MboI from New England Biolabs

Product Specs
ItemMboI
CompanyNew England Biolabs
Price $329.00 Inquire
Catalog NumberR0147L
Quantity2500 units
Concentration5-25 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

/GATC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) LINC02470 impairs natural killer cell cytotoxicity by epigenetically targeting the natural cytotoxicity triggering receptor 1Front ImmunolApril 16, 2026Xiumin Shi, Lei Zhou, Min Li, Xue Wen, Yongchong Chen, Chao Niu, Qiliang Yin, Haofan Jin, Andrew R. Hoffman, Jiuwei Cui, et al.and glycine was then used for quenching. Lysis buffer was used to lyse cells, followed by MboI (NEB, R0147) digestion at 37°C for at least 2 h. T4 DNA ligase (NEB, M0202) was used for ligation. Proteinase K

(2) In situ transplantation and multiple omics reveal holobiont adaptation in deep-sea mussel Gigantidas haimaensisiScienceFebruary 20, 2026Tong Wei, Guoyong Yan, Maeva Perez, Xing He, Wai Chuen Wong, Ting Xu, Yi Lan, Jin Sun, Pei-Yuan QianScientific,Cat#PI87787 NEB Buffer,New England Biolabs,Cat#B6003S MboI Restriction Enzyme,New England Biolabs,Cat#R0147S Sodium Dodecyl Sulfate (SDS),Thermo Scientific,Cat#28312 Biotin-14-dCTP,Thermo Fisher Scientific,Cat#19518018 Proteinase K Solution

(3) Repeat expansions in C9orf72 rewire the 3D chromatin landscape in ALSbioRxivFebruary 14, 2026Tatiana Ulloa Avila, Jingying Wang, Lydia Adams, Ellen Hu, Alvaro A. Beltran, Alexey Kozlenkov, Shreyan Urhekar, Ariana B. Marquez Gonzalez, Hun-Goo Lee, J. Mauro Calabrese, et al.formaldehyde to preserve chromatin interactions. Crosslinked chromatin was then digested with MboI (NEB, R0147S), followed by end repair, biotin labeling, and proximity-based ligation. The resulting DNA was sheared

(4) NSD3 stabilizes nuclear compartmentalization and promotes megabase-scale chromatin interactionsbioRxivFebruary 14, 2026Yi-Hung Chen, John R. Collette, Krupa Sampat, Ivaylo D. Yonchev, Catherine E. Hawkins, Christopher Ponne, Celeste D. Rosencrance, Kyle P. Eagenvolume. 25 μL of 10x NEBuffer 2 (New England Biolabs) and 4 μL of 25 U/mL MboI (New England Biolabs #R0147) were added to the sample and then gently inverted to mix. The sample was incubated overnight at 37°

(5) Single-cell multiome and enhancer connectome of human retinal pigment epithelium and choroid nominate causal variants in macular degenerationCell ReportsJanuary 27, 2026Sean K. Wang, Jiaying Li, Surag Nair, Reshma Kosaraju, Yun Chen, Yuanyuan Zhang, Anshul Kundaje, Yuwen Liu, Ningli Wang, Howard Y. Changsulfate,Invitrogen,Cat# 15553027 Triton X-100,Sigma-Aldrich,Cat# 93443 MboI,New England BioLabs,Cat# R0147 Biotin-14-dATP,Jena Bioscience,Cat# NU-835-BIO14 Klenow fragment,New England BioLabs,Cat# M0210 T4 DNA ligase

(6) Distal enhancers regulate mammalian early embryonic lineage differentiation through long-range interactionsNucleic Acids ResearchJanuary 21, 2026Qianshu Zhu, Shimeng Guo, Juan Xie, Jiadan Qu, Xiaoyu Wan, Sicong Liang, Xiaoyu Liu, Ling Zhu, Jie Wang, Sheng Yang, et al.incubated at 37°C for 30 min, and 5 μl of 10× NEBuffer 2 and 50 U of the MboI restriction enzyme (NEB, R0147) were added to digest the chromatin overnight or for at least 2 h at 37°C with rotation. The sample

