FHs 74 Int from ATCC

Product Specs
ItemFHs 74 Int
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCCL-241
TypeCell Biology
SpeciesHomo sapiens
TissueSmall intestine
Cell LineFHs 74 Int
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Small Intestine
Cell Typeenterocyte
Age3 to 4 months gestation
DiseaseNormal
ApplicationsThis non-malignant cell line can be used for absorption, transport, and secretion studies in the Gastrointestinal (GI) Barrier Model

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathwayEuropean Journal of Medical ResearchDecember 2, 2025Xiaolong Wu, Jialing Wu, Junmin Xu, Biqin Chen, Jintao Jiang, Xurui Sun, Zengjie ChiCrohn’s disease. Material and methods Cell culture Human small intestinal epithelial cell line FHs74Int (CCL-241, ATCC, Manassas, VA) was STR-authenticated by STR profiling (all core loci matching; match probability

(2) Selective Targeting of Senescent FHs74Int Cells by Human Breast Milk Free Fatty AcidsBiology (Basel)October 3, 2025Tony Tremblay, Lionel LoubakiCell Culture The FHs74 Int cells (FHs74) were obtained from the American Type Culture Collection (ATCC; CCL-241; Manassas, VA, USA). These cells were extracted from the small intestine and thus are composed of a

(3) Global transcriptional analysis of human FHs 74 Int intestinal epithelial cells after exposure to advanced glycation end productsPLOS OneSeptember 10, 2025Katharina Schwertner, José Basílio, Karin Hoffmann-Sommergruber, Isabella Ellinger, Sabine Geiselhartepithelial cells Human FHs 74 Int cells were obtained from the American Type Culture Collection (ATCC, CCL-241) and maintained following ATCC instructions. Briefly, cells were grown in T75 flasks (surface activated

(4) 15-Lipoxygenase-dependent radiomitigation by NO●-Donors suppresses ferroptosis in intestinal Epithelium: Multiomics MS imaging and LC-MS evidenceRedox BiolJuly 21, 2025Yulia Y. Tyurina, Hua Tian, Haider H. Dar, Mert Akdogan, Ecem Saritas, Vladimir A. Tyurin, Louis J. Sparvero, Alexander A. Kapralov, Galina Shurin, Renee Fisher, et al.selection and targeted specific delivery. 4 Materials and methods Human intestine cells FHs 74 Int (ATCC, CCL-241), Hybri-care media (ATCC, 46-X), RSL3 (Selleck Chemicals, S8155), 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine

(5) Toxoplasma gondii modulates the host cell cycle, chromosome segregation, and cytokinesis irrespective of cell type or species originParasites & vectorsApril 5, 2024Rojas-Baron L, Senk K, Hermosilla C, Taubert A, Velásquez ZD.donor,PromoCell,C-12200,469Z003.1 HUVEC-p single donor,PromoCell,C-12200,473Z022.1 HFF-1,ATCC,SCRC-1041, FHs74,ATCC,CCL-241, Primary cells used in the current study

(6) Decreased expression of the NLRP6 inflammasome is associated with increased intestinal permeability and inflammation in obesity with type 2 diabetesCellular and molecular life sciences : CMLSFebruary 5, 2024Frühbeck G, Gómez-Ambrosi J, Ramírez B, Becerril S, Rodríguez A, Mentxaka A, Valentí V, Moncada R, Reina G, Baixauli J, et al.to evaluate the effects of the adipocyte secretome on the expression of inflammasome components in CCL-241, a non-cancerous small intestine cell line. CCL-241 cell line was obtained from the ATCC® (Middlesex,

(7) Heating and storage of structured acylglycerols with succinyl-linked stigmasterol residue does not cause negative chemical or biological changesSci RepDecember 4, 2023M. Rudzińska, A. Grygier, A. Olejnik, K. Kowalska, D. Kmiecik, A. Chojnacka, W. Gładkowski, A. Grudniewska, R. Przybylskidetermined in normal human cells from the digestive system, including the small intestine FHs 74 Int (ATCC CCL241), colon mucosa CCD 841CoN (ATCC CRL-179), and liver epithelial THLE-2 (ATCC CRL-2706) cells obtained

