KpnI-HF from New England Biolabs

Product Specs
ItemKpnI-HF
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR3142L
Quantity20000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.,New England Biolabs,Cat#R0176L BamHI-HF,New England Biolabs,Cat#R3136L KpnI-HF,New England Biolabs,Cat#R3142L Shrimp Phosphatase,New England Biolabs,Cat#M0371S DNase I (RNase-free),New England Biolabs,Cat#M0303L on-column

(2) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.,ThermoFisher Scientific,Cat#FD1744 Eco47III,ThermoFisher Scientific,Cat#FD0324 Eco32I,ThermoFisher Scientific,Cat#FD0304 KpnI,New England Biolabs,Cat#R3142L NdeI,New England Biolabs,Cat#R0111L BamHI,New England Biolabs,Cat#R0136L NcoI,New England Biolabs,Cat#R0193L FastAP,ThermoFisher Scientific

(3) CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infectionInfection and ImmunityDecember 16, 2025Xavier Tijerina, C.A. Jabeena, Robert Faris, Zhen Xu, Parker Smith, Nicholas J. Schnicker, Mary M. WeberC-terminal HA-tag or FLAG-tag was added. The resulting PCR fragments were digested with KpnI-HF (NEB Cat# R3142L) and SalI-HF (NEB Cat# R3138L) and cloned into pBomb4. To truncate or specifically delete the CC regions,

(4) Protocol to screen chemicals to enhance homology-directed repair in CRISPR-Cas9 gene editingSTAR ProtocolsSeptember 19, 2025Min Ju Jo, Hye Jin NamPoly-D-lysine hydrobromide,Sigma-Aldrich,Cat#P64607 Dimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat#D8418 KpnI-HF,New England Biolabs,Cat#R3142L AflIII,New England Biolabs,Cat#R0541S AatII,New England Biolabs,Cat#R0117S BbsI,New England Biolabs,Cat#R0539S T4 DNA ligase

(5) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai LiT4 DNA ligase,New England Biolabs,M0202S Aar I,Thermo Fisher Scientific,ER1581 Kpn I,New England Biolabs,R3142L Bgl II,New England Biolabs,R0144L EcoR I,New England Biolabs,R3101S Sac I,New England Biolabs,R3156L Pst

(6) Disturbing Cholesterol/Sphingolipid Metabolism by Squalene Epoxidase Arises Crizotinib HepatotoxicityAdvanced ScienceJanuary 21, 2025Hao Yan, Xiangliang Huang, Yourong Zhou, Yuan Mu, Shaoyin Zhang, Yashi Cao, Wentong Wu, Zhifei Xu, Xueqin Chen, Xiaochen Zhang, et al.restriction digestion of the pcDNA3.0 vector plasmids with KpnI and BamHI, followed by ligations. KpnI‐HF (R3142L), BamHI‐HF (R3136L) and T4 DNA ligase (M0202S) were purchased from New England Biolabs (Ipswich, USA).

(7) ISWI catalyzes nucleosome sliding in condensed nucleosome arraysNature Structural & Molecular BiologySeptember 1, 2024Petra Vizjak, Dieter Kamp, Nicola Hepp, Alessandro Scacchetti, Mariano Gonzalez Gonzalez Pisfil, Joseph Bartho, Mario Halic, Peter B. Becker, Michaela Smolle, Johannes Stigler, et al.presence of condensates was confirmed by microscopy. To each tube, 2.5 μL of KpnI (New England Biolabs; R3142L) was added and incubated at 26 °C for 30 min. Cleavage was detected as above for the restriction-based

(8) Neutralizing immunity against coronaviruses in Tanzanian health care workersSci RepMarch 6, 2024Godfrey Barabona, Isaac Ngare, Doreen Kamori, Lilian Nkinda, Yusuke Kosugi, Ambele Mawazo, Rayi Ekwabi, Gloria Kinasa, Harrison Chuwa, The Genotype to Phenotype Japan (G2P-Japan) Consortium, et al.gene synthesis service (Fasmac). These plasmids were fragmented by restriction enzymes (KpnI, NEB, Cat# R3142L; NotI, NEB, Cat# R3189L) and cloned into the KpnI-NotI site of backbone pCAGGS vector 19 using T4 DNA Ligase

(9) Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarityMolecular Biology of the CellFebruary 1, 2024Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammerbackbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L). To create the rescued cell lines, KO-1 cells were nucleofected using an Amaxa nucleofection apparatus

(10) High-throughput functional analysis of regulatory variants using a massively parallel reporter assaySTAR ProtocolsDecember 1, 2023Kate Delfosse, Chiara Gerhardinger, John L. Rinn, Philipp G. MaassT4 DNA ligase,NEB,Cat#M0202M T4 DNA ligase reaction buffer,NEB,Cat#B0202S XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L dNTP solution mix - 40 μmol of each,NEB,Cat#N0447L Formamide (deionized),Invitrogen,Cat#AM9342 Agencourt

