MeT-5A from ATCC

Product Specs
ItemMeT-5A
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-9444
TypeCell Biology
SpeciesHomo sapiens
TissueMesothelium
Cell LineMeT-5A
Validated Species ReactivityHuman (Hu)
Cell Typeepithelial cell
ApplicationsThe cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) PDK4-driven metabolic reprogramming enhances mesothelial cell invasion in colorectal cancer peritoneal metastasisActa Biochimica et Biophysica SinicaJanuary 28, 2026Lingyu Han, Qinyuan Zhang, Yuchen Wu, Wenqin Luo, Shaobo Mo, Hongsheng Fang, Qingguo Li, Renjie Wang, Guoxiang Cai, Weixing DaiTokyo, Japan). Cell culture and reagents Human immortalized mesothelial cell line (MET-5A; ATCC® CRL-9444™) was purchased from Yuanfeng (Shanghai, China) and cultured in Medium 199 (M199, 31150022; Gibco, Thermo

(2) The Iodine-Dextrin-Lithium Complex: Morphology, Antibacterial Activity, and CytotoxicityMoleculesDecember 18, 2025Daniil Shepilov, Seitzhan Turganbay, Ardak Jumagaziyeva, Tamara Bukeyeva, Dana Askarova, Didar Bolatova, Yerlan Doszhanov, Arman Zhumazhanov, Almagul Kerimkulova, Karina Saurykova, et al.cervical adenocarcinoma, ATCC-CCL-2, USA), MeT-5A (SV40—immortalized human mesothelial cell line, ATCC-CRL-9444, USA) were used for studying the antitumor effect. Cell cultures—MDCK kidney cells of the Madin–Darby

(3) Exploration of the anticancer properties of Caffeic Acid in malignant mesothelioma cellsMed OncolJune 10, 2025Dayk Muratoglu, Gulseren Turhal, Busra Demirkan, Izel Nermin Baslilar, Nimet Sule Yuncu, Asuman Demiroglu-Zergeroglufuture treatment option. Materials and methods Cell culture and preparation of substances MeT-5A (CRL-9444) cell line was obtained from ATCC; SPC111 (11120716) and SPC212 (11120717) cells were purchased from

(4) Self-Assembled Cannabigerol-Based Nanoparticles: Design, Synthesis, and Antiproliferative ActivityPharmaceuticsMay 11, 2025Arianna Amenta, Giulia Nordio, Francesco Piazzola, Maria Luisa Di Paolo, Fabio Milani, Martina Giacomini, Andrea Citarella, Umberto Ciriello, Giuseppe Paladino, Sara Pellegrino, et al.Specifically, we used LN-229 (ATCC CRL-2611), HT-29 (ATCC HTB-38), and MSTO-211H (ATCC CRL-2081). MeT-5A (ATCC CRL-9444), MSTO-211H (human biphasic mesothelioma), and Met-5A (human mesothelium) cells were grown in RPMI 1640

(5) Imaging gastric cancer metastasis progression in an organotypic, three-dimensional functional model of the human peritoneumPleura and PeritoneumMarch 6, 2025Arianna Castagna, Frank-Jürgen Weinreich, Andreas Brandl, Janine Spreuer, Nicola Herold, Birgit Schittek, Marc André Reymond, Wiebke Solassavailable on the market (MeT-5A). MeT-5A were purchased from American Type Culture Collection (ATCC® CRL-9444™, LGC Standards, Germany) and cultured in Medium RPMI 1640+GlutaMax™-I (Invitrogen) culture medium supplemented

(6) Methylglyoxal-Stimulated Mesothelial Cells Prompted Fibroblast-to-Proto-Myofibroblast TransitionInt J Mol SciJanuary 19, 2025Yu-Syuan Wei, Su-Yi Tsai, Shuei-Liong Lin, Yi-Ting Chen, Pei-Shiue TsaiMaterials and Methods 4.1. Cell Culture and In Vitro Injury Model Human mesothelial cell line (MeT-5A, # CRL-9444™), and human fibroblast cell line (MRC-5, #CCL-171™) were obtained from ATCC (Manassas, VA, USA). MeT-5A

