293 c18 from ATCC

Product Specs
Item293 c18
CompanyATCC
Price $0.00Supplier Page View Company Product Page
Catalog NumberCRL-10852
TypeCell Biology
SpeciesHomo sapiens
Tissuekidney
Cell Line293 c18
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Kidney
Agefetus

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Altering the carbohydrate-binding specificity of the legume lectin FRIL through structure-guided engineeringNature CommunicationsMarch 5, 2026Yo-Min Liu, Hong Thuy Vy Nguyen, Xiaorui Chen, Md. Shahed-Al-Mahmud, Ting-Hua Chen, Kuo-Shiang Liao, Jennifer M. Lo, Tzu-Chun Kan, Chien-Tai Ren, Che MaPlasmids were transiently transfected into early log-phase (1.6 ~ 2.2 × 106 cells/ml) HEK293 EBNA (ATCC CRL-10852) or HEK293S (ATCC CRL-3022 GnTI-, for M5 HA) cells with PEI (polyethylenimine, linear, MW25,000, Polysciences

(2) Characterization of Usher Syndrome Type 2-Associated Proteins in the Retina via Affinity Purification-Mass SpectrometryMolecular & Cellular ProteomicsFebruary 5, 2026Junhuang Zou, Dongmei Yu, Pranav Dinesh Mathur, Cade Paul Nordhagen, Xinyue Zou, Paolo Bonaldo, Jun Yangunits/ml penicillin, and 100 μg/ml streptomycin (Thermo Fisher Scientific). The 293-EBNA cells (ATCC: CRL-10852; CVCL_6974) were maintained in DMEM F12 medium supplemented with 10% (v/v) fetal bovine serum, 100 units/ml

(3) Proteomics- and BRET- screens identify SPRY2 as a Ras effector that impacts its membrane organizationiScienceDecember 19, 2025Karolina Pavic, Fiona Elizabeth Hood, Carla Jane Duval, Ganesh babu Manoharan, Christina Laurini, Farid Ahmad Siddiqui, Stephanie Puy Lam Mo, Ian Andrew Prior, Daniel Kwaku Abankwathe HEK293-EBNA cell line gifted by Prof. Florian M. Wurm (EPFL Switzerland) or purchased from ATCC (CRL-10852). Cells were cultured in Dulbecco’s modified Eagle Medium (DMEM) (ThermoFisher Scientific, #41965039)

(4) Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progressionMol CancerDecember 18, 2025Erik Rasbach, Laure Migayron, Anne Brandenburg, Praveen Singh, Christina Martins, Ali Kassem, Anastasia I. Karkempetzaki, Nadine Suessner, Zsofi Kulcsar, Jessica Riopedre, et al.Addgene_41034). This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID: CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral

(5) Interdomain disulfide bonds of rabbit kappa light chain allotypes influence mouse-rabbit chimeric antibody performancebioRxivSeptember 23, 2025Yang-Nim Park, Douglas W. Houstonprior to transfection experiments. Recombinant antibody production Two cell lines, HEK293C18 (ATCC; CRL-10852, RRID:CVCL_6974) and Expi293F (Thermofisher, Catalog# A14635), were used for transient transfection

(6) Development and validation of a sensitive sandwich ELISA against human PINK1AutophagyFebruary 6, 2025Zahra Baninameh, Jens O. Watzlawik, Bernardo A. Bustillos, Gabriella Fiorino, Tingxiang Yan, Szymon L. Lewicki, Haonan Zhang, Dennis W. Dickson, Joanna Siuda, Zbigniew K. Wszolek, et al.and therapeutic biomarker. Materials and methods Cell culture, treatment and lysis HEK293E (ATCC, CRL-10852; originally marketed by Invitrogen) cells were cultured in DMEM (Thermo Fisher Scientific, 11965118)