(11) Galectin-3 Mediates Vascular Dysfunction in Obesity by Regulating NADPH Oxidase 1Arteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2023Caleb A. Padgett, Róbert K. Bátori, Andrew C. Speese, Cody L. Rosewater, Weston B. Bush, Cassandra C. Derella, Stephen B. Haigh, Hunter G. Sellers, Zachary L. Corley, Madison A. West, et al.AgRP (Origene CAT#: RC217144) was cloned into CAG-NLS-GFP (Addgene#: 104061) between KpnI (NEB CAT#: R3142L) and EcoRI (NEB CAT# R3101L) restriction enzyme sites. AAVs were generated following the protocol detailed

(12) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.Phosphatase, Calf Intest",NEB,Cat#M0290L T4 DNA Ligase,NEB,Cat#M0202M XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L Q5- High Fidelity DNA polymerase,NEB,Cat#M0491L Formamide (Deionized),Invitrogen,Cat#AM9342 Agencourt

(13) Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphaeCurrent BiologyJuly 10, 2023Ingrid Richter, Philipp Wein, Zerrin Uzum, Claire E. Stanley, Jana Krabbe, Evelyn M. Molloy, Nadine Moebius, Iuliia Ferling, Falk Hillmann, Christian Hertweck,New England Biolabs,CAT#M0530L Apramycin,Sigma,CAT#A2024 SpeI,New England Biolabs,CAT#R3133L KpnI,New England Biolabs,CAT#R3142L OneTAQ® Quick-Load® 2x Master Mix,New England Biolabs,CAT#M0486L Ethidium bromide,New England Biolabs,CAT#E1510 NdeI,New England Biolabs

(14) Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolaritybioRxivJune 20, 2023Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammerbackbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L). To create the rescued cell lines, HeLa Myo10 KO1 cells were nucleofected using an Amaxa nucleofection

(15) SLC2A9 rs16890979 reduces uric acid absorption by kidney organoidsFrontiers in cell and developmental biologyJanuary 1, 2023Wu S, Li C, Li Y, Liu J, Rong C, Pei H, Li X, Zeng X, Mao W.the upstream-pBluescriptKS and the downstream 202-bp fragment using XhoI (NEB, R0146L) and KpnI (NEB, R3142L) and ligated the fragments with T4 ligase (Promega, M1794). After that, we amplified the same fragments

(16) Formation of synthetic RNA protein granules using engineered phage-coat-protein -RNA complexesNat CommunNovember 10, 2022Naor Granik, Noa Katz, Or Willinger, Sarah Goldberg, Roee Amit(PCP and QCP) were cloned into the RBP plasmid between restriction sites KpnI and AgeI (NEB, catalog: #R3142L and #R3552L respectively), immediately upstream of an mCherry gene lacking a start codon, under the

(17) Zebrafish neuromesodermal progenitors undergo a critical state transition in vivoiScienceOctober 21, 2022Kane Toh, Dillan Saunders, Berta Verd, Benjamin Steventondish,MatTek,P35G-1.5-10-C Inverted confocal Microscope,Leica,SP8 Inverted confocal Microscope,Zeiss,LSM700 KpnI-HF enzyme,NEB,R3142L

(18) Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activityMolecular CellMarch 17, 2022Connor A. Tsuchida et al.Biotech,Cat#A600451-0050 BbsI-HF,NEB,Cat#R3539L AgeI,NEB,Cat#R3552L BamHI,NEB,Cat#R0136L KpnI,NEB,Cat#R3142L PciI,NEB,Cat#R0655L "Dulbecco’s Modified Eagle’s Medium, high glucose",Gibco,Cat#11995073 Opti-MEM I Reduced Serum Medium

(19) Two Compact Cas9 Ortholog-Based Cytosine Base Editors Expand the DNA Targeting Scope and Applications In Vitro and In VivoFrontiers in Cell and Developmental BiologyMarch 1, 2022Susu Wu et al.mRuby with an independent cassette. The reporter was digested with EcoRI (NEB, #R3101L) and KpnI (NEB, #R3142L) enzymes for linearization. Synthesized target oligos including gRNAs, PAMs, and cohesive ends with

(20) The geometric influence on the Cys2His2 zinc finger domain and functional plasticityNucleic Acids ResearchJune 19, 2020April L Mueller, Carles Corbi-Verge, David O Giganti, David M Ichikawa, Jeffrey M Spencer, Mark MacRae, Michael Garton, Philip M Kim, Marcus B Noyesmaxiprep (Qiagen:12963) of 1L overnight culture. The recovered DNA was digestion with kpnI-HF (NEB:R3142L) and XbaI (NEB:R0145L), followed by gel isolation using minelute gel extraction kits (QIAGEN:28606).

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KpnI-HF

KpnI-HF from New England Biolabs

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New England Biolabs

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KpnI-HF from New England Biolabs

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