THLE-2 from ATCC

Product Specs
ItemTHLE-2
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-2706
TypeCell Biology
SpeciesHomo sapiens
TissueLeft lobe, Liver
Cell LineTHLE-2
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Liver
Cell Typeepithelial cell
Ageadult
ApplicationsThese immortalized human liver cells constitute an in vitro model for pharmacotoxicological studies and for the investigation of etiology and pathogenesis of human hepatocellular carcinoma. -

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Aspergillus flavus glutathione transferase as a potential approach for synthesis of epothilone-glutathione conjugates with enhanced anticancer activity for the drug-resistant cells: characterization and molecular docking analysisBMC MicrobiolApril 28, 2026Ashraf S. A. El-Sayed, Ahmed A. Shindia, Hala Ammar, Eman Fikry, Samar A. Khashana, Marwa A. Yassinconjugate against the liver carcinoma (HepG-2) (ATCC HB-8065), and normal epithelial cells (THLE-2) (ATCC CRL-2706) was determined by the MTT reagent [13]. The 96-well microtiter plate was inoculated with 105cells/well,

(2) Perfluorooctane Sulfonate (PFOS) Disrupts Mitochondrial Activity and Cell Adhesion in Liver CellsJ XenobiotApril 13, 2026Phuong D. Tran, Kyoungtae KimMaterials and Methods 2.1. Materials The HepG2 cell line (ATCC HB8065) and THLE-2 cell line (ATCC CRL-2706 TM) were obtained from American Type Culture Collection (Manassas, VA, USA). Dimethyl sulfoxide (DMSO)

(3) Preclinical Evaluation of Triptophenolide-Induced Apoptosis in Hepatoblastoma (HepG2) and Hepatocellular Carcinoma (HuH7) Cell LinesInt J Mol SciApril 3, 2026Zufa Sabeel, Ruolan Chen, Yan Liu, Xiaoyang Chen, Wenjing Zhang, Shangyang Pan, Lu Ying, Changyuan Yu, Zhao Yang(CL-0120) and HuH7 (CL-0103)) were obtained from Procell Life Sciences (Wuhan, China) and the THLE-2 (CRL-2706) normal human liver epithelial cell line was obtained from ATCC. Cells were cultured in Dulbecco’s Modified

(4) PRICE: direct and robust detection of microRNAs at single-nucleotide resolutionNature CommunicationsFebruary 12, 2026Buhua Wang, Shuai Zhou, Xi Zhang, Rui Wang, Shuo Huang, Anyi Li, Haowei He, Yingai Zhang, Yangdao Wei, Zhiqing Yang, et al.Supplementary Data 3 and Supplementary Table 9, respectively. Cell culture The THLE-2 cells (ATCC, CRL-2706 ™) were cultured in THLE-2 complete medium (purchased from ProCell), while HepG2 cells (Hzbscience,

(5) A Microfibrous Extracellular Matrix Platform for In Vitro Hepatotoxicity Screening: A Next-Generation Model for Humanized Liver ResearchACS Pharmacology & Translational ScienceJanuary 9, 2026Navneet Kaur, Mary Jean Savitsky, Michael Lipscomb, Dazhi Yang, Jamel AliLiver Cells (THLE-2) Hepatocytes Hepatotoxicity was evaluated using THLE-2 cells obtained from ATCC (CRL-2706). The cells were cultured in a collagen-coated T25 cm3 flask with the BEGM basal media supplemented

(6) Nobiletin Attenuates Inflammation and Modulates Lipid Metabolism in an In Vitro Model of Intestinal Failure-Associated Liver DiseasePharmaceuticsJanuary 9, 2026Marta Belka, Aleksandra Gostyńska-Stawna, Karina Sommerfeld-Klatta, Maciej Stawny, Violetta Krajka-Kuźniak(Saint Louis, MO, USA). 2.2. Cells and Culture Conditions Human immortalized hepatocytes, THLE-2 (ATCC CRL-2706) cells, were provided by the American Type Culture Collection (ATCC, Manassas, VA, USA). THLE-2 cells

