PolyATtract mRNA Isolation System III with Magnetic Stand from Promega

Product Specs
ItemPolyATtract mRNA Isolation System III with Magnetic Stand
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ5300
Time45 min.
Quantity15 isolations
SourceReaction
Nucleic Acid TypemRNA
FormatMagnetic

Specifications/Features

Starting Amount:
100–1,000µg mRNA / isolation

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemiaThe EMBO JournalFebruary 2, 2026Tian-Qi Chen, Yu-Meng Sun, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Heng-Jing Huang, Qi Pan, Jun-Yi Lian, Wei Huang, Ke Fang, et al.TRIzol reagent (Invitrogen, 15596018), and mRNA was purified using Dynabeads Oligo(dT)25 (Promega, Z5300). mRNA was fragmented by incubation at 94 °C for 4 min with fragment buffer (NEB, E6150S). Two hundred

(2) Scaling perturbations: beyond genome-scale CRISPR screensbioRxivJanuary 21, 2026Anran Tang, Rico C. Ardy, Rafaela E. Mendes, Thomas M. NormanMidi kit (Qiagen, 75144). mRNA was isolated using PolyATtract® mRNA Isolation Systems III (Promega, Z5300). Template-switching first strand cDNA was synthesized using Maxima H Minus Double-Stranded cDNA Synthesis Kit

(3) D-2-HG Inhibits IDH1mut Glioma Growth via FTO Inhibition and Resultant m6A HypermethylationCancer Res CommunMarch 22, 2024Pianka ST, Li T, Prins TJ, Eldred BSC, Kevan BM, Liang H, Zapanta Rinonos S, Kornblum HI, Nathanson DA, Pellegrini M, et al.TRIzol Reagent (Ambion, catalog no. 15596018). PolyATtract mRNA Isolation Systems Kit (Promega, catalog no. Z5300) was used to purify mRNA from total RNA samples. m6A RNA Dot Blot Total RNA and poly A+ purified mRNA

(4) Identification of Metabolism-Related Proteins as Biomarkers of Insulin Resistance and Potential Mechanisms of m6A ModificationNutrientsApril 11, 2023Yan-Ling Li, Long Li, Yu-Hong Liu, Li-Kun Hu, Yu-Xiang Yanmethylated RNA immunoprecipitation (MeRIP) assay according to the manufacturer’s instructions. Next, using an mRNA purification kit (Z5300; Promega, USA), a portion of the total RNA was converted into mRNA. The enriched RNA was immediately

(5) Tumor-suppressive MEG3 induces microRNA-493-5p expression to reduce arabinocytosine chemoresistance of acute myeloid leukemia cells by downregulating the METTL3/MYC axisJournal of Translational MedicineDecember 1, 2022Airong Wang, Yufei Chen, Luyao Shi, Mengya Li, Lingling Li, Shujuan Wang, Chong Wanghelp of the Trizol method followed by purification through PolyATtract mRNA Isolation Systems (No. A-Z5300, AD Technology, Beijing, China). Then, mRNAs were subjected to denaturation for 7 min under ultraviolet

(6) Mettl14-mediated m6A modification is essential for visual function and retinal photoreceptor survivalBMC biologyJune 13, 2022Yang Y, Shuai P, Li X, Sun K, Jiang X, Liu W, Le W, Jiang H, Liu Y, Zhu X.dot blot assays mRNA was purified from total RNA using PolyATtract® mRNA Isolation System III (Cat#Z5300, Promega, Madison, WI, USA) following the protocols supplied with the kit. The mRNAs were subjected

(7) Novel Variations in the KDM5C Gene Causing X-Linked Intellectual DisabilityNeurology. GeneticsFebruary 1, 2022Wu PM, Yu WH, Chiang CW, Wu CY, Chen JS, Tu YF.purified from at least 50 µg of total RNA using PolyA + Track mRNA Isolation System III from Promega (#Z5300), according to the manufacturer's instructions. First-strand cDNA was synthesized from total RNA using

(8) Coupling of spliceosome complexity to intron diversityCurrent BiologyNovember 1, 2021Jade Sales-Lee, Daniela S. Perry, Bradley A. Bowser, Jolene K. Diedrich, Beiduo Rao, Irene Beusch, John R. Yates, Scott W. Roy, Hiten D. MadhaniAssays,Critical Commercial Assays,Critical Commercial Assays PolyATtract® mRNA Isolation System,Promega,Z5300 NEBNext Ultra Directional RNA Library Prep Kit for Illumina,New England Biolabs,E7420S RNA 6000 Pico

(9) 细菌转录组分析样品制备方法BIO-PROTOCOLJanuary 1, 2021迎春武, 光飞郝, 东飞韩测序样品准备相关步骤知之甚少，基于此本文将对细菌转录组样本制备过程进行详细说明。关键词：细菌，转录组，样品制备，RNA-Seq 材料与试剂 1. 50x TAE 缓冲液 (Aladdi, catalog number: T197242) 2. DNA Ladder (Promega, catalog number: Z5300) Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC. 1 www.bio-protocol.org/e2003650 DOI:10.21769/BioProtoc.2003650 3. Agarose

(10) Ethephon treatment reduced Mondo grass (Ophiopogon japonicus) gas exchange rate and gene expression of RbcsEuropean Journal of Horticultural ScienceApril 29, 2019Jun Liu, Institute of Turfgrass Science, Beijing Forestry University, Beijing, China, Lixin Xu, Yingbo Wang, Liebao Han, College of Agro-grassland Sciences, Nanjing Agricultural University, Nanjing, China, Institute of Turfgrass Science, Beijing Forestry University, Beijing, China, Institute of Turfgrass Science, Beijing Forestry University, Beijing, China, Institute of Turfgrass Science, Beijing Forestry University, Beijing, China18S rRNA through agarose gel electrophoresis. Poly (A+) RNA was isolated by PolyA Tract Systems II (Z5300) (Promega, Beijing) under standard conditions given in the manual. cDNA library construction A full-length

