SK-BR-3 [SKBR3] from ATCC

Product Specs
ItemSK-BR-3 [SKBR3]
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberHTB-30
ApplicationsThis cell line is suitable as a transfection host
TypeCell Biology
SpeciesHomo sapiens
TissueMammary gland, Breast
Cell LineSK-BR-3
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Breast
Age43 years
DiseaseAdenocarcinoma

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Debio 1562M CD37-targeting ADC is highly active and well tolerated in preclinical models of AML and MDSCell Reports MedicineMay 19, 2026Léo Marx, Josée Hue-Perron, Alain Monjardet, Selena Vigano, Christophe Chardonnens, Kaitlin Morse, Esthela Artiga, Marie-Claude Roubaudi-Fraschini, René Wuttke, Riccardo Colombo, et al.lines,Experimental models: Cell lines,Experimental models: Cell lines ,, MOLM-13,DSMZ,ACC 554 SK-BR-3,ATCC,HTB-30 THP-1,ATCC,TIB-202 WSU-DLCL-2,DMSZ,ACC 575 THP-1 WT and CD37 KO,Laboratory of Karilyn Larkin,Jeremy

(2) Cancer-associated fibroblast-derived protein S100-A11 influences the response to anti-HER2 therapies in HER2-positive breast cancerNeoplasiaMay 19, 2026Melani Luque, Miriam Morales-Gallego, Marta Sanz-Álvarez, Claudia Arguiñano, Yamileth Rangel, Natalia Ramírez-Merino, Mengjuan Qin, Ana Rovira, Joan Albanell, Juan Madoz-Gúrpide, et al.(ATCC Cat# HTB-20; RRID:CVCL_0179), AU-565 (ATCC Cat# CRL-2351; RRID:CVCL_1074), SK-BR-3 (ATCC Cat# HTB-30; RRID:CVCL_0033), MCF-7 (ATCC Cat#: HTB-22; RRID: CVCL_0031) and the human T lymphocyte cell line Jurkat

(3) Selective biological activity of parthenolide derivatives - stizolin, stizolicin, and izospiciformin - isolated from the leaves of Stizolophus balsamita, in different breast cancer molecular subtypes in vitroFront PharmacolMay 11, 2026Joanna Nawrot, Ewa Totoń, Iga Dziechciowska, Agnieszka Boruta, Małgorzata Gołębiowska, Małgorzata Idzik, Mariusz Kaczmarek, Błażej Rubiś, Justyna Gornowicz-Porowska, Natalia Lisiakline (ER+, PR+, HER2-) were obtained from the American Type Culture Collection (ATCC, HTB-22, HTB-26, HTB-30, CRL-10782, respectively). The MCF7 and MDA-MB-231 cells were maintained in RPMI 1640 medium supplemented

(4) A novel insulin-like growth factor II-based masking domain for conditional activation of therapeutic antibodiesMAbsMay 7, 2026Lihua Shi, Minseon Cho, Motohiko Nishida, Mark L. Chiu, Di ZhangQuanti-Blue detection kit (InvivoGen, rep-qbs). SK-BR-3 cell viability assay SK-BR-3 cells (ATCC, HTB-30) were seeded at 3,000 cells per well in 96-well plates and treated with serial dilutions of test antibodies.

(5) BIRC5 Promoter-Driven Nanodrugs Suppress BIRC5-Positive Cancers Independent of ABCB1 Status and IDO1 ExpressionInt J NanomedicineApril 28, 2026Yung-Chieh Chang, Wei-Lun Huang, Wu-Chou Su, Euphemia Leung, Fong-Yu Cheng, Chun Hei Antonio Cheung(cervical carcinoma) (ATCC, cat. no. CRL-3596 TM), and SK-BR-3 (breast adenocarcinoma) (ATCC, cat. no. HTB-30 TM) were obtained from the American Type Culture Collection (ATCC) (Table 1). MIA PaCa-2 cells were

(6) Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂-COX-2-NF-κB pathwayMol Biol RepApril 24, 2026Egemen Çakırlı, İpek Bedir, Yağmur Biliz, Özgür Yılmaz, Ş. Güniz Küçükgüzel, Dilek TelciPerkinElmer Spectrum BX spectrometer. Cell viability and cell cycle assay AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described

