BE(2)-M17 from ATCC

Product Specs
ItemBE(2)-M17
CompanyATCC
Price $551.00Supplier Page View Company Product Page
Catalog NumberCRL-2267
TypeCell Biology
SpeciesHomo sapiens
TissueBrain
Cell LineBE(2)-M17
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Brain
Cell Typeneuroblast
Age2 years
DiseaseNeuroblastoma
ApplicationsThis cell line is a suitable transfection host

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Lipid membrane remodeling by myristic acid treatment reverses Parkinson's disease α-synuclein phenotypes in patient neuronsNPJ Metab Health DisApril 14, 2026Julian Avila Pacheco, Gleidia Sauli, Luis Fonseca-Ornelas, Haley Cirka, Deepa Kumari, Morgan Hazo, Sarah Jeanfavre, Pedro H. Vendramini, Christina R. Muratore, Clary B. Clish, et al.interactions at synapses (Fig. S8). Methods Human neuroblastoma cells Human M17D neuroblastoma CRL-2267 (ATCC) cells were grown and maintained in DMEM 1x (Dulbecco’s Modification of Eagle’s Medium) with 4.5

(2) Phosphatidylserine Decarboxylase Regulates Retinal Ganglion Cell Neurite Outgrowth with Altered Somal Membrane Fluidics and Mitochondrial MorphologyBiomoleculesFebruary 9, 2026Sean D. Meehan, Sofia Yarosh, Victoria Pereira, Isabella Moceri, Sanjoy K. BhattacharyaBE2-M17 Neuroblastoma Cell Culture, Transfection, and Puromycin Selection BE2-M17 Neuroblastoma cells (CRL-2267 ATCC, Manassas, VA, USA) were maintained at 37 °C in a 5% CO2 Incubator with media consisting of a 1:1

(3) Jumonji domain-containing 6 promotes the expansion of neuroblastoma stem cells by activating the wingless/ integrated pathwayCytojournalJanuary 14, 2026Zuoqing Li, Wenzong Gao, Pengfei Gao, Yingchun Fei, Huadong Chen, Hong Jiang, Juntao Xie, Zhe Xumanufacturer’s instructions. The NB cells SKNDZ (cat. CRL-2149), SKNAS (cat. CRL-2137), BE2-M17 (cat. CRL-2267), SKNFI (cat. CRL-2142), IMR32 (cat. CCL-127), and SKNBE2 (cat. CRL-2271) were purchased from ATCC (Manassas,

(4) Toxoplasma gondii infection induces extracellular vesicle miRNAs in synaptic plasticity and neural mechanismsExtracellular Vesicles and Circulating Nucleic AcidsJanuary 9, 2026Poppy Cairney, Elton Rosas de de Vasconcelos, Glenn A. McConkeychanges. MATERIALS AND METHODS Cell culture and parasite propagation The neuroblast cell line BE(2)-M17 (ATCC CRL-2267) as described in our earlier work was used[15]. BE(2)-M17 cells were maintained in a 1:1 ratio of Optimem

(5) Dynamic redox monitoring in differentiated human neuroblastoma models of Parkinson's diseaseRedox RepDecember 2, 2025Jungeun Lim, Xavier Zhou, Srija Pamujula, Rukkia Liaqat, Ananya Achanta, Loren Looger, Benjamin C. Campbell, Melissa M. CampbellUSA) and 1% penicillin–streptomycin (Cat# 15140122; Fisher Scientific, USA). BE(2)-M17 cells (Cat# CRL-2267; ATCC, Manassas, VA, USA) were cultured in DMEM/F12 (Cat# 11330032, Fisher Scientific, Waltham, MA,

(6) Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biologyScience advancesJuly 18, 2025Mohan HM, Fernandez MG, Huang C, Lin R, Ryou JH, Seyfried D, Grotewold N, Barget AJ, Whiteley AM, Basrur V, et al.RRID:CVCL_0401" SK-N-BE(2)-M17(neuroblastoma),Gift from S. Barmada. Originally from ATCC.,"cat. no. CRL-2267, RRID:CVCL_0167" U-2 OS (osteosarcoma) G3BP1-GFP,Gift from S. Barmada. Figley et al. (69),N/A HEK293

