Caspase-3 Control Cell Extracts from Cell Signaling Technology

Product Specs
ItemCaspase-3 Control Cell Extracts
CompanyCell Signaling Technology
Price $162.00 Inquire
Catalog Number9663S
Quantity100 µl
SourceCell
Research AreaApoptosis
TypeCell Extracts
ReactivityH
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Unlocking microglia pyroptosis in a model of type I interferon-driven neuroinflammation: lessons from Rnaset2−/− miceCell Death & DiseaseDecember 27, 2025Kristin Wendland, Milena Irsfeld, Kathrin Schreiber, Katharina Ternka, Christine Stadelmann, Stefan Nessler, Jutta Gärtner, Matthias Kettwigwere applied in 5% non-fat milk in TBST at 4 °C overnight (Table 2). Caspase 3 control cell extracts (#9663, Cell Signaling, Danvers, MA, USA) were used as positive controls (20 µg per lane). Loading controls

(2) Boron‐Enhanced Mitochondrial Repair: DeepA‐I Tissue RegenerationScientifica (Cairo)October 26, 2025Beyza Yılmaz, Basak Dalbayrak, Mustafa Kucukali, Pinar Uysal Onganer, Elif Damla ArısanChemiDoc, Bio-Rad, US. For the Western blotting experiment, β-actin (#4967), AKT (#9272), Caspase-3 (#9663), Nrf2 (#20733), PARP (#9532), and HRP-linked secondary antibody (#7074) were purchased from Cell Signalling

(3) DNA methyltransferase 1 regulates epithelial cell functions in corneal and eyelid developmentMol VisMarch 28, 2025Antonius Christianto, Maureen Mongan, Bo Xiao, Qin Wang, Alvaro Puga, Michael L. Robinson, Ying XiaAd-Cre infection as described above were examined by immunofluorescent staining with anti-Caspase-3 (CST 9663S, Cell Signaling Technology, Danvers, MA) as the primary antibody, Alexa Fluor™ 594 Goat anti-Rabbit

(4) Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle productionStem Cell Res TherMarch 5, 2024Alexander Otahal, Karina Kramer, Markus Neubauer, Slavomira Gulová, Zsombor Lacza, Stefan Nehrer, Andrea De De Lunacontaining cleaved caspase 3 as a result of cytochrome C treated Jurkat cell extract (Cell Signaling, #9663) clearly shows cleaved caspase 3, which is not present in the HFP-MSC lysates neither before or after

(5) Repurposing AZD5438 and Dabrafenib for Cisplatin-Induced AKIJournal of the American Society of Nephrology : JASNJanuary 1, 2024Pushpan CK, Kresock DF, Ingersoll MA, Lutze RD, Keirns DL, Hunter WJ, Bashir K, Teitz T.PC-PMLKL, FabGennix International Inc, and Caspase-3 Control, Cell Extracts Cell Signaling Technology 9663, were used. Statistical Analysis The results are expressed as mean±SEM using Graph Pad Software, *P

(6) Comparison between articular chondrocytes and mesenchymal stromal cells for the production of articular cartilage implantsFrontiers in bioengineering and biotechnologyJanuary 1, 2023Frerker N, Karlsen TA, Stensland M, Nyman TA, Rayner S, Brinchmann JE.−20°C until analysis were performed. 20 µL of cell lysates and 10 µL of Caspase-3 control extracts (#9663; Cell Signalling Technology) were loaded onto 4%–20% gradient polyacrylamide gels (Bio-Rad). Proteins

(7) Effects of organic chemicals from diesel exhaust particles on adipocytes differentiated from human mesenchymal stem cellsBasic & Clinical Pharmacology & ToxicologyJanuary 1, 2023Bendik C. Brinchmann, Jørn A. Holme, Nadine Frerker, Mia H. Rambøl, Tommy Karlsen, Jan E. Brinchmann, Alena Kubátová, Klara Kukowski, Tonje Skuland, Johan ØvrevikSamples were stored at −20°C until analysis was performed. Cell lysates and caspase‐3 control extracts (#9663; Cell Signalling Technology) were loaded onto 4%–20% gradient polyacrylamide gels (Bio‐Rad). Proteins

(8) Evidences for Mutant Huntingtin Inducing Musculoskeletal and Brain Growth Impairments via Disturbing Testosterone Biosynthesis in Male Huntington Disease AnimalsCellsNovember 25, 2022Libo Yu-Taeger, Arianna Novati, Jonasz Jeremiasz Weber, Elisabeth Singer-Mikosch, Ann-Sophie Pabst, Fubo Cheng, Carsten Saft, Jennifer Koenig, Gisa Ellrichmann, Taneli Heikkinen, et al.analyses, a commercial sample of cell extracts treated with cytochrome c served as positive control (9663, Cell signaling). All fluorescence signals were detected and quantified using the ODYSSEY FC Imaging

(9) p53-mediated neurodegeneration in the absence of the nuclear protein Akirin2iScienceFebruary 18, 2022Stacey L. Peek, Peter J. Bosch, Ethan Bahl, Brianna J. Iverson, Mrutyunjaya Parida, Preeti Bais, J. Robert Manak, Jacob J. Michaelson, Robert W. Burgess, Joshua A. Weinerrespectively, for LC3 probe. Cytochrome c treated or untreated Jurkat cells (Cell Signaling Technology #9663) were used as positive and negative controls, respectively, for cleaved caspase 3 probe. Membranes were

