PolyATtract mRNA Isolation System IV from Promega

Product Specs
ItemPolyATtract mRNA Isolation System IV
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ5310
Time45 min.
Quantity15 isolations
SourceReaction
Nucleic Acid TypemRNA

Specifications/Features

Starting Amount:
100–1,000µg mRNA / isolation

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) HPD is an m 6 A Methyltransferase that Protects Colorectal Cancer Cells from Ferroptotic Cell Death by m 6 A Methylating SLC7A11/GPX4Advanced ScienceNovember 29, 2025Jiyan Wang, Xintong Dai, Huanle Liu, Saiwei Hua, Hongkai Chang, Huanran Sun, Mingming Sun, Huifang Zhao, Kemin Ni, Fei Xie, et al.of total RNA or mRNA. The mRNA was enriched using the PolyATract mRNA isolation System IV (Promega, Z5310) following the manufacturer's instructions. In brief, total RNA or mRNA was mixed with 1 × SSC (saline

(2) Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidenceRNAApril 1, 2025Stanislav Stejskal, Veronika Rajecka, Helena Covelo-Molares, Ketty Sinigaglia, Kvetoslava Brozinova, Linda Kasiarova, Michaela Dohnalkova, Paul Eduardo Reyes-Gutierrez, Hana Cahova, Liam Peter Keegan, et al.the first step of poly(A) mRNA enrichment with the PolyATtract mRNA Isolation Systems kit (Promega Z5310) and purified according to the manufacturer's protocol. Eluted poly(A)+ mRNA was precipitated with 1

(3) YTHDC1 cooperates with the THO complex to prevent RNA-damage-induced DNA breaksMolecular CellMarch 20, 2025Ning Tsao (曹甯), Patrick M. Lombardi, Ajin Park, Jennifer Olabode, Rebecca Rodell, Hua Sun, Shilpa Padmanaban, Joshua R. Brickner, Miaw-Sheue Tsai (蔡妙雪), Elizabeth A. Pollina, et al.Kit,Qiagen,217004 RNA Clean & Concentrator-5 kit,Zymo Research,R1013 PolyATract mRNA isolation system IV,Promega,Z5310 SeqPlex RNA Amplification Kit,Millipore Sigma,SEQR Comet Assay,Trevigen,4250–050-K DNeasy Blood &

(4) The Flip Side of the Coin: METTL3 Serves as a Novel Cellular Senescence Accelerator via Negative Regulation of ITGA9aging and diseaseMarch 18, 2025Yuting Li, Linying Huang, Miaochun Fang, Liwen Ye, Haiqing Yang, Weijia Wu, Yuan Yuan, Kun Cao, Hui-Ling Zheng, Xuerong Sun, et al.was subsequently isolated from the total RNA using the PolyATtractR mRNA Isolation System IV (Promega Z5310, USA) according to the manufacturer’s instruction. The mRNAs were denatured by heating at 65°C for 10

(5) CRISPR screening reveals ZNF217 as a vulnerability in high-risk B-cell acute lymphoblastic leukemiaTheranosticsFebruary 18, 2025Xi Qin, Keren Zhou, Lei Dong, Lu Yang, Wei Li, Zhenhua Chen, Chao Shen, Li Han, Yangchan Li, Anthony K.N. Chan, et al.miRNeasy Mini Kit (217004, QIAGEN), and poly(A)+ mRNA was isolated using the PolyATtract mRNA Isolation Systems (Z5310, Promega), both according to their respective manufacturer's instructions. In the dot blot assays, total

(6) YTHDF2 promotes ATP synthesis and immune evasion in B cell malignanciesCellJanuary 23, 2025Zhenhua Chen, Chengwu Zeng, Lu Yang, Yuan Che, Meiling Chen, Lillian Sau, Bintao Wang, Keren Zhou, Yu Chen, Ying Qing, et al.Assays CellTiter-Glo® 2.0 Cell Viability Assay,Promega,Cat# G9241 PolyATtract® mRNA Isolation System IV,Promega,Cat# Z5310 Seahorse XF Cell Mito Stress Test Kit,Seahorse Bioscience,Cat# 103015-100 CD3 MicroBeads,Miltenyi Biotec