(7) Impaired nitrogenous waste clearance promotes hepatocellular carcinomaScience AdvancesJanuary 9, 2026Xinlu Han, Jianliang Shen, Junrong Yan, Rahul Tacke, Weiwei Dai, Qingqing Mao, Heineken Queen Daguplo, Shuyang Liu, Ariful Islam, Tong Liu, et al.Culture Collection (ATCC) HB-8064], Hep G2 (ATCC, HB-8065), SK-Hep-1 (ATCC, HTB-52), THLE-2 (ATCC, CRL-2706), PLC/PRF/5 (ATCC, CRL-8024), SNU-387 (ATCC, CRL-2237), SNU-423 (ATCC, CRL-2238), SNU-449 (ATCC, CRL-2234),

(8) A novel TM4SF4-targeting therapeutic antibody candidate with antitumor activity by blocking IGF1R and CD44 signaling and downregulating PD-L1 and B7-H4TheranosticsJanuary 1, 2026Rae-Kwon Kim, Chang-Kyu Heo, Mun Ju Choi, Yeon-jee Kahm, Min Kyu Kim, Minyong Lee, Ji Yoon Lee, Hwangseo Park, Uhee Jung, Byung-Chul Shin, et al.listed in Table S1. Cell culture and transfections HEK293T (CRL-3216), A549 (CCL-185), and THLE-2 (CRL-2706) cells were obtained from ATCC, while Calu-3, Huh7, and MIA PaCa-2 cells were from KCLB. Human primary

(9) THBS1 as a candidate biomarker and fibrotic mediator in radiation-induced liver injury: insights from TMT-labeled quantitative proteomicsFrontiers in PharmacologyNovember 21, 2025Zixi Wang, Tong Wu, Haixu Wang, Yawen Deng, Jing Liu, Tingting Wang, Xue Ren, Ying Sun, Haibo Zhang, Defu Yang, et al.human hepatocyte line (THLE-2) was purchased from ATCC (Manassas, VA, United States; catalog numbers CRL-2706 and CRL-11233) and maintained in BEGM SingleQuots medium (iCell, Shanghai, China) containing 10% FBS. 2.8

(10) Lemon-derived nanovesicles facilitate trans-kingdom transfer of lncRNAs to human cellsFrontiers in Molecular BiosciencesNovember 17, 2025Vincenza Tinnirello, Roberta Gasparro, Giulia Duca, Laura Miozzi, Silvia Rotunno, Francesco Mercati, Alice Conigliaro, Riccardo Alessandro, Stefania Raimondo2023; Raimondo et al., 2015; Raimondo et al., 2022). 2.2 Cell culture The THLE-2 cell line (ATCC CRL-2706™, LGC Standards, Manassas, VA, USA) was used as an in vitro model of healthy human hepatocytes. Cells

(11) Vagal blockade of the brain-liver axis deters cancer-associated cachexiaCellOctober 16, 2025Aliesha Garrett, Naama Darzi, Ashlesha Deshmukh, Nataly Rosenfeld, Omer Goldman, Lital Adler, Elizabeta Bab-Dinitz, Oded Singer, Alireza Hassani Hassani Najafabadi, Chi Wut Wong, et al.cells. Primary Human Hepatocytes culture THLE-2, primary human hepatocytes, were purchased from ATCC (#CRL-2706). Cells were seeded in 6 or 12 wells plates with BEGM BulletKit medium (Lonza; #CC-3171 & CC-4175),

(12) Targeting oncogenic K-RAS mutants with small-molecule degrader XMU-MP-9 through NEDD4-1Acta Pharmaceutica Sinica BJuly 25, 2025Taoling Zeng, Tingting Jiang, Baoding Zhang, Ting Zhang, Wanjun Dai, Xun Yin, Yunzhan Li, Zhuoran Yu, Caiming Wu, Yaying Wu, et al.fibroblast NIH3T3 (CRL-1658), mouse embryonic fibroblast MEF (CRL-2991), and human liver epithelial THLE-2 (CRL-2706) cell lines were obtained from ATCC. Human colon cancer HT-29 (SCSP-5032), human bronchial epithelial

(13) Combination of astragalus polysaccharide with Diosbulbin B exerts an enhanced antitumor effect in BRAFmut papillary thyroid cancer with decreased liver toxicityCancer Cell IntJuly 2, 2025Shuai Xu, Qi Liang, Hang Li, Hai Zhou, Zhenyuan Xu, Yanjun Yan, Yue Zhang, Renqun Ye, Xujun Younormal liver epithelial cell line THLE-2 was purchased from the American Type Culture Culture (cat no. CRL-2706, ATCC, MA, USA) and was cultured in BEGM kit (ATCC) with extra 5 ng/mL epidermal growth factor (EGF),

