MseI from New England Biolabs

Product Specs
ItemMseI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0525S
Quantity500 units
Concentration4-20 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq)BIO-PROTOCOLJanuary 20, 2026Peng Chen, Shen Zhou, Haonan Wang, Jiaqi Gu, Yongquan Li, Yuxiao Changcartridge (BiOptic, catalog number: C105101) 13. MseI 10 U/μL (New England Biolabs, catalog number: R0525S) 14. MspI 20 U/μL (New England Biolabs, catalog number: R0106S) 15. AluI 10 U/μL (New England Biolabs,

(2) Attenuation of ATM signaling by ROS delays replicative senescence at physiological oxygenMolecular CellDecember 18, 2025Alexander J. Stuart, Kaori K. Takai, Railia R. Gabbasova, Henry Sanford, Ekaterina V. Vinogradova, Titia de Langeextraction of gDNA was not compatible with USTELA. DNA was digested overnight with MseI (60 U, NEB, R0525L) and NdeI (60U, NEB, R011S). Digested DNA (20 ng) was ligated to the 42-mer and 11-mer as described

(3) Efficient generation of germline chimeras in a non-rodent species using rabbit induced pluripotent stem cellsNat CommunJune 4, 2025Hong-Thu Pham, Florence Perold, Yannicke Pijoff, Nathalie Doerflinger, Sylvie Rival-Gervier, Maëlle Givelet, Anaïs Moulin, Manon Ressaire, Emilie Da Silva Fernandes, Valeska Bidault, et al.KEPi cells using the Qiamp DNA extraction kit (Qiagen, #51304) and digested overnight with MseI (NEB, #R0525S). The resulting DNA fragments were ligated overnight using T4 DNA ligase and 20 µM linkers [MseI linker

(4) Control of replication and gene expression by ADP-ribosylation of DNA in Mycobacterium tuberculosisThe EMBO JournalMay 8, 2025Rachel E Butler, Marion Schuller, Ritu Jaiswal, Jayanta Mukhopadhyay, Jim Barber, Suzie Hingley-Wilson, Emily Wasson, Alexassander Couto Alves, Ivan Ahel, Graham R Stewartmixture,Merck,77617 QuantiFluor® dsDNA System,Promega,E2671 Alu1,New England Biolabs,R0137 Mse1,New England Biolabs,R0525 Sal1,New England Biolabs,R0138 Zeta-Probe® Membrane,Bio-Rad,#1620158 Clarity Western ECL Substrate,Bio-Rad,#1705061 SuperSignal™

(5) SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular functionEMBO reportsNovember 1, 2024Ranjan G, Sehgal P, Scaria V, Sivasubbu S.Taq II (Tli RNase H Plus),Takara,RR82LR BglII,NEB,R0144S XhoI,NEB,R0146S Kpn1,NEB,R0142S MseI,NEB,R0525S Paraformaldehyde,Sigma-Aldrich,158127 DIG RNA Labeling Mix,Roche,11277073910 1x TrypLE Express,Gibco,12605010 Collagenase

(6) A model for accurate quantification of CRISPR effects in pooled FACS screensbioRxivJune 23, 2024Harold Pimentel, Jacob W. Freimer, Maya M. Arce, Christian M. Garrido, Alexander Marson, Jonathan K. Pritchard10 μL of ddPCR Supermix for Probes (Bio-Rad, Cat #1863024), 1 μL of MseI (New England Biolabs, Cat #R0525S), 1 μL of both the reference and target primer assays, and water to a total volume of 20 μL. A Bio-Rad

(7) Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibilityBiotechnology for Biofuels and BioproductsMay 31, 2024Aniket Anant Chaudhari, Anant Mohan Sharma, Lavi Rastogi, Bhagwat Prasad Dewangan, Raunak Sharma, Deepika Singh, Rajan Kumar Sah, Shouvik Das, Saikat Bhattacharjee, Ewa J. Mellerowicz, et al.gene using primers PCWL-58 and PCWL-59 (Additional file2: Table S1) followed by digestion with MseI (R0525S, New England Biolabs, UK). AnAXE1 gene was confirmed using primers PCWL-33 and PCWL-34 (Additional file2:

(8) Validation of Single‐Nucleotide Mosaic Variants Through Droplet Digital PCRCurrent ProtocolsMay 22, 2024Christopher M. Grochowski, Richard A. Gibbs, Harsha Doddapanenireaction: HaeIII (New England Biolabs, NEB, Catalogue Number: R0108S), MseI (NEB, Catalogue Number: R0525S), HindIII (NEB, Catalogue Number: R3104S), are recommended by Bio-Rad. Note: Each new assay design should

(9) The development of a custom RNA-sequencing panel for the identification of predictive and diagnostic biomarkers in gliomaJournal of Neuro-OncologyFebruary 16, 2024Yukina Shirai, Toshihide Ueno, Shinya Kojima, Hiroshi Ikeuchi, Rina Kitada, Takafumi Koyama, Fumiyuki Takahashi, Kazuhisa Takahashi, Koichi Ichimura, Akihiko Yoshida, et al.as the positive control. The reaction mixture was subjected to restriction enzyme treatment (MseI, R0525L, New England Biolabs, Ipswich, MA, USA), followed by droplet generation using an automated droplet generator

(10) Transgenic Tg(Kcnj10-ZsGreen) fluorescent reporter mice allow visualization of intermediate cells in the stria vascularisScientific ReportsFebruary 6, 2024Dillon Strepay, Rafal T. Olszewski, Sydney Nixon, Soumya Korrapati, Samuel Adadey, Andrew J. Griffith, Yijun Su, Jiamin Liu, Harshad Vishwasrao, Shoujun Gu, et al.ng of gDNA or H20 (negative controls), 2 units of MseI (New England Biolabs, Ipswich, MA, USA, Cat# R0525S, which had been diluted 1:5 in Diluent A, New England Biolabs, Cat# B8001S), 500 nM of each primer and