(7) Predictive prioritization of enhancers associated with pancreatic disease riskCell GenomJanuary 14, 2026Wang L, Baek S, Prasad G, Wildenthal J, Guo K, Sturgill D, Truongvo T, Char E, Pegoraro G, McKinnon K, et al.at 37°C for 15 min, rotating end-to-end. To digest the chromatin in situ, 100U MboI (Sigma-Aldrich R0147) and 25 μL 10xNEB buffer 2 (NEB B7002 RT) were added to the reaction and incubated at 37°C for 2 h with

(8) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.cocktail (MCE, HY-K0010). Nuclei were extracted and digested with the Mbo I restriction enzyme (NEB, R0147) for 2 hours. Following digestion, the nuclei were resuspended in NEB buffer and treated with DNA polymerase I, Large (Klenow) fragment

(9) Lineage-determining transcription factors constrain cohesin to drive multi-enhancer oncogene regulationNature Cell BiologyJanuary 1, 2026Yeqiao Zhou, Atishay Jay, Noah Burget, Tobias Friedrich, Sora Yoon, Jessica Alsing, Guy Nir, Rudolf Grosschedl, Golnaz Vahedi, Robert B. Faryabi(Invitrogen, cat. no. 15527–013). Chromatin was digested using MboI restriction enzyme (NEB, cat. no. R0147), followed by biotin incorporation with Biotin-14-dATP (Jena Bioscience, cat. no. NU-835-BIO14-S). DNA

(10) 3D enhancer architecture coordinated by CTCF determines immune-related gene expression patterns via RNA polymerase II pause-release in CD4+ T cellsNucleic Acids ResDecember 11, 2025Eun-Chong Lee, Kyungwoo Kim, Sugyung Kim, Mikyoung Kim, Hyoung-Pyo Kim(Duchefa Biochemie, G0709). Chromatin was digested using the MboI restriction enzyme (New England Biolabs, R0147), followed by biotin incorporation using Biotin-14-dATP (Jena Bioscience, NU-835-BIO14-S). After de-cross-linking,

(11) PKL mediates H3K4me2 modification and spatial gene congregation in chromatin regulationNucleic Acids ResDecember 8, 2025Mengfei Song, Yubo Wang, Tao Ma, William Terzaghi, Kai He, Huijie Wang, Bo Xu, Yanjun Jing, Rongcheng Lin, Xing Wang Deng, et al.Endogenous nuclease was inactivated with 0.3% (w/v) SDS, then chromatin was digested with 100 U of MboI (R0147L, NEB), marked with biotin-14-dCTP (N122T, GeneCodex), and then ligated by 50 U of T4 DNA ligase (EL0012,

(12) The 3D Genome Structure of a Blcap-Linked Silencer Loop Regulates Terminal Differentiation During Lens Fiber Cell DenucleationInvest Ophthalmol Vis SciDecember 8, 2025Shurui Ke, Zhong Liu, Jingyu Ma, Yingfeng Zheng, Lixia Luo(no. S19770; Acmec Biochemical, Shanghai, China). Chromatin was then digested with 100 U MboI (no. R0147S; New England Biolabs, Ipswich, MA, USA) and labeled with biotin-14-dCTP (no. 19518018; Invitrogen, Carlsbad,

(13) Mice carrying the homologous human shelterin POT1-L259S mutation linked to pulmonary fibrosis show a telomerase deficiency-like phenotype with telomere shortening with increasing mouse generationsGenes & developmentDecember 1, 2025Sánchez-Vázquez R, Burgaz García-Oteyza S, Serrano R, Flores JM, Martínez P, Blasco MA.× 106 cells were isolated and embedded in agarose plugs and digested with MboI (New England Biolabs R0147). DNA was separated by gel electrophoresis in 0.5× TBE maintained at 14°C using a CHEF-DR-II pulsed-field

(14) JG2: an updated version of the Japanese population-specific reference genomeHum Genome VarOctober 1, 2025Sirawit Sriwichaiin, Satoshi Makino, Takamitsu Funayama, Akihito Otsuki, Junko Kawashima, Yasunobu Okamura, Shu Tadaka, Fumiki Katsuoka, Kazuki Kumada, Shuichi Tsutsumi, et al.Tris–HCl pH 8.0, 10 mM NaCl and 0.2% Igepal CA-630), and the chromatin was digested by either MboI (NEB, R0147) or HindIII-HF (NEB). Both ends of the digested chromatin were filled in and labeled with biotin-14-dATP