(7) RILP cleavage links an inflammatory state to enhanced tau propagation in a cell culture model of Alzheimer's diseaseMolecular Biology of the CellMay 1, 2025Kayla E. King, Mohammad Haeri, Russell H. Swerdlow, Ann L. Wozniakcontrol (SHC001) were obtained from Sigma-Aldrich. Cell culture and transfection BE(2)-M17 cells (CRL-2267) were obtained from ATCC and cultured in DMEM/F12 10% FBS. HMC3 cells (CRL-3304) were purchased from

(8) 14-3-3θ phosphorylation exacerbates alpha-synuclein aggregation and toxicityNeurobiology of DiseaseMarch 1, 2025Bing Wang, Mary Gannon, Rudradip Pattanayak, Kasandra Scholz, Frank Sanders Pair, William J. Stone, Roschongporn Ekkatine, Zhongyu Liu, Talene A. Yacoubiantransduction of SK-N-BE(2)-M17 (M17) male neuroblastoma cells (obtained and authenticated by ATCC, #CRL-2267; RRID:CVCL_0167) with the doxycycline (doxy)-inducible αsyn pSLIK lentivirus, followed by selection

(9) TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanismsCell ReportsJanuary 28, 2025Megan M. Dykstra, Kaitlin Weskamp, Nicolás B. Gómez, Jacob Waksmacki, Elizabeth Tank, M. Rebecca Glineburg, Allison Snyder, Emile Pinarbasi, Michael Bekier, Xingli Li, et al.General Hospital",Ling et al.42 sTDP43-mNG HEK293T,This paper,N/A M17 human neuroblastoma cells,ATCC,CRL-2267 1021 + hNgn1/2 (iNeurons),Weskamp et al.19,- 1021 + hNgn2/Isl1/Lhx3 (iMotor neuron),Chua et al.52,- WTC11

(10) The gag-like gene RTL8 antagonizes PEG10-mediated virus like particlesPLOS ONEDecember 30, 2024Will Campodonico, Harihar M. Mohan, Phuoc T. Huynh, Holly H. Black, Cristina I. Lau, Henry L. Paulson, Lisa M. Sharkey, Alexandra M. Whiteleylines hTR-8/SVneo (CRL-3271), SK-N-SH (HTB-11), T98G (CRL-1690), CCF-STTG1 (CRL-1718), BE(2)-M17 (CRL-2267), and M059K (CRL-2365) cells were purchased from ATCC. A549 and U-87 MG cells were a gift from Dr. Roy

(11) SPLICER: a highly efficient base editing toolbox that enables in vivo therapeutic exon skippingNature CommunicationsNovember 28, 2024Angelo Miskalis, Shraddha Shirguppe, Jackson Winter, Gianna Elias, Devyani Swami, Ananthan Nambiar, Michelle Stilger, Wendy S. Woods, Nicholas Gosstola, Michael Gapinske, et al.serum and 1% penicillin/streptomycin at 37 °C with 5% CO2. BE(2)-M17 cells were obtained from the ATCC (CRL-2267) and maintained in 1:1 EMEM:Ham’s F12K supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin

(12) The BE (2)-M17 neuroblastoma cell line: revealing its potential as a cellular model for Parkinson’s diseaseFrontiers in Cellular NeuroscienceNovember 26, 2024Angel Carvajal-Oliveros, Camila Román-Martínez, Enrique Reynaud, Eduardo Martínez-Martínezderived from SK-N-BE (2), originally isolated from the brain tissue of a 2-year-old male in 1989 (ATCC, CRL-2267). This cell line expresses genes related to dopaminergic, adrenergic, and serotonergic pathways as well

(13) LINC00894 Regulates Cerebral Ischemia/Reperfusion Injury by Stabilizing EIF5 and Facilitating ATF4-Mediated Induction of FGF21 and ACOD1 ExpressionNeurochemical ResearchOctober 1, 2024Yifei Chen, Hengxiang Cui, Zhuanzhuan Han, Lei Xu, Lin Wang, Yuefei Zhang, Lijun LiuCell Culture Collection (ATCC) and cultured at 37 °C in a humidifier containing 5% CO2. Both BE(2)-M17 (CRL-2267) being simply referred to as M17 cells and SH-SY5Y (CRL-2266) cell lines were maintained in Opti-MEM

