Kinase Buffer (10X) from Cell Signaling Technology

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ItemKinase Buffer (10X)
CompanyCell Signaling Technology
Price $89.00 Inquire
Catalog Number9802S
Quantity15 ml
Research AreaRelated Products

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
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Website: http://www.cellsignal.com/

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(1) FGF1 orchestrates circadian hepatic triglyceride secretionNature CommunicationsMarch 19, 2026Benan Pelin Sermikli, Sihao Liu, Kyeongkyu Kim, Linnea Hases, Tim van Zutphen, Ashley Untereiner, Jocelyn Torres, Mingxiao He, Lillian Crossley, Yang Dai, et al.recombinant IRE1α was incubated with 250 ng of recombinant mTORC1 in kinase buffer (Cell Signaling, # 9802S) containing 25 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM DTT, and 100 μM ATP (Cell Signaling #9804S) in a

(2) A hotspot phosphorylation site on SHP2 drives oncoprotein activation and drug resistanceNature CommunicationsMarch 3, 2026Prashath Karunaraj, Remkes Scheele, Malcolm L. Wells, Ruchita Rathod, Ipek S. Gokulu, Sophia R. Abrahamson, Lila Taylor, Lamia Chowdhury, Abiha Kazmi, Weixiao Song, et al.SinoBiological. Reaction mixture was prepared with the 10x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1

(3) Targeting leucine-rich repeat kinase 2 overcomes resistance to oncolytic herpes simplex virus-based therapies in glioblastomaNature CommunicationsFebruary 28, 2026Yaning Qin, Zhiqi Liang, Mengqin Yu, Nanqian Lin, Naizhen Wang, Xuanke Huang, Shi Chen, Qun Peng, Yanhua Lin, Heqin Wang, et al.LRRK2 protein (Thermo Fisher Scientific, A15197) in 1 × kinase buffer (Cell Signaling Technologies, 9802S) containing 200 µM ATP (Cell Signaling Technologies, 9804S) for 30 min at 30 °C. The reaction products

(4) Targeting the FOSL1/IKKα positive feedback loop attenuates glioblastoma malignancy via suppression of NF-κB signalingTheranosticsJanuary 1, 2026Beichen Zhang, Hao Tian, Haoyu Zhou, Yichang Wang, Ke Gao, Yiyang Cao, Mingjing Zhou, Maode Wang, Wei Wu, Jia WangIKKα (P5564, Abnova) were incubated in a 30 µl reaction mixture containing 20 µl of kinase buffer (#9802, Cell Signaling Technology) and 200 µM ATP. The kinase reaction was carried out at 30 °C for 2 h and

(5) AAK1 activation-mediated iron trafficking drives ferroptotic cell deathNature CommunicationsDecember 17, 2025Li-Chao Li, Zhi-Peng Ye, Ying Xiao, Xian-Ying Zhu, Qia-Qia Li, Yi-Qing Guo, Huai-Liang Wu, Zhi-Ling Li, Lin-Yu Wu, Yu-Hong Chen, et al.incubated with human recombinant activated PKCβII kinase (Abcam, ab60841) in 1 × kinase buffer (CST, 9802S) in the addition of 400 μM ATP (CST, 9804S) with or without 0.5 μl λ-phosphatase at 30 °C for 1 h. Negative

(6) Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progressionNature CommunicationsDecember 10, 2025Silu Sun, Bing Zhong, Ying Wang, Zaiye Li, Yuchen Jiang, Ning Ji, Xiaodong Feng, Rui Liu, Xin Zeng, Xikun Zhou, et al.Tris-HCl (pH 7.5), 5 mM β-glycerophosphate, 2 mM dithiothreitol, 0.1 mM Na3VO4 and 10 mM MgCl2) (CST, #9802) for 30 min at 30 °C. The reaction was subsequently terminated by adding SDS–PAGE sample loading buffer

