MfeI-HF from New England Biolabs

Product Specs
ItemMfeI-HF
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR3589S
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C
Concentration20,000 units/ml

Sequence

C⇓AATTG
GTTAA⇑C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networksCell ReportsMarch 24, 2026Lin Lin, Naoto Kubota, Yi-Li Lam, Michelle Mingxue Song, Min Zhang, Sika Zheng2-step PCR cloning techniques using the NheI (New England Biolabs R3131S) and MfeI (New England Biolabs R3589S) cut sites. Primers used for the first round of PCR were 5′- GATGACGATAAGGCGGAGAGCTGGCTG-3′ and 5′-TAGCTAGCTTACAA

(2) RNA localization to nuclear speckles follows splicing logicNucleic Acids ResMarch 6, 2026Li Wen, Mauricio A Arias, Xinqi Fan, Joyce Xie, Sneha Paul, Susan E Liao, Marek Sobczyk, Oded Regev, Jingyi Feiproportionally based on the surface area. For linearization, circular plasmids were digested by MfeI-HF(NEB, R3589S) or PciI (NEB, R0665S) and then purified by the GeneJET PCR Purification Kit (Thermo Fisher, K0701).

(3) An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencingSTAR ProtocolsMarch 1, 2026Kamil Więcek, Janusz Wiśniewski, Heng-Chang Chen,New England Biolabs,M0303S HpyCH4III,New England Biolabs,R0618S BplI,Thermo Fisher Scientific,ER1311 MfeI-HF,New England Biolabs,R3589S Hpy166II,New England Biolabs,R0616S Agarose powder,Thermo Fisher Scientific,Cat. No. 16500500 T7 RNA polymerase

(4) Production of Genetically Engineered Extracellular Vesicles for Targeted Protein DeliveryBIO-PROTOCOLNovember 5, 2025Leyla A. Ovchinnikova, Evgeniy G. Evtushenko, Dmitriy V. Bagrov, Anton O. Goncharov, Alexander G. Gabibov, Yakov A. Lomakinendonuclease BstBI (NEB, catalog number: R0519S) 28. Restriction endonuclease MfeI-HF (NEB, catalog number: R3589S) 29. rhGM-CSF (Sci-Store, catalog number: PSG030-10) 30. rhIL-4 (Sci-Store, catalog number: PSG040-10) 31.

(5) Enhancing cap-independent translation of linear mRNANat CommunOctober 16, 2025Sebastian Golojuch, Brendan Largey, Afaf H. El-Sagheer, Tom Browndigesting circRNA-synIRES-R25-mNeonGreen plasmid with BamHI-HF (#R3136S, New England Biolabs) and MfeI-HF (#R3589S, New England Biolabs) restriction enzymes following the manufacturer’s protocol and subsequently separated

(6) Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technologyNat CommunJuly 18, 2025De Xing, Tao Bai, Ozlem Neyisci, Seyedeh Zahra Paylakhi, Alexander J. Duval, Yasemin Tekin, Mazhar AdliCMV-OsTIR1F74G) (Addgene,140536) backbone plasmid, digesting with NdeI and MfeI-HF (New England Biolabs, R0111S, R3589S), followed by removing DNA sequence between two loxP sites with Cre Recombinase (New England Biolabs,

(7) Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decayCell GenomicsJuly 9, 2025Divya Kolakada, Rui Fu, Nikita Biziaev, Alexey Shuvalov, Mlana Lore, Amy E. Campbell, Michael A. Cortázar, Marcin P. Sajek, Jay R. Hesselberth, Neelanjan Mukherjee, et al.NMD– reporters for each sequence, each EJC-independent reporter was digested with EcoRI and MfeI (NEB, R3589S), the sticky ends were filled in using Klenow Fragment (NEB, M0212S) and the blunt ends were ligated

(8) Large-scale experimental assessment of variant effects on the structure and function of the citrate transporter SLC13A5Sci AdvJune 27, 2025Wen-An Wang, Evandro Ferrada, Christoph Klimek, Tanja Osthushenrich, Aidan MacNamara, Tabea Wiedmer, Giulio Superti-Furgainto the attB_Strp_HA_Ct_SLC13A5_Library_BC1 plasmid with Kas I and Mfe I–HF (New England Biolabs, R3589S) double digestion and T4 ligation (New England Biolabs, M0202S). The final attB_Strp_HA_Ct_SLC13A5_Library_BC1_BC2

(9) Structural basis of RNAPII transcription on the nucleosome containing histone variant H2A.BThe EMBO JournalMay 30, 2025Munetaka Akatsu, Rina Hirano, Tomoya Kujirai, Mitsuo Ogasawara, Haruhiko Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi KurumizakaDNase I,Fujifilm Wako,Cat#043-26773 Ban I,New England Biolabs,Cat# R0118L MfeI-HF,New England Biolabs,Cat#R3589S Software,Software,Software Serial EM ver. 3,"Mastronarde, 2005",https://bio3d.colorado.edu/SerialEM/ Relion