(7) Designing strong inducible synthetic promoters in yeastsNature CommunicationsDecember 19, 2024Masahiro Tominaga, Yoko Shima, Kenta Nozaki, Yoichiro Ito, Masataka Someda, Yuji Shoya, Noritaka Hashii, Chihiro Obata, Miho Matsumoto-Kitano, Kohei Suematsu, et al.cDNA was obtained via reverse transcription using the PolyATtract® mRNA Isolation Systems (Cat. No. Z5310, Promega) and the PrimeScript II 1st Strand cDNA Synthesis Kit (Cat. No. 6210 A, Takara Bio). Phage-based

(8) Stable structures or PABP1 loading protects cellular and viral RNAs against ISG20-mediated decayLife Science AllianceMay 1, 2024Camille Louvat, Séverine Deymier, Xuan-Nhi Nguyen, Emmanuel Labaronne, Kodie Noy, Marie Cariou, Antoine Corbin, Mathieu Mateo, Emiliano P Ricci, Francesca Fiorini, et al.Invitrogen). mRNAs were then isolated using PolyATtract mRNA Isolation Systems following the instruction (cat. Z5310; Promega). RNA was quantified by NanoDrop, and the number of mRNA molecules has been roughly estimated,

(9) Plasticity of gene expression in the nervous system by exposure to environmental odorants that inhibit HDACseLifeFebruary 27, 2024Sachiko Haga-Yamanaka, Rogelio Nunez-Flores, Christi A Scott, Sarah Perry, Stephanie Turner Chen, Crystal Pontrello, Meera G Nair, Anandasankar Ray,HDAC6 Fluorogenic Assay Kit,BPS Bioscience,50076, Commercial assay or kit,PolyATtract mRNA Isolation System,Promega,Z5310, Commercial assay or kit,Superscript III,Invitrogen,18080093, Commercial assay or kit,SYBR Green Master

(10) Cell-type-specific control of secondary cell wall formation by Musashi-type translational regulators in ArabidopsiseLifeSeptember 29, 2023Alicia Kairouani, Dominique Pontier, Claire Picart, Fabien Mounet, Yves Martinez, Lucie Le-Bot, Mathieu Fanuel, Philippe Hammann, Lucid Belmudes, Remy Merret, et al.RNA obtained by the TRI Reagent extraction, using the PolyATtract mRNA Isolation System III (Cat. No. Z5310; Promega) according to the manufacturer’s instructions. cDNAs were then synthetized according to the

(11) Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61 m6AEnvironmental Health PerspectivesSeptember 8, 2023Ya-Ping Song, Jin-Wei Lv, Zhi-Cheng Zhang, Qing-Hua Qian, Yi-Jun Fan, Dao-Zhen Chen, Heng Zhang, Fei-Xiang Xu, Cheng Zhang, Yichao Huang, et al.kit. Total RNA was extracted using TRI reagent and purified by PolyATtract mRNA isolation systems (Z5310, Promega). Each group contained five biologically independent replications. The level of m6A in 100

(12) TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewalCell stem cellAugust 3, 2023Li Y, Xue M, Deng X, Dong L, Nguyen LXT, Ren L, Han L, Li C, Xue J, Zhao Z, et al.RNA Immunoprecipitation and qPCR Poly(A)+ RNA was enriched from total RNA with polyATract mRNA isolation system IV kit (Z5310, Promega) following manufacturer’s instructions. m5C RNA immunoprecipitation (MeRIP) was performed as

