Human Methylcellulose Enriched Media from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Methylcellulose Enriched Media
CompanyR&D Systems, a Bio-Techne Brand
Price $546.00Supplier Page View Company Product Page
Catalog NumberHSC005
Quantity100 mL
TypeMedia
SpeciesHuman
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 100 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The dark side of the light (UVA): melanoma microenvironment and cell survival strategiesCell death discoveryOctober 20, 2025Basu A, Thorsten P, Schumacher B, Kletsas D, Scharffetter-Kochanek K.further in equal proportions in Human methylcellulose with complete cytokine media (IL-6 20 ng/mL, HSC005, R&D system) shows that GDF-15KDN shows poor survival as compared to GDF-15WT or GDF-15TG. IL-6 in the

(2) Suppressing t(4;11) Acute Leukemia by Lipopolymer Nanoparticle Delivery of siRNA Targeting KMT2A::AFF1 with Enhanced Extrahepatic DeliveryAdvanced Healthcare MaterialsJuly 21, 2025Mohammad Nasrullah, Remant KC, Amarnath Praphakar Rajendran, Saba Abbasi Dezfouli, Cezary Kucharski, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, Olaf Heidenreich, Hasan Uludağ1500 RS4;11 cells and 1000 SEM cells were seeded in 500 µL of human methylcellulose base media (Cat. #HSC005, R&D Systems, MN, USA), and the mixture was transferred into the inner wells of a 24‐well plate. To

(3) Multi-selective RAS(ON) Inhibition Targets Oncogenic RAS Mutations and Overcomes RAS/MAPK-Mediated Resistance to FLT3 and BCL2 Inhibitors in Acute Myeloid LeukemiabioRxivJune 17, 2025Bogdan Popescu, Matthew F. Jones, Madison Piao, Elaine Tran, Andrew Koh, Isabelle Lomeli, Cheryl A.C. Peretz, Natalia Murad, Sydney Abelson, Carolina Morales, et al.methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using

(4) Lipopolymers as the Basis of Non-Viral Delivery of Therapeutic siRNA Nanoparticles in a Leukemia (MOLM-13) ModelBiomoleculesJanuary 13, 2025Panadda Yotsomnuk, Amarnath Praphakar Rajendran, Daniel Nisakar Meenakshi Sundaram, Luis Carlos Morales, Cezary Kucharski, Mohammad Nasrullah, Wanwisa Skolpap, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, et al.Human methylcellulose base media (Cat. No. HSC002) and human methylcellulose enriched media (Cat. No. HSC005) for the human colony-forming cell (CFC) assay were supplied by R&D systems, Inc. (Oakville, ON, Canada).

(5) Streptavidin-drug conjugates streamline optimization of antibody-based conditioning for hematopoietic stem cell transplantationbioRxiv : the preprint server for biologyFebruary 12, 2024Yelamali AR, Chendamarai E, Ritchey JK, Rettig MP, DiPersio JF, Persaud SP.was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly. Finally, 1.1 mL of this methylcellulose suspension was plated

(6) Arginine dependency is a therapeutically exploitable vulnerability in chronic myeloid leukaemic stem cellsEMBO reportsOctober 9, 2023Kevin M Rattigan, Martha M Zarou, Zuzana Brabcova, Bodhayan Prasad, Désirée Zerbst, Daniele Sarnello, Eric R Kalkman, Angela Ianniciello, Mary T Scott, Karen Dunn, et al.from each condition were transferred to methylcellulose enriched with human cytokines (Bio‐Techne Cat# HSC005) in duplicate, and colonies were manually counted after 12–14 days. Cell lines were cultured in Plasmax

(7) LNS8801 inhibits Acute Myeloid Leukemia by Inducing the Production of Reactive Oxygen Species and Activating the Endoplasmic Reticulum Stress PathwayCancer Res CommunAugust 18, 2023Inyoung Lee, Miriam Doepner, Jillian Weissenrieder, Ariana D. Majer, Sophia Mercado, Angela Estell, Christopher A. Natale, Pamela J. Sung, J. Kevin Foskett, Martin P. Carroll, et al.104–4 × 104 cells were plated in 35 mm plates in Human Methylcellulose Enriched Media (R&D Systems, HSC005) with DMSO or 250 nmol/L LNS8801. Plates were incubated for 14 days at 37°C with 5% CO2 according to

(8) LNS8801 inhibits Acute Myeloid Leukemia by Inducing the Production of Reactive Oxygen Species and Activating the Endoplasmic Reticulum Stress PathwayCancer Research CommunicationsAugust 18, 2023Inyoung Lee, Miriam Doepner, Jillian Weissenrieder, Ariana D. Majer, Sophia Mercado, Angela Estell, Christopher A. Natale, Pamela J. Sung, J. Kevin Foskett, Martin P. Carroll, et al.104–4 × 104 cells were plated in 35 mm plates in Human Methylcellulose Enriched Media (R&D Systems, HSC005) with DMSO or 250 nmol/L LNS8801. Plates were incubated for 14 days at 37°C with 5% CO2 according to