(14) Identification and exploration of anticancer activity of novel peptides isolated from the edible bivalve Callista chione in hepatic and colon cancer cell linesToxicology reportsJune 1, 2025Hussein AAA, Okasha H, ElZallat M, Ghoname SI, Habib MR, Hammam OA, El-Dabaa E, Salem MB.compounds to evaluate any potential cytotoxicity on non-cancerous cells. Normal human hepatocytes (ATCC: CRL-2706) and normal kidney epithelial cells taken from an African green monkey (VERO cells, ATCC: CCL-81) were

(15) Targeting transcription factors through an IMiD independent zinc finger domainEMBO Molecular MedicineMay 14, 2025Bee Hui Liu, Miao Liu, Sridhar Radhakrishnan, Meng-Yuan Dai, Chaitanya Kumar Jaladanki, Chong Gao, Jing Ping Tang, Kalpana Kumari, Mei Lin Go, Kim Anh L Vu, et al.fitting from Prism (GraphPad). Cell culture Cell lines ((SNU398 (CRL-2233), SNU387 (CRL-2237), THLE2 (CRL-2706), THLE3 (CRL-3583), A549 (CRM-CCL-185), H1299 (CRL-5803), H838 (CRL-5844), H552 (CRL-5810), H2030 (CRL-5914),

(16) Development and Validation of In Vitro Assessment Protocol of Novel Intravenous Nanoemulsions for Parenteral NutritionPharmaceuticsApril 8, 2025Joanna Czerniel, Aleksandra Gostyńska-Stawna, Karina Sommerfeld-Klatta, Tomasz Przybylski, Violetta Krajka-Kuźniak, Maciej Stawnyto the methodology for intravenous lipid NEs outlined in our previous study [22]. The THLE-2 (ATCC CRL-2706) cells were cultured in BEGM medium supplemented with the Bullet Kit (Lonza, Cologne, Germany) and 10%

(17) Profiling of Environmental Mixtures Containing Metals for Their Toxicity Pathways and Mechanism of ActionEnvironmental Science & TechnologyMarch 18, 2025Masato Ooka, Jinghua Zhao, Li Zhang, Ruili Huang, Srilatha Sakamuru, Charlotte TeKrony, Jui-Hua Hsieh, Bradley J. Collins, June K. Dunnick, Darlene Dixon, et al.epithelial cells (ATCC, Cat No. PCS-400–012), primary liver epithelial cells (THLE-2, ATCC, Cat No. CRL-2706), primary peripheral blood mononuclear cells (ATCC, Cat No. PCS-800–011), HepG2 cell, HCT116 cells,

(18) EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus–Positive Hepatocellular Carcinoma CellsThe Kaohsiung Journal of Medical SciencesMarch 17, 2025Fang Xue, Tian‐Feng Sunthe Declaration of Helsinki. 2.2 Cells and Treatment The human liver epithelial cell line THLE‐2 (CRL‐2706) and two HBV‐positive HCC cell lines, SNU‐398 (CRL‐2233) and Hep3B (HB‐8064) were obtained from ATCC

(19) Alichondrichlorin, a Novel Chlorohydrin-Containing Natural Product With Tumoral Cytotoxic Activity Isolated From the Planctomycetota Bacterium Alienimonas chondri LzC2TMicrob BiotechnolFebruary 24, 2025Inês R. Vitorino, José D. N. Santos, Gloria Crespo, Ignacio Pérez-Victoria, Jesús Martín, Lorena Rodriguez, Maria C. Ramos, Teresa P. Martins, Pedro N. Leão, Francisca Vicente, et al.control, the compound was additionally tested against the non‐tumoural human cell line THLE‐2 (ATCC, CRL‐2706). Data resulting from the assays was interpreted and analysed using the Genedata Screener Software and

(20) ZSCAN16 expedites hepatocellular carcinoma progression via activating TBC1D31Cell divisionNovember 7, 2024Wang X, Xiao B, Zhong F, Zhou Y, Wang Q, Jiang J.ATCC) (PLC/PRF/5) supplemented with 10% FBS and 1% P/S. Transformed human liver epithelial-2 (THLE-2, CRL-2706, ATCC) was cultured in BEGM (CC-3170, Lonza, Fair Lawn, NJ, USA) supplemented with 10% FBS, 5 ng/mL

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