(7) Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacyCell Reports MedicineOctober 15, 2024Kua Liu, Lingkai Kong, Huawei Cui, Louqian Zhang, Qilei Xin, Yan Zhuang, Ciliang Guo, Yongzhong Yao, Jinqiu Tao, Xiaosong Gu, et al.MDA-MB-231 cells,ATCC,Cat#CRM-HTB-26 HCT116 cells,ATCC,Cat#CCL-247EMT HepG2 cells,ATCC,Cat#HB-8065 MET-5A cells,ATCC,Cat#CRL-9444 THP-1 cells,ATCC,Cat#TIB-202 HEK 293T cells,ATCC,Cat#CRL-1573 RAW264.7 cells,ATCC,Cat#TIB-71 ,, Experimental models

(8) EWS-WT1 fusion isoforms establish oncogenic programs and therapeutic vulnerabilities in desmoplastic small round cell tumorsNature CommunicationsAugust 28, 2024Gaylor Boulay, Liliane C. Broye, Rui Dong, Sowmya Iyer, Rajendran Sanalkumar, Yu-Hang Xing, Rémi Buisson, Shruthi Rengarajan, Beverly Naigles, Benoît Duc, et al.from the ATCC and media from Life Technologies. JN-DSRCT127 (male gender), MeT-5A (male gender, ATCC CRL-9444) and LentiX HEK293T (female gender, Takara #632180) cells were respectively grown in Dulbecco’s Modified

(9) Decellularisation and Characterisation of Porcine Pleura as Bioscaffolds in Tissue EngineeringJournal of Tissue Engineering and Regenerative MedicineJuly 8, 2024Thirapurasundari Vikranth, Tina Dale, Nicholas R. Forsythsterilisation efficiency. 2.10. In Vitro Cytotoxicity Assay Human mesothelial cells (MeT-5A, ATCC CRL-9444™, Virginia, USA) were cultured in cFAD medium [34], composed of DMEM and Hams F-12 (Lonza), (3 : 1,

(10) Enhancing PKA-dependent mesothelial barrier integrity reduces ovarian cancer transmesothelial migration via inhibition of contractilityiScienceJune 21, 2024Jazwinska DE, Cho Y, Zervantonakis IK.Medical School",N/A ZTGFP,"Laboratory of Dr. Joan Brugge, Harvard Medical School",N/A MeT-5A,ATCC,CRL-9444 Human Mesothelial Cells,ZenBio,N/A HT1080,"Laboratory of Dr. Roger Kamm, MIT",N/A CACO-2,"Laboratory

(11) Protocol for the isolation of GFP-expressing ferroptosis-dependent extracellular vesicles in in vitro cell culture modelsSTAR ProtocolsMarch 15, 2024Fumiya Ito, Izumi Yanatori, Katsuhiro Kato, Shinya Toyokuni,Experimental models: Cell lines ,, HEK293T,Riken Bioresource center,RCB2202 HT1080,JCRB,JCRB9113 MeT-5A,ATCC,CRL-9444 THP-1,Riken Bioresource center,RCB1189 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, FUGW,Addgene,http://n2t.net/addgene:14883

(12) Targeting metabolic fluxes reverts metastatic transitions in ovarian canceriScienceNovember 17, 2023Garhima Arora, Mallar Banerjee, Jimpi Langthasa, Ramray Bhat, Samrat ChatterjeeScience",HTB-161 SK-OV-3,"Kind gift from Prof. Rajan Dighe, Indian Institute of Science",HTB-77 MeT-5A,ATCC,CRL-9444 COV362,ECACC,07071910 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Recon3D

(13) Genomic and Transcriptomic Analyses of Malignant Pleural Mesothelioma (MPM) Samples Reveal Crucial Insights for Preclinical TestingCancersMay 18, 2023Laure A, Rigutto A, Kirschner MB, Opitz L, Grob L, Opitz I, Felley-Bosco E, Hiltbrunner S, Curioni-Fontecedro A.(RCB RCB2292), ONE58 (ECACC 10092313), ZL55 (ECACC 12050301), and the MeT-5A control cell line (ATCC CRL-9444). Cells were cultured in a humidified incubator at 37 °C and 5% CO2 in DMEM (ZL55, Mero82 and MeT-5A)

