DNA Polymerase I, Large (Klenow) Fragment from New England Biolabs

Product Specs
ItemDNA Polymerase I, Large (Klenow) Fragment
CompanyNew England Biolabs
Price $271.00 Inquire
Catalog NumberM0210M
Concentration50,000 units/ml
Quantity1000 units
ApplicationsDNA Manipulation
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmol of dNTPs to an acid-insoluble form in 30 minutes at 37°C.

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A plastic EMP1⁺ to LGR5⁺ cell state conversion as a bypass to KRAS-G12D pharmacological inhibition in metastatic colorectal cancerCancer DiscoveryFebruary 6, 2026Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.μL 0.4 mmol/L biotin-dATP (Thermo Fisher Scientific, 19524016), and 4.5 μL 50,000 U/µL Klenow (NEB, M0210M) in water] and incubated for 45 minutes at 37°C. Quenching was done for 10 minutes at 65°C, and DNA

(2) Direct measurement of sub-kilobase chromatin structure reveals that linker histone H1 broadly compacts chromatin, with differential impact amongst epigenetic statesbioRxivDecember 26, 2025Hera Canaj, Irene Duba, Andres Mansisidor, Andrew Scortea, Ryan Johnson, Hugo Pinto, Arnold Ou, Nicole Pagane, Juhee Pae, Dmitry Fyodorov, et al.washed with MB228 then end repaired and end labeled with PNK (NEB M0201L) and Klenow polymerase (NEB M0210M) with biotinylated dATP and dCTP. After enzyme inactivation with EDTA and incubation at 65°C, the sample

(3) A Plastic EMP1+ to LGR5+ Cell State Conversion as a Bypass to KRASG12D Pharmacologic Inhibition in Metastatic Colorectal CancerCancer DiscovOctober 21, 2025Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.μL 0.4 mmol/L biotin-dATP (Thermo Fisher Scientific, 19524016), and 4.5 μL 50,000 U/µL Klenow (NEB, M0210M) in water] and incubated for 45 minutes at 37°C. Quenching was done for 10 minutes at 65°C, and DNA

(4) BMAL1-TRIM28 represses transposable elements independently of CLOCK in pluripotent cellsNature CommunicationsSeptember 10, 2025Amador Gallardo, Efres Belmonte-Reche, María Marti-Marimon, Joan Domingo-Reinés, Guillermo Peris, Lourdes López-Onieva, Iván Fernández-Rengel, Jiajun Xie, Pablo Tristán-Ramos, Nicolas Bellora, et al.with 50 nM biotin-dATP (Invitrogen, #10484552) and 50U of Klenow polymerase (New England Biolabs, #M0210M), proximity ligation was carried out overnight at 16 °C with 10000U of T4 DNA Ligase (New England Biolabs,

(5) Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencingNucleic Acids ResJanuary 16, 2025Sagnik Sen, Pierre-Olivier Estève, Karthikeyan Raman, Julie Beaulieu, Hang Gyeong Chin, George R Feehery, Udayakumar S Vishnu, Shuang-Yong Xu, James C Samuelson, Sriharsa Pradhanrespectively) with biotin-14-dATP (ThermoFisher Scientific # 19 524 016) and 50 U of DNA polymerase I (NEB # M0210M) was added and incubated for 3 h at RT. Ligase mix was incubated for 16 h at RT. Ligase mix contains

(6) Long non-coding RNAs direct the SWI/SNF complex to cell type-specific enhancersNat CommunJanuary 2, 2025James A. Oo, Timothy Warwick, Katalin Pálfi, Frederike Lam, Francois McNicoll, Cristian Prieto-Garcia, Stefan Günther, Can Cao, Yinuo Zhou, Alexey A. Gavrilov, et al.PNK (NEB, M0201). DNA ends were then blunted using T4 DNA polymerase (NEB, M0203L) and Klenow (NEB, M0210M) and then A-tailed using Klenow (exo-) (NEB, M0212M). RNA 3’ OH ends were ligated with the 5’ rApp ends

(7) Magnetically Functionalized Hydrogels for High‐Throughput Genomic ApplicationsAdvanced Materials TechnologiesJanuary 22, 2024Evan Lammertse, Siran Li, Jude Kendall, Catherine Kim, Patrick Morris, Nissim Ranade, Dan Levy, Michael Wigler, Eric BrouzesμL H2O, 100 μL NEBuffer 2, 60 μL 10mM dNTP (11814362001, Sigma Aldrich), and 10 μL DNA Polymerase I (M0210M, New England Biolabs). The suspension was incubated at room temperature for 1.5 hours and then at 37°C

(8) Condensin positioning at telomeres by shelterin proteins drives sister-telomere disjunction in anaphaseeLifeNovember 21, 2023Léonard Colin, Celine Reyes, Julien Berthezene, Laetitia Maestroni, Laurent Modolo, Esther Toselli, Nicolas Chanard, Stephane Schaak, Olivier Cuvier, Yannick Gachet, et al.biotin-14-dATP (cat. 19524016, Thermo Fisher), dTTP, dCTP, and dGTP, and 50 units of DNA Klenow I (cat. M0210M, NEB) for 45 min at 37°C. Samples were diluted in 8 ml of T4 DNA ligase buffer 1× and incubated 8 hr

