Automated FRAP Imaging System from FORMULATRIX

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Automated FRAP Imaging System

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Description

Fluorescence Recovery After Photobleaching

The automated FRAP imaging system was designed specifically for performing the FRAP assay on 96-well LCP crystallization setups. The entire system is automated and user-friendly requiring a few clicks of a button to initiate a complete analysis of your plate. All of the results and analyses are integrated into FORMULATRIX' protein crystallography specific software lab management software (ROCK MAKER and ROCK IMAGER).

Prior to setting up crystallization experiments, you can quickly screen Lipidic Cubic Phase (LCP) conditions using Fluorescence Recovery After Photobleaching (FRAP). FRAP is an optical technique that assesses how easily protein is moving about in an LCP drop. If protein is aggregated, or the LCP structure is collapsed, the protein will not diffuse and therefore never crystallize. FRAP is a great tool to accelerate crystallization methods and rule out suboptimal conditions without having to wait on the crystals actually growing.

FRAP comes in two configurations to better fit your lab's needs:
  • A benchtop model capable of imaging two plates at a time. Best for lab's where throughput is not a concern.
  • An integrated model that is built into a ROCK IMAGER 1000 which allows you to store and automatically image up to 970 plates. Two plates can be imaged at the same time - one with visible light and one with FRAP.

Features:
  • Prescreen for LCP Crystal Growth - Determine Protein Mobility High protein mobility and fast diffusion rates correlate well with crystallization conditions.
  • Save Time + Money - Rule Out Sub-Optimal Conditions Focus your research on those conditions conducive to LCP crystal growth.
  • Convenient + Easy-to-Use - Fully Automated Analysis Initiate a complete analysis of your plate with a few clicks and store all your data in one spot.