Fig 1: Salmonella invasion in the presence of C. albicans.a, Schematic representation of experimental setup for Invasion assay. b & c, Invasion assay of STm infected (MOI = 1) colonic epithelial cells (Caco2). STm either alone or with C. albicans ATCC(in b) C. albicans SC5314 (in c) in a 10:1 (Salmonella to C. albicans) ratio were incubated for 2 h prior to the assay. Data are geometric mean ± s.d. n = 4 independent experiments. Two-tailed unpaired t-test for comparison. d, Invasion assay of STm infected (MOI = 1) colonic epithelial cells (T84). STm either alone or with C. albicans ATCC in a 10:1(Salmonella to C. albicans) ratio or curdlan (ß-1,3-glucan) 100 μg/ml were incubated for 2 h prior to the assay. Data are geometric mean ± s.d. n = 3 independent experiments. Ordinary one-way ANOVA for comparison. e, Sedimentation assay of STm strains and C. albicans. Data are geometric mean ± s.d. n = 6 (for WT), n = 4 (for Δfim, WT+mannose, ΔinvA, ΔspiB), n = 5 (for ΔinvAΔspiB) and n = 3 (for Δbcf, +C.a. ATCC, and +C.a.529 L) independent experiments. Ordinary one-way ANOVA for comparison. Assays were performed with multiple mutants depicted in Fig. 2b and Extended Data Fig. 2e and one STm WT control in parallel. For easier comparison, STm WT and Δfim values from Fig. 2b were also plotted in this graph. f,g, Fluorescence image of STm (red) and C. albicans SC5314 (green) in vitro present as both yeast and hyphae (in f) and in presence of 5% mannose (in g). h, Invasion assay of STm infected (MOI = 1) colonic epithelial cells (Caco2). STm either alone or with C. albicans ATCC in a 10:1 (Salmonella to C. albicans) ratio with or without mannose were incubated for 2 h prior to the assay. Data are geometric mean ± s.d., n = 4 independent experiments. Two-way ANOVA for comparison. i, Invasion assay of STm infected (MOI = 1) colonic epithelial cells (Caco2). STm either alone or with the supernatant from C. albicans ATCC containing cultures were incubated for 2 h prior to the assay. Data are geometric mean ± s.d. n = 4 (for STm alone) and n = 3 (for other groups) independent experiments. Ordinary one-way ANOVA for comparison. j, Invasion assay of STm infected (MOI = 1) colonic epithelial cells (Caco2). STm either alone or with C. albicans SC5314 in a 10:1 (Salmonella to C. albicans) ratio incubated for 2 h prior to the assay. For direct, both microbes were added to host cells without pre- incubation. Data are geometric mean ± s.d. n = 4 independent experiments. Kruskal-Wallis test for comparison. Bars with no statistics have p > 0.9999. STm, Salmonella. Source data