Description
CellPURE Biological Buffers
- Ultrapure zwitterionic biological buffers
- Optimized for cell/tissue culture work
- Wide applicability to molecular biology and biochemical studies
- Manufactured under strict quality control guidelines to ensure performance and reliabilty
The purpose of a buffer in a biological system is to maintain intracellular and extracellular pH within the physiological range and resist changes in pH due to the presence of internal and external influences. CellPURE Biological Buffers from Fisher BioReagents are ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Good and co-workers in 1966, these organic buffers have pKa values at or near physiological pH and minimal interference with biological processes. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477)
Advantages
- Cell culture tested
- Analyzed for the absence of nucleases and proteases
- Tested for endotoxin and bioburden levels
- pKa values mostly independent of temperature and concentration
- High water solubility
- Minimal permeability to biological membranes
Applications
- Tissue culture media and maintenance of cell lines
- Enzyme assays
- Electrophoresis of RNA
- Transfection of mammalian cells