DNA Polymerase I, Large (Klenow) Fragment from New England Biolabs

Product Specs
ItemDNA Polymerase I, Large (Klenow) Fragment
CompanyNew England Biolabs
Price $271.00 Inquire
Catalog NumberM0210L
Quantity1000 units
Concentration5 U/µl
ApplicationsDNA Manipulation
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmoles of dNTPs to an acid-insoluble form in 30 minutes at 37°C.

Comments:
DNA sequencing by the Sanger dideoxy method Fill-in of 3´ recessed ends Second-strand cDNA synthesis Random priming labeling. Second strand synthesis in mutagenesis protocols.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Functional and Computational Interrogation of the Juvenile Idiopathic Arthritis Risk Loci Identifies Candidate Risk-Driving SNPs and Target Genes in CD4+ T CellsHGG AdvApril 28, 2026Kaiyu Jiang, Emma K. Haley, Gilad Barshad, Adam He, Anita Rogic, Edward Rice, Marc Sudman, Susan D. Thompson, David A. Murphy, Yao Fu, et al.Following dephosphorylation with rSAP and end polishing using T4 PNK, DNA polymerase Klenow fragment (NEB #M0210), and biotinylated dATP and dCTP, we performed ligation in a final volume of 2.5 mL for 3 h at room

(2) A low-input Micro-C protocol for high-resolution 3D genome mappingBiology Methods and ProtocolsApril 15, 2026Fengnian Shan, Chongren Pei, Sijian Xia, Fei Ling1.33 μl of T4 polynucleotide kinase (10 U/μl, NEB, M0201L), 1.33 μl of Klenow fragment (5 U/μl, NEB, M0210L), 1.33 μl of T4 DNA ligase (30 U/μl, Thermo, EL0013), 0.6 μl of BSA (20 mg/ml, BBI, B600036-0001), and

(3) Distinct mechanisms of replication stress induced by oncogenic RAS and cyclin E1 converge on R-loop-dependent fork reversalNature CommunicationsApril 3, 2026Anna Oravetzova, Marketa Dvorakova, Anca-Irina Mihai, Martin Andrs, Margarita Sobol, Anton Zuev, Kaustubh Shukla, Barbora Boleslavska, Vinicio Rosano, Christiane König, et al.vector was religated by T4 DNA ligase (NEB, M0202S) after filling in DNA ends with Klenow Fragment (NEB, M0210S). pBABEneo-CCNE1 was constructed by inserting the Bsp1407I/HindIII fragment of pLXSNneo-CCNE1 (kindly

(4) Stage-specific epigenetic priming amplifies gene activation during lineage commitmentSci AdvMarch 27, 2026Arion Foertsch, Naama Hirsch, Janine Seyfferth, Ward Deboutte, Herbert Holz, Maria Shvedunova, Remzi Karayol, Thomas Stehle, Laura Arrigoni, Niyazi Umut Erdogdu, et al.at 37°C for 15 min with interval mixing. Then, 50 U of the DNA Polymerase I Klenow Fragment (NEB, #M0210) was added to the reaction and incubated at 37°C for 15 min with interval mixing. Next, the following

(5) Chromatin reorganization drives overexpression of a Btaf1 variant underpinning hematopoietic agingNature CommunicationsMarch 18, 2026Le Zong, Bongsoo Park, Yaqiang Cao, Fei Ma, Ferda Tekin-Turhan, Wakako Kuribayashi, Keji Zhao, Isabel BeermanThermoFisher), 0.75 μl of 10 mM dGTP (18254011, ThermoFisher), 4 μl of DNA Polymerase I Large (Klenow) Fragment (M0210L, NEB) was added, and incubated in a thermomixer at 37 °C for 1.5 h with 1,000 rpm mixing. 450 μl of

(6) A Translocation within the Ogataea Species Complex Alters Local Subtelomeric Chromatin while Maintaining Overall Genome OrganizationbioRxivMarch 12, 2026Tiffany J. Lundberg, Nickolas M. Lande, Daniel Tourevski, Riley Figueroa, Sara J. Hanson, Andrew D. Klockomade porous, the chromatin was digested by DpnII (NEB cat# R0543L), end-repaired with Klenow (NEB cat# M0210L) and dNTPs, including biotin-14-dATP (Invitrogen cat# 19524–016), and ligated with T4 DNA Ligase (NEB

