RPMI-7951 from ATCC

Product Specs
ItemRPMI-7951
CompanyATCC
Price $1,995.00Supplier Page View Company Product Page
Catalog NumberHTB-66
TypeCell Biology
SpeciesHomo sapiens
TissueSkin
Cell LineRPMI-7951
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Skin
Age18 years
DiseaseMalignant Melanoma

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(21) Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer BiologyeLifeDecember 7, 2021Errington TM, Denis A, Allison AB, Araiza R, Aza-Blanc P, Bower LR, Campos J, Chu H, Denson S, Donham C, et al.summary of methodological details of results reported are described below. RPMI-7951 cells (ATCC, cat# HTB-66, RRID:CVCL_1666) and OUMS-23 cells (JCRB, cat# JCRB1022, RRID:CVCL_3088) were grown in RPMI (ATCC, cat#

(22) Investigation of Lupeol as Anti-Melanoma Agent: An In Vitro-In Ovo PerspectiveCurrent OncologyDecember 2, 2021Flavia Bociort, Ioana Gabriela Macasoi, Iasmina Marcovici, Andrei Motoc, Cristina Grosu, Iulia Pinzaru, Crina Petean, Stefana Avram, Cristina Adriana Deheleanevaluated on two human MM cell lines provided by ATCC as frozen vials—A375 (CRL-1619™), and RPMI-7951 (HTB-66™). The biosafety of LUP was assessed on two healthy skin cell types (human keratinocytes—HaCaT, and

(23) Cooperation between melanoma cell states promotes metastasis through heterotypic cluster formationDevelopmental cellOctober 25, 2021Campbell NR, Rao A, Hunter MV, Sznurkowska MK, Briker L, Zhang M, Baron M, Heilmann S, Deforet M, Kenny C, et al.HTB-72 A375,ATCC,Cat # CRL-1619 HS294T,ATCC,Cat # HTB-140 A2058,ATCC,Cat # CRL-11147 RPMI7951,ATCC,Cat # HTB-66 HMCB,ATCC,Cat # CRL-9607 HS895T,ATCC,Cat # CRL-7637 M000921,"(Raaijmakers et al., 2015)",N/A M111031,"(Raaijmakers

(24) Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma CellsToxicsSeptember 19, 2021Iulia Pinzaru, Raul Chioibas, Iasmina Marcovici, Dorina Coricovac, Razvan Susan, Denisa Predut, Doina Georgescu, Cristina Deheleanperformed on two human malignant melanoma cell lines provided from ATCC as frozen vials—RPMI-7951 (HTB-66™) and SK-MEL-28 (HTB-72™). The cell lines were cultured in their specific growth medium (EMEM) according

(25) Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancerCell reportsAugust 3, 2021Zaman A, Wu X, Lemoff A, Yadavalli S, Lee J, Wang C, Cooper J, McMillan EA, Yeaman C, Mirzaei H, et al.TBK1 −/− MEFs,"Ou et al., 2011",NA PKR wt and PKR−/−MEFs,Gift from Dr. John Bell,NA RPMI7951,ATCC,HTB-66 LOXIMVI,Millipore sigma,SCC201 YUMAC,Gift from Yale Skin Disease Research Center,NA LM20,Gift from

(26) Effects of Sponge-Derived Alkaloids on Activities of the Bacterial α-D-Galactosidase and Human Cancer Cell α-N-AcetylgalactosaminidaseBiomedicinesMay 5, 2021Natalia Utkina, Galina Likhatskaya, Olesya Malyarenko, Svetlana Ermakova, Larisa Balabanova, Lubov Slepchenko, Irina Bakunina350.1500 [M+H]+, (calcd. for C20H20N3O3: 350.1504). 2.5. Cell Cultured Cancer cells RPMI-7951 (ATCC® no. HTB-66™), MDA-MB-231 (ATCC® no.HTB-26™), DLD-1 (ATCC® no.CCL-221), HT-29 (ATCC® no.HTB-38™), HCT-116 (ATCC®

(27) Data-independent acquisition-based proteome and phosphoproteome profiling across six melanoma cell lines reveals determinants of proteotypesMolecular OmicsApril 5, 2021Erli Gao, Wenxue Li, Chongde Wu, Wenguang Shao, Yi Di, Yansheng Liucells (ATCC TCP-1013) were purchased from ATCC. The three metastatic cell lines include RPMI-7951 (ATCC HTB-66, named “7951” hereafter), SH-4 (ATCC CRL-7724, named “SH4”), and SK-MEL-3 (ATCC HTB-69, named “HTB69”).

