USEREnzyme from New England Biolabs

Product Specs
ItemUSEREnzyme
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberM5505S
Source/Expression SystemThe two component proteins are purified separately from E. coli K-12 strains containing plasmids encoding Endonuclease VIII and Uracil-DNA Glycosylase.
Quantity50 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to nick 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil base in a total reaction volume of 10 µl in 15 minutes at 37°C in 1X T4 DNA Ligase Buffer containing 10 pmol of flourescently labeled oligonucleotide duplex.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Targeting NLRP3 Inflammasome and Metabolic Dysregulation by Electroacupuncture: A Novel Therapeutic Strategy for Methamphetamine Withdrawal-Induced DepressionASN NeuroDecember 31, 2026Xiong Zhang, Xiao-Rui Zhao, Chun-Li You, Fang Zhang, Hong-Yi Zhu, Tian Gu, Jia LicDNA fragments were then end-repaired, polyadenylated, and ligated to Illumina adaptors. USER enzyme (M5505S, New England Biolabs) was used to selectively degrade the second strand, yielding a strand-specific

(2) Population discontinuity in the Paris Basin linked to evidence of the Neolithic declineNat Ecol EvolApril 3, 2026Frederik V. Seersholm, Abigail Ramsøe, Jialu Cao, Philippe Chambon, Karl-Göran Sjögren, Hugh McColl, Fabrice Demeter, Charleen Gaunitz, Lasse Vinner, Jesper Stenderup, et al.feasible, uracil-specific excision reagent (USER)-treated libraries were later constructed using the NEB M5505L enzyme (New England Biolabs) to remove deaminated cytosines and reduce the influence of post-mortem

(3) Dissecting host stress responses for predictable heterologous gene expression in E. coliNucleic Acids ResMarch 30, 2026Christoffer Rode, Felix Beulig, Mathias Jönsson, Morten H H Nørholm, Leonie J Jahn, Daniel C Zielinski, Bernhard O Palsson, Emre Özdemir, Lei Yangfragments were combined in a 10 µl USER reaction containing 1 µl USER Enzyme (New England Biolabs, M5505), 1 µl T4 DNA Ligase Buffer (New England Biolabs, B0202), and insert and backbone fragments at a 3:1

(4) Subsistence transition preceded population turnover in the eastern Colombian AndesbioRxivMarch 27, 2026Kendra Sirak, Miguel Delgado, Angélica Triana, Sebastián Rivas, Pedro Argüello, Ana María Boada, Javier Rivera-Sandoval, German Peña, Carl Langebaek, Juan Pablo Ospina, et al.large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer PE concentrate,QIAGEN,19065 1M Tris-HCl (pH8.0),Sigma Aldrich,AM9856 1M

(5) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.min with 1 ml 0.1× TE. Libraries were eluted from beads with 11 µl 1× TE and 1.5 µl USER enzyme (NEB, M5505S) for 15 min at 37°C, then again with 10.5 µl 1× TE and 1.5 µl USER enzyme (NEB, M5505S) for 15 min at

(6) A sequence motif for DNA double-strand break and telomere healing during programmed DNA eliminationbioRxivMarch 13, 2026Jansirani Srinivasan, Marro Agbaga, Vincent Terta, Brandon Estrem, James R. Simmons, Ryan Oldridge, Mehwish Iftikhar, Abigail West, Hannah Lam, Thomas C. Dockendorff, et al.sonication were repaired and A-tailed with END-seq adaptor 2. Hairpins were digested with USER (NEB, Cat# M5505), and the DNA was amplified with Illumina TruSeq primers and barcodes. The libraries were sequenced

(7) Papuan admixture predated the settlement of PalauCellMarch 10, 2026Yue-Chen Liu, Joanne Eakin, Jolie Liston, Rosalind Hunter-Anderson, Calvin Emesiochel, Kiblas Soaladaob, Sunny O. Ngirmang, Olivia Cheronet, Carla S. Hadden, Alexander Cherkinsky, et al.large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer PE concentrate,QIAGEN,19065 1M Tris-HCl (pH8.0),Sigma Aldrich,AM9856 1M