(7) Biparatopic binding of ISB 1442 to CD38 in trans enables increased cell antibody density and increased avidityMAbsJanuary 30, 2025Jeremy Loyau, Thierry Monney, Marco Montefiori, Fedir Bokhovchuk, Jeremy Streuli, Matthew Blackburn, Arnaud Goepfert, Lydia N. Caro, Samitabh Chakraborti, Stefania De Angelis, et al.the OriP sequence to ensure episomal replication during production. HEK-EBNA cells (ATCC, Catalog NO: CRL-10852) were resuspended in RPMI1640 (Biowest, Catalog NO: L0501–500) and transfected with the previously mentioned

(8) Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variantsJournal of nanobiotechnologyJanuary 24, 2025Hwang J, Choi S, Kim BK, Son S, Yoon JH, Kim KW, Park W, Choo H, Kim S, Kim S, et al.plasmid was transfected using polyethlyenimine (PEI; Polysciences, Warrington, PA, USA) into HEK 293E (CRL-10852, ATCC) cells, and the transfected cells were cultured for seven days at 32 ℃ in 8% CO2 while being fed

(9) Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transferSTAR ProtocolsDecember 20, 2024Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwalines,Experimental models: Cell lines,Experimental models: Cell lines ,, "Human cell line, HEK293-EBNA (HEK)",ATCC,CRL-10852 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Human FNTA siRNA (Hs_FNTA_6 FlexiTube siRNA)

(10) The presence of broadly neutralizing anti-SARS-CoV-2 RBD antibodies elicited by primary series and booster dose of COVID-19 vaccinePLOS PathogensJune 10, 2024Xiaorui Chen, Arpita Mohapatra, Hong Thuy Vy Nguyen, Lisa Schimanski, Tiong Kit Tan, Pramila Rijal, Cheng-Pin Chen, Shu-Hsing Cheng, Wen-Hsin Lee, Yu-Chi Chou, et al.Spike protein sequence (14–1206, S2P) was cloned into pTT vector for expression in HEK293 EBNA (ATCC CRL-10852) suspension cells by transient transfection using FectoPRO (Polyplus) followed by 32°C incubation for

(11) Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myelomaNat CommunMarch 6, 2024C. Grandclément, C. Estoppey, E. Dheilly, M. Panagopoulou, T. Monney, C. Dreyfus, J. Loyau, V. Labanca, A. Drake, S. De Angelis, et al.produced in house by transient expression into suspension-adapted HEK293-EBNA cells (catalog no. ATCC-CRL-10852, LGC Standards, Teddington, UK) using polyethyleneimine. Briefly, equal quantities of each engineered

(12) Disruption of CFAP418 interaction with lipids causes widespread abnormal membrane-associated cellular processes in retinal degenerationsJCI InsightJanuary 9, 2024Anna M. Clark, Dongmei Yu, Grace Neiswanger, Daniel Zhu, Junhuang Zou, J. Alan Maschek, Thomas Burgoyne, Jun Yangat various time points. Littermate Cfap418+/+ or Cfap418+/– mice were included as controls. HEK293 (CRL-10852) and COS-7 (CRL-1651) cell lines were purchased from ATCC (https://www.atcc.org). These cells were cultured

(13) VWCE modulates amino acid-dependent mTOR signaling and coordinates with KICSTOR to recruit GATOR1 to the lysosomesNat CommunDecember 20, 2023Tianyu Zhao, Yuanyuan Guan, Chenchen Xu, Dong Wang, Jialiang Guan, Ying Liuimmunostaining) were from ThermoFisher. Cell lines and culture HEK293T (#CRL-3216) and HEK293E (#CRL-10852) were from ATCC, HepG2 was from Prof. Lei Chen (Peking University), Huh7 was from Prof. Xiao-Wei Chen

