NcoI from New England Biolabs

Product Specs
ItemNcoI
CompanyNew England Biolabs
Price $286.00 Inquire
Catalog NumberR0193L
Quantity5000 units
Concentration10-50 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CATGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Changes in EGFR activity following CRISPR/Cas9-editing of the EGF binding domainScientific ReportsJanuary 31, 2026Jelena Popović, Anna Hahut, Gabriel E. Torres, Andrew Vincent, Nashaly Soto-Echevarria, Brian Wray, Elizabeth T. Bartom, Tatjana Paunesku, Chelain R. Goodman, Gayle E. WoloschakPCR purification kit (K310001, Invitrogen, MA, United States) and RFLP analysis was done using NcoI (R0193S, New England Biolabs, MA, United States) and SpeI (R3133S, New England Biolabs, MA, United States) enzymes.

(2) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.,New England Biolabs,Cat#R3142L NdeI,New England Biolabs,Cat#R0111L BamHI,New England Biolabs,Cat#R0136L NcoI,New England Biolabs,Cat#R0193L FastAP,ThermoFisher Scientific,Cat#EF0651 "RNase Inhibitor, Murine",New England Biolabs,Cat#M0314L T4 RNA Ligase 1 (ssRNA Ligase

(3) Global Evaluation of Congenital Heart Disease-Associated Non-Coding VariantsResearch SquareJanuary 7, 2026Edwin G. Rodríguez-Martínez, Shreya Peña-Martínez, Joshua G. Medina-Feliciano, Joshua Medina-Feliciano, Lois G. Parks, Marissa Granitto, Diego A. Pomales-Matos, Jean L. Pomales-Matos, Jean Messon- Barreiro-Rosario, Adriana Barreiro-Rosario, et al.NEBNext® Ultra™ II Q5® Master Mix,NEB,M0544S Gibson Assembly Master Mix,NEB,E2611L XbaI,NEB,R0145S NcoI,NEB,R0193S RecBCD,NEB,M0345S AsiSI,NEB,R063L NEBNext Q5® High-Fidelity 2X Master Mix,NEB,M0492L Penicilin-Streptomycin,ThermoFisher,15140122 SDS,Sigma

(4) SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte ReprogrammingCancer Research CommunicationsSeptember 11, 2025Minwook Kim, Yoojeong Park, Rachel Covitz, Joseph Kwon, Jia-Jun Liu, Silvia Liu, Sungjin KoAddgene_179909) was digested with NotI-HF (New England Biolabs, R3189S) and NcoI (New England Biolabs, R0193S) to generate a linearized backbone. The coding regions of BMI1 and Sall4 were amplified from pT3-EH1α-BMI1

(5) Single-base polymorphism of the ACE2 and TNF-alpha genes in patients with the cardiac and indeterminate forms of Chagas diseaseBraz J Infect DisAugust 14, 2025Bruna da Cruz Carvalho, Cilmery Suemi Kurokawa, Rodrigo Mattos Dos Santos, Daniela Filadelfo Sanches, Simone Baldini Lucheis, Erika Alessandra Pellison Nunes da Costa0.5 μL of the restriction enzyme Nocardia corallina (NcoI) (New England BioLabs, Boston, MA, USA ‒ R0193L) were used. Digestion was performed at 37°C in a thermocycler for 2h, followed by 65°C for 10 min. The

(6) Engineering high-efficiency matriptase substrates using E. coli display for applications in prodrug activationCell Rep MethodsJune 16, 2025Mestre Borras A, Mehari H, Ståhl S, Löfblom J.protease,In-house produced,N/A 4-Aminophenylmercuric acetate,Sigma-Aldrich,164610 NcoI,New England Biolabs,R0193S SpeI,New England Biolabs,R3133S Streptavidin R-Phycoerythrin Conjugate (SAPE),Thermo Fisher Scientific,S866 Phusion

(7) A direct experimental test of Ohno’s hypothesisElifeApril 2, 2025Ljiljana Mihajlovic, Bharat Ravi Iyengar, Florian Baier, Içvara Barbier, Justyna Iwaszkiewicz, Vincent Zoete, Andreas Wagner, Yolanda Schaerlior kit,NdeI,New England Biolabs,NEB #R0111S, Commercial assay or kit,NcoI,New England Biolabs,NEB #R0193S, commercial assay or kit,DpnI,New England Biolabs,NEB #R0176S, "Chemical compound, drug",MnCl2,Sigma-Aldrich,Sigma

(8) Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cellsPLOS GeneticsDecember 23, 2024Eric Cheng, Ran Lu, Abigail R. GerholdL4440 vector were digested using ApaI (New England Biolabs, R0114S) and NcoI (New England Biolabs, R0193S) restriction enzymes. Ligation was performed using T4 DNA ligase (New England Biolabs, M0202S) and the

(9) Clinical, biochemical, and genetic study of TACE/TNF-α/ACE signaling pathway in pediatric COVID-19 infectionClinical and Experimental PediatricsDecember 15, 2024Ahmed El-Abd Ahmed, Sawsan M.A. Abuhamdah, Mohammed H. Hassan, Nagwan I. Rashwan, Eman A. Abd-Elmawgood, Haggagy Mansour, Hoda S. Sherkawy, Shymaa G. Rizkminutes at 72°C. The amplified product of the PCR was 107 bp. The restriction enzyme NcoI (Catalog No. R0193S, New England Biolabs) was used for digestion of the PCR products by incubation for 4 hours at 37°C.

