KpnI-HF from New England Biolabs

Product Specs
ItemKpnI-HF
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR3142S
Quantity4000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Mutual potentiation of IPA1 and OsNPR1 enhances rice immunityPlant CellMay 20, 2026Zhen Wang, Mingming Liu, Rujuan Yan, Xiaohan Zhang, Xiaoguang Song, Guosheng Xiong, Jiayang Li, Zuhua He, Dong-Lei Yangcloned into the pCAMBIA1305-3xFLAG vector, which had been digested with EcoRI (R3101S, NEB) and KpnI (R3142S, NEB) enzymes, using T4 DNA ligase (M0202V, NEB). For OsNPR1-GFP, the coding region of OsNPR1 without

(2) A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signalingSci AdvMay 15, 2026Miroslav Micka, Jitender Kumar, Petra Paclíková, Zuzana Hayek, Kateřina Hanáková, Cherine Bechara, Hana Plešingerová, Ondrej Šedo, Sara Bologna, Elise Del Nero, et al.via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into

(3) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapatfor each cloning strategy. For the full-length and Δ constructs (Δ1 and Δ2) in pUAST, KpnI-HF (NEB, #R3142S) and NotI (NEB, #R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S)

(4) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldusing FseI (New England Biolabs, Catalog no: R0588L) and KpnI-HF (New England Biolabs, Catalog no: R3142S). This new plasmid is designated as B-SpCas9-HF1-2A-PuroR. Single guide RNA cloning Single guideRNAs

(5) In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and TestingbioRxivMarch 25, 2026Enrique O. Ruiz, Kayla Neyra, Diana M. Lopez, Ruo-Wen Chen, Deepta Paramasamy, Quincy Bizjak, Patrick D. Halley, Yin Wei, Marcos Sotomayor, Michael G. Poirier, et al.was acquired from PerkinElmer (cat. #CLS157950). The restriction enzymes PstI-HF (R3140S), KpnI-HF (R3142S), and PvuI-HF (R3150S) were purchased from New England Biolabs. Genetic Analysis Grade Agarose (CAS

(6) Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidneyCell ReportsMarch 24, 2026Kohei Omachi, Meei-Hua Lin, Pongpratch Puapatanakul, Joshua F. Begin, Karen K. McKee, Hironobu Fujiwara, Peter D. Yurchenco, Jeffrey H. Minerpreviously. Tg: Lama2-gRNA mice were produced in this study. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System

(7) Hemocyte-secreted Papilin bearing mucin-type O-glycans regulates peripodial stalk formation via epidermal JAK/STAT signaling in DrosophilaiScienceMarch 20, 2026Takashi J. Fuwa, Kazuyoshi Itoh, Tomomi Ichimiya, Yoshihiro Akimoto, Shoko Nishihara,Thermo Fisher Scientific,Cat# S21374 EcoRI-HF,New England Biolabs,Cat# R3101S KpnI-HF,New England Biolabs,Cat# R3142S XhoI,New England Biolabs,Cat# R0146S Cellfectin™ II Reagent,Thermo Fisher Scientific,Cat# 10362100 Sf-900TM

(8) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko Iwasakifrog, sinicus bat allele 3358) by Twist Bioscience. ACE2 gene fragments were cloned into KpnI (NEB R3142S) and MluI (R3198S) double-digested pCMV6-MYC-FLAG plasmid backbone using the InFusion cloning kit (Takara

(9) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.The plasmid has puro-2a-luciferase-2a-GFP-NLS, we replaced GFP-NLS with mCherry-NLS using KpnI (NEB # R3142S) and MluI (NEB # R3198S) restriction enzymes and their respective restriction sites resulting in a mCherry-Luc

(10) Universal Baseline for in vitro Selection of Genetically Encoded LibrariesbioRxivFebruary 18, 2026Kejia Yan, Guilherme M. Lima, Tara Bahadur, Vincent Albert, Zoe O’Gara, Gary Bao, Christin Kossmann, William Kirby, Fernando B. Mejia, Matthew L. Michnik, et al.(43). In short, to produce SX3CX9C library, the vector SB4 QFT*LHQ was digested with KpnI HF (NEB Cat# R3142S) and EagI HF (NEB Cat# R3505S). A primer/template pair consisting of annealed primer 5′-AT GGC GCC CGG

(11) A prophage-expressed type IV pilus component provides anti-phage defenseCell ReportsJanuary 27, 2026Kristina M. Sztanko, Taylor J. Ellison, Jill Greenlaw, Tatiana Lenskaia, P. Nathael Javorčík, Alexa D. Fitzpatrick, Karen L. Maxwell, Courtney K. Ellison, Alan R. Davidson,New England Biolabs,Cat# R3101S HindIII-HF,New England Biolabs,Cat# R3104S KpnI-HF,New England Biolabs,Cat# R3142S XbaI-HF,New England Biolabs,Cat# R0145S BsaI-HF v2,New England Biolabs,Cat# R3733S T4 DNA Ligase,New England Biolabs

(12) Construction and Biological Characterization of ORF133-Deletion Mutant of Lumpy Skin Disease VirusVirusesJanuary 24, 2026Qunhua Ke, Miaomiao Li, Yao Peng, Xiangwei Wang, Yuefeng Sun, Xiangping Yin, Yanming WeiBuffer,P0013C HindIII-HF®,R3104S,Neb,Ipswich/MA/USA Neb,Ipswich/MA/USA,EcoRI-HF®,R3101T Neb,Ipswich/MA/USA,KpnI-HF®,R3142S Neb,Ipswich/MA/USA,XbaI,R0145S Neb,Ipswich/MA/USA,T4 DNA Ligase,M0202S WestemBright ECL,K-12045-D50,Advansta,Menlo

