AV3 from ATCC

Product Specs
ItemAV3
CompanyATCC
Price $399.00Supplier Page View Company Product Page
Catalog NumberCCL-21
SpeciesHomo sapiens
TypeCell Biology
Validated Species ReactivityHuman (Hu)
ApplicationsThis line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.The cells are positive for keratin by immunoperoxidase staining.NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

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Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Contemporary seasonal human coronaviruses display differences in cellular tropism compared to laboratory-adapted reference strainsJournal of VirologySeptember 23, 2025Matthew J. Gartner, Monique L. Smith, Clyde Dapat, Yi Wen Liaw, Thomas Tran, Randy Suryadinata, Joseph Chen, Guizhi Sun, Rory A. Shepherd, George Taiaroa, et al.easier and facilitate future research endeavors. MATERIALS AND METHODS Cell lines MRC-5 cells (CCL-21, ATCC) were maintained in DMEM supplemented with 50 U/mL penicillin, 50 µg/mL streptomycin, 1 mM sodium

(2) ME3BP-7 is a targeted cytotoxic agent that rapidly kills pancreatic cancer cells expressing high levels of monocarboxylate transporter MCT1ElifeMay 20, 2025Jordina Rincon-Torroella, Marco Dal Molin, Brian Mog, Gyuri Han, Evangeline Watson, Nicolas Wyhs, Shun Ishiyama, Taha Ahmedna, Il Minn, Nilofer S Azad, et al.CRL-1687, Cell line (H. sapiens),CFPAC-1,ATCC,Cat #CRL-1918, Cell line (H. sapiens),DLD-1,ATCC,Cat #CCL-21, Cell line (H. sapiens),MIA PaCa-2 MCT1 KO,This study,N/A, Cell line (H. sapiens),DLD-1 MCT1 KO,This

(3) Preclinical development of ozuriftamab vedotin (BA3021), a novel ROR2-specific conditionally active biologic antibody-drug conjugateMAbsApril 9, 2025Hwai Wen Chang, Gerhard Frey, Jing Wang, Haizhen Liu, Charles Xing, Jian Chen, William J. Boyle, Jay M. Shortcell line (ATCC, catalog no. HTB-70), and HT-1080, a human fibrosarcoma cell line (ATCC, catalog no. CCL-21), were cultured in MEM media (Gibco, catalog no. 11095-072) supplemented with 10% FBS. 293-F cells were

(4) The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranesEnvironmental Science and Pollution ResearchFebruary 24, 2025Coline Charnay, Audrey Antoine, Corinne Belville, Denis Gallot, Régine Minet-Quinard, Vincent Sapin, Loïc Blanchoncellular phenomena. Materials and methods In vitro culture The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for

(5) Unusual Vilasinin-Class Limonoids from Trichilia rubescensMoleculesJanuary 30, 2024Saidanxia Amuti, Yohei Saito, Shuichi Fukuyoshi, Katsunori Miyake, David J. Newman, Barry R. O’Keefe, Kuo-Hsiung Lee, Kyoko Nakagawa-GotoUniversity, New Haven, CT, USA). We confirmed our KB and KB-VIN are identical to AV-3 (ATCC number, CCL-21) as a HeLa (cervical carcinoma) derivative by short tandem repeat (STR) profiling. The antiproliferative

(6) Dysregulation of the Amniotic PPARγ Pathway by Phthalates: Modulation of the Anti-Inflammatory Activity of PPARγ in Human Fetal MembranesLife (Basel)April 6, 2022Audrey Antoine, Coraline De Sousa Do Outeiro, Coline Charnay, Corinne Belville, Fanny Henrioux, Denis Gallot, Loïc Blanchon, Régine Minet-Quinard, Vincent Sapinpurchased from Abliance (Compiègne, France). 2.2. Cell Culture The amniocyte epithelial cell line (AV3, CCL-21) was obtained from ATCC. AV3 was cultured under standard conditions (5% CO2, 95% humidified air, 37

