Human Methylcellulose Base Media from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Methylcellulose Base Media
CompanyR&D Systems, a Bio-Techne Brand
Price $225.00Supplier Page View Company Product Page
Catalog NumberHSC002
Quantity90 mL
TypeMedia
SpeciesHuman
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 90 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) FTO degrader impairs ribosome biogenesis and protein translation in acute myeloid leukemiaScience advancesAugust 15, 2025Li W, Zhao Y, Wu D, Chen Z, Qing Y, Yang F, Ji F, Zhang L, Sau L, Chen J, et al.

(2) Lipopolymers as the Basis of Non-Viral Delivery of Therapeutic siRNA Nanoparticles in a Leukemia (MOLM-13) ModelBiomoleculesJanuary 13, 2025Panadda Yotsomnuk, Amarnath Praphakar Rajendran, Daniel Nisakar Meenakshi Sundaram, Luis Carlos Morales, Cezary Kucharski, Mohammad Nasrullah, Wanwisa Skolpap, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, et al.dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human methylcellulose base media (Cat. No. HSC002) and human methylcellulose enriched media (Cat. No. HSC005) for the human colony-forming cell (CFC)

(3) Evi1 governs Kdm6b-mediated histone demethylation to regulate the Laptm4b-driven mTOR pathway in hematopoietic progenitor cellsJournal of Clinical InvestigationDecember 16, 2024Qiong Wu, Chunjie Yu, Fang Yu, Yiran Guo, Yue Sheng, Liping Li, Yafang Li, Yutao Zhang, Chao Hu, Jue Wang, et al.shRNAs or scramble, 500 cells were plated in triplicate in human Methylcellulose Base Media (R&D Systems HSC002) and scored 10 days after plating. For the treatment assay, 500 cells were plated in triplicate in human Methylcellulose Base Media

(4) Evi1 governs Kdm6b-mediated histone demethylation to regulate the Laptm4b-driven mTOR pathway in hematopoietic progenitor cellsJ Clin InvestDecember 16, 2024Qiong Wu, Chunjie Yu, Fang Yu, Yiran Guo, Yue Sheng, Liping Li, Yafang Li, Yutao Zhang, Chao Hu, Jue Wang, et al.shRNAs or scramble, 500 cells were plated in triplicate in human Methylcellulose Base Media (R&D Systems HSC002) and scored 10 days after plating. For the treatment assay, 500 cells were plated in triplicate in human Methylcellulose Base Media

(5) Arginine dependency is a therapeutically exploitable vulnerability in chronic myeloid leukaemic stem cellsEMBO reportsOctober 9, 2023Kevin M Rattigan, Martha M Zarou, Zuzana Brabcova, Bodhayan Prasad, Désirée Zerbst, Daniele Sarnello, Eric R Kalkman, Angela Ianniciello, Mary T Scott, Karen Dunn, et al.Scientific Cat# A33820‐01) and 1% penicillin/streptomycin and base methylcellulose (Bio‐Techne Cat# HSC002) used for colony‐forming cell (CFC) assays. CTV, RNASE‐PI and apoptosis CellTrace™ Violet Cell Proliferation Kit, for flow cytometry

(6) Direct targeted therapy for MLL‐fusion‐driven high‐risk acute leukaemiasClinical and Translational MedicineJune 22, 2022Sandra Cantilena et al.protocol. 2.5 Colony forming assays Human leukaemic cell lines were plated and cultured for 2 weeks in HSC002 (R&D system). CD34+ cord blood–derived cells were plated and cultured in HSC005 (R&D system) for 2 weeks.

(7) The P2X4 purinergic receptor has emerged as a potent regulator of hematopoietic stem/progenitor cell mobilization and homing-a novel view of P2X4 and P2X7 receptor interaction in orchestrating stem cell traffickingLeukemiaJanuary 1, 2022Adamiak M, Bujko K, Thapa A, Pensato V, Brzezniakiewicz-Janus K, Ratajczak J, Davies DL, Ulrich H, Kucia M, Ratajczak MZ.

(8) Danger-associated molecular pattern molecules take unexpectedly a central stage in Nlrp3 inflammasome-caspase-1-mediated trafficking of hematopoietic stem/progenitor cellsLeukemiaSeptember 1, 2021Thapa A, Adamiak M, Bujko K, Ratajczak J, Abdel-Latif AK, Kucia M, Ratajczak MZ.