(11) Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damageNature CommunicationsJanuary 2, 2024Weifeng Zhang, Zhuo Yang, Wenjie Wang, Qianwen Sunimmunoprecipitation (DRIP) 5 μg of chloroplast DNA was fragmented with 5 U of DdeI (NEB, R0175V), MseI (NEB, R0525S), RsaI (NEB, R0167V), and AluI (NEB, R0137V) at 37 °C for 12 h. RNase H pretreated atrnh1c cpDNA was

(12) Social antagonism facilitates supergene expansion in antsCurrent BiologyDecember 1, 2023Giulia Scarparo, Marie Palanchon, Alan Brelsford, Jessica Purcellpeptides, and recombinant proteins" PstI,New England Biolabs,Cat# R0140L MseI,New England Biolabs,Cat# R0525L T4 DNA Ligase,New England Biolabs,Cat# M0202L Q5 Hot Start polymerase,New England Biolabs,Cat# M0493L ATP

(13) DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in ArabidopsisNature CommunicationsNovember 27, 2023Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen Sunaccording to references34,35. In brief, 1 μg fragmented DNA (digested by DdeI (R0175S, NEB), MseI (R0525S, NEB), NlaIII (R0125S, NEB) and MboI (R0147S, NEB) endonucleases) was used for S9.6 immunoprecipitation.

(14) HiCAR: Analysis of Open Chromatin Associated Long‐range Chromatin Interaction Using Low‐Input MaterialsCurrent ProtocolsOctober 11, 2023Xiaolin Wei, Duc Tran, Yarui DiaoTriton X-100 (Reagents and Solutions) 1.1X rCutsmart Buffer (Reagents and Solutions) MseI (NEB cat. no. R0525L) T4 DNA Ligase (NEB cat. no. M0202L) 20 mg/mL BSA (NEB cat. no. B9000S) 2X RCB Buffer (Reagents and

(15) Morphine Re-Arranges Chromatin Spatial Architecture of Primate Cortical NeuronsGenomics, Proteomics & BioinformaticsJune 1, 2023Liang Wang, Xiaojie Wang, Chunqi Liu, Wei Xu, Weihong Kuang, Qian Bu, Hongchun Li, Ying Zhao, Linhong Jiang, Yaxing Chen, et al.washed once with ice-cold PBS. Lastly, the cross-linked chromatin was digested with MseI (Catalog No. R0525L, NEB, Ipswich, MA) in NEB buffer for 6 h at 37 °C with simultaneous rotation at 15 r/min. MmeI half-linker

(16) A CRISPR/Cas9-generated mutation in the zebrafish orthologue of PPP2R3B causes idiopathic scoliosisScientific ReportsApril 26, 2023Marian Seda, Berta Crespo, Michelangelo Corcelli, Daniel P. Osborn, Dagan Jenkinssequencing, T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L). Splicing analysis of exon 2 was done using primers in exons 1 and 3 or exons 1 and 7, generating RT-PCR

(17) Achieving single nucleotide sensitivity in direct hybridization genome imagingNature CommunicationsDecember 15, 2022Yanbo Wang, W. Taylor Cottle, Haobo Wang, Momcilo Gavrilov, Roger S. Zou, Minh-Tam Pham, Srinivasan Yegnasubramanian, Scott Bailey, Taekjip HaSSB DNA. Another half of the genomic DNA (less than 8 ng/μL) was treated with 0.2 unit/μL MseI (NEB, R0525S) for 1 h at 37 °C, and MseI was deactivated by incubating the reaction 20 min at 65 °C. Following the

(18) Genetic variation in P-element dysgenic sterility is associated with double-strand break repair and alternative splicing of TE transcriptsPLOS GeneticsDecember 7, 2022Jyoti Lama, Satyam Srivastav, Sadia Tasnim, Donald Hubbard, Savana Hadjipanteli, Brittny R. Smith, Stuart J. Macdonald, Llewellyn Green, Erin S. Kelleheret al. [109]). Starting with 10-ng DNA, samples were first digested using MseI (New England Biolabs, R0525L). This enzyme has the restriction site T/TAA, cuts frequently along the genome, and unlike traditional

(19) STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cellsCell Rep MethodsOctober 24, 2022Blanch-Asensio A, Grandela C, Brandão KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, et al.,New England Biolabs,Cat#R0127S Esp3I,New England Biolabs,Cat#R0734S HaeIII,New England Biolabs,Cat#R0108S MseI,New England Biolabs,Cat#R0525S BpiI,ThermoFisher,Cat#ER1011 FastDigest Eco31I,ThermoFisher,Cat#FD0293 T4 DNA Ligase,New England Biolabs

(20) Decoding the spatial chromatin organization and dynamic epigenetic landscapes of macrophage cells during differentiation and immune activationNature communicationsOctober 4, 2022Lin D, Xu W, Hong P, Wu C, Zhang Z, Zhang S, Xing L, Yang B, Zhou W, Xiao Q, et al.ddH2O, 20 μl of 20% Triton X-100, 40 μl of 10× NEBuffer 2.1, and 30 μl of MseI (NEB, Catalog code: R0525L, 10 units/μl) was then added to the nuclei and incubated for 6 h at 37 °C with rotation at 15 r.p.m.

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MseI

MseI from New England Biolabs

Sequence

New England Biolabs

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MseI from New England Biolabs

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