Nb.BbvCI from New England Biolabs

Product Specs
ItemNb.BbvCI
CompanyNew England Biolabs
Price $311.00 Inquire
Catalog NumberR0631L
Quantity5000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CCTCAGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.Lambda DNA,LUMICKS,Cat# 00001 Biotinylated DNA Hybrid DNA,LUMICKS,Cat# 00027 Nb.BbvCI,New England Biolabs,Cat# R0631S NheI-HF,New England Biolabs,Cat# R3131S BsrGI-HF,New England Biolabs,Cat# R3575S BsiWI-HF,New England Biolabs

(2) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce DempleB6003S) at 50°C for 1 h in a total reaction volume of 500 μl. Subsequently, 100 units of Nb.BbvCI (NEB, R0631L) were introduced into the reaction mixture and incubated at 37°C for 2 h. The nickases were then heat-inactivated

(3) CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus DiscoveryAdvanced ScienceNovember 1, 2025Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, et al.were then digested with 10 units of Nt.BspQI (NEB R0644S), Nt.BbvCI (NEB R0632S), or Nb.BbvCI (NEB R0631S) nicking enzymes in a 50 µL reaction with enzyme‐specific buffers (1× NEB r3.1 buffer for Nt.BspQI or

(4) A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactionsNucleic Acids ResJune 28, 2025Huijin Lee, Fahad Rashid, Jihee Hwang, James A London, Richard Fishel, James M Berger, Sua Myong, Taekjip Haexcess relative to the DNA molarity) were mixed with either Nt.BbvCI (NEB, R0632) or Nb.BbvCI (NEB, R0631) (1.75 μl, 17.5 units) and rCutSmart buffer (supplied with enzyme) (5 μl) in a total volume of 50 μl.

(5) Structural and molecular basis of PCNA-activated FAN1 nuclease function in DNA repairNat CommunMay 14, 2025F. Li, A. S. Phadte, M. Bhatia, S. Barndt, A. R. Monte Carlo Iii, C-F. D. Hou, R. Yang, S. Strock, A. Pluciennikpreviously45. 3′ (CAG)2 DNA substrates were generated from RCC DNAs using Nb.BbvCI (New England Biolabs, cat # R0631S). Recombinant human proliferating cell nuclear antigen (PCNA) was purified from E. coli harboring plasmid

(6) Post-assembly Plasmid Amplification for Increased Transformation Yields in E. coli and S. cerevisiaeChem & Bio EngineeringFebruary 27, 2025Thomas Fryer, Darian S. Wolff, Max D. Overath, Elena Schäfer, Andreas H. Laustsen, Timothy P. Jenkins, Carsten Andersenof RCA product was restricted by employing 10 U nicking endonuclease Nb.BbvCI (New England Biolabs, R0631L) in a 50 μL reaction volume with 1× rCutsmart buffer for 1 h at 37 °C, followed by a heat inactivation

(7) A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactionsbioRxivOctober 29, 2024Huijin Lee, Jihee Hwang, Fahad Rashid, James A. London, Richard Fishel, James M. Berger, Sua Myong, Taekjip Haexcess relative to the DNA molarity) were mixed with either Nt.BbvCI (NEB, R0632) or Nb.BbvCI (NEB, R0631) (1.75 μL, 17.5 units) and rCutSmart buffer (supplied with enzyme) (5 μL) in a total volume of 50 μL.

(8) Circular single-stranded DNA as switchable vector for gene expression in mammalian cellsNature CommunicationsOctober 20, 2023Linlin Tang, Zhijin Tian, Jin Cheng, Yijing Zhang, Yongxiu Song, Yan Liu, Jinghao Wang, Pengfei Zhang, Yonggang Ke, Friedrich C. Simmel, et al.digestion The recognition site sequence “5′-GC↓TGAGG-3′” for the nicking enzyme Nb.BbvCI (NEB, cat. no. R0631S) was inserted into the multiple clone sites of pl EGFP by using the traditional molecular cloning method.

(9) A method for generating user‐defined circular single‐stranded DNA from plasmid DNA using Golden Gate intramolecular ligationBiotechnology and BioengineeringOctober 1, 2023Isabell K. Strawn, Paul J. Steiner, Matilda S. Newton, Zachary T. Baumer, Timothy A. Whiteheadcomprised of the 20 μL cleanup step, 1.5 μL of nuclease free water, 10 U of either Nb.BbvCI (NEB; catalog # R0631L) or Nt.BbvCI (NEB; catalog # R0632L (1 μL of 10 units/μL), 10 U of Exonuclease III (1 μL of a 1:10 dilution

(10) Achieving single nucleotide sensitivity in direct hybridization genome imagingNature CommunicationsDecember 15, 2022Yanbo Wang, W. Taylor Cottle, Haobo Wang, Momcilo Gavrilov, Roger S. Zou, Minh-Tam Pham, Srinivasan Yegnasubramanian, Scott Bailey, Taekjip Hasame settings describe above. SSB-ddPCR against the CDKN2A gene was conducted as above with Nb.BbvCI (R0631S, NEB) nicking the bottom strand, an annealing temperature of 56 °C and HEX probes against the cut region