(7) EVICAN—a balanced dataset for algorithm development in cell and nucleus segmentationBioinformaticsJune 1, 2020Mischa Schwendy, Ronald E Unger, Sapun H Parekh(ATCC) 4,Caco-2,Human,Colon,Colorectal adenocarcinoma,EMEM,20,a,HTB-37 (ATCC) 5,DLD-1,Human,Colon,Colorectal adenocarcinoma,RPMI,10,x,CCL-21 (ATCC) 6,HCT116,Human,Colon,Colorectal carcinoma,RPMI,10,x,CRL-247 (ATCC) 7,RKO,Human,Colon,Colon

(8) Antiproliferative Aspidosperma-Type Monoterpenoid Indole Alkaloids from Bousigonia mekongensis Inhibit Tubulin PolymerizationMoleculesMarch 31, 2019Yu Zhang, Masuo Goto, Akifumi Oda, Pei-Ling Hsu, Ling-Li Guo, Yan-Hui Fu, Susan L. Morris-Natschke, Ernest Hamel, Kuo-Hsiung Lee, Xiao-Jiang Haoshould be noted that we confirmed the KB and KB-VIN cell lines were identical to AV-3 (ATCC number, CCL-21) as a HeLa (cervical carcinoma) contaminant by short tandem repeat (STR) profiling. All cell lines were

(9) Kleinhospitine E and Cycloartane Triterpenoids from Kleinhovia hospitaJournal of Natural ProductsJuly 27, 2018Abdul Rahim, Yohei Saito, Katsunori Miyake, Masuo Goto, Chin-Ho Chen, Gemini Alam, Susan Morris-Natschke, Kuo-Hsiung Lee, Kyoko Nakagawa-Gotodescribed previously.27 We confirmed that the KB and KB-VIN cell lines were identical to AV-3 (ATCC number, CCL-21) as a HeLa (cervical carcinoma) contaminant by short tandem repeat profiling. All cell lines were obtained

(10) Production of Recombinant Chemokines and Validation of RefoldingMethods in EnzymologyFebruary 29, 2016Christopher T. Veldkamp et al.(Kar et al., 2011; Miller et al., 1992; Thomas et al., 2015). An example chemotaxis assay protocol for CCL21 is presented here. 2.4.1 Required materials for a general chemotaxis assay T2 cells (ATCC; 174 × CEM.T2

(11) The chemokine receptor CCR7 promotes mammary tumorigenesis through amplification of stem-like cellsOncogeneJanuary 7, 2016S T Boyle, W V Ingman, V Poltavets, J W Faulkner, R J Whitfield, S R McColl, M KochetkovaXTT Cell Proliferation Kit (ATCC) according to manufacturer’s instructions, using 100ng/ml CCL19 and CCL21. FCS was used at a concentration of 5%. 2 Supplementary References 1. Kochetkova M, Kumar S, McColl

(12) CCL21 Cancer ImmunotherapyCancers (Basel)May 7, 2014Yuan Lin, Sherven Sharma, Maie St. Johntumor in above two experiments may be explained by the different cancer cells used and the different CCL21 amount released in the study. B16F0 and B16F10 may not relate to each other, according to ATCC [16].

(13) Development of new folate-based PET radiotracers: preclinical evaluation of 68Ga-DOTA-folate conjugatesEuropean Journal of Nuclear Medicine and Molecular ImagingJanuary 1, 2011Melpomeni Fani, Xuejuan Wang, Guillaume Nicolas, Christelle Medina, Isabelle Raynal, Marc Port, Helmut R. Maeckecell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) (CCL-17 and CCL-21, respectively). The cells were Eur J Nucl Med Mol Imaging (2011) 38:108–119 Folate conjugates 111 Chemical