(9) Small-Molecule Inhibition of the Acyl-Lysine Reader ENL as a Strategy against Acute Myeloid LeukemiaCancer discoveryNovember 2, 2022Liu Y, Li Q, Alikarami F, Barrett DR, Mahdavi L, Li H, Tang S, Khan TA, Michino M, Hill C, et al.containing DMSO or TDI-11055 and added to 1.5 mL human methylcellulose-enriched media (R&D Systems, #HSC005). The number of colonies was determined after incubation in a 37°C incubator for ∼2 weeks. Flow-Cytometric Analysis

(10) Direct targeted therapy for MLL‐fusion‐driven high‐risk acute leukaemiasClinical and Translational MedicineJune 22, 2022Sandra Cantilena et al.cultured for 2 weeks in HSC002 (R&D system). CD34+ cord blood–derived cells were plated and cultured in HSC005 (R&D system) for 2 weeks. Cells were cultured for two additional days in the presence of 2‐(P‐iodophenyl)‐3‐(p‐nitrophenyl)‐5‐phenyl

(11) KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression ProgramsCancer discoveryMarch 1, 2022Yan F, Li J, Milosevic J, Petroni R, Liu S, Shi Z, Yuan S, Reynaga JM, Qi Y, Rico J, et al.cells: to make 100mL complete methylcellulose media, 75mL methylcellulose base media (R&D Systems #HSC005) was supplemented with IMDM (Gibco, 12440–053), 10% FBS (Life Technologies, 16000044), 1% Penicillin

(12) CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapyJCI insightAugust 23, 2021Kim MY, Cooper ML, Jacobs MT, Ritchey JK, Hollaway J, Fehniger TA, DiPersio JF.detailed below. A small fraction of cells was plated on Human Methylcellulose Enriched Media (R&D Systems, HSC005), and colonies were harvested after 14–16 days. DNA was extracted for evaluation of CD7 gene mutations

(13) Enriching for human acute myeloid leukemia stem cells using reactive oxygen species-based cell sortingSTAR ProtocolsMarch 19, 2021Brett M. Stevens, Cristiana Oâ€™Brien, Craig T. Jordan, Courtney L. JonesDNase I,Sigma-Aldrich,4536282001 DAPI,Sigma-Aldrich,D9542-5MG CellRox deep red,thermosphere,C10422 Human methylcellulose,R&D Systems,HSC005 Busulfan,Sigma-Aldrich,B2635-25G NaCl,Sigma-Aldrich,S7653-250G ,, Experimental models: organisms/strains,Experimental models

(14) Therapeutic delivery of siRNA with polymeric carriers to down-regulate STAT5A expression in high-risk B-cell acute lymphoblastic leukemia (B-ALL)PloS oneJanuary 1, 2021Mohseni M, Kucharski C, K C RB, Nasrullah M, Jiang X, Uludağ H, Brandwein J.New England Biolabs 1 (NEB), Inc. (Ipswich, MA, USA). Human methylcellulose enriched media (Cat. No. HSC005) and human methylcellulose base media (Cat. No. HSC002) were supplied by R&D systems, Inc. (Oakville, ON,

(15) Sleeping Beauty-Mediated Drug Resistance Gene Transfer in Human Hematopoietic Progenitor CellsHuman Gene TherapyOctober 1, 2015Kendra A. Hyland, Erik R. Olson, R. Scott McIvordays in liquid culture, CD34 + cells were plated in methylcellulose medium containing human cytokines (HSC005; Bio-techne) in the SB-MEDIATED DRUG RESISTANCE GENE TRANSFER IN HUMAN HPCs 659 transposon integrants

(16) The DPY30 subunit in SET1/MLL complexes regulates the proliferation and differentiation of hematopoietic progenitor cellsBloodSeptember 25, 2014Yang Z, Augustin J, Chang C, Hu J, Shah K, Chang CW, Townes T, Jiang H.

(17) Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosaThe Journal of investigative dermatologyMay 1, 2014Tolar J, McGrath JA, Xia L, Riddle MJ, Lees CJ, Eide C, Keene DR, Liu L, Osborn MJ, Lund TC, et al.of the cell suspension was added to 4 mL aliquots of Human Methylcellulose Enriched Medium (catalog #HSC005, R&D Systems). The suspension was equally divided and placed in three 35 mm tissue culture plates and

(18) Simple and efficient methods for enrichment and isolation of endonuclease modified cellsPloS oneJanuary 1, 2014Moriarity BS, Rahrmann EP, Beckmann DA, Conboy CB, Watson AL, Carlson DF, Olson ER, Hyland KA, Fahrenkrug SC, McIvor RS, et al.(clone 4H11, eBioscience). CD34+ cells were plated in methylcellulose medium containing human cytokines (HSC005, R&D Systems) in the presence and absence of 5 µg/ml 6-thioguanine (Sigma) or 100 nM methotrexate (Bedford

(19) Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome)BloodJanuary 20, 2011Tolar J, Park IH, Xia L, Lees CJ, Peacock B, Webber B, McElmurry RT, Eide CR, Orchard PJ, Kyba M, et al.

(20) Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable deviceBritish journal of haematologyMarch 1, 2008Wojciechowski JC, Narasipura SD, Charles N, Mickelsen D, Rana K, Blair ML, King MR.μg/ml) and screened for viable haematopoietic progenitors using a colony-forming cell (CFC) assay (HSC005, R&D Systems). Briefly, following positive trypan blue staining of a separate fraction, 105 cells in

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