Fig 1: High-throughput multimodal imaging enables cell screening at a massive scale. (a) Montage of all 96 brightfield images captured by the 96 Eyes imager; (b) Magnified view of well A8 on the 96-well plate; (c) Low-resolution, out-of-focus raw image captured by the consumer-grade CMOS sensor; (d) Restored intensity of the object; (e) Restored phase; (f) Fluorescence response from eGFP; (g) Composite image of phase channel in gray color and eGFP channel in green color. The cells are S1P1-eGFP-Human bone osteosarcoma epithelial cells32 (U2OS; ATCC number HTB-96), seeded and fixed in the 96-well culture plate. Refer to Materials and methods for the detailed cell preparation protocol.