(7) Theranostic vNAR-Based Immunoconjugates Achieve Selective Intracellular Cisplatin Delivery in Embedded 3D HER2-Positive Breast Cancer In Vitro ModelPharmaceuticals (Basel)April 17, 2026Andrea C. Alfonseca-Ladrón de Guevara, Alejandro Manzanares-Guzmán, Jessica A. Badillo-Mata, Mirna Burciaga-Flores, Pavel H. Lugo-Fabres, Tanya A. Camacho-Villegasprotocol. 4.4. Immunofluorescence Analyses U87-MG (ATCC HTB-14), HBEC-5i (ATCC CRL-3245), and SKBR3 (ATCC HTB-30) cells were cultured in their respective media according to ATCC guidelines and maintained at 37 °C

(8) Promotion of breast cancer by the DNPH1 enzymeiScienceApril 17, 2026Sangphil Oh, Yuan Sui, Sook Shin, Ralf Janknechtlines,Experimental models: cell lines ,, Mouse: 4T1,ATCC,CRL-2539 Human: MCF10A,ATCC,CRL-10317 Human: SKBR3,ATCC,HTB-30 Human: MDA-MB-453,ATCC,HTB-131 Human: MCF7,ATCC,HTB-22 Human: T47D,ATCC,HTB-133 Human: ZR-75-1,ATCC,CRL-1500 Human:

(9) LINC01929 mediates breast cancer immunosuppression and is an immunotherapy targetiScienceApril 17, 2026Jaganathan Venkatesh, Marie-Claire D. Wasson, Raj Pranap Arun, Hannah F. Cahill, Olivia L. Walker, Cheryl A. Dean, Wasundara Fernando, Maya R. MacLean, Meghan E. McLean, Bakhmala Khan, et al.RRID:CVCL_0031 MDA-MB-231,ATCC,CRM-HTB-26 RRID:CVCL_0062 MDA-MB-468,ATCC,HTB-132 RRID:CVCL_0419 SKBR3,ATCC,HTB-30; RRID:CVCL_0033 T-47D,ATCC,HTB-133; RRID:CVCL_0553 Hs578Bst,ATCC,HTB-125RRID:CVCL_0807 MCF10A,ATCC,RRID:CVCL_0598CRL-10317 ,, Experimental models

(10) Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling AxisInternational Journal of Molecular SciencesMarch 31, 2026Grace L. Wong, Munazza S. Khan, Sara Manore, Shivani Bindal, Ravi Singh, Hui-Wen Loand Expression Plasmids SKBR3 cells were purchased from the American Type Culture Collection (cat. # HTB-30; ATCC). CN34 and CN34-BRM cells, the brain metastatic variant derived from TNBC CN34 cells, were a kind

(11) Elucidating the relationship between affinity and potency in the performance of therapeutic IgEScientific ReportsMarch 30, 2026Francesca Marano, Callum McKenzie, James R. Birtley, Sadaf A. Hussain, Olivia Macleod, Alexander Goodacre, Shuang Wu, Oliver E. Amin, Nikhil Faulkner, John Devlin, et al.liquid nitrogen until data collection. Cell lines for functional immunological assays SKBR3 (ATCC, HTB-30) and SKBR3-NIR cells were cultured in McCoy’s 5a Medium (Gibco; 16,600–082) supplemented with 10% Fetal

(12) GCNT3 and ST3GAL1 expression correlates with HER2 status and MUC1/β-catenin/Cyclin D1 axis in breast cancerBMC CancerMarch 19, 2026Rohitesh Gupta, Indresh K Gopalsamy, Aisha Shigna Nadukkandy, Ankit Singh, Charan Raju Tangella, Liza Esther Alexander, Rekha A Nair, Marco Cordani, Lekha Dinesh KumarCatalog CRL-1573) in Dulbecco’s Modified Eagle Medium (DMEM). HER2 positive cell line SKBR3 (ATCC Catalog HTB-30) was cultured in McCoy’s 5 A medium. All cell lines were grown in the presence of 10% FBS and 5% CO2

(13) A Novel Green Synthesis Method of Copper Nanoparticles and Their Biological Effects on Cancer and Normal CellsInt J Mol SciMarch 11, 2026Maria-Alexandra Pricop, Adina Negrea, Ioan Bogdan Pascu, Mihaela Ciopec, Petru Negrea, Iustina-Mirabela Cristea, Călin Adrian Tatu, Alexandra Ivanobtained from each subject. SKBR3 cells were purchased from the American Type Culture Collection (ATCC, HTB-30, Manassas, VA, USA). SKBR3, a well-characterized cell line, was selected to evaluate CuNPs as a representative