(14) Site‐specific photo‐crosslinking/cleavage for protein–protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cellsProtein ScienceDecember 1, 2023Kazue Terasawa, Tatsuro Seike, Kensaku Sakamoto, Kazumasa Ohtake, Tohru Terada, Takanori Iwata, Tetsuro Watabe, Shigeyuki Yokoyama, Miki Hara‐YokoyamaMETHODS 4.1 Cells and reagents The human embryonic kidney cell line (HEK293c18) was obtained from ATCC (CRL‐10852). HEK293c18 cells were suitable for vectors with the oriP sequence (Längle‐Rouault et al., 1998). The

(15) Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycansNature CommunicationsNovember 7, 2023David Chmielewski, Eric A. Wilson, Grigore Pintilie, Peng Zhao, Muyuan Chen, Michael F. Schmid, Graham Simmons, Lance Wells, Jing Jin, Abhishek Singharoy, et al.BHK21 (ATCC CCL-10) and adenovirus 5 DNA transformed human kidney epithelial cell line 293 c18 (ATCC CRL-10852) were maintained at 37 °C in a fully humidified atmosphere with 5% CO2 in DMEM (Gibco). All culture

(16) Mechanism-based inhibition of gut microbial tryptophanases reduces serum indoxyl sulfateCell Chemical BiologyNovember 1, 2023Amanda L. Graboski, Mark E. Kowalewski, Joshua B. Simpson, Xufeng Cao, Mary Ha, Jianan Zhang, William G. Walton, Daniel P. Flaherty, Matthew R. Redinbo8SL7 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines HEK293T,ATCC,CRL 10852 Caco-2,ATCC,HTB 37 Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(17) Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteomeThe Journal of biological chemistrySeptember 1, 2023Nůsková H, Cortizo FG, Schwenker LS, Sachsenheimer T, Diakonov EE, Tiebe M, Schneider M, Lohbeck J, Reid C, Kopp-Schneider A, et al.the function of individual proteins. Experimental procedures Cell culture HEK293E and HeLa cells (CRL-10852 and CCL-2, respectively; ATCC), were cultivated in high-glucose Dulbecco's modified Eagle's medium (Gibco)

(18) Universal features of Nsp1-mediated translational shutdown by coronavirusesbioRxivJune 13, 2023Katharina Schubert, Evangelos D. Karousis, Ivo Ban, Christopher P. Lapointe, Marc Leibundgut, Emilie Bäumlin, Eric Kummerant, Alain Scaiola, Tanja Schönhut, Jana Ziegelmüller, et al.μg/ml streptomycin (DMEM +/+) at 37°C, 5% CO2. For transient transfection of MERS-CoV, 293 c18 (ATCC® CRL-10852™), a human HEK293-EBNA cell line was used. Cells were cultured in EX-CELL 293 serum-free medium at 37°C,

(19) Substitution of PINK1 Gly411 modulates substrate receptivity and turnoverAutophagyJune 1, 2023Fiesel FC, Fričová D, Hayes CS, Coban MA, Hudec R, Bredenberg JM, Broadway BJ, Markham BN, Yan T, Boneski PK, et al.3xFLAG-PRKNC431S [28], parental and genetically modified human embryonic kidney cells HEK293E (ATCC, CRL-10852, originally marketed by Invitrogen) and HEK293T (ATCC, CRL-3216) were cultured in DMEM supplemented

(20) Sequence enrichment profiles enable target-agnostic antibody generation for a broad range of antigensCell Reports MethodsMay 22, 2023Jenny Mattsson, Anne Ljungars, Anders Carlsson, Carolin Svensson, Björn Nilsson, Mats Ohlin, Björn FrendéusDU 145,ATCC,Cat# HTB-81 "Human: Jurkat, Clone E6-1",ATCC,Cat# TIB-152 Human: HEK293 EBNA,ATCC,Cat# CRL-10852 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, "Forward MiSeq1: 5′-AATGATACGGCGACCA CCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTttccctgagactctcctgtgcagcctctggattcacctt-3′Upper

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293 c18 from ATCC

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