NCI-H441 [H441] from ATCC

Product Specs
ItemNCI-H441 [H441]
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberHTB-174
TypeCell Biology
SpeciesHomo sapiens
TissueLung
Cell LineNCI-H441
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Lung
DiseaseAdenocarcinoma Papillary
ApplicationsThe line has been used as a transfection host for expression of pulmonary surfactant protein (SP-B)

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Rab11B is required for binding and entry of recent H3N2, but not H1N1, influenza A isolatesJ VirolJune 23, 2026Allyson H. Turner, Sara A. Jaffrani, Hannah C. Kubinski, Deborah P. Ajayi, Matthew B. Owens, Conor D. Fanuele, Madeline P. McTigue, Cailey L. Appenzeller, Addington Bowling, Hannah W. Despres, et al.Salogiannis), human papillary adenocarcinoma lung cells, considered “club-like cell” (NCI-H441, ATCC HTB-174), and Madeline Darby kidney cells modified to express α2,6 sialic acid and TMPRSS2 (71) (MDCK-SIATT)

(2) Force Spectroscopy by Atomic Force Microscopy as Indicator for Cellular Microplastic UptakeInternational Journal of Molecular SciencesApril 23, 2026Tatjana Kolesnik, Kristin Öhlinger, Markus Absenger-Novak, Eleonore FröhlichLuckenwalde, Germany) cells in passages 62–81, H23 (ATCC-CRL-5800; LGC) in passages 103–121, H441 (ATCC-HTB-174; LGC) cells in passages 7–18, HCC827 (ATCC-CRL-2868; LGC) cells in passages 13–28, and Calu-3 (ATCC-HTB-55;

(3) Rab11B is required for binding and entry of recent H3N2, but not H1N1, influenza A isolatesbioRxivApril 12, 2026Allyson H. Turner, Sara A. Jaffrani, Hannah C. Kubinski, Deborah P. Ajayi, Matthew B. Owens, Conor D. Fanuele, Madeline P. McTigue, Cailey L. Appenzeller, Addington Bowling, Hannah W. Despres, et al.Salogiannis), human papillary adenocarcinoma lung cells, considered ‘club-like cell’ (NCI-H441, ATCC HTB-174) and Madeline Darby Kidney cells modified to express α2,6 sialic acid and TMPRSS271 (MDCK-SIATT) (kindly

(4) De novo design of Ras isoform selective bindersCell Chem BiolMarch 19, 2026Jason Z. Zhang, Xinting Li, Alexa Rane Batingana, Caixuan Liu, Hanlun Jiang, Kevin Shannon, Benjamin J. Huang, Kejia Wu, David BakerMEF,NCI,Cat#RPZ26379 MIA PaCa-2,ATCC,Cat#CRL-1420 H358,ATCC,Cat#CRL-5807 MOLM-13 Cells,DSMZ,Cat#ACC-554 H441,ATCC,Cat#HTB-174 Rh36,Gift from DJ. Maly lab,N/A RD,ATCC,Cat#CCL-136 H1299,Gift from R. Bayliss lab.,N/A SW1573,ATCC,Cat#CRL-2170 Experimental models

(5) Berry e-liquid flavour toxicities are not equal on an alveolar-capillary barrier cell model according to two exposure methodsFront ToxicolMarch 11, 2026Emma Filaudeau, Amelia-Naomi Sabo, Anita Lebert, Laurent Monassier, Véronique KemmelCell culture Two different cell lines were used in our study to mimic the ACB. The NCI-H441 cell line (ATCC® - HTB-174™), which is derived from a human papillary adenocarcinoma of the lung, was used as the epithelial cell

(6) Expanding the payload scope in antibody-drug conjugates by delivery of hydroxy-containing drugs through self-immolative phosphoramidatesNature CommunicationsJanuary 20, 2026Philipp Ochtrop, Anil P. Jagtap, Jan G. Felber, Simon Vogt, Sarah Herterich, Isabelle Mai, Philipp Cyprys, Saskia Schmitt, Sarah Payer, Annabel Kitowski, et al.736, DSMZ; RRID:CVCL_0033; sex: female), HCC-1569 (CRL-2330, ATCC; RRID:CVCL_1255; sex: female), H441 (HTB-174, ATCC; RRID:CVCL_1561; sex: male), MDA-MB-468 (ACC 738, DSMZ; RRID:CVCL_0419; sex: female), HCC78 (ACC

