T4 Polynucleotide Kinase Reaction Buffer from New England Biolabs

Product Specs
ItemT4 Polynucleotide Kinase Reaction Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB0201S
Quantity4 ml
ApplicationsDNA Manipulation
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modificationsNature CommunicationsMarch 23, 2026Yuru Wang, Kinga Pajdzik, Yutao Zhao, Chang Ye, Talbot Stone, Li-Sheng Zhang, Wen Zhang, Mahdi Assari, Wenxin Zhao, Caraline Sepich-Poore, et al.water, and all of it was used in a 50 µL scale end repair reaction containing 1× T4 PNK buffer (NEB B0201S) and 5 µL T4 PNK (Thermos Fisher) with incubation at 37 °C for 1 h. The RNA was then purified using

(2) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. BartelPolynucleotide Kinase (PNK) (NEB, M0201) and [γ-32P] ATP (Revvity, BLU035C005MC) in T4 PNK reaction buffer (NEB, B0201S) at 37 °C for 1–2 h, then desalted using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221) and gel-purified

(3) Stepwise DNA unwinding gates TnpB genome-editing activitybioRxivJanuary 28, 2026Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, et al.M0201S). RNA samples were incubated in a 20 μL reaction containing T4 PNK buffer (New England Biolabs, B0201S) and 1 μL QuickCIP at 37 °C for 30 min, followed by heat-inactivation at 85 °C for 10 min. Without additional

(4) Conserved shifts in sperm small non-coding RNA profiles during mouse and human agingThe EMBO JournalJanuary 20, 2026Junchao Shi, Xudong Zhang, Chen Cai, Shichao Liu, Jiancheng Yu, Emma R James, Lihua Liu, Benjamin R Emery, Megan R McMurray Bires, Elizabeth Torres-Arce, et al.(nt) were incubated in a 50 μL reaction mixture containing 5 μL 10× PNK buffer (New England Biolabs; B0201S), 1 mM ATP (New England Biolabs; P0756S), 10 U T4PNK (New England Biolabs; M0201L) at 37 °C for 20 min.

(5) ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesisNat CommunOctober 13, 2025Yu Qian, Canlan Wu, Saisai Wei, Sujun Yan, Junxuan Peng, Lei Yu, Yunyi Gao, Jingyu Hou, Wentao Yu, Zhanghui Chen, et al.and the ends of the protected fragments were phosphorylated using T4 polynucleotide kinase (T4 PNK; B0201S, NEB). Sequencing libraries were generated with the Small RNA Library Prep Set for Illumina (E7330S,

(6) Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolutionNat CommunOctober 6, 2025Cheng-Wei Ju, Han Li, Bochen Jiang, Xuanhao Zhu, Liang Cui, Zhanghui Han, Junxi Zou, Yunzheng Liu, Shenghai Shen, Hardik Shah, et al.Thermo Fisher Scientific, EK0032). For this reaction, RNA was combined with 3 µL of 10× T4 PNK reaction buffer (NEB, B0201S) and 3 µL T4 PNK in a final volume of 30 µL, incubated at 37 °C for 45 min, then supplemented with 1.5

(7) Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell‐Induced Ferroptosis in Ovarian CancerAdvanced ScienceOctober 3, 2025Jiakai Hou, Cheng‐Wei Ju, Nicholas A. Egan, Yanjun Wei, Yunfei Wang, Minghao Dang, Tianyi Zhou, Leilei Shi, Ningbo Zheng, Si Chen, et al.incubating with the buffer containing 3 µL T4 PNK (3M0201S, New England Biolabs), 3 µL PNK buffer (#B0201S, New England Biolabs), 1.5 µL RNase inhibitor (#AM2696, Invitrogen) at 37 °C for 60 min. Then, 1.5 µL

(8) Promoter- and enhancer-dependent cohesin loading initiates chromosome looping to fold Tcrb loci for long-range recombinationCell ReportsAugust 26, 2025Brittney M. Allyn, Katharina E. Hayer, Clement Oyeniran, Vincent Nganga, Kyutae Lee, Ahmet Sacan, Eugene M. Oltz, Craig H. BassingT4 Polynucleotide Kinase,New England Biolabs,Cat# M0201S T4 Polynucleotide Kinase Buffer,New England Biolabs,Cat# B0201S Dithiothreitol (DTT),Fisher Scientific,Cat# BP172-5 Fragmentation Reagents,Invitrogen by ThermoFisher Scientific

(9) An Alternative Gene Editing Strategy Using a Single AAV VectorBio ProtocJuly 5, 2025Jamie C. Moffa, Vani Kalyanaraman, Bryan A. Copits(New England BioLabs, catalog number: P0756S) 8. 10× PNK T4 buffer (New England BioLabs, catalog number: B0201S) 9. T4 PNK (New England BioLabs, catalog number: M0201L) 10. 2× T7 buffer (New England BioLabs, catalog

(10) An Alternative Gene Editing Strategy Using a Single AAV VectorBIO-PROTOCOLJuly 5, 2025Jamie C. Moffa, Vani Kalyanaraman, Bryan A. Copits(New England BioLabs, catalog number: P0756S) 8. 10× PNK T4 buffer (New England BioLabs, catalog number: B0201S) 9. T4 PNK (New England BioLabs, catalog number: M0201L) 10. 2× T7 buffer (New England BioLabs, catalog

