COLO 201 from ATCC

Product Specs
ItemCOLO 201
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCCL-224
TypeCell Biology
SpeciesHomo sapiens
TissueColon
DiseaseAdenocarcinoma Colorectal Dukes type D
Cell LineCOLO 201
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Colon
Age70 years

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

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Website: http://www.atcc.org

(1) Itaconate‐Related Gene Signatures as Prognostic Markers in Colon Cancer: Insights From Transcriptomic and Spatial AnalysisHuman MutationJanuary 10, 2026Tingting Zhang, Jianchao Meng, Qingyun Wang, Peng Zhang, Hui Li, Hailang Wei, Denggang Chen, Chen Bai, Sujit NairCollection (ATCC) CCL‐227, RRID:CVCL_0547) and COLO201 (male, colon, metastatic site: ascites, ATCC CCL‐224, RRID:CVCL_0210) were obtained from the ATCC (Manassas, Virginia, United States) in 2023. Cells were

(2) Development of a bispecific CDH17-GUCY2C ADC bearing the ferroptosis inducer RSL3 for the treatment of colorectal cancerCell Death DiscovJuly 28, 2025Ying Zhang, Jing Du, Xiaohong Cui, Yuhang Ling, Chengwu TangLS1034 (ATCC®CRL-2158), LS-180 (ATCC®CL-187), SW620 (ATCC®CCL-227), LOVO (ATCC®CCL-229), COLO-201 (ATCC®CCL-224), RKO (ATCC®CRL-2577), SW1463 (ATCC®CCL-234), SW403 (ATCC®CCL-230) cell lines were obtained from the

(3) Blocker-SELEX: a structure-guided strategy for developing inhibitory aptamers disrupting undruggable transcription factor interactionsNature CommunicationsAugust 8, 2024Tongqing Li, Xueying Liu, Haifeng Qian, Sheyu Zhang, Yu Hou, Yuchao Zhang, Guoyan Luo, Xun Zhu, Yanxin Tao, Mengyang Fan, et al.using GraphPad Prism 7 (GraphPad Software, Inc., USA) with the Two-sided Student’s t-test. HCT116 (CCL-224) cell line was generously gifted form Zhejiang Cancer Hospital. MDA-MB-468 (HTB-132), HEK293T (ACS-4500),

(4) Interactions between free-living amoebae and Cryptosporidium parvum: an experimental studyParasiteAugust 21, 2023Marion Lefebvre, Romy Razakandrainibe, Damien Schapman, Arnaud François, Damien Genty, Ludovic Galas, Isabelle Villena, Loic Favennec, Damien Costa(used for evaluation of oocyst infectivity) Human ileocecal adenocarcinoma HCT-8 cell lines (ATCC CCL-224) were used for cell culture investigations. HCT-8 cells were maintained in Rosewell Park Memorial Institute

(5) Antitumor activities of a defucosylated anti‑EpCAM monoclonal antibody in colorectal carcinoma xenograft modelsInternational journal of molecular medicineFebruary 1, 2023Li G, Suzuki H, Ohishi T, Asano T, Tanaka T, Yanaka M, Nakamura T, Yoshikawa T, Kawada M, Kaneko MK, et al.Caco-2 (ATCC HTB-37), HCT116 (ATCC CCL-247), HT-29 (ATCC HTB-38), LS174T (ATCC CL-188), COLO201 (ATCC CCL-224), HCT-8 (ATCC CCL-244) and SW1116 (ATCC CCL-233), were purchased from the ATCC. HCT-15 (TKG 0504), COLO205

(6) Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myelomaNature communicationsSeptember 17, 2022Yang Y, Bolomsky A, Oellerich T, Chen P, Ceribelli M, Häupl B, Wright GW, Phelan JD, Huang DW, Lord JW, et al.line,ATCC,HTB-39 LS513,Human colon adenocarcinoma cell line,ATCC,CRL-2134 HCT8,Human colon adenocarcinoma cell line,ATCC,CCL-224 ASPC1,Human pancreatic adenocarcinoma cell line,ATCC,CRL-1682 PANC1,Human pancreatic adenocarcinoma