(7) In vitro reconstitution of heterochromatin compartments reveals the spontaneous formation of tunable liquid-liquid interfacesNucleic Acids ResMarch 10, 2026Lucy D Brennan, Hyeong-Ku Kim, Serafin Colmenares, Tatum Ego, Je-Kyung Ryu, Gary KarpenEcoRV was heat-inactivated per NEB instructions, and the DNA was end labeled with Klenow polymerase (M0210S, NEB) using either Cy5-dCTP or Cy3-dUTP. Labeled DNA was ethanol precipitated and resuspended in 1×

(8) Retrotransposon activation during spermatogenesis achieves massive ecDNA biogenesis, but rare integrationGenes & DevelopmentMarch 2, 2026Lauren Tracy, Yao Chen, Zz Zhao Zhang(Thermo Fisher Scientific Cat. # 18021014) and the second strand of cDNA was synthesized by DNA Polymerase I (NEB Cat. # M0210L) with dUTP incorporation. The DNA was purified with AMPure XP beads. Then the DNA ends were repaired,

(9) Identification of a cytosol-to-nucleus feedback loop that regulates neuronal microtubule nucleationJournal of Cell BiologyMarch 2, 2026Nitish Kumar, Nathaniel Carey, J. Ian Hertzler, Annabelle R. Bernard, Gibarni Mahata, Gregory O. Kothe, Yitao Shen, Melissa M. Rollsligase (NEB M0202S). Fill-in reactions for blunting were carried out with Large Klenow fragment (NEB M0210S). Agarose gel purification was carried out by standard methods using Gel and PCR Clean-up Nucleospin

(10) Aub, Vasa, and Armi concentration in phase-separated nuage is dispensable for piRNA biogenesis and transposon silencingRNAMarch 1, 2026Samantha Ho, Nicholas P. Rice, Tianxiong Yu, Zhiping Weng, William E. TheurkaufN0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3′ to 5′ exo,NEB,Cat# M0212L UDG,NEB,Cat# M0280S Phusion Polymerase,NEB,Cat#

(11) Rapid disassembly and Piwi-independent reassembly of Drosophila piRNA cluster heterochromatin following acute heat shockRNAMarch 1, 2026Nicholas P. Rice, Samantha Ho, Zhiping Weng, William E. TheurkaufN0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3′ to 5′ exo,NEB,Cat# M0212L UDG,NEB,Cat# M0280S Phusion polymerase,NEB,Cat#

(12) Loop Extrusion Accelerates Long-Range Enhancer-Promoter Searches in Living EmbryosbioRxivFebruary 21, 2026Pavan Choppakatla, Aleena L. Patel, Tohn Borjigin, Tee Udomlumleart, Jie Hu, Thomas Gregor, Alistair N. Boettiger, Michael S. LevineATP, 2 mM DTT) for 15 min at 37 °C and then 25 U of large Klenow fragment of DNA Polymerase I (NEB #M0210) was added and the mixture was incubated for a further 15 min at 37 °C. These DNA ends were then biotin

(13) NSD3 stabilizes nuclear compartmentalization and promotes megabase-scale chromatin interactionsbioRxivFebruary 14, 2026Yi-Hung Chen, John R. Collette, Krupa Sampat, Ivaylo D. Yonchev, Catherine E. Hawkins, Christopher Ponne, Celeste D. Rosencrance, Kyle P. EagendGTP, 1.65 μL 10 mM dCTP, 8.8 μL 5 U/mL DNA Polymerase I, Large (Klenow) Fragment (New England Biolabs #M0210)) and incubated at 37° C for 45 min while shaking at 750 rpm, 15 s on, 4 min 45 s off in a ThermoMixer.