(8) Modeling age-related methylation changes in vitro with intestinal organoidsBMC BiologyMarch 6, 2026Himani Vaidya, Gennaro Calendo, Kelsey Keith, Anthony Pompetti, Curt Balch, Jozef Madzo, Jaroslav Jelinek, Jean-Pierre J. IssaT4 DNA ligase (NEB #M0202), at 16 °C overnight. Adapters were cleaved by incubation with USER enzyme (NEB #M5505), at 37 °C, for 15 min. Dual size selection with AMPure XP beads removed DNA fragments greater than

(9) Evidence that extra copies of chromosome 1q play a role in the early phases of pancreatic neoplasiaScience AdvancesFebruary 20, 2026Christopher Douville, Jeeun Parksong, Marco Dal Molin, Sarah Graham, Patricia T. Greipp, Ryan Knudson, Samuel Curtis, Yuxuan Wang, Lisa Dobbyn, Maria Popoli, et al.following critical modifications: (i) DNA was pretreated with 3 U of USER enzyme (New England Biolabs, M5505L) for 15 min at 37°C to excise uracil bases; (ii) the SPRI bead/PEG NaCl ratios used after each reaction

(10) Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organsCommunications BiologyFebruary 19, 2026Keita Fujiwara, Katsunori Fujiki, Tomoya O. Akama, Katsuhiko Shirahige, Ichiro Shiojima, Tomoyuki Nakamura, Maretoshi Hiraiuracil-containing forward primer and a biotinylated reverse primer, treated with USER enzyme (NEB, cat. M5505S), annealed to a ligation oligo, ligated with T4 ligase (NEB, cat. M0202S), and purified by ExoI digestion

(11) SAMSN1 restrains NK cell mediated anti-tumor immunity in hepatocellular carcinomaNature CommunicationsJanuary 21, 2026Ruifeng Wang, Huidi Chen, Hanyang Liu, Qiang Li, Guojing Yao, Fuling Li, Peng Sun, Tianli Dai, Jiabei Wang, Björn Nashan, et al.and Taq DNA ligase (NEB, Cat: M0208V). The tagmented DNA was then treated with USER enzyme (NEB, Cat: M5505S) and T4 polynucleotide kinase (NEB, Cat: M5505S), circularized intramolecularly with T4 DNA ligase (NEB,

(12) Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB InhibitionJ Exp Clin Cancer ResDecember 20, 2025Dan Liu, Runtian Li, Mingzhu Li, Ying Liang, Zhao Wang, Yang Sun, Pengling Geadapter ligation were performed, and the second cDNA strand was selectively digested using USER Enzyme (M5505S, NEB, USA) to generate strand-specific libraries. The libraries were PCR-amplified, purified, validated

(13) Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interferenceNucleic Acids ResDecember 11, 2025Jiulin Chan, Zhichao Wu, Mingli Liu, Tianming Wang, Hanyu Liu, Ruimeng Cao, Xiaolong Li, Xinwen Li, Siyao Zhan, Jiangbo Cheng, et al.and at 65°C for 30 min, followed by adaptor ligation at 20°C for 15 min. USER® Enzyme (3 μl, NEB, Cat#M5505L) was added, and the reaction was incubated at 37°C for 15 min. Beads were washed and resuspended in

(14) Paleogenomic evidence on the temporal continuity of indigenous goat exploitation in the Canary IslandsiScienceNovember 21, 2025Clara Díaz-Pérez, Jonathan Santana, Kevin G. Daly, Alejandra C. Ordóñez, Javier G. Serrano, Sara B. Armas-Quintana, Emilio Vacas-Fumero, Aitor Brito-Mayor, Simon-Pierre Gilson, Jacob Morales, et al.UltraPure™ Guanidine Hydrochloride,Invitrogen,Cat#15502016 Uracil Glycosylase Inhibitor,New England Biolabs,Cat#M0281L USER® Enzyme,New England Biolabs,Cat#M5505L KAPA HiFi HotStart ReadyMix,Roche,Cat#7958935001 ,, Critical commercial assays,Critical commercial

