VK2/E6E7 from ATCC

Product Specs
ItemVK2/E6E7
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-2616
TypeCell Biology
SpeciesHomo sapiens
TissueVagina, Mucosa
Cell LineVK2/E6E7
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Vagina
Cell Typeepithelial cell
Age32 years
DiseaseEndometriosis
ApplicationsThese cell lines may provide the basis for valid, reproducible in vitro models for studies on cervicovaginal physiology and infections and for testing pharmacological agents for intravaginal application

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion AbilitiesOpen Forum Infect DisApril 25, 2026Masahiro Ito, Misaki Kataoka, Mio Aso, Nanae Netsu, Toshihiko Takada, Nanaka Okuda, Ayaka Sudo, Shiho Totsuka, Kirika Yoshioka, Yoichi Sato, et al.expressed as colony-forming units per milliliter (CFU/mL). Cell Culture Conditions VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture

(2) Vitamin D boosts HIV-1 resistance in female genital epithelial cells by enhancing antiviral cathelicidin expressionFront ImmunolApril 17, 2026Sandra M. Gonzalez, Wbeimar Aguilar-Jimenez, Maria T. Rugeles, Abu Bakar Siddik, Erica Hofer Labossiere, T. Blake Ball, Ruey-Chyi Suendocervical (End1) (ATCC CRL-2615™), ectocervical (Ect1) (ATCC CRL-2614™) and Vaginal (Vk2) (ATCC CRL-2616™) epithelial cells were used in this study. The cells were cultured in keratinocyte serum-free media

(3) Bacteriophage-mediated reduction of uropathogenic E. coli from the urogenital epitheliumInfection and ImmunityApril 15, 2026Bishnu Joshi, Jacob J. Zulk, Camille Serchejian, Zainab A. Hameed, Addison B. Larson, Austen L. Terwilliger, Deepak Kumar, Indira U. Mysorekar, Robert A. Britton, Anthony W. Maresso, et al.were maintained at −80°C in 20% glycerol. Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were grown in keratinocyte serum-free medium (KSFM) containing 50 mg/mL bovine pituitary extract and

(4) The role of L-type amino acid transporter 1 in the pathogenicity of Candida albicansMicrobiology SpectrumApril 7, 2026Yibing Lan, Cheng Wu, Peng Du, Jie Jiao, Chunming Li, Ketan Chu, Tao Zhang, Peiqiong Chen, An Li, Wenxian Xu, et al.germ-tube-forming cells was determined. Cell culture The human vaginal epithelial cell line VK2/E6E7 (ATCC CRL-2616) was used to study host-Candida interactions in vitro. Cells were cultured in keratinocyte serum-free

(5) Monkeypox virus replication and Host response in vaginal and ectocervical epithelial cellsVirulenceMarch 25, 2026Davide Mariotti, Ludovica Picarone, Alessandra D'Auria, Luigi Rosa, Valentina Valeriani, Daniele Pietrucci, Silvia Meschi, Fabrizio Carletti, Valentina Mazzotta, Guido Antonelli, et al.reagents Human Vaginal Epithelial Cells VK2/E6E7 and Human Ectocervical Epithelial Cells Ect1/E6E7 (CRL-2616 and CRL-2614 obtained from ATCC, Manassas, Virginia) were cultured in Keratinocyte Serum-Free Medium

(6) Polyethylene nano- and microplastics trigger metabolic stress responses in human vaginal epithelial cellsCell Death DiscoveryMarch 24, 2026Paola Pontecorvi, Matteo Cassandri, Alessandra Gianoncelli, Lorella Pascolo, Fabrizio Cece, Elena Niccolai, Simona Camero, Valentina Bonanni, Sara Bozzer, Enrico Romano, et al.in cell culture medium before experimental use. Cell Cultures and Treatments The VK2 E6/E7 cell line (ATCC CRL-2616), established from normal vaginal epithelial tissue of a premenopausal woman undergoing anterior–posterior

(7) Selective Human-Milk-Inspired Antimicrobial Peptides for the Treatment of Bacterial VaginosisPharmaceuticsMarch 17, 2026Ishita M. Shah, Carlito B. Lebrilla, J. Bruce German, David A. MillsUSA. 2.11. In Vitro Infection Assay and Impact on Inflammation VK2/E6 cells were obtained from ATCC (CRL-2616) and were propagated using the manufacturer’s instructions in DMEM with 10% FBS. For the infection assay,