(10) A large-scale cancer-specific protein–DNA interaction networkLife Science AllianceOctober 1, 2024Yunwei Lu, Anna Berenson, Ryan Lane, Isabelle Guelin, Zhaorong Li, Yilin Chen, Sakshi Shah, Meimei Yin, Luis Fernando Soto-Ugaldi, Ana Fiszbein, et al.Destination vectors were linearized using single-cutter restriction enzymes (R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L; New England Biolabs). Table S7. Gene promoter info. The pWM#2 and pWM#3 plasmids

(11) Cytochrome c prompts the recruitment of its nuclear partners SET/TAF-Iβ and NPM1 into biomolecular condensatesiScienceAugust 16, 2024Miguel Á. Casado-Combreras, Adrián Velázquez-Campoy, Marlène Martinho, Valérie Belle, Miguel A. De la Rosa, Irene Díaz-Moreno(Dithiothreitol),CalBioChem,Cat. n.: 3870-25GM NcoI (restriction enzyme),New England Biolabs,Cat. n.: R0193S BamHI-HF (restriction enzyme),New England Biolabs,Cat. n.: R3136S DpnI (restriction enzyme),Roche/Merck,Cat.

(12) SAS: Split antibiotic selection for identifying chaperones that improve protein solubilityHeliyonMarch 15, 2024Emily McNutt, Na Ke, Alexandre Thurman, James B. Eaglesham, Mehmet Berkmencloned into the pST7a vector. pST7a was cut with restriction enzymes NcoI (New England Biolabs, cat# R0193S) and XbaI (New England Biolabs, cat# R0145S), APH(7ʹʹ) was amplified using primers NK1036 and NK1041

(13) Chemoproteomic strategy identified p120-catenin glutathionylation regulates E-cadherin degradation and cell migrationCell Chemical BiologyDecember 1, 2023Dhanushika S. K. Kukulage, Maheeshi Yapa Yapa Abeywardana, Nadee N. J. Matarage Don, Ren-Ming Hu, Kyosuke Shishikura, Megan L. Matthews, Young-Hoon Ahn2020.11",N/A "2,2’- dithiodipyridine",Thermo Scientific,Cat# AAA1111806 NcoI,New England BioLabs,Cat# R0193S XhoI,New England BioLabs,Cat# R0146S T4 DNA ligase,New England BioLabs,Cat# M2200S IPTG,Goldbio,Cat#

(14) Doxycycline-inducible Expression of Proteins at Near-endogenous Levels in Mammalian Cells Using the Sleeping Beauty Transposon SystemBIO-PROTOCOLOctober 20, 2023Karolina Zak, Costin N. AntonescuD). Materials and reagents Restriction enzymes, e.g., NcoI and ClaI (New England Biolabs, catalog numbers: R0193L and R0197L, respectively)Competent DH5α E. coli (Thermo Fisher, catalog number: 18265017)D-MEM/F-12

(15) Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factorsNature CommunicationsOctober 18, 2023Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu, et al.linearized with single-cutter restriction enzymes (New England Biolabs R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L). The pWM#2 and pWM#3 plasmids for each promoter were integrated simultaneously

(16) Assembly dynamics of FtsZ and DamX during infection-related filamentation and division in uropathogenic E. coliNature CommunicationsDecember 1, 2022Bill SÃ¶derstrÃ¶m, Matthew J. Pittorino, Daniel O. Daley, Iain G. Dugginconstituently producing mEos3.2-DamX. Firstly, pGI4 was digested with NcoI and BamHI-HF (New England Biolabs, #R0193S and # R31136R, respectively). mEos3.2-DamX was amplified from pDD7 using Q5 DNA polymerase (New England

(17) Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastasesNature communicationsOctober 21, 2021Raman V, Van Dessel N, Hall CL, Wetherby VE, Whitney SA, Kolewe EL, Bloom SMK, Sharma A, Hardy JA, Bollen M, et al.(Invitrogen; catalog # V430-01). The PCR product was digested with NcoI, XhoI and DpnI (NEB, catalog #s R0193S, R0146S and R0176L). The PBAD-his-myc plasmid (Accession X81838) was digested with NcoI and XhoI and

(18) Neuronal activity-induced BRG1 phosphorylation regulates enhancer activationCell reportsJuly 13, 2021Kim B, Luo Y, Zhan X, Zhang Z, Shi X, Yi J, Xuan Z, Wu J.GE17–5280-01 SacI,New England Biolabs,Cat# R0156S BgIII,New England Biolabs,Cat# R0144S NcoI,New England Biolabs,Cat# R0193S T4 DNA ligase,New England Biolabs,Cat# M0202S ,, Critical commercial assays,Critical commercial assays,Critical

(19) Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin bindingStructure (London, England : 1993)June 3, 2021Khan N, Pelletier D, McAlear TS, Croteau N, Veyron S, Bayne AN, Black C, Ichikawa M, Khalifa AAZ, Chaaban S, et al.Proteins" "Restriction endonucleases: BamHI, NcoI, SalI, XhoI",New England Biolabs,"Cat: BamHI R0136S, NcoI, R0193S, SalI R0138S, XhoI R0146S" T4 DNA ligase,New England Biolabs,Cat: M0202T QuickChange II Site Directed

(20) In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPRNature metabolismMay 1, 2021Charbord J, Ren L, Sharma RB, Johansson A, Ågren R, Chu L, Tworus D, Schulz N, Charbord P, Stewart AF, et al.NLuc REV (ggccgccagtgtgatggatatccCGCCAGAATGCGTTCGCACA). The NLuc PCR product was digested with NcoI (R0193S, NEB) and BstxI (R0113S, NEB) restriction enzymes. Next, ligation of the NLuc insert was performed using

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NcoI

NcoI from New England Biolabs

Sequence

New England Biolabs

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NcoI from New England Biolabs

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