(14) The critical role of Atpif1 in Her2-targeted CAR-T cell therapy for solid tumor via modulation of metabolism and mtDNA-STING signal pathwayFront ImmunolMarch 2, 2026Genshen Zhong, Biao Liu, Xue Gong, Qi Wang, Shuyin Zheng, Xinyu Luo, Zhiguo Chen, Xingya Zhang, Biliang Hu, Minna Wusolid tumors. 2 Materials and methods 2.1 Cell lines The human breast cancer cell line SKBR-3 (HTB-30, ATCC) was cultured in RPMI-1640 medium (Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS)

(15) Key Role of Pro230 in the Hinge Region on the Architecture and Function of IgG1Journal of Medicinal ChemistryFebruary 26, 2026Yuuki Koseki, Yuki Yamaguchi, Michihiko Aoyama, Kentaro Hiraka, Minoru Tada, Atsuji Kodama, Akinobu Senoo, Akiko Ishii-Watabe, Takayuki Uchihashi, Kazuyoshi Murata, et al.variants. Materials and Methods Cells SK-BR-3 cells (an HER2+ human breast cancer cell line; ATCC, HTB-30) were obtained from ATCC. Jurkat/FcγR/NFAT-Luc cells, which express human FcγRI, FcγRIIa, or FcγRIIIa

(16) AXL-Driven Stemness and Hedgehog Signaling in HER2-Positive Breast Cancer with Acquired Trastuzumab Resistance: Synergistic Potential of AXL and HER2 Co-TargetingLife (Basel)February 25, 2026Asiye Busra Boz, Idris Er, Enric Arasanz Picher, Sneha SmarakanTrastuzumab-Resistant Cells The HER2-overexpressing breast cancer cell lines HCC1954 (ATCC CRL-2338) and SKBR3 (ATCC HTB-30) were selected for this study due to their documented ability to acquire resistance to trastuzumab.

(17) Interplay of Interleukin-1β and Curcumin on VEGF Expression in Breast Cancer CellsOncology ResearchFebruary 24, 2026Norbert Nass, Atanas Ignatov, Thomas KalinskiMaterials and Methods 2.1 Cells and Cell Culture All cell lines MCF-7 (ATCC-HTB-22), SK-BR-3 (ATCC-HTB-30), MDA-MB-231 (ATCC-HTB-26), and UACC-3199 (ATCC-CRL-2983) were obtained from the American Type Culture

(18) Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populationsCell ReportsFebruary 24, 2026Xiaowen Hu, Jie Huang, Jiao Yuan, Yanrong Sun, Yuxin Wang, Zhongyi Hu, Junjie Jiang, Zhiling Wang, Bingwei Wang, Meixiao Long, et al.10032308 SCC-9,ATCC,Cat# CRL-1629 SCC-15,ATCC,Cat# CRL-1623 SCC-25,ATCC,Cat# CRL-1628 SK-BR-3,ATCC,Cat# HTB-30 SK-CO-1,ATCC,Cat# HTB-39 SW 620,ATCC,Cat# CCL-227 U-87 MG,ATCC,Cat# HTB-14 UPCI-SCC-040,DSMZ,Cat#

(19) R-loop editing by DNA cytosine deaminase APOBEC3B modulates the activity of oestrogen receptor enhancersNature CommunicationsFebruary 18, 2026Chi Zhang, Yu-jing Lu, Bingjie Chen, Zhiyan Bai, Qiaoxi Zeng, Alexia Hervieu, Marco P. Licciardello, Konstantinos Mitsopoulos, Bissan Al-Lazikani, Marcello Tortorici, et al.evolution. Methods Cell culturing and cell line preparation T47D (ATCC HTB-133) and SKBR3 (ATCC HTB-30) cells were obtained from ATCC. Lenti-X 293 T cells were obtained from Clonetech. Cells were maintained

(20) Fe/57Fe-Metallacarboranes with Radiosensitizing Potential in Breast Cancer Cell Models: Comparative Study Between High- (60Co) and Low-Energy (57Co) Gamma Radiation SourcesPharmaceuticsFebruary 9, 2026Salvatore Di Maria, Diogo M. Engrácia, Catarina I. G. Pinto, João C. Waerenborgh, Bruno J. C. Vieira, Pedro Santos, Teresa Pinheiro, Miquel Nuez-Martínez, António P. Matos, Filipa Mendes, et al.Materials and Methods 2.1. Gamma Ray Irradiation Experiments For the irradiation studies, SK-BR-3 (ATCC, HTB-30) and MDA-MB-231 (ATCC, HTB-26) cells (1–2 × 104 cells/well for 2D cultures, 1250 cells/well for 3D cultures)

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SK-BR-3 [SKBR3] from ATCC

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