(28) New Triterpene Glycosides from the Far Eastern Starfish Solaster pacificus and Their Biological ActivityBiomoleculesMarch 14, 2021Timofey V. Malyarenko, Alla A. Kicha, Anatoly I. Kalinovsky, Pavel S. Dmitrenok, Olesya S. Malyarenko, Alexandra S. Kuzmich, Valentin A. Stonik, Natalia V. IvanchinaCRL-1573™), colorectal carcinoma HT-29 cells (ATCC® no. HTB-38™), melanoma RPMI-7951 cells (ATCC® no. HTB-66), and breast cancer MDA-MB-231 cells (ATCC® HTB-26™) were obtained from the American Type Culture Collection

(29) Plasminogen Activator Inhibitor-1 Secretion by Autophagy Contributes to Melanoma Resistance to Chemotherapy through Tumor Microenvironment ModulationCancersMarch 12, 2021Tzeng HT, Yang JL, Tseng YJ, Lee CH, Chen WJ, Chyuan IT.melanoma cell line B16-F10 and the human melanoma cell lines A375 (ATCC® CRL-1619™) and RPMI-7951 (ATCC® HTB-66™) were purchased at the Bioresource Collection and Research Center (Hsinchu, Taiwan) and the American

(30) Suppression of p16 alleviates the senescence-associated secretory phenotypeAgingFebruary 6, 2021Buj R, Leon KE, Anguelov MA, Aird KM.Melanoma cell lines SKMel28, Hs 600.T, and RPMI-7951, were obtained from ATCC (HTB-72, CRL-7368, and HTB-66, respectively). SKMel28 and Hs 600.T as well as the lentiviral and retroviral packaging cells (293FT

(31) Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant MelanomaCell reportsOctober 20, 2020Maitituoheti M, Keung EZ, Tang M, Yan L, Alam H, Han G, Singh AK, Raman AT, Terranova C, Sarkar S, et al.HMEL-BRAFV600E,Gift from Dr. David Fisher’s laboratory,N/A Human: A375,ATCC,CRL-1619 Human: RPMI-7951,ATCC,HTB-66 Human: WM115,ATCC,CRL-1675 Human: WM266-4,ATCC,CRL-1676 Human: SKMEL-24,ATCC,HTB-71 Human: WM278,ATCC,CRL-2809 Mouse:

(32) Two New Alginate Lyases of PL7 and PL6 Families from Polysaccharide-Degrading Bacterium Formosa algae KMM 3553T: Structure, Properties, and Products AnalysisMarine DrugsFebruary 24, 2020Alexey Belik, Artem Silchenko, Olesya Malyarenko, Anton Rasin, Marina Kiseleva, Mikhail Kusaykin, Svetlana ErmakovaRussia). Human melanoma SK-MEL-5 (ATCC® no. HTB-70™), SK-MEL-28 (ATCC® no.HTB-72™), RPMI-7951 (ATCC® no. HTB-66™) cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). 4.9.2.

(33) In Vitro Anticancer and Proapoptotic Activities of Steroidal Glycosides from the Starfish Anthenea asperaMarine DrugsNovember 1, 2018Timofey V. Malyarenko, Olesya S. Malyarenko, Alla A. Kicha, Natalia V. Ivanchina, Anatoly I. Kalinovsky, Pavel S. Dmitrenok, Svetlana P. Ermakova, Valentin A. StonikUSA). 3.5.2. Cell Lines and Culture Conditions Human malignant melanoma RPMI-7951 cells (ATCC® no. HTB-66™), breast cancer T-47D cells (ATCC® no. HTB-133™), and colorectal carcinoma HT-29 cells (ATCC® no. HTB-38™)

(34) Integrative detection and analysis of structural variation in cancer genomesNature GeneticsOctober 1, 2018Jesse R. Dixon et al.Modified Eagle’s Medium supplemented with 10% FBS and antibiotics. SK-N-MC (ATCC HTB-10), RPMI-7951 (ATCC HTB-66) cells were cultured in Eagle’s Minimum Essential Medium supplemented with 10% FBS and antibiotics.