(8) Elevated expression of histone deacetylase HDAC8 suppresses arginine-proline metabolism in necrotizing enterocolitisiScienceJune 16, 2023Guo T, Hu S, Xu W, Zhou J, Chen F, Gao T, Qu W, Chen F, Lv Z, Lu L.Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, FHs74Int,ATCC,"Cat# CCL-241, RRID: CVCL_2899" IEC6,ATCC,"Cat# CRL-1592, RRID: CVCL_0343" ,, Experimental models,Experimental models

(9) Epithelial-derived galectin-9 containing exosomes contribute to the immunomodulatory effects promoted by 2’-fucosyllactose and short-chain galacto- and long-chain fructo-oligosaccharidesFrontiers in ImmunologyDecember 22, 2022Veronica Ayechu-Muruzabal, Merel de Boer, Bart Blokhuis, Alinda J. Berends, Johan Garssen, Aletta D. Kraneveld, Belinda van’t Land, Linette E. M. Willemsenintestinal mucosa. 2 Material and methods 2.1 Intestinal epithelial cells Human FHs 74 Int (ATCC, CCL-241™) and human HT-29 (ATCC, HTB-38™) cell lines were used as models for IEC. FHs 74 Int were grown in T25

(10) Uncovering the antimalarial potential of toad venoms through a bioassay-guided fractionation processInternational Journal for Parasitology: Drugs and Drug ResistanceDecember 1, 2022Mathilde Wells, Mathieu Fossépré, Stéphanie Hambye, Mathieu Surin, Bertrand Blankertevaluation 2.8.1 Cell lines and cultures FHs74int cells (human fetal intestinal epithelial cells, ATCC # CCL-241, American Type Culture Collection, St. Cloud, MN, USA) were cultured in Hybri-Care medium (46-X, American

(11) Bis-aryl-α,β-unsaturated ketone (ABK) chaperonin inhibitors exhibit selective cytotoxicity to colorectal cancer cells that correlates with levels of aberrant HSP60 in the cytosolBioorganic & Medicinal ChemistryDecember 1, 2022Siddhi Chitre, Anne-Marie Ray, Mckayla Stevens, Emma H. Doud, Hope Liechty, Alex Washburn, Katelyn Tepper, Jared Sivinski, Heather M. O'Hagan, Millie M. Georgiadis, et al.and 100 ng/ml hydrocortisone (Sigma #H0888); and non-cancerous FHs 74Int small intestine cells (ATCC #CCL-241) in Hybri-Care medium (ATCC #46-X) supplemented with 30 ng/ml epidermal growth factor (EGF, Gibco #PHG0313).

(12) MiR-203 is an anti-obese microRNA by targeting apical sodium-dependent bile acid transporteriScienceAugust 19, 2022Xin Liu et al.taken for further experiments. HIEC cell line The HIEC (Human intestinal epithelial cell) cell line (CCL-241) was purchased from ATCC and cultured in matched Hybri-Care Medium (46-X, ATCC) containing 30 ng/mL

(13) The Contrasting Delayed Effects of Transient Exposure of Colorectal Cancer Cells to Decitabine or AzacitidineCancersMarch 16, 2022Pawlak A, Chybicka K, Zioło E, Strządała L, Kałas W.µM 2β-mercaptoethanol, 10% FBS. The human normal small intestine epithelial cell line FHs 74 Int (#CCL-241, ATCC) was cultured in Hybri-Care medium (ATCC) supplemented with 1.5 g/L sodium bicarbonate (POCH),