(15) Ancient genomes reveal basal Asian ancestries and dynamic population interactions over time on the southern Tibetan PlateauiScienceNovember 21, 2025Jingkun Ran, Yichen Liu, Shargan Wangdue, Xiaoyan Yang, Tianyi Wang, Yu Gao, Peng Cao, Yan Tong, Qingyan Dai, Songtao Chen, et al.mix,Fermentas,cat#R1121 "USER enzyme (mixture of Uracil-DNAglycosylase, UDG, and Endonuclease, EndoVIII)",NEB,cat#M5505L "Bst DNA Polymerase, Large Fragment",NEB,cat#M0275S "ATP, 10 mM stock solution",NEB,cat#9804 T4 Polynucleotide Kinase

(16) Long shared haplotypes identify the southern Urals as a primary source for the 10th-century HungariansCellOctober 16, 2025Balázs Gyuris, Leonid Vyazov, Attila Türk, Pavel Flegontov, Bea Szeifert, Péter Langó, Balázs Gusztáv Mende, Veronika Csáky, Andrey A. Chizhevskiy, Ilgizar R. Gazimzyanov, et al.large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer PE concentrate,QIAGEN,19065 1 M Tris-HCl pH 8.0,Sigma Aldrich,AM9856 1

(17) Detection of Human Brain Cancers using Genomic and Immune Cell Characterization of Cerebrospinal Fluid through CSF-BAMCancer DiscoveryOctober 6, 2025Alexander H. Pearlman, Yuxuan Wang, Anita Kalluri, Megan Parker, Joshua D. Cohen, Jonathan Dudley, Jordina Rincon-Torroella, Yuanxuan Xia, Ryan Gensler, Melanie Alfonzo Alfonzo Horowitz, et al.following critical modifications: 1) DNA was pretreated with 3 U of USER enzyme (New England BioLabs #M5505L) for 15 min at 37 °C to excise uracil bases; 2) the SPRI bead/PEG NaCl ratios used after each reaction

(18) Minimizing and quantifying uncertainty in AI-informed decisions: Applications in medicineProceedings of the National Academy of SciencesAugust 26, 2025Samuel D. Curtis, Sambit Panda, Adam Li, Haoyin Xu, Yuxin Bai, Itsuki Ogihara, Eliza O’Reilly, Yuxuan Wang, Lisa Dobbyn, Maria Popoli, et al.following critical modifications: 1) DNA was pretreated with 3 U of USER enzyme (New England BioLabs, M5505L) for 15 min at 37 °C to excise uracil bases; 2) the SPRI bead/PEG NaCl ratios used after each reaction

(19) Fragmentation signatures in cancer patients resemble those of patients with vascular or autoimmune diseasesProceedings of the National Academy of SciencesAugust 26, 2025Samuel D. Curtis, Tingshan Liu, Yuxin Bai, Yuxuan Wang, Sambit Panda, Adam Li, Haoyin Xu, Eliza O’Reilly, Lisa Dobbyn, Maria Popoli, et al.following critical modifications: 1) DNA was pretreated with 3 U of USER enzyme (New England BioLabs, M5505L) for 15 min at 37 °C to excise uracil bases; 2) the SPRI bead/PEG NaCl ratios used after each reaction

(20) The microRNA miR-243 directs poly(UG) modification of a somatic mRNA in C. elegansMicroPubl BiolAugust 13, 2025David D. Lowe, Martin Newman, Gary Ruvkun, Scott G. Kennedyand 20% PEG8000. After overnight incubation, Lambda Exonuclease (NEB, M0293L) and USER enzyme (NEB, M5505L) were added with Exo I buffer and incubated at 37 °C for 20 minutes. RNA was purified using the RNA

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USEREnzyme

USEREnzyme from New England Biolabs

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Specifications/Features

New England Biolabs

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Reviews

USEREnzyme from New England Biolabs

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