(14) The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesisMolecular CancerDecember 1, 2010Sébastien Bergeron, Etienne Lemieux, Véronique Durand, Sébastien Cagnol, Julie C Carrier, Jacques G Lussier, Marie-Josée Boucher, Nathalie Rivardmedium containing 10% FCS and in DMEM containing 10% FBS. The colon adenocarcinoma cell lines DLD-1 (CCL-21) (kindly obtained from Dr F. Boudreau, Université de Sherbrooke, QC, Canada) and Colo205 (CCL-222, ATCC)

(15) Genetic perturbation of the putative cytoplasmic membrane-proximal salt bridge aberrantly activates alpha(4) integrinsBloodDecember 15, 2008Yoichi Imai, Eun Jeong Park, Dan Peer, António Peixoto, Guiying Cheng, Ulrich H. von Andrian, Christopher V. Carman, Motomu Shimaokahybridomas (ATCC). Murine MAdCAM-1-, VCAM-1and ICAM-1-Fc fusion proteins, fibronectin, TNF-α, CXCL12, and CCL21 were acquired from R&D Systems. CalceinAM, CFSE (Carboxyfluorescein diacetate, succinimidyl ester) and

(16) Regulatory Role of Lymphoid Chemokine CCL19 and CCL21 in the Control of Allergic RhinitisThe Journal of ImmunologyNovember 1, 2007Kaoru Takamura, Satoshi Fukuyama, Takahiro Nagatake, Dong-Young Kim, Aya Kawamura, Hideyuki Kawauchi, Hiroshi KiyonoPBS-treated; O, OVA-treated. using an EndoFree Plasmid Maxi kit (Qiagen). For the detection of CCL19/ CCL21 expression, each plasmid was transfected into COS-7 (CRL-1651; ATCC) in Opti-MEM (Invitrogen) by electrophoresis

(17) Targeting tumor-associated fibroblasts improves cancer chemotherapy by increasing intratumoral drug uptakeJournal of Clinical InvestigationJuly 3, 2006Markus Loeffler, Jörg A Krüger, Andreas G Niethammer, Ralph A ReisfeldDepartment of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA), IL-2 (ATCC), and CCL21 (InvivoGen) were subcloned into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). For transient transfections,

(18) Synergistic antitumor effect by coexpression of chemokine CCL21/SLC and costimulatory molecule LIGHTCancer Gene TherapyApril 1, 2004Masayuki Hisada, Takayuki Yoshimoto, Sadahiro Kamiya, Yasushi Magami, Hiroko Miyaji, Toshihiko Yoneto, Koji Tamada, Tatuya Aoki, Yasuhisa Koyanagi, Junichiro Mizuguchisodium azide. Slides were incubated with Cancer Gene Therapy 281 Synergistic antitumor effect by CCL21 and LIGHT M Hisada et al 282 2% normal rat serum and 1% BSA and then sequentially with biotinylated

(19) Overexpression of CCL-21/secondary lymphoid tissue chemokine in human dendritic cells augments chemotactic activities for lymphocytes and antigen presenting cellsMolecular CancerNovember 2, 2003Karen Riedl, Felicita Baratelli, Raj K Batra, Seok Chul Yang, Jie Luo, Brian Escuadro, Robert Figlin, Robert Strieter, Sherven Sharma, Steven Dubinettadenovirus (AdCCL21) following homologous recombination. The control vector (AdCV) did not contain the CCL-21 cDNA insert. Clones of AdCCL-21 and AdCV were obtained by limiting dilution analysis of the ability

(20) Single histidine residue in head-group region is sufficient to impart remarkable gene transfection properties to cationic lipids: evidence for histidine-mediated membrane fusion at acidic pHGene TherapyAugust 1, 2003V V Kumar, C Pichon, M Refregiers, B Guerin, P Midoux, A Chaudhurihepatocarcinoma HepG2 cells (8055 HB, ATCC, Rockville, MD, USA), human epithelial ovary carcinoma HeLa cells (CCL21, ATCC), human carcinoma lung A549 cells (CCL185, ATCC) and human embryo kidney 293T7 cells25 were cultured

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AV3 from ATCC

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