Published customer image: Immunohistochemical staining of formalin fixed, paraffin embedded murine colonic tissue sections for zonulin-1 and occludin using Permanent aqueous mounting medium (BUF058) as a mountant.Image caption:Bl 7952 induces upregulation of zonulin-1 and occludin in colon. Mice were treated with Bl 7952 (n=10), with Bl 372 (n=8) or with PBS (n=10) on ten consecutive days or were left untreated (Naïve; n = 5). Colonic inflammation was induced by addition of 2.5% (w/v) DSS in the drinking water for 7 days. (A) Immunohistochemical detection of occludin and zonulin-1 proteins on representative paraffin-embedded sections of colon. (B) Representative western blotting of occludin and zonulin-1 proteins in the colonic mucosa. Expression of β-actin was used as internal control. (C) Evaluation of intestinal permeability by FITC-dextran. Serum levels of 4.0-kDa FITC-dextran were measured in naïve controls (n=5), PBS/DSS-treated (n=5) and Bl 7952/DSS-treated mice (n=5) 5 hours after its intragastric administration. Data are express as mean ± SEM. Unpaired Student's t-test was used for comparison of Bl 7952/DSS group vs. control PBS/DSS group (**p < 0.01).From: Srutkova D, Schwarzer M, Hudcovic T, Zakostelska Z, Drab V, Spanova A, et al. (2015)Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis i
PERMANENT AQUEOUS MOUNTING MEDIUM
Published customer image: Immunohistochemical staining of formalin fixed, paraffin embedded murine colonic tissue sections for zonulin-1 and occludin using Permanent aqueous mounting medium (BUF058) as a mountant.Image caption:Bl 7952 induces upregulation of zonulin-1 and occludin in colon. Mice were treated with Bl 7952 (n=10), with Bl 372 (n=8) or with PBS (n=10) on ten consecutive days or were left untreated (Naïve; n = 5). Colonic inflammation was induced by addition of 2.5% (w/v) DSS in the drinking water for 7 days. (A) Immunohistochemical detection of occludin and zonulin-1 proteins on representative paraffin-embedded sections of colon. (B) Representative western blotting of occludin and zonulin-1 proteins in the colonic mucosa. Expression of β-actin was used as internal control. (C) Evaluation of intestinal permeability by FITC-dextran. Serum levels of 4.0-kDa FITC-dextran were measured in naïve controls (n=5), PBS/DSS-treated (n=5) and Bl 7952/DSS-treated mice (n=5) 5 hours after its intragastric administration. Data are express as mean ± SEM. Unpaired Student's t-test was used for comparison of Bl 7952/DSS group vs. control PBS/DSS group (**p < 0.01).From: Srutkova D, Schwarzer M, Hudcovic T, Zakostelska Z, Drab V, Spanova A, et al. (2015)Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain
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