(11) Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m⁶A mRNA MethylationCell reportsNovember 13, 2018Zhong X, Yu J, Frazier K, Weng X, Li Y, Cham CM, Dolan K, Zhu X, Hubert N, Tao Y, et al.Industries,933–00404 HDL assay kit,Wako Pure Chemical Industries,993–72691 PolyATtract® mRNA Isolation System,Promega,Z5300 Ribominus eukaryote kit v2,Life technologies,A15020 anti-flag M2 magnetic beads,Sigma,M8823 ,, Deposited

(12) Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixturesBMC GenomicsDecember 1, 2017Rafael Luis Kessler, Daniela Parada Pavoni, Marco Aurelio Krieger, Christian Macagnan Probstpurified from at least 50 μg of total RNA using PolyA + Track® mRNA Isolation System III from Promega® (#Z5300), according to the manufacturer’s instructions. RNA amplification All mRNA amplification reactions

(13) Comparative study of Arabidopsis PBS1 and a wheat PBS1 homolog helps understand the mechanism of PBS1 functioning in innate immunityScientific ReportsDecember 1, 2017Jianhang Sun, Guozhong Huang, Fenggui Fan, Shuangfeng Wang, Yingying Zhang, Yufang Han, Yanmin Zou, Dongping Luobtained by 5′ RACE and 3′ RACE PCR. PolyATtract® mRNA Isolation Systems III with Magnetic Stand (product Z5300, Promega Corporation) was used for mRNA enrichment, then the 5′ and 3′ RACE PCR were conducted using

(14) Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cellsBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicasSeptember 12, 2017Guo N, Zhang N, Yan L, Cao X, Lv F, Wang J, Wang Y, Cong H.manufacturer’s instructions and the mRNA was isolated using PolyA Ttract® mRNA Isolation System III (Z5300) Kit (Promega, USA) as described in the user manual. The quality and quantity of total RNA and mRNA

(15) Konstruksi pustaka cDNA dari daun klon karet AVROS 2037 yang diinfeksi patogen Corynespora cassiicola Construction of a cDNA library from leaf of AVROS 2037 rubber clone infected by Corynespora cassiicola pathogenE-Journal Menara PerkebunanMarch 12, 2016. NURHAIMI-HARIS, Hajrial ASWIDINNOOR, Antonius SUWANTO, Maggy T. SUHARTONO, Nurita TORUAN-MATHIUS, Agus PURWANTARA1989). Untuk mengisolasi mRNA dari RNA digunakan Poly ATract mRNA Isolation System III (Promega Cat. Z5300), yang pelaksanaannya dilakukan sesuai dengan prosedur yang direkomendasikan dalam petunjuk teknisnya.

(16) Novel candidate genes AuxRP and Hsp90 influence the chip color of potato tubersMolecular BreedingDecember 1, 2015Dorota Sołtys-Kalina, Katarzyna Szajko, Izabela Sierocka, Jadwiga Śliwka, Danuta Strzelczyk-Żyta, Iwona Wasilewicz-Flis, Henryka Jakuczun, Zofia Szweykowska-Kulinska, Waldemar Marczewskiof total RNA from bulk samples LII and DII using the PolyATract mRNA Isolation System (Promega, No. Z5300) according to manufacturer’s protocol. Double-stranded cDNA (ds-cDNA) was synthesized using the cDNA Synthesis System

(17) A Subset of Histone H2B Genes Produces Polyadenylated mRNAs under a Variety of Cellular ConditionsPLoS ONEMay 22, 2013Vijayalakshmi Kari, Oleksandra Karpiuk, Bettina Tieg, Malte Kriegs, Ekkehard Dikomey, Heike Krebber, Yvonne Begus-Nahrmann, Steven A. JohnsenmRNA was isolated from 100 µg of total RNA using the PolyATtract® mRNA Isolation System III (Cat. No. Z5300, Promega, Wisconsin) according to the manufacturer’s instructions. Total or polyA+ RNA was reverse transcribed

(18) Identification of a novel circularized transcript of the AML1 geneBMB ReportsMarch 31, 2013Ai-Ning Xu, Xiu-Hua Chen, Yan-Hong Tan, Xi-Ling Qi, Zhi-Fang Xu, Lin-Lin Zhang, Fang-Gang Ren, Si-Cheng Bian, Yi Chen, Hong-Wei Wangmanufacturer. The mRNA was isolated from total RNA using PolyATtractⓇ mRNA Isolation Systems III (Promega, Cat#Z5300), and the poly(A)-RNA was recycled from the supernatant by ethanol precipitation. Purity and concentration

(19) Genome organization and characteristics of soybean microRNAsBMC GenomicsDecember 1, 2012Marie Turner, Oliver Yu, Senthil Subramanianassay. First, polyA mRNA was isolated from total RNA using PolyAtract mRNA isolation systems (Promega Z5300). 5’RLM-RACE was performed on the resulting mRNA preparation using Generacer Kit (Invitrogen, Carlsbad,

(20) Identification of genes involved in bamboo fiber developmentGeneJanuary 6, 2011Vineeta Rai, Jayadri Sekhar Ghosh, Amita Pal, Nrisingha Dey

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