(11) Small-molecule-catalysed deamination enables transcriptome-wide profiling of N6-methyladenosine in RNANature ChemistryJuly 1, 2025Pingluan Wang, Chang Ye, Michelle Zhao, Bochen Jiang, Chuan Hepolynucleotide kinase (PNK, NEB, M0201S). RNA was combined with 2 μl of 10× T4 PNK reaction buffer (NEB, B0201S) and 3 μl T4 PNK, diluted to a final volume of 20 μl and incubated at 37 °C for 60 min. The mixture

(12) Comprehensive analysis of tRNA-derived fragment expression in endometriosis using PANDORA-seq technologyRNA BiologyJune 27, 2025Jingyao Huang, Junping Cheng, Dandan Zhang, Ying Wang, Huanying Liang, Liling Liufresh 50 μL reaction mixture containing 5 μL of 10× PNK buffer (New England Biolabs; catalogue number B0201S), 10 mM ATP (New England Biolabs; catalogue number P0756S), and 10 U of T4PNK (New England Biolabs;

(13) Generation of iPSC Lines with Tagged α-Synuclein for Visualization of Endogenous Protein in Human Cellular Models of Neurodegenerative DisorderseNeuroJune 2, 2025Oskar G. Zetterdahl, James A. Crowe, Samira Reyhani, Miriam A. Güra, Ot Labastida-Botey, Aline S. Girard, D. Sean Froese, Henrik Ahlenius, Isaac CanalsEngland Biolabs #B0201SVIAL) and 6.5 µl of ddH20 with 0.5 µl of T4 PNK enzyme (New England Biolabs #B0201S). The reaction was allowed to proceed in a thermocycler at 37°C for 5 min followed by 95°C for 5 min,

(14) Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell deathNat CommunJune 2, 2025Matthew B. Hanlon, Jason M. Shohet, Scot A. Wolfeoligo, 5 µL reverse oligo, 5 µL T4 polynucleotide kinase reaction buffer (10x; New England Biolabs, #B0201S), and 35 µL ddH2O. Mixture was incubated at 95 °C for 4 min and left to cool to room temperature. After

(15) Rainbow Nucleus Charts Dynamic Interactome of Membrane-less OrganellesBIORXIVMay 15, 2025Songtao Ye, Nadav Benhamou Goldfajn, Choon Leng So, Takanari Inoue, Danfeng CaiμL 100 μM reverse oligo, 1 μL 10x T4 ligation buffer (B0202S, NEB), 1 μL T4 polynucleotide kinase (B0201S, NEB), and 6 μL nuclease-free water. The mixtures were incubated at 37°C for 30 min, heated to 95°C

(16) Protocol for the functional evaluation of genetic variants using saturation genome editingSTAR ProtocApril 8, 2025Sofia Obolenski, Rebeca Olvera-León, Dijue Sun, David J. Adams, Andrew J. Waters"T4 polynucleotide kinase (10,000 U/mL)",NEB,Cat#M0201L T4 polynucleotide kinase reaction buffer (10X),NEB,Cat#B0201S Quick ligase,NEB,Cat#M2200S Quick Ligation reaction buffer (2X),NEB,Cat#B2200S KAPA HiFi HotStart ReadyMix

(17) Transfer RNA acetylation regulates in vivo mammalian stress signalingSci AdvMarch 21, 2025Supuni Thalalla Gamage, Roxane Khoogar, Shereen Howpay Manage, Judey T. DaRos, McKenna C. Crawford, Joe Georgeson, Bogdan V. Polevoda, Chelsea Sanders, Kendall A. Lee, Kellie D. Nance, et al.M0201L) at 37°C for 1 hour in the presence of T4 PNK buffer without adenosine triphosphate (ATP) (NEB, B0201S). For tRNA-seq libraries, a previously described 5′-phosphorylated, preadenylated adapter (oBZ407) with

(18) Bacteria exploit viral dormancy to establish CRISPR-Cas immunityCell Host & MicrobeMarch 12, 2025Nicholas C. Keith, Rhett A. Snyder, Chad W. Euler, Joshua W. ModellT4 Polynucleotide Kinase,NEB,Cat # M0201S T4 DNA Ligase,NEB,Cat # M0202S T4 Polynucleotide Kinase Reaction Buffer,NEB,Cat # B0201S BsaI-HFv2,NEB,Cat # R3733S Phosphate Buffered Saline,Corning,46-013-CM DNaseI,NEB,Cat # M0303S Rnase A

(19) Small RNA data sets of mouse testes and ovaries before and after sexual maturitySci DataFebruary 27, 2025Mailin Gan, Xingyu Wang, Jianfeng Ma, Lei Chen, Yan Wang, Linyuan Shen, Li ZhuT4PNK The RNA was incubated in 50 μl reaction mixture containing 5 μl 10 × PNK buffer (New England Biolabs; B0201S), 10 mM ATP (New England Biolabs; P0756S), 10 U T4PNK (New England Biolabs;M0201L) and 200 ng RNA at

(20) TRMT1L-catalyzed m22G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stressCell ReportsJanuary 28, 2025Sseu-Pei Hwang, Han Liao, Katherine Barondeau, Xinyi Han, Cassandra Herbert, Hunter McConie, Amirtha Shekar, Dimitri G. Pestov, Patrick A. Limbach, Jeffrey T. Chang, et al.buffer,Invitrogen,AM2238 T4 polynucleotide kinase,NEB,M0201S T4 polynucleotide kinase reaction buffer,NEB,B0201S T4 RNA ligase,NEB,M0204S T4 RNA ligase 2,NEB,M0239S Leupeptin,Fisher,PI78435 [γ-32P]ATP,Perkin-Elmer,NEG502A250UC Nitrocellulose membrane

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