Fig 1: The dispersion facilitates the regulation of relevant proteins associated with FAP. Digital confocal fluorescence microscopy at 60× magnification of vinculin (green) and actin (red) expression in Hs 895.T (ATCC® CRL7637™) melanoma with a predominantly mesenchymal phenotype. The images corresponding to the control and 24 h post treatment are indicated at the left- and righthand sides of the figure, respectively. The delocalization of vinculin in the cytoplasm is taken as evidence of FAK regulation in post-treatment malignant cells. The indicated bar length corresponds to 50 μm.
Fig 2: Digital confocal fluorescence microscopy at 60× magnification of vinculin (green), actin cytoskeleton (red) and focal adhesion (composite of vinculin and actin expression) in Hs 895.T (ATCC® CRL7637™) cells in (A) control and (B) 24 h post treatment. Focal adhesions were detected using an anti-vinculin monoclonal antibody and an FITC-conjugated secondary antibody; actin was detected using TRITC conjugated Phalloidin. The delocalization of vinculin in the cytoplasm is taken as evidence of FAK regulation in post-treatment malignant cells. Focal adhesion points localized on the interphase with the ECM increase, as evidenced by the bright spots on the cell membrane. Scale bar is 5 µm.
Supplier Page from ATCC for Hs 895.T