(14) In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea TreatmentFrontiers in PharmacologyNovember 26, 2021Tomas Kudera, Barbora Fiserova, Marie Korytakova, Ivo Doskocil, Hana Salmonova, Edgardo E. Tulin, Samnang Nguon, Marlito M. Bande, Ladislav Kokoskaafter every use. Cell Cultures One representative of normal intestinal cell line (FHs 74 Int [ATCC CCL 241]) and two of cancer intestinal cell lines (Caco-2 [ATCC HTB 37]) and HT-29 [ATCC HTB 38]) were purchased

(15) Distigmasterol-Modified Acylglycerols as New Structured Lipids—Synthesis, Identification and CytotoxicityMoleculesNovember 12, 2021Magdalena Rudzińska, Aleksandra Grudniewska, Anna Chojnacka, Witold Gładkowski, Gabriela Maciejewska, Anna Olejnik, Katarzyna KowalskaEffect of dStigMAs on (A) normal small intestinal epithelial cells (FHs 74 Int, ATCC® CCL241™), (B) normal colonic epithelial cells (CCD 841CoN, ATCC® CRL-179™), and (C) normal liver cells (THLE-2,

(16) A Pilot Study To Establish an In Vitro Model To Study Premature Intestinal Epithelium and Gut Microbiota InteractionsmSphereOctober 27, 2021Justin Gibbons, Ji Youn Yoo, Tina Mutka, Maureen Groer, Thao T. B. HoDiego, CA, USA) to generate 250-bp paired-end reads. Cell culture. FHs-74 int cells (human, ATCC CCL 241), human fetal intestinal epithelial cells, were cultured in medium consisting of Opti-MEM (ThermoFisher,

(17) Cell-Main Spectra Profile Screening Technique in Simulation of Circulating Tumour Cells Using MALDI-TOF Mass SpectrometryCancers (Basel)July 27, 2021Wararat Chiangjong, Sebastian Chakrit Bhakdi, Noppawan Woramongkolchai, Thitinee Vanichapol, Nutkridta Pongsakul, Suradej Hongeng, Somchai ChutipongtanateAmerican Type Culture Collection (ATCC) including fetal intestinal cells (FHs74Int; accession number: CCL-241), T-cell leukaemia (Jurkat; TIB-152), neuroblastoma (SK-N-SH; accession number: HTB-11, and SH-SY5Y;

(18) Exploiting the HSP60/10 chaperonin system as a chemotherapeutic target for colorectal cancerBioorganic & Medicinal ChemistryJune 1, 2021Anne-Marie Ray, Nilshad Salim, Mckayla Stevens, Siddhi Chitre, Sanofar Abdeen, Alex Washburn, Jared Sivinski, Heather M. O'Hagan, Eli Chapman, Steven M. Johnsonand 100 ng/ml hydrocortisone (Sigma #H0888); non-cancerous FHs 74 Int small intestine cells (ATCC #CCL-241) in Hybri-Care medium (ATCC #46-X) supplemented with 30 ng/ml epidermal growth factor (EGF, Gibco #PHG0313);

(19) Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileumScientific reportsJanuary 28, 2021Liu L, Aron CZ, Grable CM, Robles A, Liu X, Liu Y, Fatheree NY, Rhoads JM, Alcorn JL.IEC-6 epithelial cell line (#CRL-1592; ATCC, Manassas, VA) human fetal FHs74int epithelial cells (#CCL-241; ATCC, Manassas, VA), the HEK293 cell line (#CRL-1573; ATCC, Manassas, VA) and the 293/hTLR4-HA cell

(20) Comprehensive studies on the properties of apple juice treated by non-thermal atmospheric plasma in a flow-through systemScientific ReportsDecember 3, 2020Anna Dzimitrowicz, Aleksandra Bielawska-Pohl, Pawel Pohl, Piotr Cyganowski, Agata Motyka-Pomagruk, Tymoteusz Klis, Malgorzata Policht, Aleksandra Klimczak, Piotr Jamrozadenocarcinoma, was obtained from ATCC. The normal human intestinal epithelial cell line FHs 74 Int (ATCC CCL-241) was immortalized according to the previously published method to receive stable cell lines23. Both

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FHs 74 Int from ATCC

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