(7) Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2Journal of Virological MethodsJanuary 1, 2022Erin E. Schirtzinger, Yunjeong Kim, A. Sally Daviscell lines. 2 Materials and methods 2.1 Cell lines Human ileocecal adenocarcinoma (HRT-18) (ATCC CCL-224) cells were maintained in Roswell Park Memorial Institute 1640 (RPMI-1640) (ATCC formulation: Gibco,

(8) The enteric pathogen Cryptosporidium parvum exports proteins into the cytosol of the infected host celleLifeDecember 6, 2021Dumaine JE, Sateriale A, Gibson AR, Reddy AG, Gullicksrud JA, Hunter EN, Clark JT, Striepen B.parasite line expressing extra copy of MEDLE2 with I36 mutated to alanine Cell line (human),HCT-8,ATCC,CCL-224; RRID:CVCL_2478, Cell line (human),HEK293T,ATCC,CRL-3216; RRID:CVCL_0063, Cell line (Escherichia coli),GC5,Genesee

(9) A Novel Antagonistic CD73 Antibody for Inhibition of the Immunosuppressive Adenosine PathwayMolecular cancer therapeuticsNovember 1, 2021Wurm M, Schaaf O, Reutner K, Ganesan R, Mostböck S, Pelster C, Böttcher J, de Andrade Pereira B, Taubert C, Alt I, et al.receptor, or with chemotherapy. Materials and Methods Cell lines and cell culture COLO201 cells (ATCC, CCL-224), NCI-H460 cells (ATCC, HTB-177), and 4T1 cells (ATCC, CRL-2539) were cultivated in RPMI1640 (ATCC,

(10) Detection of Infectious Cryptosporidium parvum Oocysts from Lamb’s Lettuce: CC–qPCR’s IntakeMicroorganismsJanuary 21, 2021Sophie Kubina, Damien Costa, Loïc Favennec, Gilles Gargala, Angélique Rousseau, Isabelle Villena, Stéphanie La Carbona, Romy Razakandrainibeuse. 2.2. Cell Line and Routine Cell Culture The human ileocecal adenocarcinoma HCT-8 cell line (ATCC CCL-224) was maintained in Rosewell Park Memorial Institute “RPMI“1640 medium (Lonza, Verviers, Belgium) supplemented

(11) Nuclear protein 1 imparts oncogenic potential and chemotherapeutic resistance in cancerCancer LettersDecember 1, 2020Anthony Murphy, Max Costa(ATCC® HTB-37) "(X. Li et al., 2012)",HRT-18 (HCT-8),+,Ileocecal colorectal adenocarcinoma (ATCC® CCL-224) HCT116,+,Colorectal carcinoma (ATCC® CCL-247),"(S. S. Chen et al., 2015)" Primary colorectal tumors,+,Uncharacterized,"(Wang

(12) Synthesis, Characterization, and Biologic Activity of New Acyl Hydrazides and 1,3,4-Oxadiazole DerivativesMoleculesJuly 21, 2020Irina Zarafu et al.activity was assessed after incubation for 24 h at 37 °C. 3.5. Cell Culture Two cell lines, HCT-8 (CCL-224) and HT-29 (ATCC-HTB-38), were used to evaluate the activity of new compounds on eukaryotic cells. These

(13) Antitumor activity of the polo-like kinase inhibitor, TAK-960, against preclinical models of colorectal cancerBMC CancerDecember 1, 2018Peter J. Klauck et al.colorectal cancer cell lines used in this study were: CL-11(DSMZ ACC 467), CL-34(DSMZ ACC520), COLO201 (ATCC® CCL-224™), COLO205 (ATCC® Klauck et al. BMC Cancer (2018) 18:136 CCL-222™), COLO678 (DSMZ ACC 194), DLD1 (ATCC®

(14) Fabrication and Cytotoxicity of Fucoidan-Cisplatin Nanoparticles for Macrophage and Tumor CellsMaterials (Basel)March 14, 2017Pai-An Hwang, Xiao-Zhen Lin, Ko-Liang Kuo, Fu-Yin Hsu(100 µg/mL), and cells were maintained at 37 ◦ C in an atmosphere of 5% CO2 . HCT-8 cells (ATCC No. CCL-224), the human ileocecal adenocarcinoma tumor cells, were obtained from the American Type Culture Collection.