(14) Sequestosome-1 (SQSTM1/p62) as a target in dopamine catabolite-mediated cellular dyshomeostasisCell death & diseaseJune 18, 2024Masato A, Andolfo A, Favetta G, Bellini EN, Cogo S, Dalla Valle L, Boassa D, Greggio E, Plotegher N, Bubacco L.experiments. BE(2)-M17 cell lines maintenance, transfection, and treatments Neuroblastoma-derived BE(2)-M17 cells (ATCC CRL-2267) were previously authenticated and characterized [27]. Cells were cultured in 50% of Dulbecco’s modified Eagle’s medium (DMEM

(15) SARS-CoV-2 Spike Protein 1 Causes Aggregation of α-Synuclein via Microglia-Induced Inflammation and Production of Mitochondrial ROS: Potential Therapeutic Applications of MetforminBiomedicinesMay 31, 2024Moon Han Chang, Jung Hyun Park, Hye Kyung Lee, Ji Young Choi, Young Ho Kohwere imaged using a microscope (Olympus, Tokyo, Japan). 2.4. BE(2)-M17 Cell Culture BE(2)-M17 cells (CRL-2267) were purchased from ATCC (Manassas, VA, USA). Cells were grown in Dulbecco’s modified Eagle’s medium

(16) Sorghum Grain Polyphenolic Extracts Demonstrate Neuroprotective Effects Related to Alzheimer’s Disease in Cellular AssaysFoodsMay 30, 2024Nasim Rezaee, Eugene Hone, Hamid R. Sohrabi, Stuart Johnson, Leizhou Zhong, Prakhar Chatur, Stuart Gunzburg, Ralph N. Martins, W. M. A. D. Binosha FernandoBE (2)-M17 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (CRL-2267). Dulbecco’s modified Eagle’s medium–Ham’s nutrient mixture F-12 (DMEM/F12), fetal bovine serum (FBS)

(17) Adding hydrophobicity or positive charges to the cytosolic half of the α‐synuclein 3–11 helix increases membrane association and S129 phosphorylationFEBS LettersJanuary 1, 2024Kazuma Shimanaka, Bryan Shi, Lisa Brontesi, Heba Alnakhala, Vidyashree Jayanthi, Kanagaraj Subramanian, Nagendran Ramalingam, Arati Tripathi, Ulf Dettmerand transfection. Human neuroblastoma cells (BE (2)-M17, called M17D) were freshly acquired from ATCC (number CRL-2267] less than 3 years ago and had been authenticated by the vendor. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM

(18) Infection-induced extracellular vesicles evoke neuronal transcriptional and epigenetic changesScientific reportsApril 27, 2023Tedford E, Badya NB, Laing C, Asaoka N, Kaneko S, Filippi BM, McConkey GA.(GIBCO, USA) and 20 μg/ml−1 penicillin streptomycin (Sigma, USA). Human neuronal BE(2)-M17 cells (ATCC® CRL-2267) were maintained in a 1:1 ratio of F12 Hams and OptiMEM (GIBCO, USA) media supplemented with 10% horse

(19) The effects of KTKEGV repeat motif and intervening ATVA sequence on α-synuclein solubility and assemblyJournal of neurochemistryApril 1, 2023Brontesi L, Imberdis T, Ramalingam N, Dettmer U.line and transfection. Freshly acquired M17D human neuroblastoma cells (SK-N-BE(2)-M17, ATCC Cat# CRL-2267, RRID:CVCL_0167) were expanded, frozen in large quantities and not passaged beyond passage 15 (no authentication

(20) DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's diseaseNPJ Parkinson's diseaseMarch 25, 2023Masato A, Plotegher N, Terrin F, Sandre M, Faustini G, Thor A, Adams S, Berti G, Cogo S, De Lazzari F, et al.experiment. BE(2)-M17 cell line maintenance, transfection, and treatments Neuroblastoma-derived BE(2)-M17 cells (ATCC CRL-2267) were cultured in 50% of Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies) and 50% of F-12

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BE(2)-M17 from ATCC

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