MscI from New England Biolabs

Product Specs
ItemMscI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0534L
Concentration3-15 u/µL
Quantity1250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TGG/CCA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) doc2a and doc2b contribute to locomotor and social behaviors by down-regulating npas4b in zebrafishBMC BiologyJuly 1, 2025Yali Chi, Tao Feng, Zixin Du, Ping Huang, Wenjun Yu, Haihua Liu, Wanshan Wang, Xinping Yang, Liping HuangDNA was extracted from the injected embryos and subjected to PCR and digestion for genotyping. MscI (R0534, NEB, USA) and BseYI (R0635, NEB, USA) were used to digest the PCR products from doc2a and doc2b, respectively.

(2) Soluble RAGE enhances muscle regeneration after cryoinjury in aged and diseased micePloS oneJanuary 1, 2025Horwitz N, Florea M, Medha KC, Liu T, Garcia V, Kim R, Lam A, Messemer K, Rios C, Almada AE, et al.and cloned between the ITRs of the self-complementary backbone using NheI (NEB #R3131) and MscI (NEB #R0534) restriction enzyme sites. sRAGE coding sequence was then amplified from sRAGE transgenic animals with

(3) Detection of ribonucleotides embedded in DNA by Nanopore sequencingCommunications BiologyApril 23, 2024Lavinia Grasso, Adriano Fonzino, Caterina Manzari, Tommaso Leonardi, Ernesto Picardi, Carmela Gissi, Federico Lazzaro, Graziano Pesole, Marco Muzi-Falconicircular dsDNA molecules obtained were O/N digested at 25 °C with MscI (New England Biolabs catalogue # R0534) and SwaI (New England Biolabs catalogue # R0604) restriction enzymes in NEB buffer 3.1. Digestion products

(4) The adaptor protein 2 (AP2) complex modulates habituation and behavioral selection across multiple pathways and time windowsiScienceApril 19, 2024Rodrigo Zúñiga Mouret, Jordyn P. Greenbaum, Hannah M. Doll, Eliza M. Brody, Emma L. Iacobucci, Nicholas C. Roland, Roy C. Simamora, Ivan Ruiz, Rory Seymour, Leanne Ludwick, et al.proteins" ,, BsmAI Restriction Endonuclease,NEB,Cat# R0529S MscI Restriction Endonuclease,NEB,Cat# R0534S HinfI Restriction Endonuclease,NEB,Cat# R0155S I-SceI Restriction Endonuclease,NEB,Cat# R0694S Cas9

(5) A common allele increases endometrial Wnt4 expression, with antagonistic implications for pregnancy, reproductive cancers, and endometriosisNature CommunicationsFebruary 12, 2024Mihaela Pavličev, Caitlin E. McDonough-Goldstein, Andreja Moset Zupan, Lisa Muglia, Yueh-Chiang Hu, Fansheng Kong, Nagendra Monangi, Gülay Dagdas, Nina Zupančič, Jamie Maziarz, et al.restriction enzyme Tsp451 (NEB R0583), which cuts if the alternate T allele is present or with Msc1 (NEB R0534), which cuts if the C allele is present. Only clean bands were purified with QIAquick PCR purification

(6) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafishDevelopmental CellNovember 1, 2023Masahiro Shin, Hui-Min Yin, Yu-Huan Shih, Takayuki Nozaki, Daneal Portman, Benjamin Toles, Amy Kolb, Kevin Luk, Sumio Isogai, Kinji Ishida, et al.,New England Biolabs,R0132 BbsI-HF,New England Biolabs,R3539 BclI-HF,New England Biolabs,R3160 MscI,New England Biolabs,R0534 NsiI-HF,New England Biolabs,R3127 BsrGI-HF,New England Biolabs,R3575 BamHI-HF,New England Biolabs,R3136 SalI-HF,New England Biolabs

