Micrococcal Nuclease from New England Biolabs

Product Specs
ItemMicrococcal Nuclease
CompanyNew England Biolabs
Price $81.00 Inquire
Catalog NumberM0247S
Quantity320000 gel units
Concentration2,000,000 gel units/ml
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
(Kunitz Unit) One unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260 nm in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dnmt1 mediates epigenetic restriction of invasive traits in clonal crayfishNat CommunMarch 26, 2026J. Jaime Diaz-Larrosa, Vitor Carneiro, Katharina Hanna, Günter Raddatz, Frank Lykoprotease inhibitor) and treated with 8 U of micrococcal nuclease (MNase) (New England Biolabs, Cat. No.: M0247S) for 15 min at 37 °C and stopped by adding 10 µL 10 x sonication buffer (Tris 10 mM, EDTA 1 mM, 0.1%

(2) m6A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo developmentThe EMBO JournalMarch 11, 2026Ruibao Su, Di Gao, Zongchang Du, Tie-Gang Meng, Chao Li, Lei-Ning Chen, Xiaoting Lin, Changchang Cao, Li-Hua Fan, Yanbin Dong, et al.immunoprecipitated RNAs were fragmented with 1 × 10−8 U micrococcal nuclease (MNase, New England BioLabs, M0247S) for 3 min at 37 °C. The fragmented RNA 3’ ends were dephosphorylated on beads using FastAP alkaline

(3) Augmented CENH3 loading is accompanied by transcriptional and epigenetic reprogramming at rice centromeres during meiosisGenome BiologyMarch 5, 2026Lei Cao, Yangzi Zhao, Hanli You, Huixin Liu, Bingxin Wang, Yonghang Run, Han Yang, Minsi Wen, Jiawei Chen, Pengtao Zhao, et al.PMCs was performed as described previously [62]. In brief, the PMC pellet was trimmed by MNase (Cat# M0247S, New England Biolabs) first for 10 min at 37 °C, and then sonicated for 10 cycles (20 s on, 40 s off)

(4) Acetylation of H3K115 is detected associated with fragile nucleosomes at CpG island promoters and active regulatory siteseLifeMarch 4, 2026Yatendra Kumar, Dipta Sengupta, Elias T Friman, Robert S Illingworth, Manon Soleil, Zheng Fan, Hua Wang, Kristian Helin, Matthieu Gerard, Wendy A Bickmorekit,SNAP-ChIP K-AcylStat Panel,Epicypher,# 19-3001, Commercial assay or kit,MNase,New England Biolabs,M0247S, Commercial assay or kit,Dynabeads Protein-A,Invitrogen,10001D, Commercial assay or kit,Tn5,Illumina,#

(5) Dysbiosis of the enteric DNA virome correlates with the development of cachexia in a murine Lewis lung carcinoma (LLC) modelArchives of VirologyMarch 1, 2026David Aciole Barbosa, Yara N.L.F. de Maria, Fabiano B. Menegidio, Regina Costa de Oliveira, Daniela L. Jabes, Luiz R. Nunestreated with benzonase (Milipore/Sigma, catalog no. E1014-5KU) and micrococcal nuclease (NEB, catalog no. M0247S) to degrade genomic material not protected by viral capsids. DNA/RNA was then extracted and purified

(6) Bromodomain protein IBD1 bridges histone acetylation and H2A.Z deposition to fine-tune transcriptionNucleic Acids ResearchFebruary 23, 2026Zhe Zhang, Haicheng Li, Aili Ju, Fei Ye, Fan Wei, Yongqiang Liu, Junhua Niu, Hongzhen Jiang, Yuanyuan Wang, Shan Gaototal of 1 × 108 macronuclei (MACs) from HA-tagged cells were digested with micrococcal nuclease (NEB, M0247S) to generate mononucleosomes. The supernatant was incubated with anti-HA magnetic beads (Thermo Scientific,