(7) Engineering Inhalable Carboxymethyl Chitosan-Swellable Microgels for Pulmonary Delivery of Charged Hydrophilic Molecules †GelsDecember 17, 2025David Encinas-Basurto, Kiley McCombs, Ernest L. Vallorz, Maria F. Acosta, Rick G. Schnellmann, Heidi M. Mansourevaluated using the resazurin reduction assay. H441, human papillary lung adenocarcinoma cells (ATCC® HTB-174™) and H358, human bronchioalveolar epithelial cells (ATCC® CRL-5807™) were purchased from the American

(8) EMB ‐01, a Tetravalent Bispecific Antibody, Inducing Co‐Degradation of EGFR and c‐Met for Enhanced Anti‐Tumor EfficacyCancer ScienceDecember 12, 2025Jing Gao, Xi Jiao, Fang Ren, Xuan Wu, Jingyun Tan, Yuezong Bai, Yan Dai, Lixia Shen, Chengbin Wu, Lin Shenwith enhanced chemiluminescence. 2.6 Confocal Microscopy for Endocytosis NCI‐H441 cells (ATCC, Cat HTB‐174) were stained with CellTrace CFSE (Invitrogen, Cat C34554, 1:2000 dilution) for plasma membranes and

(9) ATP13A2 is involved in intracellular polyamine transport in lung epithelial cellsFEBS Open BioNovember 18, 2025Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa TakanoOpti‐MEM (Thermo Fisher Scientific). NCI‐H441 cells were obtained from American Type Culture Collection (HTB‐174; ATCC, Manassas, VA, USA) and maintained as previously reported [16, 17]. Briefly, the cell line was

(10) Developing a therapeutic elastase that stimulates anti-tumor immunity by selectively killing cancer cellsCell Reports MedicineNovember 18, 2025Ravindra Gujar, Chang Cui, Maria Fumagalli, Nicole Martinez, Afshin Bahador, Alain Algazi, Kevin Harrington, Court Turner, Lev BeckerCRL-2539 B16F10,ATCC,Cat# CRL-6475 NCI-H2122,ATCC,Cat# CRL-5985 NCI-H1373,ATCC,Cat# CRL-5866 NCI-H441,ATCC,Cat# HTB-174 KYSE-410,Millipore-Sigma,Cat# 94072023 HCT116,ATCC,Cat# CCL-247 HT29,ATCC,Cat# HTB-38 A549,ATCC,Cat#

(11) A first-in-class EGFR-directed KRAS G12V selective inhibitorCancer CellOctober 13, 2025Lyla J. Stanland, Hayden P. Huggins, Snehasudha S. Sahoo, Alessandro Porrello, Yogitha Chareddy, Salma H. Azam, Jillian L. Perry, Pradeep S. Pallan, Kristina Whately, Lincy Edatt, et al.Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines NCI-H441,ATCC,Cat #HTB-174 RRID: CVCL_1561 NCI-H727,ATCC,Cat #CRL-5815 RRID: CVCL_1584 SKCO1,ATCC,Cat #HTB-39 RRID: CVCL_0626 A431,ATCC,Cat

(12) Preclinical Evaluation of DB-1419, a Novel Bifunctional and Bispecific Anti–B7-H3 × PD-L1 Antibody–Drug ConjugateClinical Cancer ResearchAugust 14, 2025Chenggang Li, Jun Yao, Junjie Yang, Yu Zhang, Yang Qiu, Zhongyuan Zhu, Haiqing Hua95111733, RRID: CVCL_1559), NCI-H1975 (ATCC, Cat# CRL-5908, RRID: CVCL_1511), NCI-H441 (ATCC, Cat# HTB-174, RRID: CVCL_1561), DMS53 (ATCC, Cat# CRL-2062, RRID: CVCL_1177), and NCI-H82 (ATCC, Cat# HTB-175, RRID:

(13) Activity in an air–liquid interface lung infection model, feasibility of inhaled delivery, and stability of cell-free supernatants from Lacticaseibacillus rhamnosus against Pseudomonas aeruginosa pulmonary infectionsFront MicrobiolAugust 5, 2025Anna Maria Piras, Marta Bianchi, Nicolò Della Bona, Brunella Grassiri, Esingül Kaya, Andrea Bertacca, Chiara Migone, Giuseppantonio Maisetta, Semih Esin, Giovanna Batoniuntil use. 2.3 ALI lung infection model The human distal lung epithelial cell line NCI-H441 (ATCC HTB-174) was routinely grown in RPMI 1640 medium (Euroclone, Pero, Milan, Italy) supplemented with 2 mM L-glutamine