(8) HIV-1 Vpr is targeted for degradation by autophagyPLoS PathogMarch 2, 2026Yuexuan Chen, Susanne Klute, Anju Bansal, Konstantin Maria Johannes Sparrer, Ruth Serra-Morenocomplete medium supplemented with 1 ng/mL of puromycin. VK2-E6-E7 vaginal epithelial cells (ATCC, #CRL 2616) were maintained in VK2 complete medium (Keratinoctye-SFM with BPE and EGF, Gibco, #10725–018) supplemented

(9) Exopolysaccharides of Lactobacillus crispatus mediate key balancing interactions with the vaginal mucosanpj Biofilms and MicrobiomesFebruary 19, 2026Vanessa Croatti, Caroline Dricot, Tom Eilers, Jelle Dillen, Tim Van Rillaer, Eline Cauwenberghs, Ilke Van Tente, Sam Bakelants, Dieter Vandenheuvel, Camille Allonsius, et al.condition was repeated three times. Cell lines and culturing The human vaginal VK2/E6E7 cell line (ATCC, CRL-2616) was maintained in Keratinocyte-serum free (KSFM-Gibco) medium supplemented with 0.1 ng/mL human recombinant

(10) D-lactic acid inhibits Candida albicans associated with vulvovaginal candidiasis: in vitro and in vivo effects and underlying mechanismsBMC MicrobiologyDecember 29, 2025Tingting Xia, Shujun Su, Rong Wu, Miaorong Zhan, Xuchu Hu, Huaiqiu Huangdesired concentration. Cell line and cell culture The VK2/E6E7 human vaginal epithelial cell line (ATCC CRL-2616), derived from the vaginal mucosa of a healthy premenopausal female, was used in this study. Cells were

(11) Cervicovaginal microbiome alters transcriptomic and chromatin accessibility signatures across cervicovaginal epithelial barriersMicrobiomeNovember 25, 2025Lauren Anton, Ana G. Cristancho, Briana Ferguson, Jacques Ravel, Michal A. ElovitzATCC# CRL-2614) (Ecto), endocervical (End1/E6E7, ATCC# CRL-2615) (Endo), and vaginal (VK2/E6E7, ATCC# CRL-2616) (VK2) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in

(12) Candida auris persists in the vaginal microaerobic niche in the absence of interleukin-17AmSphereOctober 29, 2025Masahiro Abe, Sota Sadamoto, Akiko Nagamori, Minoru Shinozaki, Sayoko Oiki, Amato Otani, Ami Koizumi, Takayuki Shinohara, Yoichiro Iwakura, Kazutoshi Shibuya, et al.assay to vagina epithelial cell line VK2/E6E7 VK2/E6E7, a human vaginal epithelial cell line (ATCC, CRL-2616), was cultured in keratinocyte serum-free medium (K-SFM; Invitrogen, CA, USA) on a 90 mm dish at 37°C

(13) Modulation of group B Streptococcus infection and vaginal cell inflammatory signaling in vitro by Lactobacillus crispatus-loaded electrospun fibersInfection and immunityOctober 14, 2025El-Baz N, Kyser A, Mahmoud MY, Farrell CZ, Ginocchio S, Frieboes HB, Doster RS.CFU. In vitro vaginal epithelial cell culture model Human vaginal epithelial VK2/E6E7 cells (ATCC CRL-2616) were cultured in keratinocyte-serum-free medium (KSFM, Gibco, Waltham, MA) supplemented with 0.1 ng/mL

(14) A Combination Native Outer Membrane Vesicle (NOVM) Vaccine to Prevent Meningococcal and Gonococcal DiseasePathogensSeptember 26, 2025Serena Giuntini, Scarlet W. Tefera, Alejandro Bolanos, Adan Ramos Rivera, Gregory R. Moeassays, monolayers of human cervical (ME-180, ATCC #HTP-33) and vaginal epithelial (VK2/E6E7, ATCC #CRL-2616) cells were incubated at 37 °C, 5% CO2 for four hours with ~1 × 105 CFU sialylated N. gonorrhoeae strains