(11) Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking MutagenesisMethods in Molecular BiologyNovember 29, 2022Monica B. Kirby, Timothy A. Whitehead10 U/μL Nt.BbvCI (New England Biolabs Cat # R0632S/L). 10 U/μL Nb.BbvCI (New England Biolabs Cat # R0631S/L). 100 U/μL exonuclease III (New England Biolabs Cat # M0206S/L). 20 U/μL exonuclease I (New England Biolabs

(12) DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic functionElifeNovember 7, 2022Joshua Jeong, Joyce H Lee, Claudia C Carcamo, Matthew W Parker, James M Bergerenzymes, Cutsmart Buffer (NEB #B7204S) and BamHI-HF (NEB #R3136T, for linearization) or Nb.BbvCI (NEB #R0631S, for nicking) was added after a 5 min incubation period with ATP. Samples were then incubated at 37

(13) The active DNA-PK holoenzyme occupies a tensed state in a staggered synaptic complexStructureMay 1, 2021Morgan Hepburn, Daniel J. Saltzberg, Linda Lee, Shujuan Fang, Claire Atkinson, Natalie C.J. Strynadka, Andrej Sali, Susan P. Lees-Miller, David C. Schriemer2015)",N/A XhoI,New England Biolabs,Cat#R0146 BamHI,New England Biolabs,Cat#R0136 Nb.BbvVI,New England Biolabs,Cat#R0631S PreScission protease,GE Healthcare,Cat#27-0843-01 glow-discharged carbon film 400 mesh copper grid,Electron

(14) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. WalterM1404-10MG EDTA-free cOmplete protease inhibitor cocktail,Roche,Cat# 11873580001 Nb.BbvCI,NEB,Cat# R0631S Nt.BbvCI,NEB,Cat# R0632S Nt.BspQI,NEB,Cat# R0644S AflII,NEB,Cat# R0520S NcoI,NEB,Cat# R0193L MfeI-HF,NEB,Cat#

(15) Non-intertwined strands of plasmid DNA contradict the Watson and Crick model of DNA structureF1000ResearchNovember 10, 2020Pawan Kumar(Nb.BbvCI) or top (Nt.BbvCI) strand-specific nicking endonucleases (New England Biolabs, catalog no. R0631 and R0632] and exonuclease III (New England Biolabs, catalog no. M0206S) at 37°C for 30 min. Digested

(16) Non-intertwined strands of plasmid DNA contradicts the Watson and Crick model of DNA structureF1000ResearchOctober 1, 2020Pawan Kumar(Nb.BbvCI) or top (Nt.BbvCI) strand-specific nicking endonucleases (New England Biolabs, catalog no. R0631 and R0632] along with exonuclease III (New England Biolabs, catalog no. M0206S) at 37°C for 30 min.

(17) A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking MutagenesisBIO-PROTOCOLAugust 5, 2020Paul J Steiner, Zachary T Baumer, Timothy A Whitehead10.21769/BioProtoc.3697. www.bio-protocol.org/e3697 Bio-protocol 10(15): e3697. DOI:10.21769/BioProtoc.3697 14. Nb.BbvCI (New England BioLabs, catalog number: R0631S/L) 15. Exonuclease I (E. coli) (New England BioLabs, catalog number: M0293S/L) 16. Exonuclease III (E. coli

(18) Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seqMethods in Molecular BiologyJune 11, 2018Xiaoyun Xing, Bo Zhang, Daofeng Li, Ting Wang(Fisher Scientific; cat. no. FP2500060). Orange DNA loading dye (6×, Thermo Scientific; cat. no. R0631). 2-Log DNA ladder (NEB; cat. no. N3200L). Gel electrophoresis system (Thermo Scientific, Kalamazoo,

(19) Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg ExtractsMethods in EnzymologyJune 25, 2017Thomas G.W. Graham, Johannes C. Walter, Joseph J. Loparo6–8kDa MWCO) and clips 4°C microcentrifuge Nb.BbvCI restriction enzyme (New England Biolabs, Cat. #R0631S) T4 DNA ligase (New England Biolabs, #M0202M) Molecular biology grade agarose TBE buffer (see

(20) Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysisMethodsAugust 1, 2016Yeonee Seol, Marie-Paule Strub, Keir C. Neumanbetween positions 1093 and 1129 was similar to that for the hairpin DNA substrate except that Nb. BbvCI (R0631L, New England Biolabs) was used to nick the DNA rather than Nt. BbvCI. 2.4 RecQ helicase unwinding assay 2.4.1

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Nb.BbvCI

Nb.BbvCI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (20)

Reviews

Nb.BbvCI from New England Biolabs

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