(35) NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27^Kip1expression in malignant melanoma and other human tumorsMolecular CarcinogenesisDecember 1, 2017Smitha Antony et al.L‐glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose) containing 10% FBS. RPMI‐7951 (#HTB‐66) and SK‐MEL‐24 (#HTB‐71) melanoma cell lines from ATCC were maintained in Eagle's Minimum Essential

(36) The Inhibitory Activity of Luzonicosides from the Starfish Echinaster luzonicus against Human Melanoma CellsMarine DrugsJuly 18, 2017Olesya S. Malyarenko, Sergey A. Dyshlovoy, Alla A. Kicha, Natalia V. Ivanchina, Timofey V. Malyarenko, Bokemeyer Carsten, von Amsberg Gunhild, Valentin A. Stonik, Svetlana P. ErmakovaCell Culture Culture 3.2. TM TM melanoma cell lines The SK-Mel-28 (ATCC ## HTB-72 HTB-72TM)) and RPMI-7951 (ATCC ## HTB-66 HTB-66TM)) melanoma were cultured as monolayer in DMEM medium, heat-inactivated FBS, FBS, medium, supplemented supplemented

(37) The Antitumor Antibiotics Complex of Aureolic Acids from the Marine Sediment-associated Strain of Streptomyces sp. KMM 9048Natural Product CommunicationsApril 1, 2017Nataliya I. Kalinovskaya, Lyudmila A. Romanenko, Anatoly I. Kalinovsky, Svetlana P. Ermakova, Pavel S. Dmitrenok, Shamil Sh. Afiyatullovwere purchased from Promega (Madison, WI). Cell culture: The SK-Mel-28 (ATCC # HTB-72) and RPMI-7951 (ATCC # HTB-66) human malignant melanoma cell lines were grown in monolayers in DMEM, supplemented with 10% (v/v) heat-inactivated

(38) Aphelasteroside F, a new Asterosaponin from the Far Eastern Starfish Aphelasterias japonicaNatural Product CommunicationsSeptember 1, 2016Roman S. Popov, Natalia V. Ivanchina, Anatoly I. Kalinovsky, Sofiya D. Kharchenko, Alla A. Kicha, Timofey V. Malyarenko, Svetlana P. Ermakova, Pavel S. Dmitrenok(Madison, WI). Cell culture: The SK-Mel-28 (ATCC # HTB-72), SK-Mel-5 (ATCC # HTB-70), and RPMI-7951 (ATCC # HTB-66) human malignant melanoma cell lines were grown in monolayers in DMEM, supplemented with 10% (v/v) heat-inactivated

(39) Registered Report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivationeLifeMarch 21, 2016Sharma V, Young L, Cavadas M, Owen K, Reproducibility Project: Cancer Biology..authors. A375 cells,Cell line,ATCC,CRL-1619,Original brand not specified. RPMI-7951 cells,Cell line,ATCC,HTB-66, OUMS-23 cells,Cell line,JCRB,JCRB1022,Original brand not specified. 6-well plates,Labware,Greiner

(40) New 6,6-Spiroketal from the Alga-Derived Fungus Penicillium LividumNatural Product CommunicationsFebruary 1, 2016Olesya I. Zhuravleva, Maria P. Sobolevskaya, Vladimir A. Denisenko, Natalya N. Kirichuk, Maxim E. Zhidkov, Svetlana P. Ermakova, Natalya Yu. Kim, Alekxandr S. Antonov, Elena V. Leshchenko, Shamil Sh. AfiyatullovC26H29O1179BrNa: 603.0836; found 603.0830. Cell culture: Human malignant melanoma RPMI-7951 (ATCC # HTB-66) and breast adenocarcinoma MDA-MB-231 (ATCC # HTB-26) cells were grown in monolayers in MEM and DMEM, respectively,

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RPMI-7951 from ATCC

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