(14) Comprehensive physicochemical, biophysical, and in vitro characterization of lung surfactant SP-A peptidomimeticsRSC PharmaceuticsJuly 15, 2025David Encinas-Basurto, Priya Muralidharan, M. D. Saiful Islam, Ernest L. Vallorz, Stephen M. Black, Monica Kraft, Julie G. Ledford, Heidi M. MansourResazurin assay. Pulmonary cell lines A549 (ATCC® CCL-185™), H358 (ATCC® CRL-5807™) and NCI-H441 (ATCC® HTB-174™) were purchased from the American Type Culture Collection ATCC® (Manassas, VA). As previously reported,16

(15) Establishing a Highly Accurate Circulating Tumor Cell Image Recognition System for Human Lung Cancer by Pre-Training on Lung Cancer Cell LinesCancers (Basel)July 9, 2025Hiroki Matsumiya, Kenji Terabayashi, Yusuke Kishi, Yuki Yoshino, Masataka Mori, Masatoshi Kanayama, Rintaro Oyama, Yukiko Nemoto, Natsumasa Nishizawa, Yohei Honda, et al.cell lines, PC9 (RCB4455, obtained from RIKEN BioResource Research Center, Kyoto, Japan), NCI-H441 (HTB-174, obtained from ATCC, Manassas, VA, USA), and A549 (CCL-185, obtained from ATCC), were used for pre-training.

(16) Preclinical Evaluation of DB-1419, a Novel Bifunctional and Bispecific Anti–B7-H3 × PD-L1 Antibody–Drug ConjugateClin Cancer ResJune 11, 2025Chenggang Li, Jun Yao, Junjie Yang, Yu Zhang, Yang Qiu, Zhongyuan Zhu, Haiqing Hua95111733, RRID: CVCL_1559), NCI-H1975 (ATCC, Cat# CRL-5908, RRID: CVCL_1511), NCI-H441 (ATCC, Cat# HTB-174, RRID: CVCL_1561), DMS53 (ATCC, Cat# CRL-2062, RRID: CVCL_1177), and NCI-H82 (ATCC, Cat# HTB-175, RRID:

(17) Regulation of neutrophil migration in acute pulmonary inflammation by extraneuronal α1 gamma-aminobutyric acidA receptorsCell Death DisApril 18, 2025Anika Fuhr, Robin Roediger, Mariana Simelitidis, Jutta Gamper-Tsigaras, Markus Templin, Michael Kormann, Bernd Antkowiak, Uwe Rudolph, David Köhler, Peter Rosenberger, et al.cultivated on top of a transwell insert (Corning, Corning, NY, USA), human epithelial cell (H441) (HTB-174, ATCC, USA) on the bottom. Neutrophils isolated from human whole blood migrated through the monolayer

(18) Oxidative damage to lung mitochondrial DNA is a key contributor to the development of chemical lung injuryRedox BiolMarch 29, 2025Shubham Dubey, Zhihong Yu, Emily Morgan Stephens, Ahmed Lazrak, Israr Ahmad, Saurabh Aggarwal, Shaida Andrabi, M. Iqbal Hossain, Tamas Jilling, Solana R. Fernandez, et al.in-house primers. Cell culture and treatment. Human NCI–H441 cells (Human Lung epithelial cells, Catt No. HTB-174™) were purchased from ATCC (American Type Culture Collection, USA) and cultured as described previously

(19) Innovative Dual Combination Cospray-Dried Rock Inhibitor/l-Carnitine Inhalable Dry Powder AerosolsACS Bio & Med Chem AuDecember 18, 2024Maria F. Acosta, David Encinas-Basurto, Michael D. Abrahamson, Basanth Babu Eedara, Don Hayes, Jeffrey R. Fineman, Stephen M. Black, Heidi M. Mansourlines A549 alveolar (ATCC CCL-185), H358 bronchioalveolar (ATCC CRL-5807), and NCI-H441 [H441] (ATCC HTB-174) were purchased from the American Type Culture Collection ATCC (Manassas, Virginia). Dulbecco’s modified

(20) Cross-Linked Thiolated Hydroxypropil-β-Cyclodextrin for Pulmonary Drug DeliveryInternational Journal of Molecular SciencesAugust 29, 2024Luca Cerri, Chiara Migone, Lucia Vizzoni, Brunella Grassiri, Angela Fabiano, Anna Maria Piras, Ylenia ZambitoNCI-H441 epithelial cell line was purchased from the American Type Culture Collection LGC standards ((ATCC HTB-174), Milan, Italy) and propagated as indicated by the supplier. Olive leaf extracts of the Giarraffa varieties

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NCI-H441 [H441] from ATCC

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