(7) The dynamic landscape and conserved regulation of the m6Am and m6A methylomes during the human and mouse oocyte-to-embryo transitionNucleic Acids ResFebruary 10, 2026Ruibao Su, Zongchang Du, Sen Li, Hanyan Liu, Di Gao, Shaoquan Zhan, Chao Li, Tie-Gang Meng, Lei-Ning Chen, Li-Hua Fan, et al.immunoprecipitated RNAs were fragmented on beads with 1 × 10−8 U micrococcal nuclease (MNase, New England BioLabs, M0247S) for 3 min at 37°C. The fragmented RNA 3′ ends were dephosphorylated using FastAP alkaline phosphatase

(8) Chromatin architecture and physical constriction cooperate in phenotype switching and cancer cell disseminationbioRxivFebruary 8, 2026Pietro Berico, Cody Dunton, Luay Almassalha, Amanda Flores Flores-Yanke, Karla I. Medina, Nicolas Acosta, Tara Muijlwijk, Catherine Do, Soobeom Lee, Sharon N Edmiston, et al.resuspended in 1 volume of sucrose buffer supplied with 1mM CaCl2 and 20U/μl of Micrococcal Nuclease (NEB, #M0247S), and incubated in thermoblock for 10min at 37°C. Micrococcal digestion was stopped by adding EDTA (final

(9) The chromatin remodeller CHD4 regulates transcription factor binding to both prevent activation of silent enhancers and maintain active regulatory elementseLifeFebruary 3, 2026Andria Koulle, Oluwaseun Ogundele, Devina Shah, India Baker, Maya Lopez, David Lando, Nicola Reynolds, Ramy Ragheb, Ernest D Laue, Brian Hendrichpaper,PRJNA1332303,https://www.ncbi.nlm.nih.gov/sra "Peptide, recombinant protein",Micrococcal nuclease,New England Biolabs,M0247S, "Peptide, recombinant protein",pAG-MNAse,PMID:31232687,,Made in-house "Peptide, recombinant protein",pA-Tn5,PMID:31036827,,Made

(10) Epigenetic Gatekeeping of Intestinal Stem Cell TransformationbioRxivFebruary 3, 2026Alireza Lorzadeh, Sweta Sharma, Geroge Ye, Sabrina Rabaya, Yun Jee Kang, Ramesh A. Shivdasani, Unmesh Jadhavcocktail. Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction. The digested chromatin

(11) Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiationbioRxivJanuary 24, 2026Sandhya Yadav, Tapan Sharma, Teresita Padilla-Benavides, Anthony N. ImbalzanoCell Signaling Technology) as recommended by the manufacturer. Chromatin was digested with 1000 U micrococcal nuclease (M0247S; New England Biolabs) for 30 min at 37°C. Reactions were stopped with 5 μL of 0.5 M EDTA. Nuclei were

(12) Efficient whole-genome sequencing of Monkeypox virus using a novel nuclease-multiple displacement amplification enrichment methodnpj VirusesJanuary 21, 2026Masayasu Misu, Takeshi Kurosu, Tomoki Yoshikawa, Madoka Kawahara, Kohei Oishi, Masayuki Shimojima, Hideki EbiharaThe supernatant was treated with the non-specific endo-exonuclease Micrococcal Nuclease (catalog No. M0247S; NEB, Ipswich, MA) to digest unwanted nucleic acids while preserving the viral DNA. The 200-μL reaction

(13) Dual genomic localizations and gene regulatory functions of MBD-2 with and without NuRD in Caenorhabditis elegans which lacks DNA methylationNature CommunicationsJanuary 20, 2026Hok Ning Tsui, Charmaine Yan Yu Wong, Chaogu Zheng, Karen Wing Yee Yuenrinsed twice with ice-cold PBS supplemented with proteinase inhibitor, once with MNase buffer (NEB Cat# M0247S). The lysis and release of chromatin from worms were performed according to Tabuchi et al.86 with slight

(14) The ER membrane protein complex acts as a chaperone to promote the biogenesis of multi-bundle membrane proteinsbioRxivJanuary 19, 2026Marinda Stanton, Bharti Singal, Mahamaya Biswal, Megha Agarwal, Caroline Elizabeth Scheuing, Gerardo Dasaev Vargas, Alex Gao, Casey A Gifford, Tino PleinerSucrose). To remove endogenous mRNAs, membranes were further treated with 4U/μL Micrococcal nuclease (#M0247S, New England Biolabs, USA) in the presence of 1 mM CaCl2, 2 U/mL TurboDNAse (#AM2238, Thermo Fisher Scientific