(15) Small-scale in situ Hi-C protocol for early embryos to resolve the three-dimensional genome structureSTAR ProtocolsSeptember 19, 2025Linjie Song, Jingcheng Zhang, Linqian Pan, Shengjie Sun, Binqiang Shi, Hexu Zhang, Hengkuan Li, Zixiao Liu, Linmi Li, Yong Zhang, et al.inhibitor,Roche,Cat#4693132001 NEBuffer 2,NEB,Cat#B7002S SDS,BioFroxx,Cat#3250GR500 Triton X-100,Sigma-Aldrich,Cat#T8787 MboI,NEB,Cat#R0147S dCTP,Invitrogen,Cat#10217016 dGTP,Invitrogen,Cat#10218014 dTTP,Invitrogen,Cat#10219012 Nuclease-free

(16) Excessive DNA Double‐Strand Breaks–Associated 3D Genome Reorganization Contributes to Neural Tube Defects with Folate DeficiencyAdvanced ScienceSeptember 18, 2025Ting Zhang, Lin Lin, Jianting Li, Caihua Li, Shengjun Liang, Xuemei Bai, Fang Wang, Yihua Bao, Dan Guo, Xiaochen Bo, et al.mixture was incubated at 37 °C for 15 min. Next, 12 µL of 10×NEBuffer 2 and 400 units of MboI enzyme (R0147; NEB) were introduced for overnight digestion at 37 °C on a rocking platform. On the subsequent day,

(17) Aurora A regulates the material property of spindle poles to orchestrate nuclear organization at mitotic exitThe EMBO JournalSeptember 12, 2025Ashwathi Rajeevan, Vignesh Olakkal, Madhumitha Balakrishnan, Dwaipayan Chakrabarty, François Charon, Daan Noordermeer, Sachin Kotak101000046 Lipofectamine 2000,Life Technologies,Cat# 11668019 MboI restriction enzyme,New England Biolabs,Cat# R0147 Methanol,Merck,Cat# 34860 MK5108 (IC50-0.064 nM),TargetMol,Cat# T6068 MLN8237/Alisertib (IC50-1.2

(18) Human cytomegalovirus infection coopts chromatin organization to diminish TEAD1 transcription factor activityeLifeSeptember 10, 2025Sayeed K, Parameswaran S, Beucler MJ, Edsall LE, VonHandorf A, Crowther A, Donmez OA, Hass MR, Richards S, Forney CR, et al.0.2% NP-40, 1x Roche protease inhibitors) for 30 min at 4°C. Following cell lysis, 375 U of MboI (NEB, R0147) was used to digest, in situ, the crosslinked chromatin for 2 hr at 37°C. After filling-in the DNA ends

(19) A 3D genome compendium of breast cancer progressioniScienceAugust 5, 2025Teun van den Brand, Maria Donaldson Collier, Koen D. Flach, Sebastian Gregoricchio, Isabel Mayayo-Peralta, Zhanna Dauyey, Karianne Schuurman, Hans Teunissen, Wilbert Zwart, Elzo de Witpeptides, and recombinant proteins" ,, DAPI,Sigma-Aldrich,Cat# D9542 MboI,New England Biolabs,Cat# R0147L Biotin-14-dATP,Life Technologies,Cat# 19524-016 ,, Critical commercial assays,Critical commercial

(20) SUMO operates from a unique long tandem repeat to keep innate immunity in checkNucleic Acids ResearchJuly 31, 2025Amandine Goffeney, Ivo A Hendriks, Victoria Morel, Yann Loe-Mie, François Charon, Michael L Nielsen, Jack-Christophe Cossec, Daan Noordermeer, Jacob-Sebastian Seeler, Anne Dejeansnap freezing at −80°C. 4C libraries were generated as published [45] using MboI (New England Biolabs, R0147) as primary restriction enzyme and NlaIII (New England Biolabs, R0125) as secondary enzyme. 4C-seq sequencing

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MboI

MboI from New England Biolabs

Sequence

New England Biolabs

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MboI from New England Biolabs

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