Nt.BbvCI from New England Biolabs

Product Specs
ItemNt.BbvCI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0632S
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CC/TCAGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus DiscoveryAdvanced ScienceNovember 1, 2025Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, et al.Nb.BbvCI‐hairpin sequences were then digested with 10 units of Nt.BspQI (NEB R0644S), Nt.BbvCI (NEB R0632S), or Nb.BbvCI (NEB R0631S) nicking enzymes in a 50 µL reaction with enzyme‐specific buffers (1× NEB

(2) Autoregulation of the real-time kinetics of the human mitochondrial replicative helicaseNat CommunJuly 1, 2025Ismael Plaza-G A, María Ortiz-Rodríguez, Seth P. Buchanan, Samuel Miguez-Amil, Kateryna M. Lemishko, Fernando Moreno-Herrero, Rafael Fernandez-Leiro, Grzegorz L. Ciesielski, Borja Ibarrapositions 6389 and 6452 bp of one strand of the plasmid were nicked with endonuclease Nt.BbvCI (NEB #R0632S). The small DNA fragments between the nicked positions were thermally denatured by raising temperature

(3) A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactionsNucleic Acids ResJune 28, 2025Huijin Lee, Fahad Rashid, Jihee Hwang, James A London, Richard Fishel, James M Berger, Sua Myong, Taekjip Hasingle-stranded oligo (50-fold excess relative to the DNA molarity) were mixed with either Nt.BbvCI (NEB, R0632) or Nb.BbvCI (NEB, R0631) (1.75 μl, 17.5 units) and rCutSmart buffer (supplied with enzyme) (5 μl) in

(4) A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactionsbioRxivOctober 29, 2024Huijin Lee, Jihee Hwang, Fahad Rashid, James A. London, Richard Fishel, James M. Berger, Sua Myong, Taekjip Hasingle-stranded oligo (50-fold excess relative to the DNA molarity) were mixed with either Nt.BbvCI (NEB, R0632) or Nb.BbvCI (NEB, R0631) (1.75 μL, 17.5 units) and rCutSmart buffer (supplied with enzyme) (5 μL) in

(5) Mechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexesMolecular CellSeptember 19, 2024Rose Westhorpe, Johann J. Roske, Joseph T.P. Yeelesup, each containing 160 μg of maxiprep DNA, 1X CutSmart buffer (NEB #B6004) and 21 μl Nt.BbvCI (NEB #R0632) in a total volume of 400 μl for 2 hours at 37°C. A further 5 μl of Nt.BbvCI was added to each digestion

(6) The Atad5 RFC-like complex is the major unloader of proliferating cell nuclear antigen in Xenopus egg extractsJournal of Biological ChemistryJanuary 1, 2024Yoshitaka Kawasoe, Sakiko Shimokawa, Peter J. Gillespie, J. Julian Blow, Toshiki Tsurimoto, Tatsuro S. TakahashiTo make a PCNA loading site, a locus-specific nick was introduced by Nt.BbvCI (New England Biolabs, #R0632). The DNA was bound onto Sepharose beads at a ratio of 100 ng per 1 μl, following the procedure described

(7) A method for generating user‐defined circular single‐stranded DNA from plasmid DNA using Golden Gate intramolecular ligationBiotechnology and BioengineeringOctober 1, 2023Isabell K. Strawn, Paul J. Steiner, Matilda S. Newton, Zachary T. Baumer, Timothy A. Whiteheadof nuclease free water, 10 U of either Nb.BbvCI (NEB; catalog # R0631L) or Nt.BbvCI (NEB; catalog # R0632L (1 μL of 10 units/μL), 10 U of Exonuclease III (1 μL of a 1:10 dilution in 1X rCutSmart Buffer of 100

(8) Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAsiScienceApril 21, 2023Huo Xu, Fenglin Yang, Danlong Chen, Weilin Ye, Guohui Xue, Lee Jiapolymerase,New England Biolabs Inc.,M0212L Nt. BbvCI nicking endonuclease,New England Biolabs Inc.,R0632L H2O2,Aladdin,H112515 TMB,Aladdin,T274310 ,, Deposited data,Deposited data,Deposited data ,, Bulk

(9) Prime editing with genuine Cas9 nickases minimizes unwanted indelsNature CommunicationsMarch 30, 2023Jaesuk Lee, Kayeong Lim, Annie Kim, Young Geun Mok, Eugene Chung, Sung-Ik Cho, Ji Min Lee, Jin-Soo Kimand linear plasmid forms, 1 µg of supercoiled plasmid was incubated with 2 units of Nt.BbvCI (NEB, #R0632S) or SpeI (NEB, #R3133S) at 37 °C for 2 h. To produce ribonucleoprotein complexes, purified Cas9, nCas9

(10) The mechanism of replication stalling and recovery within repetitive DNANature CommunicationsDecember 1, 2022Corella S. Casas-Delucchi, Manuel Daza-Martin, Sophie L. Williams, Gideon Costerof interest (pGC504), 4 × 200 µg of the relevant plasmid was cut with 15 µl (150U) of Nt.BbvCI (NEB, R0632) in a 200 µl final volume reaction at 37 °C for 3 h. The reaction was stopped by adding 50 mM EDTA.