(14) Glucocorticoids increase tissue cell protection against pore-forming toxins from pathogenic bacteriaCommunications BiologyFebruary 17, 2023Thomas J. R. Ormsby, Sian E. Owens, Matthew L. Turner, James G. Cronin, John J. Bromfield, I. Martin Sheldonisolated from a normal individual and immortalized with SV40. Normal human mesothelial MET5A cells (CRL-9444; ATCC) were isolated from pleural fluids obtained from non-cancerous individuals and immortalized with

(15) Asbestos Fibers Enhance the TMEM16A Channel Activity in Xenopus OocytesMembranes (Basel)February 1, 2023Annalisa Bernareggi, Martina Zangari, Andrew Constanti, Paola Zacchi, Violetta Borelli, Alessandro Mangogna, Paola Lorenzon, Giuliano ZabucchiFraction from the MeT5a Cell Line A total of 100 × 106 Met5a cells (a human mesothelial cell line, ATCC CRL-9444) were grown in M199 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), L-glutamine

(16) Activated Leukocyte Cell Adhesion Molecule (ALCAM), a Potential 'Seed' and 'Soil' Receptor in the Peritoneal Metastasis of Gastrointestinal CancersInternational journal of molecular sciencesJanuary 3, 2023Yang YM, Ye L, Ruge F, Fang Z, Ji K, Sanders AJ, Jia S, Hao C, Dou QP, Ji J, et al.Collection (ATCC/LGC Standard, Teddington, England, UK). Human immortalized mesothelial cells, MET5A (CRL-9444) was also obtained from ATCC. Cells were cultured in a humidified incubator at 37 °C, 5% CO2 and 95%

(17) Therapeutic mechanism of baicalein in peritoneal dialysis-associated peritoneal fibrosis based on network pharmacology and experimental validationFrontiers in pharmacologyJanuary 1, 2023Lu X, Wu K, Jiang S, Li Y, Wang Y, Li H, Li G, Liu Q, Zhou Y, Chen W, et al.saline. Cell culture MeT-5A cells were purchased from the American Type Culture Collection (ATCC CRL-9444, United States) and were cultured at 37 C with 5% CO2 in Medium 199 containing 1.5 g/L sodium bicarbonate,

(18) Sucrose-based cryoprotective storage of extracellular vesiclesExtracellular VesicleDecember 1, 2022Sierra A. Walker, Irina Davidovich, Yubo Yang, Andrew Lai, Jenifer Pendiuk Goncalves, Vatsal Deliwala, Sara Busatto, Shane Shapiro, Na’ama Koifman, Carlos Salomon, et al.Cell culture Human MDA-MB-231 breast cancer cells (HTB-26; ATCC) and human MeT-5A pleural mesothelial cells (CRL-9444; ATCC) were cultured and maintained in high-glucose Dulbecco’s modified Eagle medium (DMEM, Life Technologies)

(19) SMG6 regulates DNA damage and cell survival in Hippo pathway kinase LATS2-inactivated malignant mesotheliomaCell death discoveryNovember 5, 2022Suzuki K, Tange M, Yamagishi R, Hanada H, Mukai S, Sato T, Tanaka T, Akashi T, Kadomatsu K, Maeda T, et al.from the patients. Cell lines and cell culture The human immortalized mesothelial cell line MeT-5A (CRL-9444) and human malignant mesothelioma cell lines (NCI-H2052 and NCI-H2373) were purchased from ATCC. HOMC-D4

(20) Involvement of Mitochondrial Dysfunction in the Inflammatory Response in Human Mesothelial Cells from Peritoneal Dialysis EffluentAntioxidants (Basel, Switzerland)November 4, 2022Ramil-Gómez O, López-Pardo M, Fernández-Rodríguez JA, Rodríguez-Carmona A, Pérez-López T, Vaamonde-García C, Pérez-Fontán M, López-Armada MJ.performing the experiments and during cell stimulation. Mesothelial cell line Met5A (ATCC, reference CRL-9444) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Lonza) supplemented with 10% heat-inactivated

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