(15) Effector gene silencing coordinated by histone methylation and small RNAs enhances host adaptation in a plant pathogenNucleic Acids ResearchJanuary 8, 2026Liyuan Wang, Xuewei Xiang, Guoyu Yin, Haidong Shu, You Wu, Han Chen, Ren Na, Mark Gijzen, Yingnan Hou, Suomeng Dongwas gently resuspended in 1 ml of MNB buffer. Chromatin digestion was carried out using MNase (NEB, M0247S) at 37°C for 10 min. The reaction was terminated by adding 10% volume of 0.5 M EDTA (ethylenediaminetetraacetic

(16) H3K27me3 ‐Mediated Epigenetic Silencing of FgHMG1 Enables Fungal Host Immune EvasionPlant Biotechnology JournalJanuary 6, 2026Xiaozhen Zhao, Bingqin Yuan, Peixue Ma, Yajie Cai, Yan Huang, Yaxuan Wang, Zhen Cheng, Yuan Chen, Minghong Zheng, Ran Zhang, et al.harvested for nuclei isolation. Chromatin was digested using 15 μL micrococcal nuclease (MNase; NEB M0247S) at 37°C for 4 min, followed by immediate termination with 250 μL 0.5 M EDTA. After centrifugation,

(17) A Pan‐TE Map Reveals the Important Role of Transposable Elements in Gene Expression and Phenotypic Diversity in 2,311 Rapeseed AccessionsAdvanced ScienceJanuary 1, 2026Zhiquan Yang, Haiyan Fan, Yifan Chen, Jiawei Li, Minjian Chen, Yingying Tan, Liang Guo, Jintao Li, Kede Liu, Qing‐Yong Yang× 10−3 m MgCl2). Next, 100 µL of the nuclei was digested using 1 µL of (2 × 103 gel U µL−1) MNase (M0247S, New England Biolabs, USA) at 37 °C for 10 min. MNase was inactivated by the addition of 0.5 m EDTA

(18) Phosphoproteomics of aged insulin-resistant bone identifies P70S6K phosphorylation of AFF4 as a gene-specific transcriptional regulatorNature CommunicationsDecember 31, 2025Mriga Dutt, Luoping Liao, Hani Jieun Kim, Ronnie Blazev, Audrey Chan, Hitesh Kore, Ayenachew Bezawork-Geleta, Li Dong, Isela Sarahi Rivera, Natalie K. Y. Wee, et al.containing 5 mM calcium chloride and treated with 4000U of Micrococcal Nuclease (New England Biolabs; M0247S) for 5 min at 37 °C. The reaction was stopped with a final concentration of 10 mM EGTA and Triton-X100

(19) Direct measurement of sub-kilobase chromatin structure reveals that linker histone H1 broadly compacts chromatin, with differential impact amongst epigenetic statesbioRxivDecember 26, 2025Hera Canaj, Irene Duba, Andres Mansisidor, Andrew Scortea, Ryan Johnson, Hugo Pinto, Arnold Ou, Nicole Pagane, Juhee Pae, Dmitry Fyodorov, et al.20 minutes, spinning down and resuspending nuclei in MB1, then adding varying volumes of MNase (NEB M0247S) to aliquots. Typically, volumes between 2 and 6 uL of 1:10 diluted MNase produced the desired level

(20) Germline epigenome editing identifies H3K9me3 as a mediator of intergenerational DNA methylation recovery in miceNat CommunDecember 25, 2025Takuro Horii, Sumiyo Morita, Shinjiro Hino, Yuko Hino, Hiroto S. Fukushima, Ryosuke Kobayashi, Mika Kimura, Mitsuyoshi Nakao, Yoichi Mizukami, Azusa Inoue, et al.and 1 mM phenylmethanesulfonyl fluoride on ice. Chromatin was fragmented in situ using 2 μ/μl MNase (M0247S, NEB) at 37 °C for 7.5 min; the reaction was stopped by adding 1/10 volume of 100 mM EDTA and diluted

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