(7) Targeting TNK2/ACK1 reverses the immunosuppressive tumor microenvironment and synergizes with immunochemotherapy in pancreatic cancerNature CommunicationsDecember 6, 2025Chao Wu, Weishuai Liu, Xiangting Hu, Yongjie Xie, Shengnan Li, Xinyue Liu, Zhaojun Sun, Xiaoling Li, Xin Yu, Yudong Yuan, et al.TNK2-wt, TNK2-ΔN, or TNK2-K158R was incubated with STAT5a, respectively in kinase reaction buffer (CST, #9802) containing 200 μM ATP (CST, #9804) on ice. After 30 min of incubation at 30 °C, SDS sample loading

(8) AMPK phosphorylates WIP1 to promote DNA repair and radioresistance in cancer cellsCell Death & DiseaseNovember 28, 2025Manman Lu, Xiaochuan Dong, Chunrui Wu, Guisong Wang, Haiyang Wang, Yingli Pan, Yali Qin, Yushuai Song, Hongming Pan, Shenzhi Liu, et al.three times with 1 ml of lysis buffer and twice with kinase buffer (Cell Signaling Technology, Cat #: 9802). The immunoprecipitates were resuspended in 50 μl of kinase buffer containing 0.5 mg of GST-Vec and

(9) Aurora kinase B phosphorylates ZBP1 to drive PANoptosis following treatment with PARP and ATR inhibitors combinationNature CommunicationsNovember 27, 2025Wenjian Gong, Zhiqi Liao, Fan Xiong, Linghui Wang, Shennan Shi, Mengshi Luo, Yuewen Zhang, Qiuyang Xu, Yuanyuan Wang, Yijie Wu, et al.0.5 μg of the recombinant ZBP1 protein was incubated with AURKB or CDK1 in the kinase buffer (CST, 9802) together with 1 mM cold ATP (MCE, HY-B2176) for 1 h at 30 °C, which was stopped by adding 5× loading

(10) GSK461364 Inhibits NLRP3 Inflammasome by Targeting NEK7 PhosphorylationAdvanced ScienceNovember 1, 2025Ruiheng Luo, Mingliang Ma, Dan Wang, Ling Luo, Xueming Xu, Lingmin Huang, Fupeng Wang, Guangyan Kuang, Huiqi Liu, Rui Ni, et al.SDS‐PAGE gels. In Vitro Kinase Assays PLK1‐his proteins, NEK7‐GST protein, were subjected to the kinase buffer(9802, CST) in the presence of 200 µm ATP(9804, CST)or without. The reaction mixtures were incubated for 60

(11) Chromatin looping-based CRISPR screen identifies TLK2 as chromatin loop formation regulator in cancer stemness plasticityNat CommunOctober 21, 2025Zifeng Wang, Fang Liu, Nana Chen, Jingjing Wu, Xinhao Li, Mouxiang Fang, Min Yan, Ji Zhang, Bing Deng, Lulu Wang, et al.Sino Biological Inc (Cat# 11551-HNCB, aa 397-772). The kinase reactions were performed in kinase buffer (10×) (Cat# 9802, CST) with 200 μM γ−32P ATP (PerkinElmer), 1 µg of soluble DYNLL1 or ASF1A, and 100 ng of TLK2. The

(12) Phosphorylation of plectin by Akt3 promotes triple-negative breast cancer cell invasive migrationiScienceOctober 17, 2025Shixue Zheng, Chloe Chan, John M. Asara, Liang Zhang, Gerhard Wiche, Y. Rebecca ChinAkt3 (Sigma-Aldrich A0983) in the presence of 250 μM ATP in a kinase buffer (Cell Signaling Technology 9802S). The reaction was terminated by the addition of SDS-PAGE loading buffer and the samples were analyzed