(14) Differential nucleosome organization in human interphase and metaphase chromosomesMolecular Systems BiologyFebruary 2, 2026Keren Li, Irem Unlu, Yiren Tu, Lilien N Voong, Yanyan Lu, Brody Kendall, Xiaotian Ma, Sin Lei Pui, Meng Tao, Ji-Ping Wang, et al.Fisher,AAA1149306 Nocodazole,Sigma,SML1665-1ml DNATerminator® End Repair Kit,Lucigen,40035-2 Klenow DNA Polymerase I,NEB,M0210S Micrococcal nuclease,Sigma,N3755-500UN 3-mercaptopropionic acid,Sigma,M5801-100G Neocuproine,Sigma,N1501-1G Copper(II) chloride dihydrate

(15) An integrated multi-omics and network analysis of neutrophil differentiation from initial- to late-stageGenome BiologyJanuary 30, 2026Qing Chen, Tianchang Yang, Yingping Hou, Kai Wang, Tingting Li, Longteng Wang, Cuiyun Dou, Panpan Cheng, Minglei Shi, Wei Licontaining 1.5 μL 5 mM dNTPs, 3.5 μL 10 × NEBuffer 3.1, 5 μL DNA Polymerase I (Klenow fragment, NEB M0210L), and 25 μL H₂O. The mixture was incubated at 37 °C for 2 ~ 3 h with rotation.Ligation was performed

(16) Neural stem cell quiescence is actively maintained by the epigenomeCell ReportsJanuary 27, 2026Anna Malkowska, Jan Ander, Andrea H. Brandfree),Roche,11119915001 DpnI,NEB,R0176 DpnII,NEB,R0543S Klenow 3′–5′ exo-enzyme,NEB,M9212L Klenow Fragment (5 U/μL),NEB,M0210S MyTaq HS DNA Polymerase,Bioline,BIO-21113 NEBNext High Fidelity 2 × PCR Master Mix (6.25 mL),NEB,M0541L PEG-8000,Sigma-Aldrich,P5413 T4 DNA Ligase

(17) Single-cell multiome and enhancer connectome of human retinal pigment epithelium and choroid nominate causal variants in macular degenerationCell ReportsJanuary 27, 2026Sean K. Wang, Jiaying Li, Surag Nair, Reshma Kosaraju, Yun Chen, Yuanyuan Zhang, Anshul Kundaje, Yuwen Liu, Ningli Wang, Howard Y. Chang,New England BioLabs,Cat# R0147 Biotin-14-dATP,Jena Bioscience,Cat# NU-835-BIO14 Klenow fragment,New England BioLabs,Cat# M0210 T4 DNA ligase,New England BioLabs,Cat# M0202 Dimethylformamide,Sigma-Aldrich,Cat# 227056 Sodium deoxycholate,Bioworld,Cat#

(18) Gene mobility elements mediate cell type specific genome organization and radial gene movement in vivobioRxivJanuary 16, 2026Tanguy Lucas, Lin-Ing Wang, Juniper Glass-Klaiber, Elvis Quiroz, Sofiya Patra, Natalia Molotkova, Minoree KohwidNTP mix (dC/G/TTP, NEB, N0446) and 0.8ml of 5U/μl DNA Polymerase I, Large (Klenow) Fragment (NEB, M0210) by incubating at 37°C for 1.5hrs at 300 rpm, shaking. Following biotinylation, 1ml of cold freshly

(19) Predictive prioritization of enhancers associated with pancreatic disease riskCell GenomJanuary 14, 2026Wang L, Baek S, Prasad G, Wildenthal J, Guo K, Sturgill D, Truongvo T, Char E, Pegoraro G, McKinnon K, et al.at a final concentration of 40 μM each) and 15U of DNA Polymerase I, Large (Klenow) Fragment (NEB, M0210) were added and incubated at 37°C for 45 min. Ends were ligated by adding 484 μL of ligation mixture

(20) Pooled CRISPRi screening reveals fungal-specific vulnerabilities across environments and genetic backgroundsbioRxivJanuary 10, 2026Lauren F Wensing, Philippe C Després, Desiree Francis, Meea Fogal, Anthony Hendriks, Nicholas C Gervais, Clara Fikry, Abdul-Rahman Adamu Bukari, Aleeza C Gerstein, Christina A Cuomo, et al.°C and hold for 1 min. Then, 1 μL of 5,000 U/mL DNA polymerase I, Large (Klenow) fragment (NEB, cat. M0210S) and 1 μl of 10 mM dNTPs (NEB, cat. N0447L) was added to the tube. The thermocycler conditions were

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