(9) Condensin positioning at telomeres by shelterin proteins drives sister-telomere disjunction in anaphaseElifeNovember 21, 2023Léonard Colin, Celine Reyes, Julien Berthezene, Laetitia Maestroni, Laurent Modolo, Esther Toselli, Nicolas Chanard, Stephane Schaak, Olivier Cuvier, Yannick Gachet, et al.biotin-14-dATP (cat. 19524016, Thermo Fisher), dTTP, dCTP, and dGTP, and 50 units of DNA Klenow I (cat. M0210M, NEB) for 45 min at 37°C. Samples were diluted in 8 ml of T4 DNA ligase buffer 1× and incubated 8 hr

(10) H3K9 trimethylation in active chromatin restricts the usage of functional CTCF sites in SINE B2 repeatsGenes & DevelopmentApril 1, 2022Francesco Gualdrini, Sara Polletti, Marta Simonatto, Elena Prosperini, Francesco Pileri, Gioacchino NatolidTTP, 37.5 μL of 0.4 mM Biotin-11-dATP, 1 μL of 50 U/μL DNA polymerase I large [Klenow] fragment [NEB M0210M]) was added and incubated at 750 rpm for 45 min at 37°C. After enzyme inactivation at 65°C and centrifugation

(11) Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cellsNeuronFebruary 2, 2022Franjic D, Skarica M, Ma S, Arellano JI, Tebbenkamp ATN, Choi J, Xu C, Li Q, Morozov YM, Andrijevic D, et al.,Sino Biological,Cat#13030-H09E Digoxigenin-UTP,Roche,Cat#11209256910 BsrGI,New England BioLabs,Cat#R0575L PacI,New England BioLabs,Cat#R0547L Klenow,New England BioLabs,Cat#M0210M "Trypsin Gold, Mass Spectrometry Grade",Promega,Cat#V5280 PolyJet,SignaGen,Cat#SL100688 Protamine

(12) Pax7 pioneer factor action requires both paired and homeo DNA binding domainsNucleic Acids ResearchJuly 21, 2021Audrey Pelletier, Alexandre Mayran, Arthur Gouhier, James G Omichinski, Aurelio Balsalobre, Jacques DrouinmCi/mL, Perkin Elmer) and 1 unit of DNA polymerase I Large (Klenow) fragment from E. coli (NEB, catalog # M0210M). Probes were then purified on Micro Bio-Spin P-30 Tris chromatography column (Bio-rad, catalog # 732-6223). Nuclear

(13) A gene-environment-induced epigenetic program initiates tumorigenesisNatureFebruary 1, 2021Alonso-Curbelo D, Ho YJ, Burdziak C, Maag JLV, Morris JP 4th, Chandwani R, Chen HA, Tsanov KM, Barriga FM, Luan W, et al.Xho/EcoRI-HF or XhoI/BamHI-HF restriction enzymes (New England Biolabs), respectively followed by Klenow step (M0210M, New England Biolabs), gel extraction purification of the digested back-bone fragment (QIAquick Gel

(14) Design and Construction of a Focused DNA-Encoded Library for Multivalent Chromatin Reader ProteinsMoleculesFebruary 22, 2020Justin M. Rectenwald et al.E0553L), T4 DNA Ligase (catalog number M0202M), and DNA Polymerase I, Large (Klenow) Fragment (catalog M0210M) were obtained from New England Biolabs, Inc. (Ipswich, MA, USA). SsoAdvanced™ Universal SYBR® Green Supermix

(15) Suppressing proteasome mediated processing of topoisomerase II DNA-protein complexes preserves genome integrityeLifeFebruary 14, 2020Sciascia N, Wu W, Zong D, Sun Y, Wong N, John S, Wangsa D, Ried T, Bunting SF, Pommier Y, et al.protein",T4 DNA polymerase,NEB,Cat# M0203L,15 U "Peptide, recombinant protein",Klenow fragment,NEB,Cat# M0210M,5 U "Peptide, recombinant protein",T4 polynucleotide kinase,NEB,Cat# M0201L,15 U "Peptide, recombinant

(16) Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during SpermatogenesisCell ReportsJuly 9, 2019Covadonga Vara et al.,New England Biolabs,#R0147M Biotin-14-dATP,Life Technologies,#19524-016 "DNA Polymerase I, large (Klenow) Fragment",New England Biolabs,#M0210M T4 DNA Ligase,New England Biolabs,#M0202M Dynabeads MyOne Streptavidin T1,Life Technologies,#65001 T4

(17) A streamlined tethered chromosome conformation capture protocolBMC GenomicsDecember 1, 2016Idan Gabdank, Sreejith Ramakrishnan, Anne M. Villeneuve, Andrew Z. Fire

(18) Chromosome-scale shotgun assembly using an in vitro method for long-range linkageGenome ResearchMarch 1, 2016Nicholas H. Putnam et al.in by incubating with dNTPs, including a-S-dGTP and biotinylated dCTP along with 25 U of Klenow (no. M0210M, NEB) in 165 μL total volume at 25°C for 40 min. The fill-in reaction was stopped by adding 7 μL of

Be the First to Write a Review!