(13) Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entryNatureOctober 2, 2025Debasish Paul, Derek L. Bolhuis, Hualong Yan, Sudipto Das, Xia Xu, Christina C. Abbate, Lisa M. M. Jenkins, Michael J. Emanuele, Thorkell Andresson, Jing Huang, et al.0883-0000-1) or Flag–DYRK4 (Carna Biosciences, 04-434) in 1× kinase buffer (Cell Signaling Technology, 9802). The assay was performed with 100 µM of ATP (Cell Signaling Technology, 9804) at 37 °C for 30 min,

(14) Glycolytic Enzyme HK2 Phosphorylates nSMase1 to Promote Astrocytic Exosomes Biogenesis Contributing to Acute Ischemic Stroke InjuryAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Chen C, Zhou Y, Sheng L, Mai F, Ding Y, Hong S, Wu J, Pi J, Yan G, Huang Y, et al.8179‐HK‐020) with or without lonidamine (100 µm) in 80 µL kinase reaction buffer (1 X Kinase Buffer (CST, 9802), 200 µm ATP (CST, 9804) in the present of protease/phosphatase inhibitor cocktail (TargetMol, C0001)

(15) Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug ResistanceAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Tian Q, Wang J, Li Q, Liu Y, Chen Y, Feng J, Lei ZN, Patel H, Cai CY, Xu Y, et al.Active BCKDK kinase and 4 µg of inactive AKT WT/2A substrates were incubated in 1× kinase buffer (CST, 9802) with 200 µM ATP (CST, 9804) for 3 h at 4 °C. Phosphorylation was assessed by Western blot using p‐AKT

(16) A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug ResistanceResearchSquareAugust 5, 2025Prashath Karunaraj, Prashath Karunaraj, Malcolm L. Scheele, Malcolm Rathod, Ipek Simay Rathod, Sophia Abrahamson, Sophia Abrahamson, Lamia Chowdhury, Lamia Chowdhury, Weixiao Kazmi, et al.SinoBiological. Reaction mixture was prepared with the 5x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1

(17) PAK4 Phosphorylates and Stabilizes MYC to Promote Acute Myeloid LeukemiaCell InsightJuly 31, 2025Ting Xie, Peipei Sun, Hao Huang, Qilong Li, Hudan Liu, Jue JiangS67A mutant) was combined with 1 μg of His-PAK4 in kinase reaction buffer (Cell Signaling Technology, #9802) containing ATP (Thermo Fisher Scientific, #18330019). The reaction mixtures were incubated at 30 °C

(18) Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activityCell Chemical BiologyJuly 17, 2025Flore Nardella, Tiantian Jiang, Lushun Wang, Monica J. Bohmer, Subhoja Chakraborty, John Okombo, Jaeson Calla, Tatiane Macedo Silva, Samuel Pazicky, Jianwei Che, et al.plate. Compounds were incubated at RT for 10 min with 4 μL of the enzyme mix containing kinase buffer (#9802; Cell Signaling, Danvers, MA) supplemented with 2 mM MnCl2, 0.7 μM of PfPK6-His, 2 μg of histone H1

(19) A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug ResistancebioRxivJune 19, 2025Prashath Karunaraj, Remkes Scheele, Malcolm L. Wells, Ruchita Rathod, Sophia Abrahamson, Lila C. Taylor, Ipek S. Gokulu, Lamia Chowdhury, Abiha Kazmi, Weixiao Song, et al.SinoBiological. Reaction mixture was prepared with the 5x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1

(20) White spot syndrome virus immediate-early protein (wsv100) antagonizes the NF-κB pathway to inhibit innate immune response in shrimpPLoS PathogJune 12, 2025Bang Xiao, Fang Kang, Qianqian Li, Junming Pan, Yue Wang, Jianguo He, Chaozheng Liof His-Dorsal-FL protein with ATP (CST, cat. no. 9804S) were added into 10 × buffer (CST, cat. no. 9802S) to obtain a 1 × reaction system. In the control, the ATP was replaced by ddH2O. The mixtures were incubated

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Kinase Buffer (10X) from Cell Signaling Technology

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