(15) Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalisMolecular MicrobiologySeptember 23, 2025Rishi M. Ray, Phoebe V. Bridy, Aubree G. Musicant, Shiny Chandravel, Youstina Y. Aziz, Jasmine C. Cruz, Kimberly K. Jeffersonmaintained in expression cultures using 50 μg/mL chloramphenicol. 4.2 Tissue Culture VK2/E6E7 (ATCC CRL‐2616) cells were cultured at 37°C in 5% CO2 in complete Keratinocyte SFM supplemented with 1 IU mL−1 penicillin/streptomycin

(16) Mobiluncus mulieris alters the transcriptomic profile of cervicovaginal epithelial cells, shedding light on molecular drivers of adverse reproductive outcomesNPJ Biofilms MicrobiomesAugust 8, 2025Yu Hasegawa, Olivia Swain, Urvija Rajpal, Michael T. France, Liqhwa Ncube, Haocheng Yu, Ilaria Mogno, Amir Horowitz, Jacques Ravel, Michal A. ElovitzEct1/E6E7, Manassas, VA, USA), endocervical (End; ATCC CRL-2615, End1/E6E7), and vaginal (VK2; ATCC CRL-2616, VK2/E6E7) epithelial cell lines were cultured in keratinocyte-serum-free (KSF) media supplemented with

(17) 3-O Sulfated Heparan Sulfate (G2) Peptide Ligand Impairs the Infectivity of Chlamydia muridarumBiomoleculesJuly 12, 2025Weronika Hanusiak, Purva Khodke, Jocelyn Mayen, Kennedy Van, Ira Sigar, Balbina J. Plotkin, Amber Kaminski, James Elste, Bajarang Vasant Kumbhar, Vaibhav Tiwari(human epithelial cervix adenocarcinoma, ATCC CCL-2.1TM) and VK2/E6E7 (human vaginal epithelial; ATCC CRL-2616™) cell lines. The third cell line, i.e., Chinese hamster ovary (CHO-K1; ATCC CCL-61™), served as the

(18) Bacterial extracellular vesicles as a tunable platform for vaginal drug deliverybioRxivJune 11, 2025Darby Steinman, Varunaa Sri Hemanth Kumar, Ryan A McIlvaine, Pranshu Tyagi, Hahnbit Kang, Raifah Alam, Anguo Liu, Christopher M Jewell, Irina Burd, Sara Molinari, et al.Collection (ATCC; Manassas, VA). Human female reproductive tract cell lines Vaginal epithelial (VK2/E6E7, CRL-2616) and endocervical (End1/E6E7, CRL-2615) cells were sourced from American Type Culture Collection (ATCC;

(19) Identification and characterisation of vaginal bacteria-glycan interactions implicated in reproductive tract health and pregnancy outcomesNat CommunJune 5, 2025Virginia Tajadura-Ortega, Wengang Chai, Lauren A. Roberts, Yibing Zhang, Antonio Di Maio, Alexiane C. Decout, Benedita A. Pinheiro, Angelina S. Palma, Gian De Nicola, Lucia Riaposova, et al.bacterial overlay on glycan microarrays. Mammalian cell culture VK2/E6E7 vaginal epithelial cells (ATCC- CRL-2616) were cultured and maintained in Keratinocyte SFM 1X (Gibco) containing Calcium Chloride 0.4 mM and

(20) Drug screen reveals new potent host-targeted antivirals against Mpox virusResearchSquareJune 5, 2025Vaithilingaraja Arumugaswami, Arjit Vijey Jeyachandran, Nikhil Chakravarty, Nikhil Chakravarty, Yennifer Delgado, Yennifer Paravastu, Nivedha Satheeshkumar, Nivedha Satheeshkumar, Ephrem Jeysankar, Aakash Jeysankar, et al.and 1 % penicillin/streptomycin (P/S). The VK2/E6E7 (VK2) human vaginal epithelial cell line (ATCC, CRL-2616) was maintained in keratinocyte serum-free medium (KSFM) supplemented with 0.1 ng/mL human recombinant

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VK2/E6E7 from ATCC

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