KasI from New England Biolabs

Product Specs
ItemKasI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0544L
Concentration1-5 u/µL
Quantity1250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-80 C

Sequence

G/GCGCC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.the PEG-passivated streptavidin-coated surface. The biotinylated DNA was digested using a KasI (NEB R0544S) or ApaI (NEB R0114S) restriction enzyme to generate single-biotin fragments of 45.68 kb and 2.82 kb,

(2) Loss of nuclear envelope bud formation leads to mitophagy initiation in Drosophila musclesAutophagy ReportsDecember 31, 2025Yungui Guo, David Brooks, Ziwei Zhao, Erica Biven, Erika R. GeisbrechtGenomics Resource Center, Bloomington, IN) was linearized by treating with KasI restriction enzyme (R0544S, NEB, Ipswich, MA) in a 37°C water bath for 1 hr. A 1% DNA agarose gel was used to check the digestion

(3) ANKLE1 processes chromatin bridges by cleaving mechanically stressed DNANat CommunDecember 8, 2025Huadong Jiang, Fei He, Nannan Kong, Jie Long, Yu Ching Poon, Rajvee Shah Punatar, Zhichun Xu, Yuanliang Zhai, Stephen C. West, Artem K. Efremov, et al.New England Biolabs: BamHI (R3136), EcoRI (R0101), PvuI (R3150), EcoRV (R3195), HindIII (R0104), KasI (R0544), Nb.BsmI (R0706), Nt.BspQI (R0644), and T4 DNA ligase (M0202). S1 nuclease (EN0321) was obtained from

(4) Large-scale experimental assessment of variant effects on the structure and function of the citrate transporter SLC13A5Sci AdvJune 27, 2025Wen-An Wang, Evandro Ferrada, Christoph Klimek, Tanja Osthushenrich, Aidan MacNamara, Tabea Wiedmer, Giulio Superti-Furgaprotein (EGFP) from attB_EGFP with Nco I–HF (New England Biolabs, R3193S) and Kas I (New England Biolabs, R0544S) double digestion, and the SLC13A5 insert was made from polymerase chain reaction (PCR) amplification

(5) Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidyProceedings of the National Academy of SciencesNovember 5, 2024Leelabati Biswas, Katarzyna M. Tyc, Mansour Aboelenain, Siqi Sun, Iva Dundović, Kruno Vukušić, Jason Liu, Vanessa Guo, Min Xu, Richard T. Scott, et al.linearized with Ssp I (#R0132S, New England Biolabs, Ipswich, MA) and Kif18A plasmids with Kas I (#R0544S, New England Biolabs, Ipswich, MA) restriction enzymes. pGEMHE-mEGFP-mTrim21 (#105519, Addgene, Watertown,

(6) Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testingBiologicalsMay 3, 2023Megan H. Cleveland, Erica L. Romsos, Carolyn R. Steffen, Nathan D. Olson, Stephanie L. Servetas, William G. Valiant, Peter M. Vallonefragments, a double restriction digest was performed with KasI (New England Biolabs, Ipswich, MA, catalog #R0544L) and NruI (New England Biolabs, catalog #R0192L) to cut the desired construct from the backbone plasmid.

(7) Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesMicrobiology SpectrumDecember 21, 2022Chengjin Ye, Luis Martinez-SobridoR0104L), isopropanol (Fisher Scientific, catalog no. BP26181), KasI (New England Biolabs, catalog no. R0544L), Luria-Bertani (LB) agar (BD, catalog no. 214010), Lipofectamine 2000 reagent (Thermo Fisher Scientific,

(8) ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in YeastmSphereOctober 26, 2022Akhuli D, Dhar A, Viji AS, Bhojappa B, Palani S.the genomic leu2 locus, piSP522, piSP523, piSP524, and piSP525 were linearized with KasI (catalog no. R0544; New England BioLabs) and transformed into the above-described strains as appropriate. To construct

(9) Chimeric Phi29 DNA polymerase with helix–hairpin–helix motifs shows enhanced salt tolerance and replication performanceMicrobial BiotechnologyJuly 1, 2021Yaping Gao, Yun He, Liyi Chen, Xing Liu, Igor Ivanov, Xuerui Yang, Hui Tian(residues 518–964) of Topo V after PCR were tethered to the COOH terminus of Phi29 Pol using KasI (R0544S; NEB, USA). The sequence of the linker peptide was GTGSGA. The scheme for generating the chimeric gene

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KasI

KasI from New England Biolabs

Sequence

New England Biolabs

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KasI from New England Biolabs

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