(15) Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacitiesOncoimmunologyJanuary 1, 2017Van Caeneghem Y, De Munter S, Tieppo P, Goetgeluk G, Weening K, Verstichel G, Bonte S, Taghon T, Leclercq G, Kerre T, et al.H508 (ATCC CCL 253) are CEA-expressing carcinoma cell lines, Colo320 (ATCC CCL 220), Colo201 (ATCC CCL 224) and H716 (ATCC CCL251) are CEA− colon carcinoma cell lines, FM3, MEL624 and MEL526 are HLA-A2+ melanoma

(16) Bovine gamma/delta T cells are stimulated by bovine coronavirus antigens identified by a soluble T cells receptorMedycyna WeterynaryjnaJanuary 1, 2017Felix N. Tokaligands. The bovine coronavirus (ATCC ® VR-874™, BCov) was propagated in HRT-18 cells (COLO 201 (ATCC® CCL-224™). Supernatants were clarified and concentrated, and later used in viral ligand detection. To assess

(17) Downregulation of Enhancer of Zeste Homolog 2 (EZH2) is essential for the Induction of Autophagy and Apoptosis in Colorectal Cancer CellsGenes (Basel)October 3, 2016Yizhou Yao, Hao Hu, Yong Yang, Guoqiang Zhou, Zengfu Shang, Xiaodong Yang, Kang Sun, Shenghua Zhan, Zhengyuan Yu, Peiyao Li, et al.Materials and Methods 2.1. Cell Lines and DZNep The human CRC cell lines (RKO, HCT116, SW480 and CCL224) were purchased from the Chinese Academy of Sciences (Shanghai, China) and cultured in the six-well

(18) The Quorum Sensing Peptides PhrG, CSP and EDF Promote Angiogenesis and Invasion of Breast Cancer Cells In VitroPLOS ONEMarch 17, 2015Bart De Spiegeleer, Frederick Verbeke, Matthias D’Hondt, An Hendrix, Christophe Van De Wiele, Christian Burvenich, Kathelijne Peremans, Olivier De Wever, Marc Bracke, Evelien Wynendaelerelationship. Materials and Methods Cell culture The epithelial breast adenocarcinoma cell line MCF-7/AZ (ATCC CCL-224) was grown in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 10% (V/V) foetal

(19) Crosstalk between the microbiome and cancer cells by quorum sensing peptidesPeptidesFebruary 1, 2015Evelien Wynendaele, Frederick Verbeke, Matthias D’Hondt, An Hendrix, Christophe Van De Wiele, Christian Burvenich, Kathelijne Peremans, Olivier De Wever, Marc Bracke, Bart De SpiegeleerMETHODS 2.1. Cell culture An epithelial subclone from human ileocecal colorectal adenocarcinoma cells (ATCC® CCL224), i.e. HCT-8/E11, was grown in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented

(20) Relationship between Expression of Onco-Related miRNAs and the Endoscopic Appearance of Colorectal TumorsInternational Journal of Molecular SciencesJanuary 9, 2015Yoshihito Nakagawa et al.Culture Collection (ATCC)®, CCL-228™; Manassas, VA, USA), DLD-1 (ATCC®, CCL-221™), and COLO201 (ATCC®, CCL-224™) were grown in RPMI-1640 medium supplemented with 10% (v/v) heat-inactivated FBS (Sigma, St. Louis,

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COLO 201 from ATCC

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