(7) Engineering yeast to induce the synthesis of GPI-APs with a permanent phosphoethanolamine on mannose 2 of the glycan moietySTAR ProtocolsSeptember 16, 2022Li Chen, David K. Banfieldmono-HCl,Sigma,Cat#L5626 L-methionine,Sigma,Cat#M9625 L-phenylalanine,Sigma,Cat#P2126 L-serine,Sigma,Cat#S4500 L-threonine,Sigma,Cat#T8625 L-tryptophan,Sigma,Cat#T0254 L-tyrosine,Sigma,Cat#T3754 L-valine,Sigma,Cat#V0500 Uracil,Sigma,Cat#U0750 MscI restriction endonuclease,New England Biolabs,Cat#R0534 Yeast Extract,Oxoid,Cat#LP0021 Peptone,Oxoid,Cat#LP0037 D-(+)- Glucose anhydrous,RDH,Cat#16325 D-(+)- Galactose

(8) Insight and Development of Advanced Recombinant Adeno-Associated Virus Analysis Tools Exploiting Single-Particle Quantification by Multidimensional Droplet Digital PCRHuman Gene TherapySeptember 1, 2022Jeanette Zanker, Sara Lázaro-Petri, Daniela Hüser, Regine Heilbronn, Adrien Savy0.1 U/μL of SmaI (New England Biolabs—no. RS0141S) or with 0.1 U/μL of MscI (New England Biolabs—no. R0534L) following the manufacturer's instructions. A total of 4 × 1011 vector genomes of rAAV1 were treated

(9) Establishment of centromere identity is dependent on nuclear spatial organizationCurrent BiologyJuly 25, 2022Weifang Wu, Toni McHugh, David A. Kelly, Alison L. Pidoux, Robin C. Allshire,New England Biolabs,Cat# R0146S SacI-HF,New England Biolabs,Cat# R3156S MscI,New England Biolabs,Cat# R0534S T4 DNA Ligase,New England Biolabs,Cat# M0202S PMSF,MERCK,CAS#329-98-6 Yeast Protease Inhibitor Cocktail,MERCK,P8215 IGEPAL

(10) Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortexSTAR ProtocolsJune 1, 2021Kenichiro Nagahama, Shuhei Fujino, Takaki Watanabe, Naofumi Uesaka, Masanobu KanoInc",CAT: # R0101 AgeI,"New England BioLabs, Inc",CAT: # R0552 MSCI,"New England BioLabs, Inc",CAT: # R0534 rAPid Alkaline Phosphatase,"Roche Molecular Systems, Inc",CAT: # 4898141001 ,, Biological samples,Biological

(11) Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease DigestionFrontiers in Plant ScienceApril 28, 2021Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S. PikaardBstZ17I-HF (NEB #R3594), SpeI-HF (NEB #R3133), BclI-HF (NEB #R3160), SnaBI (NEB #R0130), MscI (NEB #R0534), and PvuII-HF (NEB #R3151) at 37◦ C overnight. The buffer was then removed and replaced with 500 µL

(12) Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequencesPlant CommunicationsMarch 8, 2021Simon Sretenovic, Desuo Yin, Adam Levav, Jeremy D. Selengut, Stephen M. Mount, Yiping Qi109328) (Lowder et al., 2015) containing z-SpyCas9 was enzymatically digested with MscI (NEB, catalog no. R0534∗) and SacI (NEB, catalog no. R3156∗). Based on the synthesized gBlocks and digested vectors, the NEBuilder

(13) Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection SystemBio-protocolJanuary 20, 2021Landry LG, Mann SE, Anderson AM, Nakayama M.(NEB, catalog number: R0101S) f. XhoI (NEB, catalog number: R0146S) g. MscI (NEB, catalog number: R0534S) h. AleI-v2 (NEB, catalog number: R0685S) i. SalI (NEB, catalog number: R0138S) j. BglII (NEB,

(14) A Non-Synonymous Single Nucleotide Polymorphism in FASN Gene Alters FASN Enzyme Activity in Subcutaneous and Intramuscular Adipose Tissue in Holstein Friesian SteersAnnals of Animal ScienceJanuary 1, 2021David Cancino-Baier et al.period at 72°C for 5 min. The 759 bp amplicon obtained was then digested by the restrictase MscI (Cat. #R0534S, New England Biolabs, Massachusetts, USA) which included CutSmart buffer 1X, MscI restriction enzyme (3

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MscI

MscI from New England Biolabs

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MscI from New England Biolabs

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