(13) Integrative transcriptome-wide association analyses reveal PRKCG-linked GABAergic dysfunction in Fragile X-associated tremor/ataxia syndromeNature CommunicationsJanuary 8, 2026Yulin Jin, Yiqu Cao, Wenjing Ma, Ronghua Li, Yujing Li, Yunhee Kang, Jing Huang, Michael P. Epstein, Xiangxue Guo, Junghwa Lim, et al.PRKCG insert and the pUAST backbone were digested with Not1 and Kpn1 (New England Biolabs, R3189S and R3142S) at 37 °C for 30 min and ligated using T4 DNA ligase (New England Biolabs, M0202) at 16 °C overnight,

(14) Splice-switching ASOs targeting the AURKA 5′ UTR collapse an SRSF1-AURKA-MYC oncogenic circuit in pancreatic cancerMolecular CellJanuary 8, 2026Alexander J. Kral, Lu Jia, GeunYoung Sim, Ledong Wan, Yuma Ishigami, Adrian R. KrainerR3104S NcoI-HF,NEB,Cat# R3193S EcoR1-HF,NEB,Cat# R3101S AgeI-HF,NEB,Cat# R3552S DdeI,NEB,Cat# R0175S KpnI-HF,NEB,Cat# R3142S NcoI-HF,NEB,Cat# R3193S Polybrene,Sigma,Cat# TR-1003-G NEBuffer 2,NEB,Cat# B7002S T4 DNA Ligase,NEB,Cat#

(15) Fertilization triggers early proteomic symmetry breaking in mammalian embryosCellDecember 24, 2025Lisa K. Iwamoto-Stohl, Aleksandra A. Petelski, Baiyi Quan, Maciej Meglicki, Audrey Fu, Shoma Nakagawa, Breanna McMahon, Ting-Yu Wang, Saad Khan, Harrison Specht, et al.ligation (New England Biolabs, M0202S). pRN3P-mCherry was linearized using KpnI (New England Biolabs, R3142S). In vitro transcription was carried out using the mMessage mMachine T3 kit (Thermo Fisher, AM1348)

(16) Clinical relevance of zebrafish for gene variants testing. Proof-of-principle with SMN1/SMAEMBO Molecular MedicineDecember 15, 2025Brett W Stringer, Yougang Zhang, Afsaneh Taghipour-Sheshdeh, Shuxiang Goh, Heike Kölbel, Michelle A Farrar, Brunhilde Wirth, Jean Giacomotto#1791100 GeneJET Plasmid Miniprep Kit,ThermoFisher Scientific,Cat #K0503 KpnI-HF,New England BioLabs,Cat #R3142S PureLink™ Quick Gel Extraction and PCR Purification Combo Kit,ThermoFisher Scientific,Cat #K220001 mMESSAGE mMACHINE™ T3 Transcription Kit

(17) The RNA-binding protein PRRC2B preserves 5' TOP mRNA during starvation to maintain ribosome biogenesis during nutrient recoveryNucleic Acids ResDecember 11, 2025Nadav Goldberg, Doron Bril, Miriam Eisenstein, Tsviya Olender, Alon Savidor, Shani Bialik, Shmuel Pietrokovski, Adi Kimchipolymerase SuperScript III (Invitrogen, cat# 18 080 044); Restriction enzymes KpnI, EcorV, DpnI (NEB, cat# R3142S, R3195S, R0176S). Instruments used included: for fluorescence microscopy, a LSM900 confocal microscope

(18) Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometrySTAR ProtocolsDecember 1, 2025Matias Cardenas, Sasha Compton, Ashkon Hunt, Pradeep Chopra, Daniel R. Perez, Geert-Jan Boons, Daniela S. Rajaoassays ,, BamHI,New England Biolabs,R0136S EcoRI,New England Biolabs,R0101S KpnI- HF,New England Biolabs,R3142S XhoI,New England Biolabs,R0146S QIAprep spin miniprep kit,QIAGEN,27104 Ni-NTA agarose,Thermo Fisher Scientific

(19) The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damageThe EMBO JournalNovember 20, 2025Alexandra Boegli, Elliott M Bernard, Louise Lacante, Gaël Majeux, Ella Hartenian, Vanessa Mack, Petr Brozresistance cassette was replaced by a Hygromycin cassette using BamHI-KpnI (New England Biolabs #R3136S and #R3142S) restriction digest and In-Fusion cloning. Human NLRP6 expression plasmid was obtained from Sino Biological

(20) Defining the functional role and potential as an immunotherapeutic target of ALCAM in neuroblastomaMolecular Cancer TherapeuticsNovember 7, 2025Jarrett Lindsay, Jonathan Gaither, David Groff, Minu Samanta, Rebecca Kaufman, Liron Grossmann, Nicholas Hartnett, Nathan Kendsersky, Emily A. Mycek, Sharon J. Diskin, et al.#28704), then restriction digested with SacI-HF (NEB, #R31156S) and XhoI (NEB, #R0146S), or KpnI-HF (NEB, #R3142S) and XhoI. After heat inactivation, samples were separated on a 1% agarose gel run at 100V for approximately

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KpnI-HF

KpnI-HF from New England Biolabs

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New England Biolabs

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KpnI-HF from New England Biolabs

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