(10) Comprehensive benchmarking of genome editing quantification methods for plant applicationsiScienceApril 29, 2025Zheng Gong, Yan Zhang, Di Xia, Sohye Yoon, Peter Alexander Crisp, José Ramón Botella,New England Biolabs,R0542S BsrDI,New England Biolabs,R0574S BglII,New England Biolabs,R0144S MfeI-HF,New England Biolabs,R3589S RsaI,New England Biolabs,R0167S BsrDI,New England Biolabs,R0574S HpaI,New England Biolabs,R0105S BstNI,New England Biolabs

(11) Serologic Cross-Reactivity between the Mumps Virus Vaccine Genotype A Strain and the Circulating Genotype G StrainVirusesSeptember 8, 2024Sabaparvin Shaikh, Michael Carpenter, Lisa Lin, Jasmine Rae Frost, Elizabeth McLachlan, Derek Stein, Paul Van Caeseele, Alberto Severiniisolated using the QIAprep Miniprep kit (Qiagen, cat#27104). Restriction digestion with MfeI (NEB, cat#R3589S) and EcoRI (NEB, cat#R0101S) and agarose gel analysis were performed to ensure that the fragment sizes

(12) A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decayNucleic Acids ResearchApril 12, 2024Divya Kolakada, Amy E Campbell, Laura Baquero Galvis, Zhongyou Li, Mlana Lore, Sujatha JagannathanKit,M2200L,"New England Biolabs (NEB), Massachusetts, USA" EcoRI-HF,R3101S,"NEB, Massachusetts, USA" MfeI-HF,R3589S,"NEB, Massachusetts, USA" AgeI,R3552S,"NEB, Massachusetts, USA" Klenow Fragment,M0212S,"NEB, Massachusetts,

(13) Non-viral nitric oxide-based gene therapy improves perfusion and liposomal doxorubicin sonopermeation in neuroblastoma modelsTheranosticsJune 4, 2023Aditi Bellary, Chance Nowak, Isabella Iwanicki, Fernando Flores-Guzman, Lydia Wu, Jessica J. Kandel, Theodore W. Laetsch, Leonidas Bleris, Sonia L. Hernandez, Shashank R. Sirsitranscript and the mKate2 expression vector was digested with AgeI-HF (NEB, #R3552S) and MfeI-HF (NEB, #R3589S). The iNOS transcript was cloned into the expression vector with T4 DNA Ligase (NEB, #M0202S), replacing

(14) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozziverification, plasmids were digested with BamHI-HF (New England Biolabs R3136S)/MfeI-HF (New England Biolabs R3589S) (gp130), MfeI-HF/AgeI-HF (New England Biolabs R3552S) (LIFR), AgeI-HF/PsiI-v2 (Thermo Fisher Scientific

(15) The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogasteriScienceNovember 18, 2022Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik HedenstrÃ¶m, BjÃ¶rn Bohman, Marie Bengtsson, Paul G. Becher, et al.gene-specific primers (Table S2), ligated with T4-DNA ligase (#M1804, Promega, Madison, USA) either MfeI/NotI (#R3589S/ # R0189S, New England Biolabs, Ipswich, UK) or EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) into

(16) Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsioneLifeApril 12, 2021Chaudhari K, Gorla M, Chang C, Kania A, Bashaw GJ.drug",BbsI,New England Biolabs,Cat#R3539S, "Chemical compound, drug",MfeI,New England Biolabs,Cat#R3589S, "Chemical compound, drug",Proteinase K,Roche Diagnostics,Cat#03115828001, "Chemical compound, drug",Protein

(17) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. WalterR0631S Nt.BbvCI,NEB,Cat# R0632S Nt.BspQI,NEB,Cat# R0644S AflII,NEB,Cat# R0520S NcoI,NEB,Cat# R0193L MfeI-HF,NEB,Cat# R3589S "Klenow Fragment, exo-",NEB,Cat# M0212S AlexaFluor647-maleimide,Thermo Fisher,Cat# A20347 SYTOX Green,Thermo Fisher

(18) Simultaneous Examination of Cellular Pathways using Multiplex Hextuple Luciferase AssayingCurrent Protocols in Molecular BiologyJune 26, 2020Alejandro Sarrion‐Perdigones, Lyra Chang, Yezabel Gonzalez, Tatiana Gallego‐Flores, Damian W. Young, Koen J.T. Venkencat. no. R3142S), BsmBI (New England Biolabs, cat. no. R0580S), MfeI-HF (New England Biolabs, cat. no. R3589S), NcoI-HF (New England Biolabs, cat. no. R3193S), and XhoI (New England Biolabs, cat. no. R0146S). Restriction

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MfeI-HF

MfeI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (18)

Reviews

MfeI-HF from New England Biolabs

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