(11) Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking MutagenesisMethods in Molecular BiologyNovember 29, 2022Monica B. Kirby, Timothy A. WhiteheadT4 Polynucleotide Kinase (New England Biolabs Cat # M0201S/L). 10 U/μL Nt.BbvCI (New England Biolabs Cat # R0632S/L). 10 U/μL Nb.BbvCI (New England Biolabs Cat # R0631S/L). 100 U/μL exonuclease III (New England Biolabs

(12) Genotype-Phenotype Correlation and Functional Insights for Two Monoallelic TREX1 Missense Variants Affecting the Catalytic CoreGenes (Basel)June 30, 2022Giulia Amico et al.For the ‘[S] < Km’ experiments (see Figure 4), the dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 5 ng/µL (~0.83 nM), and the hT1 concentration was 7.5 nM. For the ‘[S] >> Km’

(13) Assembly of Longâ€Adapter Singleâ€Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA TargetsCurrent ProtocolsDecember 2, 2021Lamia Chkaiban, Lorenzo Tosi, Biju Parekkadan(New England BioLabs, cat. no. R0712S) Nt.BbvCI nicking endonuclease (New England BioLabs, cat. no. R0632S) Lambda Exonuclease (New England BioLabs, cat. no. M0262S) Exonuclease V (RecBCD) (New England BioLabs,

(14) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. WaltercOmplete protease inhibitor cocktail,Roche,Cat# 11873580001 Nb.BbvCI,NEB,Cat# R0631S Nt.BbvCI,NEB,Cat# R0632S Nt.BspQI,NEB,Cat# R0644S AflII,NEB,Cat# R0520S NcoI,NEB,Cat# R0193L MfeI-HF,NEB,Cat# R3589S "Klenow

(15) Non-intertwined strands of plasmid DNA contradict the Watson and Crick model of DNA structureF1000ResearchNovember 10, 2020Pawan Kumar(Nb.BbvCI) or top (Nt.BbvCI) strand-specific nicking endonucleases (New England Biolabs, catalog no. R0631 and R0632] and exonuclease III (New England Biolabs, catalog no. M0206S) at 37°C for 30 min. Digested plasmid

(16) Non-intertwined strands of plasmid DNA contradicts the Watson and Crick model of DNA structureF1000ResearchOctober 1, 2020Pawan Kumaror top (Nt.BbvCI) strand-specific nicking endonucleases (New England Biolabs, catalog no. R0631 and R0632] along with exonuclease III (New England Biolabs, catalog no. M0206S) at 37°C for 30 min. Digested plasmid

(17) A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking MutagenesisBIO-PROTOCOLAugust 5, 2020Paul J Steiner, Zachary T Baumer, Timothy A Whitehead(New England BioLabs, catalog number: M0201S/L) 13. Nt.BbvCI (New England BioLabs, catalog number: R0632S/L) Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC. 2 Please cite this article

(18) The mechanism of DNA unwinding by the eukaryotic replicative helicaseNature CommunicationsDecember 1, 2019Daniel R. Burnham, Hazal B. Kose, Rebecca B. Hoyle, Hasan Yardimcifollowed by PCR sample purification into 10 mM Tris-HCl, pH 8.5. After nicking with Nt.BbvCI (NEB, R0632S) at 37 °C for 3.5 hours, oligo 8 and 9 were added and heated at 50 °C for 10 min and again purified.

(19) Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicaseeLifeAugust 29, 2019Yeonee Seol, Gábor M Harami, Mihály Kovács, Keir C Neuman,T4 DNA ligase,Promega,Promega Cat. #: M1801, Chemical compound,Nt.BbvcI,New England Biolabs,NEB Cat. #: R0632, Chemical compound,BsaI-HF,New England Biolabs,NEB Cat. #: R3535, "Software, algorithm","LabVIEW,

(20) Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion ModelCell ReportsFebruary 19, 2019Hazal B. Kose, Nicolai B. Larsen, Julien P. Duxin, Hasan Yardimcinuclease,Sigma,E1014 HpaII Methyltranferase,NEB,M0214 T4 PNK,NEB,M0201 T4 Ligase,NEB,M0202 Nt.BbvCl,NEB,R0632 Nb.BsmI,NEB,R0706 HpaII Methyltransferase (M.HpaII),NEB,M0214 "Nunc 384 shallow well plate, black",Thermo

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Nt.BbvCI

Nt.BbvCI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (26)

Reviews

Nt.BbvCI from New England Biolabs

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