(10) Pulmonary inflammatory response and immunomodulation to multiple trauma and hemorrhagic shock in pigsPloS oneJanuary 1, 2022Oestreich MA, Seidel K, Bertrams W, Müller HH, Sassen M, Steinfeldt T, Wulf H, Schmeck B.Health, USA) and normalized to ß-actin. Untreated and cytochrome c-treated Jurkat control cell extracts (#9663, Cell Signaling Technology, Frankfurt, Germany) served as controls. Primary monoclonal antibodies for

(11) circ-NOL10 regulated by MTDH/CASC3 inhibits breast cancer progression and metastasis via multiple miRNAs and PDCD4Molecular therapy. Nucleic acidsDecember 3, 2021Cai Y, Zhao X, Chen D, Zhang F, Chen Q, Shao CC, Ouyang YX, Feng J, Cui L, Chen M, et al.anti-cleaved caspase-3 antibodies were purchased from Cell Signaling Technology (catalog numbers 2118S, 9663S, and 9661S, respectively). TUSC2 antibodies were purchased from Affinity Bioscience (catalog number

(12) Mycobacterium tuberculosis Exploits Focal Adhesion Kinase to Induce Necrotic Cell Death and Inhibit Reactive Oxygen Species ProductionFrontiers in ImmunologyOctober 20, 2021Afrakoma Afriyie-Asante, Ankita Dabla, Amy Dagenais, Stefania Berton, Robin Smyth, Jim SunWestern blot analysis was performed according to standard protocols. The Caspase-3 Control Cell Extracts (#9663) and the primary rabbit antibodies to FAK (#13009S), cleaved caspase 3 (#9661S), and total caspase 3

(13) Cryptotanshinone, a novel PDK 4 inhibitor, suppresses bladder cancer cell invasiveness via the mTOR/β‑catenin/N‑cadherin axisInternational journal of oncologyJuly 1, 2021Kim CJ, Terado T, Tambe Y, Mukaisho KI, Kageyama S, Kawauchi A, Inoue H.donkey) secondary antibodies were purchased from Cytiva. Caspase-3 control cell extracts (cat. no. #9663) for the negative and positive controls for apoptosis analysis were purchased from Cell Signaling Technology,

(14) LncRNA PART1 Promotes Proliferation and Migration, Is Associated with Cancer Stem Cells, and Alters the miRNA Landscape in Triple-Negative Breast CancerCancers (Basel)May 27, 2021Brianne M. Cruickshank et al.temperature. The membrane was incubated with cleaved caspase-3 (Asp175, 5A1E) rabbit monoclonal antibody (#9663, Cell Signaling, 1:1000 in 5% BSA) overnight at 4 °C followed by peroxidase affiniPure goat anti-rabbit IgG (H + L

(15) Mitochondrial Transplantation Modulates Inflammation and Apoptosis, Alleviating Tendinopathy Both In Vivo and In VitroAntioxidants (Basel, Switzerland)April 28, 2021Lee JM, Hwang JW, Kim MJ, Jung SY, Kim KS, Ahn EH, Min K, Choi YS.1 (MMP1) (Santa Cruz; sc-58377), p-NF-kB p65 (Ser536), NF-kB p65 (Cell Signaling, Danvers, MA, USA; 9663S), anti-Fis1 (Santa Cruz; sc-376446), anti-Drp1 (Abcam; ab56788), anti-Mitofusin 2 (MFN2) (Abcam; ab56889),

(16) Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysisScience advancesSeptember 1, 2020Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, et al.Cell Signaling Technology (Jurkat cells and Jurkat cells incubated with cytochrome c control cell extracts, #9663; 10 μl loaded per well). ANXA1 EV surface localization was assessed by isolating human SMC and VIC EVs.

(17) Oxymatrine Inhibits Transforming Growth Factor β1 (TGF-β1)-Induced Cardiac Fibroblast-to-Myofibroblast Transformation (FMT) by Mediating the Notch Signaling Pathway In VitroMedical science monitor : international medical journal of experimental and clinical researchSeptember 9, 2018Zhao L, Xu Y, Tao L, Yang Y, Shen X, Li L, Luo P.

(18) Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius musclePloS oneJanuary 1, 2018Lynch JM, Dolman AJ, Guo C, Dolan K, Xiang C, Reda S, Li B, Prasanna G.and transgenic mice. Control cell extracts for CASP-3 (10μL per well) were from Cell Signaling Technology (9663). Controls for CASP-12 were cell lysates (40μg lysates/well) obtained from HeLa cells +/- 1μM staurosporine

(19) DNA methyltransferase 3a modulates chemosensitivity to gemcitabine and oxaliplatin via CHK1 and AKT in p53‑deficient pancreatic cancer cellsMolecular medicine reportsJanuary 1, 2018Jing W, Song N, Liu Y, Qu X, Hou K, Yang X, Che X.p-extracellular signal-regulated kinase (ERK)1/2 (Thr202/Tyr204; cat. no. 4370S), Caspase-3 (cat. no. 9663S) and γ-histone H2AX (γ-H2AX; cat. no. 9718S) were obtained from Cell Signaling Technology, Inc. (Danvers,

(20) C-Kit Cardiac Progenitor Cell Based Cell Sheet Improves Vascularization and Attenuates Cardiac Remodeling following Myocardial Infarction in RatsBioMed research internationalJanuary 1, 2018Dergilev K, Tsokolaeva Z, Makarevich P, Beloglazova I, Zubkova E, Boldyreva M, Ratner E, Dyikanov D, Menshikov M, Ovchinnikov A, et al.

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