(9) Therapeutic delivery of siRNA with polymeric carriers to down-regulate STAT5A expression in high-risk B-cell acute lymphoblastic leukemia (B-ALL)PloS oneJanuary 1, 2021Mohseni M, Kucharski C, K C RB, Nasrullah M, Jiang X, Uludağ H, Brandwein J.Human methylcellulose enriched media (Cat. No. HSC005) and human methylcellulose base media (Cat. No. HSC002) were supplied by R&D systems, Inc. (Oakville, ON, Canada). Trizol used for total RNA extraction and

(10) SOX11 promotes epithelial/mesenchymal hybrid state and alters tropism of invasive breast cancer cellseLifeSeptember 10, 2020Oliemuller E, Newman R, Tsang SM, Foo S, Muirhead G, Noor F, Haider S, Aurrekoetxea-Rodríguez I, Vivanco MD, Howard BA.,NeuroCult SM1 without vitamin A,StemCell Technologies,Cat # 05731, "Chemical compound, drug",Methylcellulose,R&D Systems,Cat # HSC002, "Chemical compound, drug",Cell titer-Glo,Promega,Cat # G7572, "Chemical compound, drug","Collagen

(11) The novel Isatin analog KS99 targets stemness markers in acute myeloid leukemiaHaematologicaMarch 1, 2020Annageldiyev C, Gowda K, Patel T, Bhattacharya P, Tan SF, Iyer S, Desai D, Dovat S, Feith DJ, Loughran TP Jr, et al.

(12) Non-NAD-like PARP-1 inhibitors in prostate cancer treatmentBiochemical pharmacologySeptember 1, 2019Karpova Y, Wu C, Divan A, McDonnell ME, Hewlett E, Makhov P, Gordon J, Ye M, Reitz AB, Childers WE, et al.Clonogenic Cell Survival Assay and Western blot. Cells were plated in Methylcellulose-based Media (HSC002, R&D Systems, USA), supplemented with L-glutamine and penicillin and streptomycin (10378–016, Life Technologies

(13) Specific inhibition of DPY30 activity by ASH2L-derived peptides suppresses blood cancer cell growthExperimental Cell ResearchSeptember 1, 2019Kushani K. Shah, Robert H. Whitaker, Theodore Busby, Jing Hu, Bi Shi, Zhenjia Wang, Chongzhi Zang, William J. Placzek, Hao Jiangpreviously described (17). A total of 10,000 MOLM-13 cells were plated in human methylcellulose base media (HSC002; R&D Systems) with no cytokines, containing indicated peptide at 100 μM. Proliferation and apoptosis

(14) Poly(ADP-ribose) polymerase 1 is necessary for coactivating hypoxia-inducible factor-1-dependent gene expression by Epstein-Barr virus latent membrane protein 1PLoS pathogensNovember 1, 2018Hulse M, Caruso LB, Madzo J, Tan Y, Johnson S, Tempera I.with 2.5 μM olaparib for 96 hrs, were seeded in 1.4% human methylcellulose media (R and D Systems cat HSC002) and incubated for 14 days at 37˚C. Cell proliferation assay Cells were re-suspended in PBS and incubated

(15) Multiple myeloma cells adapted to long-exposure of hypoxia exhibit stem cell characters with TGF-β/Smad pathway activationBiochemical and biophysical research communicationsFebruary 5, 2018Nakagawa Y, Ashihara E, Yao H, Yokota A, Toda Y, Miura Y, Nakata S, Hirai H, Maekawa T.

(16) Upregulation of CD11b and CD86 through LSD1 inhibition promotes myeloid differentiation and suppresses cell proliferation in human monocytic leukemia cellsOncotargetOctober 17, 2017Jianwu Fang et al.with compounds of the required dose for 10–21 days according to the manufacture’s recommendation (Cat.no.HSC002, R&D systems, Minneapolis, MN, USA). Cells were fixed and stained with Iodonitrotetrezolium chloride

(17) Mobilization studies in mice deficient in sphingosine kinase 2 support a crucial role of the plasma level of sphingosine-1-phosphate in the egress of hematopoietic stem progenitor cellsOncotargetSeptember 12, 2017Adamiak M, Chelvarajan L, Lynch KR, Santos WL, Abdel-Latif A, Ratajczak MZ.

(18) The DPY30 subunit in SET1/MLL complexes regulates the proliferation and differentiation of hematopoietic progenitor cellsBloodSeptember 25, 2014Yang Z, Augustin J, Chang C, Hu J, Shah K, Chang CW, Townes T, Jiang H.

(19) Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c miceLaboratory animal researchMarch 1, 2014Lee H, Che JH, Oh JE, Chung SS, Jung HS, Park KS.

(20) STAT3 mediates oncogenic addiction to TEL-AML1 in t(12;21) acute lymphoblastic leukemiaBloodJuly 25, 2013Mangolini M, de Boer J, Walf-Vorderwülbecke V, Pieters R, den Boer ML, Williams O.

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