Fig 2: Graphical representation of the result table. The flow cytometric Antibody Bound per Cell (ABC) values and Resonance Units (RU) values of SPRi of five different cell lines are shown for 28 markers. In total, 16 markers were left out because these markers did not show any or a very weak response for all five cell lines (for the full unedited table including correlations see Supplemental Table S1). The total SPR signal per column was used to normalize the ABC signal with the SPR signal. It shows that all cell lines are different but some lines are “closer” to each other. E.g., the cell line KG1a and MG63 (ATCC CRL-1427™) behave similar over the various markers but are different with respect to CD45.

Fig 3: Morphological characteristics and changes in MG-63 osteosarcoma cells and non-neoplastic MC3T3-E1 subclone 14 osteoblast controls effected by ALAPP. Osteosarcoma and osteoblasts were treated with ALAPP at various concentrations or with PBS as a control for 48 h. The morphological characteristics and changes were evaluated using an inverted phase contrast microscope EVOS FL Auto Cell Imaging System 20× LPlan FH objective (catalog No. AMEP-4682; Thermo Fisher Scientific). Untreated cells (A and D), and the effect of only 2 concentrations, 20 μg/mL ALAPP (B and E) and 50 μg/mL ALAPP (C and F), are shown to highlight the substantial differences in cell morphology. A, B, and C. Mouse osteoblasts MC3T3-E1 subclone 14 derived from osteoblast precursors of the parietal calvaria (ATCC CRL-2594); D, E, and F. Human osteosarcoma cells MG-63 (ATCC CRL-1427). Scale bars 100 μm. ALAPP, antlion antiproliferative polypeptide; PBS, phosphate-buffered saline.