(13) Transcriptome-wide Dynamics of m(6)A mRNA Methylation During Porcine SpermatogenesisGenomics, proteomics & bioinformaticsAugust 1, 2023Liu Z, Chen X, Zhang P, Li F, Zhang L, Li X, Huang T, Zheng Y, Yu T, Zhang T, et al.15596026, Invitrogen). mRNA was isolated using the PolyATtract mRNA Isolation Systems (Catalog No. Z5310, Promega, Madison, WI) following the manufacturer’s instructions. IP mixture was composed by 6 µg of

(14) QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolismCellJuly 20, 2023Zhao Z, Qing Y, Dong L, Han L, Wu D, Li Y, Li W, Xue J, Zhou K, Sun M, et al.modification.17 The polyA+ mRNAs were enriched from total RNAs with polyATract mRNA isolation system IV (Z5310, Promega) and the 5’ Cap was removed from polyA+ mRNAs with Tobacco Decapping Plus 2 (94, Enzymax).

(15) M6A modification promotes blood-brain barrier breakdown during cerebral ischemia/reperfusion injury through increasing matrix metalloproteinase 3 expressionHeliyonJune 1, 2023Liang E, Xiao S, Zhao C, Zhang Y, Fu G.cells using TRIZOL (Thermo). mRNA was isolated using the PolyATtract® mRNA Isolation Systems (Promega, Z5310) following the manufacturer's instructions. IP mixture was composed by 6 μg rabbit anti m6A antibody

(16) METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolismCell stem cellJanuary 5, 2023Han L, Dong L, Leung K, Zhao Z, Li Y, Gao L, Chen Z, Xue J, Qing Y, Li W, et al.NOMO-1 cells using TRIzol reagent. mRNA was further purified by PolyATtract® mRNA Isolation Systems (Z5310, Promega). 2ug mRNA was fragmented into 100~200 nt with RNA Fragmentation Buffer at 70°C for 10min.

(17) A multiplex platform for small RNA sequencing elucidates multifaceted tRNA stress response and translational regulationNature CommunicationsDecember 1, 2022Christopher P. Watkins, Wen Zhang, Adam C. Wylder, Christopher D. Katanski, Tao PanPolyA+ RNA was extracted from the input and polysome RNA using a polyA+ RNA extraction kit from Promega (Z5310) or NEB (E7490S), respectively. Poly(A)-selection was done with NEBNext Poly(A) mRNA Magnetic Isolation Module

(18) m(6)A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancerCancer gene therapyOctober 1, 2022Tsuchiya K, Yoshimura K, Iwashita Y, Inoue Y, Ohta T, Watanabe H, Yamada H, Kawase A, Tanahashi M, Ogawa H, et al.–mass spectrometry/mass spectrometry (LC–MS/MS) PolyA-enriched RNA was extracted using PolyATract mRNA isolation systems (#Z5310 Promega, Madison, WI, USA) according to the manufacturer’s instructions. PolyA-enriched RNA concentration

(19) METTL16 exerts an m(6)A-independent function to facilitate translation and tumorigenesisNature cell biologyFebruary 1, 2022Su R, Dong L, Li Y, Gao M, He PC, Liu W, Wei J, Zhao Z, Gao L, Han L, et al.kit (217004, Qiagen) according to the manufacturer’s protocol. PolyATract mRNA isolation system IV (Z5310, Promega) was applied to enrich the mRNA from total RNA. The RNA concentration was measured by a NanoDrop

(20) Interferon inducible pseudouridine modification in human mRNA by quantitative nanopore profilingGenome BiologyDecember 6, 2021Sihao Huang, Wen Zhang, Christopher D. Katanski, Devin Dersh, Qing Dai, Karen Lolans, Jonathan Yewdell, A. Murat Eren, Tao PanRNA from 50 μg total RNA of each sample was extracted by Promega PolyATtract® mRNA Isolation Systems Z5310. Each sample was eluted with 15 μL H2O. Prediction of Ψ in HeLa samples The raw data of two replicates

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