposes. In such cases, there is a necessity to check constructed plasmids quickly by diagnostic restriction mapping using ... Read Review

NdeI

NdeI from New England Biolabs

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New England Biolabs

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NdeI from New England Biolabs

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